• 한국미생물·생명공학회지
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• 제21권1호
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• pp.82-87
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• 1993

한국 전통 발효식품의 숙성에 절대 혐기성 세균이 관여하는지를 보기 위해 김치류와 장류 14종의 시료를 복합 BL 배지 혹은 bifidus 세균 선택 배지인 BS agar에 접종하여 절대 혐기적으로 배양한 결과 복합 BL 배지에서 $10^7$ ~ $10^8$/g 시료의 세균이 생장하였으며, BS agar에서는 $10^3$ ~ $10^6$/g 시료의 colony가 형성되었다. 이들은 모두 호기성 조건에서도 생장 할 수 잇었으며, BS 배지에서 생장하는 세균은 fructose-6-phosphate phosphoketolase를 합성하지 않는 것으로 나타났다. 또한 표준 Bifidobacterium속 세균은 3% NaCl을 함유하는 배지에서는 생장할 수 없었다. 이상의 결과로부터 김치류와 장류 등 염 농도가 높은 발표식품에는 bifidus균은 생장할 수 없다고 판단된다.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.352-358
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• 1995

In this study, authors investigated the cadmium tolerance, the accumulation of cadmium, and the cellular distribution of accumlated cadmium in lactic acid bacteria under the anaerobic condition. Lactic acid bacteria grew fairly well in modified EG medium containing 10 ppm of cadmium but could hardly grow at 50 ppm of cadmium. Tolerance to cadmium of genus Lactobacillus was greater than that of genus Streptococcus, and Lactobacillus acidophilus showed the higest cadmium tolerance amomg the bacteria tested. The capacity of cadmium accumlation (9.304-12.428 mg/g wet cell) of lactic acid bacteria was higher than that (6.775 mg/g wet cell) of Escherichia coli. Lactobacillus casei of them took up the largest amount of cadmium. The cadmium elimination amount (28.46-29.25%) of lactic acid bacteria from modified EG medium containing cadmium were also higher than that (14.43%) of Escherichia coli. Accumulated cadmium in Lactobacillus acidophilus was distributed by 42.41% at cell wall, 28.97% at cytoplasm, and 28.62% at plasma membrane, respectively.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• 제2권2호
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• pp.99-108
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• 1998

Results obtained from this research showed that the anaerobic contact process was applicable to pear waste with COD removal efficiencies of up to $95\%$ depending on conditions, provided ammonium and phosphate salts were added as well as other nutrients, present in the commercial fertilizer, Milorganite or in yeast extract. These latter materials were required in minimum concentrations of 5 and 1.5 g/L, respectively, in the feed independent of HRT and volatile solids loading rate, with part of the effect due to the mineral fraction. Digestion was satisfactory over the whole range of volatile solids loading rates and liquid retention time of 30 to 0.5 days tested, although treatment efficiency dropped off noticeably between 1 and 0.5 day liquid retention time because of poorer flocculation and separation of anaerobic bacteria. Settling of anaerobic bacteria including methane producing bacteria was related to settling of mixed liquor suspended solids only at 1 to 5 days liquid retention times, at other liquid retention times anaerobic microorganism settled markedly less efficiently than mixed liquor suspended solids. Further studies are being made to provide information of practical and basic interest. Data on the composition of the active fraction of yeast extract might solve many practical nutrient problems encountered with the anaerobic contact process and improve its economics. Further improvement in the flocculation and settling of anaerobic bacteria as well as other bacteria would improve overall performance and allow the use of shorter liquid retention times with dilute waste. Knowledge about the numbers of methane formers present would allow a degree of understanding and control of the process not presently attainable.

