• BMB Reports
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• 제35권3호
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• pp.273-282
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• 2002

Sp3 is a bifunctional transcription factor that has been reported to stimulate or repress the transcription of numerous genes. Although the size of Sp3 mRNA is 4.0kb, the size of the known Sp3 cDNA sequence is 3.6kb. Thus, Sp3 functional studies have been performed with an artificially introduced start codon, and thus an amino-terminus that differs from the wild-type. Ideally, full-length cDNA expression vectors with the appropriate start codon should be utilized for these studies. Using 5'rapid amplification of cDNA ends, a full-length Sp3 cDNA clone was generated and the sequence verified in nine cell lines. No AUG initiation codon was present. However, stop codons were present in all three frames 5' to the known coding sequence. In vitro translation of this full-length cDNA clone produced the expected three isoforms-one at 100 kDa and two in the mid 60 kDa range. Electrophoretic mobility shift assays showed that the protein products had the ability to bind to the Sp1/3 consensus sequence. In vitro studies, using our Sp3 clone and site directed mutagenesis, identified the translation initiation site for the larger isoform as AUA. AUA has not been previously described as an endogenous initiation codon in eukaryotes.

• BMB Reports
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• 제44권11호
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• pp.725-729
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• 2011

We report a novel mechanism of a CDH1 splicing mutation in a patient with signet ring cell carcinoma of the stomach. A 27-year-old man complaining of aggravated dyspepsia was diagnosed with signet ring cell carcinoma. Both his father and uncle had died of stomach cancer at a young age. DNA sequencing analysis of the CDH1 gene revealed a splice site mutation (c.833-2A>G). By RNA/cDNA sequencing analysis, CDH1 c.833-2A>G generated a new acceptor site within intron 6, causing the insertion of a 79-bp intronic sequence between exon 6 and 7 (r.833-79_833-1ins), and resulting in a frame shift. E-cadherin immunohistochemical staining revealed a loss of CDH1 expression. This study reveals the disease-causing mechanism of this splicing mutation, and emphasizes the need for functional studies using RNA samples for the accurate interpretation of detected splicing variant. This is the first reported case of a CDH1 mutation in a Korean patient.

• 동의생리병리학회지
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• 제28권1호
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• pp.107-112
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• 2014

This study aimed to prove the effects of acupuncture treatment on acupoint Joksamni(ST36) related to gastrointestinal movement. Electrogastrogram (EGG) data were obtained in 11 healthy subjects treated by real or sham needle in single-blinded condition for 40 min, including before, during acupuncture treatment, and after removing needle. According to frequency analysis of EGG, median frequency(MF) was increased by real needle in 4 subjects, MF for another 2 subjects were increased by real and sham needle and no trend shift in MF showed in other 5 subjects. Our result showed that acupuncture treatment on Joksamni has some effects on gastrointestinal movement. In this study we have applied both real acupuncture and sham acupuncture on the same subjects. So its blinding was regarded as good but was effective in about 36%~55%. Two subjects were assumed that they showed placebo effects on both real and sham acupuncture. Therefore it is necessary to perform further study on case-control and a larger number of subjects with functional dyspepsia, in order to confirm our findings.

• 센서학회지
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• 제22권2호
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• pp.144-149
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• 2013

In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated $2{\mu}m$ of pore-depth by two-step anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.

• Archives of Pharmacal Research
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• 제27권5호
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• pp.524-530
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• 2004

