• Journal of the Korea Organic Resources Recycling Association
• /
• v.24 no.4
• /
• pp.85-94
• /
• 2016

The purpose of this study was to characterize the coffee ground and its possibility to develop the antifungal activity. pH, EC, and Zn contents of the coffee ground from coffee shops were higher than those of commercial coffee ground, but there was no significant difference in $K_2O$, CaO, MgO, $Na_2O$, Mn contents. The antimicrobial activity of the water soluble extracts from the coffee shop and the commercial coffee ground were tested for six major plant pathogens, Rhizoctonia solani, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora capsici, Alternaria alternata, and Botrytis cinerea. The result showed that there was reliable antifungal activity against all of tested plant pathogenic fungi. The inhibition effects of coffee ground compost extract on the spore germination and zoospore formation were investigated. Water soluble extracts of the coffee ground compost mixture added with 10% sesame oil cake were significantly inhibited the growth of conidia germination of A. altanata and zoospore formation of P. capsici in vitro. For investigating the functional materials of coffee ground compost, it was measured the total polyphenolic compounds contents with 30 days interval during decomposing coffee ground for 90 days. The total polyphenolic content increased with decomposing periods, and it observed that the highest total polyphenolic content was $0.35{\pm}0.03mg\;GAE/g$ on the 90th day in the coffee ground compost added with 10% sesame oil cake.

• Applied Biological Chemistry
• /
• v.18 no.4
• /
• pp.194-202
• /
• 1975

This study was conducted to study the properties of the water-soluble natural chelating agents from garbage compost and activated sewage sludge responsible for Fe chelation, which is closely associated with the effectiveness in correcting iron chlorosis in plant. The water-soluble fraction of these materials was fractionated by menas of Sephadex gel filtration and the fractions of Fe chehates were traced by radioactive $^{59}Fe$. The fractions were examined by ultraviolet and infrared. spectroscopy and stability constants for Fe. The water-soluble fraction from garbage compost was separated by Sephadex G-25 into approximately four fractions. Most of the added $^{59}Fe$ was associated with fraction I, which appeared at the void volume. Further fractionation by Sephadex G-50 indicated that the molecular weight of water-soluble chelating agents is in the approximate range of 5000 to 10,000. The water-soluble fraction from activated sewage sludge gave six fractions by Sephadex G-25. Most of the added $^{59}Fe$ was found in the fraction I,II, and III, The molecular weights of most chelating agents associated with $^{59}Fe$ appeared to be less than 5,000 and those of fraction I that appeared at the void volume was in the range of 5,000 to 1,000. Discrepancy between radio activity count and UV absorption indicated the heterogeneity of the fractions obtained by Sephadex gel filtration. Ultraviolet absorption spectra of all fractions separated by Sephadex G-25 and containing chelating agents showed no differences. Fraction IV and V of sewage extract showed absorption maxima and shifting similar to nucleic acid components suggesting the presence of decomposition products of nucleic acid. Similarity fraction VI contained phenolic type amino acid groups. Fraction I of compost extract contained most of the added $^{59}Fe$ and showed weak but extra definite absorption in the 1230, and $1270cm^{-1}$ region, suggesting that extra oxygen groups in polyphenolic structure were probably involved in Fe chelation. In sewage extract, fraction I,II, and III in which most of the $^{59}Fe$ was found, showed strong definite polypeptide absorption in the region of $1540cm^{-1}$ due to NH deformation and C-N stretching of amide groups in the peptidebond. These extra functional groups in fraction I, II, and III appeared to be associated with Fe chelation. The other fractions, not associated with $^{59}Fe$, still have carboxyl and hydroxyl groups, suggesting that these functional groups in these water extracts may not independently form the Fe chelates. Precipitation of ferric hydroxide precluded measuring the stability constants for Fe-chelates. However, the formation constants for Zn chelates as log K values for compost extract and sewage extract at pH 4.0 from which the strength of chelation with Fe could be presumed, were 8.23, and 9.75, respectively, indicating strong complexation with metals. The chelating capacity of compost extract containing 6.5 g organic matter per liter was 0.82 mM, and that of sewage extract containing 5.3 g per liter was 0. 64 mM.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.26 no.3
• /
• pp.23-31
• /
• 2018

