• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.368.2-368.2
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• 2002

Fruits of Hovenia dulcis T. (Rhamnaceae) was called ′jiguja ′ in oriental medicine which has been used for diuresis, remove of hangover and leaves has been used for detoxified the alcohol. From the MeOH Extraction. five compounds were isolated by column chromatography and elucidated as quercetin quercetin-3-O-rhamnose. quercetin-3-O-gal(6"1") rha, quercetin-3-O-glc(6"${\rightarrow}$1")glc, and kaemferol through specroscopic methods. (omitted)

• Biotechnology and Bioprocess Engineering:BBE
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• 제4권1호
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• pp.26-31
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• 1999

The advantages of the organism Streptomycs griseus HUT 6037 is that the chitinase and chitosanase using chitinaceouse substrate are capable of hydrolyzing both amorphous and crystalline chitin and chitosan. We attempted to investigate the optimization of induction protocol for high-level production and secretion of chitosanase and the influence of chitin and partially deacetylated chitosan sources (75∼99% deacetylation). The maximum specific activity or chitinase has been found at 5 days cultivation with the 48 hours induction time using colloidal chitin as a carbon source. To investigate characteristic of chitosan activity according to substrate, we used chitosan with various degree of deacetylation as a carbon source and found that this strain accumulates chitosanase in the culture medium using chitosanaceous substrates rather than chitinaceous substrates. The highest chitosanase activity was also presented on 4 days with 99% deacetylated chitosan. The partially 53% deacetylated chitosan can secrete both chitinase and chitosanase which was defined as a soluble chitosan. The specific activities of chitinase and chitosanase were 0.89 at 3 days and 1.33 U/mg protein at 5 days, respectively. It indicate that chitosanase obtained from S. griseus HUT 6037 can hydrolyze GlcNAc-GlcN and GlcN-GlcN linkages by exo-splitting manner. This activity increased with increasing degree of deacetylation of chitosan. It is the first attempted to investigate the effects of chitosanase on various degrees of deacetylations of chitosan by S. griseus HUT 6037. The highest specific activity of chitosanase was obtained with 99% deacetylated chitosan.

• 한국수산과학회지
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• 제28권2호
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• pp.131-136
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• 1995

조피볼락, 잉어 및 무지개송어의 혈청을 상온$(15^{\circ}C)$ 저온$(4^{\circ}C)$, 냉동$(-20{\circ}C)$으로 보존하면서 총단백질(TP), 알부민(ALB), 중성지방(TG), 콜레스테롤(CHOL), 포도당(GLC), 인(P), 나트륨(Na), AST 및 ALT의 활성 변화를 상온과 저온에서는 16일까지, 냉동에서는 30일까지 조사하였다. 어종간에 다소의 차이가 있기는 하지만 대체로 단백질 성분인 TP, ALB과 당 성분인 GLC, 무기질인 P, Na의 농도는 안정하였고, 지방 성분인 TG와 CHOL, AST, ALT의 활성은 불안정하였다 그리고 온도에 따른 혈액 성분의 안정성은 상온에서보다는 저온과 냉동에서 대체로 안정하지만, 성분에 따라서는 냉동하여도 매우 불안정한 것이 있다

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.454-460
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• 2007

Enzymatic properties and substrate specificity of recombinant $\beta-glycosidases$ from a hyperthermophilic archaeon, Sulfolobus shibatae (rSSG), were analyzed. rSSG showed its optimum temperature and pH at $95^{\circ}C$ and pH 5.0, respectively. Thermal inactivation of rSSG showed that its half-life of enzymatic activity at $75^{\circ}C$ was 15 h whereas it drastically decreased to 3.9 min at $95^{\circ}C$. The addition of 10 mM of $MnCl_2$ enhanced the hydrolysis activity of rSSG up to 23% whereas most metal ions did not show any considerable effect. Dithiothreitol (DTT) and 2-mercaptoethanol exhibited significant influence on the increase of the hydrolysis activity of rSSG rSSG apparently preferred laminaribiose $(\beta1\rightarrow3Glc)$, followed by sophorose $(\beta1\rightarrow2Glc)$, gentiobiose $(\beta1\rightarrow6Glc)$, and cellobiose $(\beta1\rightarrow4Glc)$. Various. intermolecular transfer products were formed by rSSG in the lactose reaction, indicating that rSSG prefers lactose as a good acceptor as well as a donor. The strong intermolecular transglycosylation activity of rSSG can be applied in making functional oligosaccharides.