Epilepsy or the occurrence of spontaneous recurrent epileptiform discharges (SREDs, seizures) is one of the most common neurological disorders. Shift in the balance of brain between excitatory and inhibitory functions due to different types of structural or functional alterations may cause epileptiform discharges. N-Methyl-D-aspartate (NMDA) receptor dysfunctions have been implicated in modulating seizure activities. Seizures and epilepsy are clearly dependent on elevated intracellular calcium concentration ([C $a^{2+}$]$_{i}$ ) by NMDA receptor activation and can be prevented by NMDA antagonists. This perturbed [C $a^{2+}$]$_{i}$ levels is forerunner of neuronal death. However, therapeutic tools of elevated [C $a^{2+}$]$_{i}$ level during status epilepticus (SE) and SREDs have not been discovered yet. Our previous study showed fast inhibition of ginseng total saponins and ginsenoside R $g_3$ on NMDA receptor-mediated [C $a^{2+}$]$_{i}$ in cultured hippocampal neurons. We, therefore, examined the direct modulation of ginseng on hippocampal neuronal culture model of epilepsy using fura-2-based digital $Ca^{2+}$ imaging and neuronal viability assays. We found that ginseng total saponins and ginsenoside R $g_3$ inhibited $Mg^{2+}$ free-induced increase of [C $a^{2+}$]$_{i}$ and spontaneous [C $a^{2+}$]$_{i}$ oscillations in cultured rat hippocampal neurons. These results suggest that ginseng may playa neuroprotective role in perturbed homeostasis of [C $a^{2+}$]$_{i}$ and neuronal cell death via the inhibition of NMDA receptor-induced SE or SREDs.d SE or SREDs..

• 한국산업정보학회논문지
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• 제12권5호
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• pp.110-119
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• 2007

최근 기업의 사업영역이 확대됨에 따라 다양한 이해관계자들과의 거래 및 협력관계가 이루어지고 있다. 이해관계자와의 효율적인 업무 프로세스 관리와 기업의 목표를 효과적으로 달성하기 위해 정보기술을 적극적으로 활용하고 있지만, 보안 등 정보기술의 위험성 또한 증가하고 있다. 이러한 시장 환경에 따라 IT 거버넌스 산업이 급속하게 성장하고 있으며, IT 거버넌스 솔루션을 제공하는 업체간의 경쟁 또한 심화되고 있다. 특히 IT를 기반으로 한 벤처기업의 시장진입이 이루어지고 있지만, 브랜드 관리를 포함한 마케팅 능력의 부재로 어려움을 겪고 있다. 본 연구는 스핀오프 벤처기업의 브랜드 명과 포지셔닝을 IT 거버넌스 솔루션을 개발한 기업의 사례를 중심으로 고찰해 보았다. 연구결과를 하면 첫째, IT 벤처기업은 브랜드이미지를 구축하는데 기업의 역량을 집중시킬 필요성이 있다는 것이다. 소비자에게 호감가고 독특하며 강한 브랜드 이미지를 구축해 놓으면 경쟁 제품의 공격으로 인한 범용품화의 위험을 극복할 수 있다. 둘째, 브랜드를 통해 소비자에게 제품의 기능적인 가치 이상을 창출하고 이를 전달해 나갈 수 있어야 한다. 즉 하이테크 기업도 소비자의 기능적 욕구에만 머물지 말고 고객의 감성적, 상징적인 욕구를 충족시켜 주는 것에도 관심을 가질 필요가 있다.

• 센서학회지
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• 제24권6호
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• pp.385-391
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• 2015

In this paper, we propose a new method to infiltrate $Fe_3O_4$-nanoparticles into a porous silicon film and a monitoring technique to detect packing density of nanoparticles within the film. Recently, research to use porous silicon as a drug carrier or a new functional sensor material by infiltrating $Fe_3O_4$-nanoparticles has been extensively performed. However, it is still necessary to enhance the packing density and to develop a monitoring technique to detect the packing density in real time. In this light, we forcibly injected a nanoparticle solution into a rugate-structured free-standing porous silicon (FPS) film by applying a pressure difference between the two sides of the film. We found that the packing density by the pressure-infiltration method proposed in this paper is enhanced, relative to that by the previous diffusion method. Moreover, a continuous shift in wavelength of the rugate reflectance peak measured from the film surface was observed while the nanoparticle solution was being injected. By exploiting this phenomenon, we could qualitatively monitor the packing density of $Fe_3O_4$-nanoparticles within the FPS film with the injection volume of the nanoparticle solution.