This study was carried out to investigate the effects of livestock manure compost and fish-meal liquid fertilizer on the growth of cucumber and the soil properties for the stable production of organic cucumber. Cucumber was transplanted in greenhouse on the $6^{th}$ of April in 2017, and this experiment contained five treatments: livestock manure compost 100% (LC 100%), livestock manure compost 50% + fish-meal liquid fertilizer 50% (LC50 + LF50), livestock manure compost 50% (LC50), chemical fertilizer (NPK), and no fertilizer (NF). As a result, it was shown that soil chemical properties of LC50 + LF50 plot is not different from that of LC100 plot except for the EC content, but soil chemical properties of LC50 + LF50 plot is statistically significantly different from that of NPK plot except for pH. As a result of evaluating the functional diversity of soil microbial communities using Biolog system, the substrate richness (S) and the diversity index (H) were the highest in LC50 + LF50 plot. As a result of comparing the cucumber growth and yield, it was found that there was no statistically significant difference between the plant height and the fresh weight of LC100, LC50 + LF50, and NPK plot, but the plant height and the fresh weight of LC100, LC50 + LF50, and NPK plot were different from that of LC50 and NF plot. The yield of cucumber was the highest in NPK plot r(7,397 kg/10a), but there was no statistically significant difference in the yield of cucumber between NPK plot and LC100, LC50 + LF50 plot. The above-described results suggested that the livestock manure compost and fish meal liquid fertilizer can be used for organic cucumber production under greenhouse condition.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.26 no.4
• /
• pp.31-43
• /
• 2018

This study was conducted to investigate the effects of organic farmland soil and nutrient management on soil properties depending on organic (animal manure compost and green manure [hairy vetch]) and chemical fertilization, tillage and no-tillage, and crop rotation (corn-wheat, corn-.hairy vetch). It was found that the application of organic matter such as animal manure compost and hairy vetch, increased the soil organic matter content, the soil microbial density and microbial biomass C content as compared with the chemical fertilizer treatment. It was also confirmed that the functional diversity of soil microbial community was increased. As a result of the comparison with the crop rotation and single cropping, the soil chemistry showed no significant difference between the treatments, but the corn-wheat and corn-hairy vetch rotation treatments tended to have higher microbial biomass C content and shannon's diversity index than the single cropping. Soil chemical properties of tillage and no-tillage treatments showed no significant difference between treatments. There was no statistically significant difference in substrate utilization of soil microbial community between tillage and no-tillage treatment. Correlation analysis between soil chemical properties and soil microbial activity revealed that soil organic matter content and exchangeable potassium content were positively correlated, with statistical significance, with substrate utilization, and substrate richness. To conclude, organic fertilization had positive effects on the short-term improvement of soil chemical properties and diversity of microbial communities.

• The Journal of the Institute of Internet, Broadcasting and Communication
• /
• v.17 no.5
• /
• pp.173-179
• /
• 2017

Advanced countries, animal wastes are produced using bioenergy and methane gas technology. In Korea, many researches are being actively carried out to develop livestock manure as a resource technology rather than a animal waste. However, the production of bio-gas using livestock manure is still in the process of development of functional livestock and compost because of low economic efficiency with livestock manure recycling technology. In this paper, in order to accurately estimate the manure output, It will calculate the manure excretion if you have finished input the number of pigs. In addition, we simulated the fertilization rate of three elements of NPK fertilizer per 100 square meters automatically.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.8
• /
• pp.1197-1206
• /
• 2014

This study compared and analyzed the physiochemical and quality characteristics of young radish kimchi made with YR-FNC or YR-GC cultivated by organic farming as well as kimchi made with young radish cultivated by chemical composting/general farming (YR-Control). YR-Control showed higher contents of water and crude protein than YR-FNC and YR-GC kimchi but lower contents of crude ash, dietary fiber, and vitamin C. YR-FNC and YR-GC kimchi also showed higher contents of P and Cu than YR-Control but lower contents of N, K, Ca, Mg, Fe, and Zn. YR-Control fermentation progressed faster than that of YR-FNC or YR-GC after 24 days, and YR-Control more rapidly reached an appropriate pH. On the other hand, YR-FNC and YR-GC kimchi fermentation progressed slow and did not reach a pH level below 5.3. For period of fermentation, YR-Control and YR-FNC kimchi showed no significant difference in reducing sugar content, whereas that of YR-GC kimchi decreased significantly after 24 days of fermentation (P<0.002). YR-FNC and YR-GC kimchi showed 1.5~3 times higher total polyphenol and flavonoid contents than YR-Control at the beginning of fermentation, whereas YR-Control and YR-FNC kimchi showed a significant increase in total polyphenol and flavonoid contents during the fermentation period. By day 7 of fermentation, YR-FNC kimchi showed higher contents of lactic acid bacteria and total microbes than YR-Control. In the sensory evaluation, YR-FNC and YR-GC kimchi showed higher preference values than YR-Control. Therefore, kimchi made from young radish cultivated by organic farming has a longer freshness period than control kimchi and is characterized by excellent sensory quality, increased physiological contents, and improved beneficial health effects.

