• 대한수의학회지
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• 제32권1호
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• pp.127-134
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• 1992

Present experiments were undertaken in order to clarify the clinico-pathological characteristics of lead poisoning in goats. Twenty goats were divided into three experimental groups(A, B and C) and a control(D). The three experimental groups received diets contaminated artificially with 10(A group), 200(B group) and 1,000(C group) ${\mu}g/$ of lead, for 70 days respectively. The control group received normal diets. Blood samples were collected 1 or 2 weeks interval and were examined for anemia(erythrocyte counts, hemoglobin concentrations and hematocrit values) and lead contents of erythrocyte and serum. Urine samples collected similarly with blood were examined for delta-aminolevulinic acid and lead content. Collected samples were analyzed for lead content by atomic absorption spectrophotometry. From these experiments following results were obtained : In group B and C, marked decreases in body weight and feed intake, and diarrhea were observed from the $30^{th}$ day of experimental periods. The B and C groups showed pronounced anemia(decrease in erythrocyte count, hemoglobin concentration and hematocrit value) from the $21^{st}$ or $42^{nd}$ day. In group B and C, the lead content of erythrocytes was increased significantly from the $28^{th}$ or $14^{th}$ day. The lead content of serum was increased significantly from the $42^{nd}$ or $14^{th}$ day in B and C groups. The lead content of urine was increased significantly from the first day in both B and C groups. The urinary delta-aminolevulinic acid content was increased significantly from $14^{th}$ day in both B and C groups.

• 대한조선학회논문집
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• 제53권2호
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• pp.127-134
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• 2016

This paper presents practical issues for test and evaluation(T&E) methods to develop defense systems. Our argument is motivated by several domestic defense cases and the cases lead us to discuss two main factors for reliable defense systems development: 1) statistical approaches and 2) technical schemes. Specifically, statistical approaches enable to provide credible interpretations about T&E results in the decision-making process. With practical T&E results of the “Red Shark” torpedo, we performed statistical hypothesis tests and suggest a minimum sample size to accept the hypothesis. Next, technical schemes have more direct effects on improving reliability of developed defense systems and we shortly introduce tools development for systems verification that is required to integrate several sub-systems, e.g., combat, sensor, weapon, and communication systems, within a defense system. We additionally summary some domain cases using modeling and simulation techniques for successful T&E. In closing, we expect that the paper shows empirical investigation and lessons learned with these two practical issues, which provides a guide those who desire to make decisions about reliable defense systems development.

• Molecules and Cells
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• 제42권2호
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• pp.123-134
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• 2019

Lysophosphatidic acid (LPA) is an endogenous lysophospholipid with signaling properties outside of the cell and it signals through specific G protein-coupled receptors, known as $LPA_{1-6}$. For one of its receptors, $LPA_1$ (gene name Lpar1), details on the cis-acting elements for transcriptional control have not been defined. Using 5'RACE analysis, we report the identification of an alternative transcription start site of mouse Lpar1 and characterize approximately 3,500 bp of non-coding flanking sequence 5' of mouse Lpar1 gene for promoter activity. Transient transfection of cells derived from mouse neocortical neuroblasts with constructs from the 5' regions of mouse Lpar1 gene revealed the region between -248 to +225 serving as the basal promoter for Lpar1. This region also lacks a TATA box. For the region between -761 to -248, a negative regulatory element affected the basal expression of Lpar1. This region has three E-box sequences and mutagenesis of these E-boxes, followed by transient expression, demonstrated that two of the E-boxes act as negative modulators of Lpar1. One of these E-box sequences bound the HeLa E-box binding protein (HEB), and modulation of HEB levels in the transfected cells regulated the transcription of the reporter gene. Based on our data, we propose that HEB may be required for a proper regulation of Lpar1 expression in the embryonic neocortical neuroblast cells and to affect its function in both normal brain development and disease settings.