• 한국연극학
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• 제40호
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• pp.401-450
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• 2010

This thesis purposes to suggest that creative stage directors have copyright ownership and we make them create high-quality of theatre direction. Stage directors are sincere creator of the theatre stage today. We have little judicial precedents about stage directors, no artistic examination related directing. Stage directors are performers who have neighboring rights, there is a problem that they won't have the exclusive right of making derivative works in this country. Others will make creation (such as cinemas, animations, novels) based on stage expression without permission, because stage directors don't have exclusive right of making derivative works. Copyright law can't protect the concept of stage directors and building blocks of them which are drama text, actors and theatre space, because copyright law don't protect idea according to idea/expression dichotomy. The expression of stage direction is belong to five fundamentals which are composition, picturization, movement, rhythm, pantomimic dramatization that are come from Dean & Carra's work. Directors' work is to make theatrical works based on literary works. Therefore, theatrical works are derivative works which based on drama texts. Also, theatrical works are able to be joint works. In the case of that stage directors write drama text and create expression on the stage, they have to own authorship of both works. Merger doctrine should not apply theatre directors' works strictly like any other functional works because stage directors usually create noble expression which have been not before. We need shift of the definition of theatrical works which are derivative works or joint works to protect theatre directors' creativity. Hereafter, the special legal section for dramatic(theatrical) works including the flexible legal definition for performing arts should be established, and 'contract form' for stage directors should be made. Acting edition(literary works) should be published to grant creative directors compensation. I emphasize to grant ownership of copyright to creative stage directors, to encourage directors' works. Therefore, copyright law will be the support for development of cultural arts institutionally.

• 한국융합학회논문지
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• 제10권8호
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• pp.195-203
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• 2019

이 글은 인공지능과 인간이 함께 사회적 가치를 만들어 가는 작업이 중요하게 대두되는 현재의 시점에서 생활과 연관된 문화와 제도에 대한 패러다임의 전환을 모색해 보는 것을 목적으로 한다. AI와 관련된 현상들이 현대 사회에서 어떻게 작동하는가에 주목하는 접근 방법은 이 연구의 기초를 이룬다. 이에 "AI 현상"을 사회 문화의 일부로 수렴하면서 그 의미를 밝히기 위해 다양한 문헌 자료를 활용, 윤리나 기술평등 같은 가치를 연계시켜 AI의 사회 제도적 면을 짚어 보고자 하였다. 사회의 구성원들이 함께 할 수 있는 AI와 접목된 기술 문화를 추론하는 일도 기능적인 면에서의 기술적 이해 못지않게 중요한 일이다. 따라서 이 연구가 문화적 상상력과 사회적 시스템이 어우러져 만들어내는 새로운 문화, 즉 "인공지능 문화"의 가능성을 제시할 수 있기를 바란다. 그렇기에 이 글은 하나의 시론적인 성격도 더불어 갖는다.

• 동남아시아연구
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• 제28권2호
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• pp.119-145
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• 2018

2017년 창설 50주년을 맞은 아세안(ASEAN)은 제도적 보완과 기능적 협력을 강화하기 위해 일련의 선언과 행동 계획을 채택했다. 아세안의 '회합력'(convening power)이 아세안 중심성(centrality)과 회복력(resilience)을 촉진하고 있다. 반면 아세안 회원국의 민주주의 후퇴는 전통적 규범의 한계를 심화시키고 아세안의 이행력을 저하시킨다. 이 글은 아세안 주요 쟁점을 고찰하고 전망을 도출하고자 한다. 첫째, 아세안의 제도적 발전과 기능적 협력의 현황을 아세안 공동체 건설의 현황과 쟁점을 중심으로 살피고자 한다. 둘째, 아세안의 대외 관계를 중국, 미국, 한국과의 관계를 중심으로 살피고자 한다. 남중국해 이슈 및 중국의 경제적 영향력 확대는 아세안-중국관계의 복잡성을 심화시키고 있다. 트럼프 행정부의 대 동남아 정책 변화에 대응하기 위해서는 아세안의 단결이 필요하다. 한국의 신남방정책은 여러 제약에도 불구하고 양자 관계에 있어 전환점이 될 것으로 기대된다. 대외 환경의 변화 속에 아세안 단결과 중심성의 강화가 더욱 절실해지고 있다.