• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.257-264
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• 2019

Objective: Dairy cattle nutrient requirement systems acknowledge amino acid (AAs) requirements in aggregate as metabolizable protein (MP) and assume fixed efficiencies of MP used for milk protein. Regulation of mammary protein synthesis may be associated with AA input and milk protein output. The aim of this study was to evaluate the effect of nanoemulsified methionine and cysteine on the in-vitro expression of milk protein (casein) in bovine mammary epithelial cells (MAC-T cells). Methods: Methionine and cysteine were nonionized using Lipoid S 75 by high-speed homogenizer. The nanoemulsified AA particle size and polydispersity index were determined by dynamic light scattering correlation spectroscopy using a high-performance particle sizer instrument. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed to determine the cytotoxicity effect of AAs with and without nanoionization at various concentrations (100 to $500{\mu}g/mL$) in mammary epithelial cells. MAC-T cells were subjected to 100% of free AA and nanoemulsified AA concentration in Dulbecco's modified Eagle medium/nutrient mixture F-12 (DMEM/F12) for the analysis of milk protein (casein) expression by the quantitative reverse transcription polymerase chain reaction method. Results: The AA-treated cells showed that cell viability tended to decrease (80%) in proportion to the concentration before nanogenesis, but cell viability increased as much as 90% after nanogenesis. The analysis of the expression of genetic markers related to milk protein indicated that; ${\alpha}_{s2}$-casein increased 2-fold, ${\kappa}$-casein increased 5-fold, and the amount of unchanged ${\beta}$-casein expression was nearly doubled in the nanoemulsified methionine-treated group when compared with the free-nanoemulsified methionine-supplemented group. On the contrary, the non-emulsified cysteine-administered group showed higher expression of genetic markers related to milk protein ${\alpha}_{s2}$-casein, ${\kappa}$-casein, and ${\beta}$-casein, but all the genetic markers related to milk protein decreased significantly after nanoemulsification. Conclusion: Detailed knowledge of factors, such nanogenesis of methionine, associated with increasing cysteine and decreasing production of genetic markers related to milk protein (casein) will help guide future recommendations to producers for maximizing milk yield with a high level of milk protein casein.

• Asian-Australasian Journal of Animal Sciences
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• 제29권1호
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• pp.36-42
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• 2016

Milk-related traits (milk yield, fat and protein) have been crucial to selection of Holstein. It is essential to find the current selection trends of Holstein. Despite this, uncovering the current trends of selection have been ignored in previous studies. We suggest a new formula to detect the current selection trends based on single nucleotide polymorphisms (SNP). This suggestion is based on the best linear unbiased prediction (BLUP) and the Fisher's fundamental theorem of natural selection both of which are trait-dependent. Fisher's theorem links the additive genetic variance to the selection coefficient. For Holstein milk production traits, we estimated the additive genetic variance using SNP effect from BLUP and selection coefficients based on genetic variance to search highly selective SNPs. Through these processes, we identified significantly selective SNPs. The number of genes containing highly selective SNPs with p-value <0.01 (nearly top 1% SNPs) in all traits and p-value <0.001 (nearly top 0.1%) in any traits was 14. They are phosphodiesterase 4B (PDE4B), serine/threonine kinase 40 (STK40), collagen, type XI, alpha 1 (COL11A1), ephrin-A1 (EFNA1), netrin 4 (NTN4), neuron specific gene family member 1 (NSG1), estrogen receptor 1 (ESR1), neurexin 3 (NRXN3), spectrin, beta, non-erythrocytic 1 (SPTBN1), ADP-ribosylation factor interacting protein 1 (ARFIP1), mutL homolog 1 (MLH1), transmembrane channel-like 7 (TMC7), carboxypeptidase X, member 2 (CPXM2) and ADAM metallopeptidase domain 12 (ADAM12). These genes may be important for future artificial selection trends. Also, we found that the SNP effect predicted from BLUP was the key factor to determine the expected current selection coefficient of SNP. Under Hardy-Weinberg equilibrium of SNP markers in current generation, the selection coefficient is equivalent to $2^*SNP$ effect.

• Journal of Animal Science and Technology
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• 제55권1호
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• pp.1-6
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• 2013