• 대한통합의학회지
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• 제10권2호
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• pp.125-134
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• 2022

Purpose : Establishing multicultural acceptance is important to provide quality nursing care in various clinical settings and adapt to changing roles in different situations. The purpose of this study was to explore degree of intercultural sensitivity, cultural empathy, and multicultural acceptance, the factors influencing of multicultural acceptance of nursing students and to provide the basic data to increased multicultural acceptance. Methods : The study used a questionnaires that include general characteristics, multicultural acceptance, intercultural sensitivity, cultural empathy for nursing students. This research involved 173 nursing students in G city. The data collected from August 15, 2021 to August 30, 2021. The study was analyzed using the SPSS 23.0 program. The correlation among intercultural sensitivity, cultural empathy, and multicultural acceptance was analyzed by Pearson's correlation coefficients, and it was analyzed using multiple regression to figure out factors influencing multicultural acceptance. Results : The multicultural acceptance score was above the middle level and differed according to the academic grade (F=8.580, p<.001), major satisfaction (F=5.981, p=.003), interpersonal relations significantly (F=6.837, p=.001). Multicultural acceptance was a significant positive correlation with intercultural sensitivity (r=.544, p<.001), cultural empathy (r=.624, p<.001). As a result of regression analysis, it was confirmed that cultural empathy (β=.477, p<.001) and intercultural sensitivity (β=.212, p<.001) were factors that influence nursing students' multicultural acceptance ; this model could explain 40 %. Conclusion : Factors affecting multicultural acceptance of nursing students are cultural empathy and intercultural sensitivity. Nursing students are encouraged to have multicultural acceptance in terms of satisfaction of cultural empathy and intercultural sensitivity. Through this research requires the fellow study to determine the various factors affecting multicultural acceptance of nursing students. And it is necessary to development education and program that can enhance multicultural acceptance is required.

• 한국수산과학회지
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• 제52권2호
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• pp.134-140
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• 2019

A feeding trial was conducted to investigate the effects of dietary fish meal replacement by a blend of lysine cell mass, corn protein concentrate and poultry by-product meal on the growth and blood chemistry of the starry flounder Platichthys stellatus. The fish meal replacer (FMR) was prepared to have the same level of protein as fish meal (FM). With a commercial diet as a positive control, five experimental diets (basal, FM42, FM32, FM22 and FM12) were formulated to contain 52% protein and 10% lipid. The dietary FM levels decreased from 52% (basal) to 42, 32, 22 and 12% with concomitant increase in the FMR to 10, 20, 30, 40 and 50%, respectively. Juvenile starry flounder with an average body weight of 177.3 g were randomly distributed in each (30 fish/tank) of 18 plastic tanks ($139{\times}99{\times}54cm$). After a 45-day feeding trial, the survival rate ranged from 95.6% (FM22) to 100% (control and FM42), while the weight gain of the fish groups varied from 49.7 to 58.4 g. The results clearly revealed that starry flounder can grow well on a diet containing low FM (12%) with a high level of FMR (50%) without any adverse effects.

• 한국작물학회지
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• 제46권6호
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• pp.453-458
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• 2001