• 분석과학
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• 제9권2호
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• pp.123-133
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• 1996

기체 크로마토그래피법을 이용하여 과수 재배용 살균제인 difenoconazole의 분석을 위한 최적 조건을 구하고, 사과와 토양 중에서의 잔류성을 조사하였다. 사과사료는 5% NaCl과 n-hexane액으로 분리, 농축하여 florisil column상에서 acetone과 n-hexane 혼합액으로 정제하여 GLC(ECD)로 분석하였다. 0.20과 1.0ppm의 표준물 첨가실험결과 평균회수율은 86.0~92.0% 였고, 검출한계는 0.01ppm 이었다. 사과에 대한 안정 사용기간은 수확 15일 전 3회 사용이 적당한 것으로 나타났으며, 이 경우 difenoconazole의 잔류량은 0.037ppm에서 0.044ppm 이었다. 토양시료를 methanol과 ammonium hydroxide 의 혼합액으로 추출하여 여과한 후 포화 NaCl 용액과 dichloromethane층으로 분배하였다. 유기층을 농축하여 toluene 용매로 재용해하였고, Sep-Pak column으로 정제한 후 GLC(FID)로 분석하였다. 0.10, 0.50과 1.0ppm 표준물 첨가실험 결과 평균 회수율은 101.2~103.7% 였고, 검출한계는 0.025ppm 이었다.

• 한국수산과학회지
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• 제33권2호
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• pp.119-123
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• 2000

은연어가 운반과정 중에 어느 정도 스트레스를 받는지 조사하기 위하여 어류를 각각 담수와 염수 ($5{\%{\circ}}$)를 채운 수조차에 싫고 10 시간 운반하였다. 운반하기 전후에 혈청 중의 1차 스트레스 지표 물질인 코티졸을 비롯하여 2차 지표물질인 GLC, LAC, TG, CHOL, 전해질 ($Na^+, Cl^-$), 효소 (ALT, AST, LDH) 활성을 측정하여 두 운반구 간의 차이를 비교하였다. 그 결과, 운반작업은 은연어에게 상당한 스트레스를 주는 것으로 확인되었으나, 운반방법에 따라서는 스트레스 반응에 차이가 있었다. 즉, 담수운반구에 비해 염수운반구는 코티졸, GLC, LAC, TG, CHOL, AST 및 ALT가 유의적인 차이를 보이며 낮은 수준이었다. 이것으로 은연어의 운반시에는 담수보다 염수를 사용하는 것이 스트레스를 줄일 수 있다는 것을 알 수 있었다.

• Parasites, Hosts and Diseases
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• 제32권2호
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• pp.101-110
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• 1994

심장사상충 자충이 합성한 당단백의 분지(분지) 역할을 하는 N-linked high mannose 타입 올리고당의 구조에 대한 조사를 수행하였다. 사상충 자충을 방사선 표식 2-[$^3H$] mmannose를 함유한 배지에서 24시간 배양하였다. 자충으로부터 분리 정제한 당단백을 pronate로 소화시킨 다음. concanavalin A-Sepharose 크로마토그라피하여 분획하였다. 37%의 mannose가 자충 대사에 이용되었으며 pectin chromatography를 사용하여 high mannose 타입의 올리고당을 회수하였다. 이 올리고당을 $endo-{\beta}-N-acetylglucosaminidase$ H 효소로 소화시킨 후 HPLC를 사용하여 highmannose 타입 올리고당의 구성을 분석하였다. 심장사상충 자충이 합성한 high mannose 타입 올리고당의 형태는 $Man_5GIcNAc_2,{\;}Man_6GIcNAc_2,{\;}Man_7GIcNAc_2과{\;}Man_8GlcNAc_2$로 확인되었다. 이와 같이 심장사상충 자충이 체내 합성한 high mannose 타입 올리고당이 일반 척추동물이 체내 합성한 high mannose 타입 올리고당과 구조적으로 일치하였다.