• 자원리싸이클링
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• 제26권1호
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• pp.59-68
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• 2017

본 연구는 항진 항균 작용이 보고되어진 고춧가루 함량(0% ~ 1%)에 따른 음식물쓰레기와 하수슬러지 혼합 물질(1 : 5 V/V%)의 혐기성통합소화 효과를 평가하기 위하여 수행되었다. 본 연구에서 고춧가루 함량 변화가 혐기성미생물 중 가수분해균과 메탄생성균보다 산생성균에 영향을 더 큰 줄 수 있음을 보였으며, 고춧가루함량이 가장 낮은 CAP0 (Capsaicin 0)에서 실험적 누적 메탄수율(ECMY)과 실험적 바이오생산량(EBEP)이 각각 0.17 L $CH_4/g$ $VS_{fed}$와 1,465 cal/g $VS_{fed}$로 최고값으로 측정되어졌다. 그리고 고춧가루 함량이 증가함에 따라 정상운전 위하여 혐기성 미생물의 순응을 위한 기간이 필요함을 보였다.

• 한국환경과학회지
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• 제30권12호
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• pp.1093-1100
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• 2021

The present study investigated the effect of ammonia load on microbial communities in mesophilic anaerobic digestion of propionic acid. A laboratory-scale continuous anaerobic digester treating propionic acid as a sole organic substrate was operated under non-inhibitory condition and inhibitory conditions with ammonia (1.5 g and 3.5 g ammonia-N/L, respectively), and bacterial and archaeal communities in the steady states of each ammonia condition were analyzed using high-throughput sequencing. Thirteen bacterial families were detected as abundant bacterial groups in mesophilic anaerobic digestion of propionic acid. Increase in ammonia concentration resulted in significant shifts in microbial community structures. Syntorophobacter, Pelotomaculum, and Thermovigra were determined as the dominant groups of (potential) propionate oxidizing bacteria in the non-inhibitory condition, whereas Cryptanaerobacter and Aminobacterium were the dominant groups of (potential) propionate oxidizing bacteria in the ammonia-inhibitory condition. Methanoculleus and Methanosaeta were the dominant methanogens. Acetate-oxidation coupled with hydrogenotrophic methanogenesis might be enhanced with increases in the relative abundances of Methanoculleus and Tepidanaerobacter acetatoxydans under the ammonia-inhibitory condition. The results of the present study could be a valuable reference for microbial management of anaerobic digestion systems that are exposed to ammonia inhibition and propionic acid accumulation.

• 상하수도학회지
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• 제28권2호
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• pp.195-206
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• 2014

Immobilization of anaerobic ammonium oxidizing bacteria has been studied to enhance the biomass retention of the slowly growing bacteria and the process stability. The purpose of this study was to compare the nitrogen removal efficiency of granular and immobilized anammox bacteria with poly vinyl alcohol and alginate. The specific anammox activity of the granular, homoginized and immobilized anammox bacteria were $0.016{\pm}0.0002gN/gVSS/d$, $0.011{\pm}0.001gN/gVSS/d$ and $0.007{\pm}0.0005gN/gVSS/d$, respectively. Although the activity decreased to 43.7 % of the original one due to low pH and $O_2$ exposure during the homogination and the immobilization, it was rapidly recovered within 7 days in the following continuous culture. When synthetic T-N concentrations of 100, 200, 400, 800 mg/L were fed, the immobilized anammox bacteria showed higher nitrogen removal efficiencies at all operational conditions than those of granular anammox bacteria. When the sludge retention time was shorten below 30.7 days and the reject water was fed, the nitrite removal efficiency of the granular anammox bacteria dropped to 8 % of the initial value, while that of the immobilized anammox bacteria was maintained over 95 % of the initial one. The immobilization with poly vinyl alcohol and alginate would be a feasible method to improve the performance and stability of the anammox process.

• 한국축산식품학회지
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• 제28권5호
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• pp.675-680
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• 2008