강원도 지역에 적응성이 있는 품종을 선정하기 위해서 5개 시험지에서 11개 품종으로 1997년부터 2000년 동안에 수행된 결과를 분석하였다. 품종이 가지고 있는 유전적 특성은 환경요인과의 상호작용을 거쳐 발현되므로 장려품종을 선정하기 위하여 수행하는 다지역검정에서 이 유전자-환경간 상호작용을 평가하는 것은 품종의 환경안정성과 지역적응성을 올바르게 파악하는 데 매우 중요하다. 이를 위해서 기존에 활용되어 온 회귀분석모델로서 안정성을 분석하였고 AMMI model로서 지역별 수량성 반응의 변화양상을 토대로 유전자-환경간간의 상호작용을 해석하면서 각 시험지별 적응성 품종과 지역적 특성을 검토하였다. 1. 각 시험지에 대한 수량안정성 평가를 위한 회귀분석 결과 오봉벼, 진미벼, 신운봉벼, 운봉벼 등이 안정성이 있는 품종이었다. 2. AMMI 분석에 의한 수량성에 대한 분산분석 결과 전 변이중 품종변이가 66％, 품종-환경간 상호작용변이가 21％, 지역간 변이가 13％였다. 21％의 품종-환경간 상호작용 변이는 주성분분석을 통하여 제1주성분치(IPCAl)와 제2주성분치 (IPCA2)에 의해서 거의(92％) 해석되었다. 3. 품종들의 지역간 수량성 변화패턴은 육성모지나 시험지 등에 영향을 받았고, 5개 시험지의 수량변화 양상은 철원과 정선, 춘천과 고성이 비슷하였고 강릉은 다르게 구분되었다. 품종-환경간 상호작용 양상이 축약된 주성분분석치와 시험지별 재배기간동안의 순별 평균 기상자료와의 상관분석 결과를 기준으로 품종과 환경간의 상호작용에 관여하는 주요 기상요인은 초상최저온도, 최소상대습도, 일조시수 강수량 등이었다. 4. 비교적 환경안정성이 있는 품종으로는 오대벼, 오봉벼, 진부벼 등이었다. 철원에 적응성이 있는 품종은 진부벼, 신운봉벼, 오대벼, 오봉벼 등에었고, 춘천에는 진미벼 상주벼, 오대벼, 오봉벼가 정선, 강릉, 고성에는 진부찰벼, 삼천벼, 둔내벼 , 운봉벼, 진부올벼가 적응성이 있었다. 0.57, Fe 0.414, p 94.68, fat 3.74, protein 3.08, lactose 4.68,4. In case of processed market cow′s milk ; Ca 134.72, K 142.74, Mg 10.33, Na 45.07, Zn 0.50, Fe 0.650, p 92.48, fat 3.72, protein 3.07, lactose 4.74. According to the group of market milk(milk, fortified market row′s milk, processed market cow′s milk), the mean concentration of Ca and Fe were significantly higher in fortified and processed milk than milk(p<0.05). There were no significant differences in macronutrient(fat, protein, lactose) and mineral contents between pasteurized milk and UHT(ultra high temperature) treated milk($\alpha$=0.05). The labeled "Nutritional Facts" of market milk were satisfied with "Labeling Standards for Livestock Products of Korea". The measured mean concentrations of Ca, Fe, Zn were generally higher than lower limit of labeled value(above 80% of labeled value). The mean concentration of sodium was lower than upper limit of labeled value(below 120％

• 한국토양비료학회지
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• 제30권2호
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• pp.129-134
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• 1997

전북(全北) 봉동 재래종인 생강(生薑)을 공시(供試)하여 송정미사질양토(松汀微砂質壤土)에서 pot시험(試驗)으로 토양수분함량에 따른 생강의 생육(生育)을 조사(調査)한 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 생강출아는 토양함수량 20%(g/g) 이상 처리구에서 파종 20~25일에 80~100%의 출아율을 보였다. 2. 생강지상부의 경장, 경태, 경수, 엽장, 엽폭 등은 토양함수량 20~25% 처리구에서 가장 양호하였다. 3. 생강지상부의 고사율은 토양함수량 25% 처리구를 기점으로 많거나 적으면 증가하는 경향이었다. 4. 생강 지하부 생육 토양함수량 25%처리구에서 가장 양호하였다. 5. 생강 부패율은 토양함수량 20~25% 처리구에서 가장 낮았다. 6. 생강 수량과 경태($r=0.971^{**}$), 경수($r=0.980^{**}$) 간에 각각 고도(高度)의 정(正)의 상관(相關)을 보였다.

• 치위생과학회지
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• 제15권4호
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• pp.424-429
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• 2015