• 한국식품조리과학회지
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• 제6권2호
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• pp.37-50
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• 1990

The cause and the degree of the cis to trans isomerization were investigated about soybean oil (SBO), corngerm oil (CGO), cottonseed oil (CSO), margarine (MG) and shortening (ST). All samples treated with various conditions were analyzed to determine physicochemical characteristics (AV, POV, IV, RI), fatty acid composition, total trans fatty acid content and change of trans fatty acid composition by GLC, IR and HPLC. The results were obtained as follows; 1. Physicochemical constants were changed with a gentle slope according to incubating period at 40${\pm}$2$^{\circ}C$ and physicochemical constants of margarine and shortening were changed, significiantly. 2. The saturation degree in the unsaturated fatty acid composition determined by GLC gradually were increased during incubation and heating periodically. For palmitic-and stearic acid content at the samples stored in the incubator, the saturation degrees were gradually increased. But for the case of heat treatment, they were increased more rapidly than other fatty acids. 3. Total trans fatty acid contents in each samples were determined by GLC, IR and HPLC, the amount of trans fatty acids were measured with discrepancy. It was caused by deviation of analytical instruments, methods and the kinds of samples. Trans fatty acids were measured more definitly in IR more than GLC and HPLC. On the other hand, total trans fatty acid contents in average levels for SBO, CGO, CSO, MG and ST stored for 35 days and heated for 24 hours were 1.3%, 1.1%, 0.9%, 22.6% and 13.8%, and 3.6%, 3.0%, 2.8%, 41.2% and 20.8%, respectively.

• BMB Reports
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• 제44권6호
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• pp.415-420
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• 2011

Diabetes is a well-known independent risk factor for vascular disease. However, its underlying mechanism remains unclear. It has been reported that increased influx of the hexosamine biosynthesis pathway (HBP) induces O-GlcNAcylation of proteins, leading to insulin resistance. In this study, we determined whether or not O-GlcNAc modification of proteins could increase vessel contraction. Using an endothelium-denuded aortic ring, we observed that glucosamine induced OGlcNAcylation of proteins and augmented vessel contraction stimulated by U46619, a thromboxane $A_2$ agonist, via augmentation of the phosphorylation of MLC20$MLC_{20}$, MYPT1(Thr855), and CPI17, but not phenylephrine. Pretreatment with OGT inhibitor significantly ameliorated glucosamine-induced vessel constriction. Glucosamine treatment also increased RhoA activity, which was also attenuated by OGT inhibitor. In conclusion, glucosamine, a product of glucose influx via the HBP in a diabetic state, increases vascular contraction, at least in part, through activation of the RhoA/Rho kinase pathway, which may be due to O-GlcNAcylation.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.582-588
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• 1999

A Brevibacterium lactofermentum gene coding for a glucose-specific permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned, by complementing an Escherichia coli mutation affecting a ptsG gene with the B. lactofermentum genomic library, and completely sequenced. The gene was identified as a ptsG, which enables an E. coli transformant to transport non-metabolizable glucose analogue 2-deoxyglucose (2DG). The ptsG gene of B. lactofermentum consists of an open reading frame of 2,025 nucleotides encoding a polypeptide of 674 amino acid residues and a TAA stop codon. The 3' flanking region contains two stem-loop structures which may be involved in transcriptional termination. The deduced amino acid sequence of the B. lactofermentum enzyme $II^{GIe}$ specific to glucose ($EII^{GIe}$) has a high homology with the Corynebacterium glutamicum enzyme $II^{Man}$ specific to glucose and mannose ($EII^{Man}$), and the Brevibacterium ammoniagenes enzyme $II^{GIc}$ specific to glucose ($EII^{GIc}$). The 171-amino-acid C-terminal sequence of the $EII^{Glc}$ is also similar to the Escherichia coli enzyme $IIA^{GIc}$ specific to glucose ($IIA^{GIc}$). It is interesting that the arrangement of the structural domains, IIBCA, of the B. lactofermentum $EII^{GIc}$ protein is identical to that of EIIs specific to sucrose or $\beta$-glucoside. Several in vivo complementation studies indicated that the B. lactofermentum $EII^{Glc}$ protein could replace both $EII^{ Glc}$ and $EIIA^{Glc}$ in an E. coli ptsG mutant or crr mutant, respectively.