본 연구는 국내 국가단위 평가결과의 문제점을 해결하면서 유전 능력평가시스템을 고도화하고 우리나라가 국제유전평가에 참여하기 위하여 국제평가기구에서 요구하는 검증작업을 통과하기 위하여 수행하였다. 본 연구에 이용된 자료는 농협중앙회 젖소개량부에서 수집한 분만일이 2001년부터 2009년까지의 검정성적으로 총 1,416,589개이며 산차는 5산으로 제한하였으며, 누적착유일은 75~305일로 제한하였고, 전체 혈통자료는 2,279,741개이며 부모를 갖는 개체는 535,409개이고 아비소는 2,467두로 구성되어 있는 기록들을 이용하였다. 유량, 유지방, 유단백에 대한 육종가는 신규로 개발한 다형질모형(Multiple traits model)을 이용하여 육종가를 구하였으며 기초작업은 SAS version 9.2와 R프로그램을 이용하였으며 유전모수를 추정하기 위하여 VCE 6.0을 이용하였다. 전반적으로 유전적 추세는 꾸준히 지속되어 오고 있으며 산차별 차이가 두드러지게 나타나지는 않았다. 유지방을 제외하고 유량과 유단백 형질의 추세가 잘 추정되었음을 알 수 있다. 유전평가분석결과 상위 1000두의 랭킹안에 최근의 생년을 갖는 딸소가 기존에 사용한 모수로 분석한 결과보다 딸소가 증가했음을 알 수 있다. 국제유전평가모형을 통해 새로 평가한 결과 씨수소상위 100두를 기존 평가모형에 의한 평가결과와 비교했을 때, 2006년생은 23두에서 28두로 2007년생은 12두에서 20두로 2008년생은 2두에서 8두로 증가하였다. 이는 최근의 딸소나 씨수소의 유전능력이 우수함에도 불구하고 상위에 랭크되지 못했던 결과가 새 모형의 적용으로 보완하여 새로운 평가분석에 적용할 수 있을 것으로 판단된다. 따라서 기존의 단형질 모형 대신 다형질 모형을 이용한 분석방법으로 평가를 실시하고 국제유전평가에도 다형질 모형을 이용한 육종가를 제시함으로써 다형질 선발의 정확도가 향상될 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권1호
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• pp.40-46
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• 2018

Objective: To examine the effects of Rhodobacter sphaeroides (R. sphaeroides) supplementation as a direct-fed microbial (DFM) on rumen fermentation in dairy cows and on coenzyme Q10 (CoQ10) transition into milk, an in vitro rumen simulation batch culture and an in vivo dairy cow experiment were conducted. Methods: The characteristics of in vitro ruminal fermentation were investigated using rumen fluids from six cannulated Holstein dairy cows at 2 h post-afternoon feeding. A control treatment was included in the experiments based on a typified total mixed ration (TMR) for lactating dairy cows, which was identical to the one used in the in vivo study, plus R. sphaeroides at 0.1%, 0.3%, and 0.5% TMR dry matter. The in vivo study employed six ruminally cannulated lactating Holstein cows randomly allotted to either the control TMR (C-TMR) treatment or to a diet supplemented with a 0.5% R. sphaeroides culture (S-TMR, dry matter basis) ad libitum. The presence of R. sphaeroides was verified using denaturing gradient gel electrophoresis (DGGE) applied to the bacterial samples obtained from the in vivo study. The concentration of CoQ10 in milk and in the supernatant from the in vitro study was determined using high performance liquid chromatography. Results: The results of the in vitro batch culture and DGGE showed that the concentration of CoQ10 significantly increased after 2 h of R. sphaeroides supplementation above 0.1%. When supplemented to the diet of lactating cows at the level of 0.5%, R. sphaeroides did not present any adverse effect on dry matter intake and milk yield. However, the concentration of CoQ10 in milk dramatically increased, with treated cows producing 70.9% more CoQ10 than control cows. Conclusion: The CoQ10 concentration in milk increased via the use of a novel DFM, and R. sphaeroides might be used for producing value-added milk and dairy products in the future.

• Journal of Animal Science and Technology
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• 제47권4호
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• pp.573-582
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• 2005

본 시험은 미역분말의 첨가가 홀스타인 젖소의 유생산과 내분비계에 미치는 영향을 규명하기 위하여 실시하였다. 기초사료에 미역분말을 1일 두당 800g 수준으로 급여하여 대조구와 비교하였다. 사료건물섭취량은 처리에 의한 영향을 받지 않았고 비유말기 산유량은 대조구에 비해 일일 6.25kg 이나 많게 유의하게 증가하였다(p<0.05). 하지만 유성분은 처리구간에 유의한 차이를 나타내지 않았다. 대조구의 체세포수는 유동적이었지만 처리구의 체세포수는 시험 종료 시 $10^5$개/ml로 시험개시에 비해 무려 53.77%의 감소효과를 보여 탁월한 체세포 감소효과를 나타내었다. 미역분말의 첨가가 혈중 면역글로부린의 함량에는 영향을 주지 않은 것으로 나타났다. 비유에 직접적인 영향을 미치는 IGF-1의 혈장농도는 미역처리구에서 유의성 있게 증가하였으며 비유와 직접적인 관련이 있는 또 다른 호르몬인 $T_3$ 와 $T_4$의 농도도 유의적으로 증가하는 양상을 나타내었다. 지질대사산물인 TC, HDL-C와 LDL-C의 농도는 모두 처리구에서 증가하는 양상을 나타냈다. 이와 같이 미역부산물의 사료 중 첨가는 유생산량의 증가를 보였는데 이는 혈중 비유관련 호르몬의 분비에 영향을 준 결과로 확인된 바 젖소사료자원으로서의 응용가치가 클 것으로 사료된다.