본 연구는 trtiptolide의 안면부 통증 조절효과를 평가하기 위하여 포르말린으로 유도된 실험동물의 안면부 통증모델에서 말초와 중추로 투여한 trtiptolide의 농도별 차이를 확인하였고, 다음과 같은 결과를 얻었다. 먼저, 안면부 피하로 triptolide 투여 후 포르말린 주입군에서 포르말린 주입 후 11~45분에 발생하는 2차 통증행위반응은 25, $50{\mu}g/50{\mu}l$의 각 농도에 따라 $179.17{\pm}15.36$회, $114.33{\pm}16.65$회로 나타났고 $299.00{\pm}28.14$회로 나타난 포르말린 주입군과 비교시 통증행위반응이 유의하게 경감되었다. 시간의 경과에 따른 변화에서 $50{\mu}g/50{\mu}l$의 투여군에서 포르말린 주입 후 20분, 30분에 통증행위반응이 포르말린군과 비교하여 유의하게 감소되었음을 확인하였다. 안면부 피하로 투여한 triptolide는 포르말린으로 유도된 통증행위반응을 감소시킴으로써 안면부 통증조절효과를 나타내었다. 다음으로, 소뇌연수조 내로 triptolide 투여 후 포르말린 주군에서 2차 통증행위반응은 $1{\mu}g/10{\mu}l$의 $134.00{\pm}23.03$회로 나타났고, $289.50{\pm}44.30$회로 나타난 포르말린군과 비교 시 통증행위 반응이 경감되었다. 시간의 경과에 따른 변화에서, $1{\mu}g/10{\mu}l$의 triptolide 투여군에서 포르말린 주입 후 25~40분에 통증행위반응이 감소되었음을 확인하였다. 소뇌연수조로 투여한 triptolide는 포르말린으로 유도된 통증행위반응을 감소시킴으로써 안면부 통증조절효과를 나타내었다. 이상의 결과는, triptolide는 구강 및 턱관절 등을 포함하는 안면부 통증 조절의 예방 및 치료에 있어 활용 가능한 천연대체 약물로 제시될 수 있을 것으로 생각된다.

• 한국융합학회논문지
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• 제10권12호
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• pp.129-134
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• 2019

의료기관에서 많이 사용되는 방사선 차폐 재료인 납을 대처할 수 있는 플라스틱 제품으로 PVC를 선택하였다. 방사선 차폐 의복 이외에 저선량 영역에서 의료기기 부품, 산업용 차폐 재료로 사용이 가능한지 평가하고자 한다. 상업용 PVC는 밀도가 3.68 g/㎠ 으로 재료의 유연성과 경제성 등 일정 방사선 영역에서는 충분한 차폐 효과를 긍정적으로 기대할 수 있으며, 다양한 형태로 변형이 가능하고, 경량의 차폐벽으로 사용할 수 있다. 의료기관에서 임상 검사 시 사용되는 의료방사선 영역 대에서 3mm PVC 5장을 두께로 조절하여 차폐 성능을 실험하였다. 의료방사선의 관전압 기준으로 실효에너지에 대한 차폐 성능을 평가하였다. PVC는 두께가 두꺼울수록 관전압과 실효에너지가 낮을수록 차폐효과는 크게 나타났으며, 차폐효과는 12mm 두께에 관전압 80kVp에서 70%의 차폐효과가 있었다. 따라서 PVC 재료의 차폐효과는 두께의 의존율이 높게 나타났다. 앞으로는 차폐 성능을 높이면서 얇고 가벼운 친환경 제품을 만들기 위한 지속적인 연구가 필요하다.

• The Plant Pathology Journal
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• 제17권5호
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• pp.300-304
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• 2001

Apple is the most economically important fruit in Korea. The suspected viroid disease of dapple apple was found in apple fruits cultivated in Kyungpook province. Symptoms begin in mid-July as small circular spots, which stand out against the background color on the young fruit. Dappling of the fruit becomes more intense and easier to detect as the fruit approaches maturity; the affected spots remain yellowish as the fruit matures. no leaf or bark syndromes have been associated with this disease. The infected fruits are downgraded considerably during quality grading. The low molecular weight RNA containing viroid RNA molecules were extracted from the peels of the apples with dapple symptoms. The RNA molecules were extracted from the apples using Qiagen column chromatography. The purified RNAs were used for the synthesis of cDNA with RT-PCR. The PCR products were then ligated into a pGEM-T Easy vector, cloned and sequenced. The sequence of the viroid RNA molecule shows 331 nucleotides with one base difference ("G" insertion between the position of 133 and 134) compared with that of the Apple scar skin viroid (ASSVd) reported by Hashimoto and Koganezawa in Japan. This is the first report on the occurrence of the ASSVd in apple trees cultivated in Korea, as well as the identification of a new Korean strain of the ASSVd.the ASSVd.