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http://dx.doi.org/10.5322/JESI.2021.30.12.1093

Effect of Ammonia Load on Microbial Communities in Mesophilic Anaerobic Digestion of Propionic Acid  

Trang, Le Thi Nhu (Division of Earth Environmental System Science (Major of Environmental Engineering), Pukyong National University)
Lee, Joonyeob (Department of Environmental Engineering, Pukyong National University)
Publication Information
Journal of Environmental Science International / v.30, no.12, 2021 , pp. 1093-1100 More about this Journal
Abstract
The present study investigated the effect of ammonia load on microbial communities in mesophilic anaerobic digestion of propionic acid. A laboratory-scale continuous anaerobic digester treating propionic acid as a sole organic substrate was operated under non-inhibitory condition and inhibitory conditions with ammonia (1.5 g and 3.5 g ammonia-N/L, respectively), and bacterial and archaeal communities in the steady states of each ammonia condition were analyzed using high-throughput sequencing. Thirteen bacterial families were detected as abundant bacterial groups in mesophilic anaerobic digestion of propionic acid. Increase in ammonia concentration resulted in significant shifts in microbial community structures. Syntorophobacter, Pelotomaculum, and Thermovigra were determined as the dominant groups of (potential) propionate oxidizing bacteria in the non-inhibitory condition, whereas Cryptanaerobacter and Aminobacterium were the dominant groups of (potential) propionate oxidizing bacteria in the ammonia-inhibitory condition. Methanoculleus and Methanosaeta were the dominant methanogens. Acetate-oxidation coupled with hydrogenotrophic methanogenesis might be enhanced with increases in the relative abundances of Methanoculleus and Tepidanaerobacter acetatoxydans under the ammonia-inhibitory condition. The results of the present study could be a valuable reference for microbial management of anaerobic digestion systems that are exposed to ammonia inhibition and propionic acid accumulation.
Keywords
Anaerobic digestion; Propionic acid; Ammonia; Microbial communities; Methanogens;
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