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http://dx.doi.org/10.5187/jast.2022.e14

Metabolic profiling of serum and urine in lactating dairy cows affected by subclinical ketosis using proton nuclear magnetic  

Eom, Jun Sik (Institute of Agriculture and Life Science, Gyeongsang National University)
Lee, Shin Ja (Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Hyun Sang (Institute of Agriculture and Life Science, Gyeongsang National University)
Choi, Youyoung (Division of Applied Life Science (BK21), Gyeongsang National Universitiy)
Jo, Seong Uk (Division of Applied Life Science (BK21), Gyeongsang National Universitiy)
Lee, Sang Suk (Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University)
Kim, Eun Tae (Dairy Science Division, National Institute of Animal Science, Rural Development Administration)
Lee, Sung Sill (Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Journal of Animal Science and Technology / v.64, no.2, 2022 , pp. 247-261 More about this Journal
Abstract
Ketosis is associated with high milk yield during lactating or insufficient feed intake in lactating dairy cows. However, few studies have been conducted on the metabolomics of ketosis in Korean lactating dairy cows. The present study aimed to investigate the serum and urine metabolites profiling of lactating dairy cows through proton nuclear magnetic resonance (1H-NMR) spectroscopy and comparing those between healthy (CON) and subclinical ketosis (SCK) groups. Six lactating dairy cows were categorized into CON and SCK groups. All experimental Holstein cows were fed total mixed ration. Serum and urine samples were collected from the jugular vein of the neck and by hand sweeping the perineum, respectively. The metabolites in the serum and urine were determined using 1H-NMR spectroscopy. Identification and quantification of metabolites was performed by Chenomx NMR Suite 8.4 software. Metabolites statistical analysis was performed by Metaboanalyst version 5.0 program. In the serum, the acetoacetate level was significantly (p < 0.05) higher in the SCK group than in the CON group, and whereas acetate, galactose and pyruvate levels tended to be higher. CON group had significantly (p < 0.05) higher levels of 5-aminolevulinate and betaine. Indole-3-acetate, theophylline, p-cresol, 3-hydroxymandelate, gentisate, N-acetylglucosamine, N-nitrosodimethylamine, xanthine and pyridoxine levels were significantly (p < 0.05) higher in the urine of the SCK group than that in the CON group, which had higher levels of homogentisate, ribose, gluconate, ethylene glycol, maltose, 3-methyl-2-oxovalerate and glycocholate. Some significantly (p < 0.05) different metabolites in the serum and urine were associated with ketosis diseases, inflammation, energy balance and body weight. This study will be contributed useful a future ketosis metabolomics studies in Korea.
Keywords
Metabolites; Subclinical ketosis; Lactating dairy cow; Serum; Urine; Proton nuclear magnetic resonance spectroscopy;
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