• 한국유기농업학회지
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• 제21권3호
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• pp.391-401
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• 2013

본 연구는 국내 유기축산물(시유) 생산농가에 대한 사양관리 실태 및 생산수준에 관한 조사 연구를 통해 애로사항을 파악하고 개선방안을 모색하고자 수행하였다. 2010년 말 현재 전체 낙농가 6,347호 중 유기축산물(시유) 인증 농가수는 35호로 0.57%였다. 유기축산물 인증농가 35호 중 11농가에 대한 조사결과, 호당 평균 총사육두수는 142두로 그 중 평균 착유우 69두, 건유우 13두, 육성우 및 송아지 60두였다. 성우 대비 후보축의 비율은 42.4%를 차지하고 있었다. 조사농가의 평균 산유량은 분포는 20kg 미만이 14.3%, 25~30kg 미만이 57.1%, 30kg 이상 28.6%로 분포되어 있었다. 조사농가의 유성분 및 유질분포를 보면, 유지율은 3.3%로 전국 평균 유지율 3.95%보다 많이 낮았다. 평균 세균수는 5,775개/ml로 1A 등급, 체세포수는 192,500개/ml로 1등급에 해당되어 유질등급은 우수한 것으로 나타났다. 유기축산물(시유) 생산이 농가소득에 도움이 되는지에 대한 조사결과, 도움이 된다는 응답이 90%로 나타났으나 실제적인 경영자료를 분석한 연구가 추가적으로 수행되어야 할 필요가 있다고 생각된다. 일반 우유 생산에서 유기축산물(시유)로 전환한 이유는 친환경적 목장경영을 위해서라고 응답한 비율이 54.5%로 가장 높았다. 일반 낙농에 비해 유기낙농의 어려운 점으로는 유기사료 공급의 어려움이라고 대답한 비율이 45.5%로 가장 높았다. 실제적으로 유기 조사료 자가 생산 이용률은 43.0%였고, 구입하여 이용하는 비율은 57.0%로 나타나 유기 조사료 자급에 대한 비율을 더 높이는 것이 유기낙농 발전을 위해 해결해야 할 과제임을 알 수 있다. 평균 조농비율은 38.6:61.4로 조사료 급여비율이 매우 낮음을 알 수 있었다. 향후 유기축산물(시유) 확대를 위해서는 유기사료의 안정적인 공급이 우선되어야 할 과제라 생각된다.

• 유통과학연구
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• 제14권11호
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• pp.83-90
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• 2016

Purpose - During the last two years, convenient stores (CS) are emerging as one of the most fast-growing retail trades in Korea. The goal of this work is to forecast and to analyze sales at CS using ARIMA-Intervention model (IM) and exponential smoothing method (ESM), together with sales at supermarkets in South Korea. Considering that two retail trades above are homogeneous and comparable in size and purchasing items on off-line distribution channel, individual behavior and characteristic can be detected and also relative superiority of future growth can be forecasted. In particular, the rapid growth of sales at CS is regarded as an everlasting external event, or step intervention, so that IM with season variation can be examined. At the same time, Winters ESM can be investigated as an alternative to seasonal ARIMA-IM, on the assumption that the underlying series shows exponentially decreasing weights over time. In case of sales at supermarkets, the marked intervention could not be found over the underlying periods, so that only Winters ESM is considered. Research Design, Data, and Methodology - The dataset of this research is obtained from Korean Statistical Information Service (1/2010~7/2016) and Survey of Service Trend of Korea Statistics Administration. This work is exploited time series analyses such as IM, ESM and model-fitting statistics by using TSPLOT, TSMODEL, EXSMOOTH, ARIMA and MODELFIT procedures in SPSS 23.0. Results - By applying seasonal ARIMA-Intervention model to sales at CS, the steep and persisting increase can be expected over the next one year. On the other hand, we expect the rate of sales growth of supermarkets to be lagging and tied up constantly in the next 2016 year. Conclusions - Based on 2017 one-year sales forecasts for CS and supermarkets, we can yield the useful information for the development of CS and also for all retail trades. Future study is needed to analyze sales of popular items individually such as tobacco, banana milk, soju and so on and to get segmented results. Furthermore, we can expand sales forecasts to other retail trades such as department stores, hypermarkets, non-store retailing, so that comprehensive diagnostics can be delivered in the future.