The physical quality and microbial safety of Korean beef jerky was evaluated at various steps during its preparation. Microbial counts in raw beef demonstrated mesophillic bacteria at 4.20 Log CFU/g, psychrotrophic bacteria at 3.85 Log CFU/g, anaerobic bacteria at 4.90 Log CFU/g, and yeast and molds at 1.92 Log CFU/g. Spore-forming bacteria and coliforms were not detected in raw beef samples. Spices and spiced meats showed similar trends in microbial counts, demonstrating minimal microbial contamination during these stages of preparation. The final beef jerky product exhibited counts of mesophillic bacteria at 1.15-1.66 Log CFU/g, psychrotrophic bacteria at 1.15-1.66 Log CFU/g, and anaerobic bacteria at 0.81-1.72 Log CFU/g. Spore-forming bacteria, yeast and molds, and coliforms were not detected in beef jerky. Significant differences from added ingredients occurred for instron textural profile analysis traits for hardness. In general, Korean beef jerky with humectant and tenderizer had lower hardness than control (without humectant and tenderizer). Also, the sample added with 0.01% protease from Streptomyces griseus had lower hardness than all samples. All samples had 0.7l to 0.72 water activities, and the color and pH were not shown in significant changes of all samples.

• Applied Biological Chemistry
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• 제34권2호
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• pp.130-133
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• 1991

재래식 메주의 23개 시료에 대해서 내부층과 외부층의 부위별로 산생성균수 및 총균수를 조사하고 이들 균주를 동정하였다. 산생성균은 내부층에 비하여 외부층에 많았고 각 부위에 있어서 호기성 산생성균보다 혐기성 산생성균이 많은 경향을 나타내었다. 호기성 산생성균 중 비호염성균 및 내염성균의 평균균수는 각각 $24{\times}10^6$ 및 $33{\times}10^5cell/g$였고 혐기성 산생성균 중 비호염성균 및 내염성균의 평균균수는 $10{\times}10^7$ 및 $58{\times}10^5cell/g$였다. 호기성 산생성균 2균주는 Micrococcus sp., 혐기성 산생성균 3균주는 Strepococcus sp., Pediococcs sp. 및 Lactobacillus sp., 호기성 일반세균 2균주는 Bacillus sp.으로 각각 동정되었다.

• 한국임상수의학회지
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• 제17권2호
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• pp.459-463
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• 2000

A 3 year-old male American Pit Bull Terrier in Kwang-Ju, Chonnam was admitted to the Teaching Animal Hospital, Chonbuk National University. He showed convulsive spasms of skeletal muscles, raised tail-head, and rigidity of rigidity. Based on these clinical sign\ulcorner tetanus was suspected Hematological test results showed and WBC value (21 ,800/$\mu$l/) with neutrophils (17.877/$\mu$l) and monocytes (1.744/$\mu$l). The blood chemical values ALT (86 IU/L) and AST (119 IU/L) were elevated. Fecal sample from the dog was cultured in anaerobic chamber. A Gram positive, drumstick shaped bacteria was isolated. The bacteria was identified as Clostridium tetani by biological and biochemical tests. The dog was hospitalized in a quiet place where the light was cut off and treated with penicillin G procaine and phenobarbital sodium. The dog made a complete recovery 25 days after treatment. This Is the first report on C. tetani infection of domestic dog in Korea.

• 한방안이비인후피부과학회지
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• 제17권3호
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• pp.33-37
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• 2004

Objective: The aim of this work is to investigate the antibacterial activity of the Sohporaflavanone G isolated from Sophora flavescens (S. flavescens), as the development of microbial resistance to antibiotics make it essential to constantly look for new and active compounds effective against pathogenic bacteria. Method : Sophoraflavanone G was isolated from the dried roots of Sophora flavescens Aiton (Leguminosae) by bioassay?guided fractionation. We investigated the effect of sophoraflavanone G on oral bacterial at various concentrations after incubation of 24 h in strains in the dose?dependent manner. Results: The structure of active compound, Sophoraflavanone G having a lavandulyl group at C?8, was elucidated on the basis of spectral data especially 1H?NMR and I3C?NMR. The antimicrobial activity showed that Sophoraflavanone G exhibited antimicrobial activilies against all the bacteria tested (MICs, 0.39 - 6.25 ㎍／ml). Sophoraflavanone G showed the strong antimicrobial activity against all the facultative bacteria and microaerophilic bacteria (MICs, 0.78 - 1.56 ㎍／ml) and also Sophoraflavanone G showed the strong antimicrobial activity against obligate anaerobic bacteria (MICs, 0.39 - 6.25 ㎍／ml).