센서학회지 (Journal of Sensor Science and Technology)

  • 제22권2호
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  • Pages.144-149
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  • 2013
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  • 1225-5475(pISSN)
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  • 2093-7563(eISSN)
한국센서학회 (The Korean Sensors Society)
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폐암 바이오마커 검출용 나노SPR 바이오센서

Nano SPR Biosensor for Detecting Lung Cancer-Specific Biomarker

  • 장은윤 (경북대학교 센서 및 디스플레이공학과) ;
  • 염세혁 (경북대학교 기능성소자융합플랫폼센터) ;
  • 엄년식 ((주)유바이오메드) ;
  • 한정현 ((주)유바이오메드) ;
  • 김형경 ((주)유바이오메드) ;
  • 신용범 (한국생명공학연구원) ;
  • 강신원 (경북대학교 IT대학 전자공학부)
  • 투고 : 2013.02.01
  • 심사 : 2013.03.21
  • 발행 : 2013.03.31
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초록

In this research, we developed a biosensor to detect lung cancer-specific biomarker using Anodic Aluminum Oxide (AAO) chip based on interference and nano surface plasmon resonance (nanoSPR). The nano-porous AAO chip was fabricated $2{\mu}m$ of pore-depth by two-step anodizing method for surface uniformity. NanoSPR has sensitivity to the refractive index (RI) of the surrounding medium and also provides simple and label-free detection when specific antibodies are immobilized to the Au-deposited surface of nano-porous AAO chip. To detect the lung cancer-specific biomarker, antibodies were immobilized on the surface of the chip by Self Assembled Monolayer (SAM) method. Since then lung cancer-specific biomarker was applied atop the antibodies immobilized layer. The specific reaction of the antigen-antibody contributed to the change in the refractive index that cause shift of resonance spectrum in the interference pattern. The Limit of Detection (LOD) was 1 fg/ml by using our nano-porous AAO biosensor chip.

키워드

참고문헌

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