• 한국임상수의학회지
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• 제26권5호
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• pp.450-456
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• 2009

This study evaluated the efficacy of topical eprinomectin (0.5% pour-on solution, $Eprinex^{(R)}$, Merial) against Chorioptes texanus (C texanus) infestation in dairy cows reared in Korea. Dairy cows naturally infected with C texanus showed skin lesions as alopecia, erythema, excoriations and crusts on the tail head, the caudal folds, the back of udder and the rump area. Fourteen adult Holstein dairy cows with chorioptic mange at National Institute of Animal Science were selected on the basis of gross lesion and microscopic examination. Experimental dairy cows infected with chorioptic mange were treated with eprinomectin ($Eprinex^{(R)}$, Merial, France) at the rate of 1 ml /10 kg/BW. Evaluation of therapeutic effect is based on mite counts and gross lesion at weekly interval for 6 weeks. Milk yields were measured every day and calculate at the mean of milk yield at weekly interval. Mange mites absolutely removed after 1 week of topical eprinomectin against C. texanus infestation on dairy cows. The skin lesion of dairy cows infested with C. texanus recovered normal status after 6 weeks of eprinomectin treatment. After eprinomectin treatment, return to the normal level of milk yields in diary cows infested with C. texanus has indirectly indicated that C. texanus infestation have negative effect on productivity of dairy farm. However, it would be suggested that the investigation of actual condition and the effective control method of C. texanus infestation of dairy farms in Korea should be studied in the future.

• Journal of Animal Science and Technology
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• 제64권2호
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• pp.247-261
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• 2022

Ketosis is associated with high milk yield during lactating or insufficient feed intake in lactating dairy cows. However, few studies have been conducted on the metabolomics of ketosis in Korean lactating dairy cows. The present study aimed to investigate the serum and urine metabolites profiling of lactating dairy cows through proton nuclear magnetic resonance (¹H-NMR) spectroscopy and comparing those between healthy (CON) and subclinical ketosis (SCK) groups. Six lactating dairy cows were categorized into CON and SCK groups. All experimental Holstein cows were fed total mixed ration. Serum and urine samples were collected from the jugular vein of the neck and by hand sweeping the perineum, respectively. The metabolites in the serum and urine were determined using ¹H-NMR spectroscopy. Identification and quantification of metabolites was performed by Chenomx NMR Suite 8.4 software. Metabolites statistical analysis was performed by Metaboanalyst version 5.0 program. In the serum, the acetoacetate level was significantly (p < 0.05) higher in the SCK group than in the CON group, and whereas acetate, galactose and pyruvate levels tended to be higher. CON group had significantly (p < 0.05) higher levels of 5-aminolevulinate and betaine. Indole-3-acetate, theophylline, p-cresol, 3-hydroxymandelate, gentisate, N-acetylglucosamine, N-nitrosodimethylamine, xanthine and pyridoxine levels were significantly (p < 0.05) higher in the urine of the SCK group than that in the CON group, which had higher levels of homogentisate, ribose, gluconate, ethylene glycol, maltose, 3-methyl-2-oxovalerate and glycocholate. Some significantly (p < 0.05) different metabolites in the serum and urine were associated with ketosis diseases, inflammation, energy balance and body weight. This study will be contributed useful a future ketosis metabolomics studies in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.320-333
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• 2019

Objective: The Shanghai Holstein cattle breed is susceptible to severe mastitis and other diseases due to the hot weather and long-term humidity in Shanghai, which is the main distribution centre for providing Holstein semen to various farms throughout China. Our objective was to determine the genetic mechanisms influencing economically important traits, especially diseases that have huge impact on the yield and quality of milk as well as reproduction. Methods: In our study, we detected the structural variations of 1,092 Shanghai Holstein cows by using next-generation sequencing. We used the DELLY software to identify deletions and insertions, cn.MOPS to identify copy-number variants (CNVs). Furthermore, we annotated these structural variations using different bioinformatics tools, such as gene ontology, cattle quantitative trait locus (QTL) database and ingenuity pathway analysis (IPA). Results: The average number of high-quality reads was 3,046,279. After filtering, a total of 16,831 deletions, 12,735 insertions and 490 CNVs were identified. The annotation results showed that these mapped genes were significantly enriched for specific biological functions, such as disease and reproduction. In addition, the enrichment results based on the cattle QTL database showed that the number of variants related to milk and reproduction was higher than the number of variants related to other traits. IPA core analysis found that the structural variations were related to reproduction, lipid metabolism, and inflammation. According to the functional analysis, structural variations were important factors affecting the variation of different traits in Shanghai Holstein cattle. Our results provide meaningful information about structural variations, which may be useful in future assessments of the associations between variations and important phenotypes in Shanghai Holstein cattle. Conclusion: Structural variations identified in this study were extremely different from those of previous studies. Many structural variations were found to be associated with mastitis and reproductive system diseases; these results are in accordance with the characteristics of the environment that Shanghai Holstein cattle experience.