• KSBB Journal
• /
• v.25 no.3
• /
• pp.277-282
• /
• 2010

This study was performed to investigate the composition of amino acids and biological properties with the ethanol extract of fruiting bodies of Pleurotus eryngii grown on the sawdust compost mix (400 g sawdust plus 200 g rice bran) supplemented with various dosages of monosodium glutamate (MSG). Amino acid composition analyses showed that arginine, glutamic acid, alanine and glycine increased as the dosage of MSG was increased, whereas histidine, serine, methionine, isoleucine, leucine and phenylalanine did not increase. $\gamma$-Aminobutyric acid (GABA) content increased significantly up to 1.18 mg/g extract when 6 g MSG was supplemented to the sawdust mix. Antioxidant activity of the extract was estimated and compared to the standard antioxidant (ascorbic acid). The antioxidant property such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity increased with the extract samples of increasing dosage of MSG. Although the extract showed low levels of nitrite scavenging activity, this activity increased up to 1.5-2.0 fold when MSG was supplemented to the sawdust mix above a dosage of 0.5 g. The results obtained from the present investigation would appear that Pleurotus eryngii grown on the MSG-enriched sawdust mix can be used more effectively as one of potential sources of functional foods.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 2001.11a
• /
• pp.46-53
• /
• 2001

A commensal thermophile, Symbiobacterium toebii, isolated from hay compost (toebii) in Korea commensally interacted with a thermophilic Geobacillus toebii sp. nov., which was a new species within the genus Geobacillus on the basis of the phenotypic traits and molecular systematic data. S. toebii required the crude extracts and/or culture supernatant of the Geobacillus toebii for axenic growth and could grow on the temperature between 45 and $70^{\circ}C$ (optimum: $60^{\circ}C$; 2.4 h doubling time) and pH 6.0 and 9.0 (optimum: pH 7.5). The G+C content of the genomic DNA was $65 mol\%$, and the major quinones were MK-6 and MK-7. A phylogenetic analysis of its 16S rDNA sequence indicated that Symbiobacterium toebii was closely related with solely reported Symbiobacterium thermophilum. The presence of the commensal thermophile 16S rDNA and accumulation of indole in all the enriched cultures indicate that Symbiobacterium toebii is widely distributed in the various soils. The genome of S. toebii constituted a circular chromosome of 3,280,275 base pairs and there was not an extra-chromosomal element (ECE). It contained about 4,107 predicted coding sequences. Of these protein coding genes, about $45.6\%$ was encoded well-known proteins and annotated the functional assignment of 1,874 open reading frames (ORFs), and the rest predicted to have unknown functions. The genes encoding thermostable tyrosine phenol-lyase and tryptophan indole-lyase were cloned from the genomic DNA of S. toebii and the enzymatic production of L-tyrosine and L-tryptophan was carried out with two thermostable enzymes overexpressed in recombinant E. coli.

• Korean Journal of Soil Science and Fertilizer
• /
• v.42 no.4
• /
• pp.307-316
• /
• 2009

Soil microbial activity and diversity are affected by organic sources applied to improve soil quality and fluctuate seasonally. We investigated the effects of municipal compost (MC), poultry litter (PL), and cover crops of spring oats and red clover (RC) on soil enzyme activities, and soil bacterial community-level physiological profiling (CLPP) in a Mexico silt loam in North Central Missouri, USA. Temporal patterns of these parameters were observed by periodic five soil sampling from spring to fall over a two year period. MC increased soil dehydrogenase (DH) activity consistently beginning about three months after MC application; fluorescein diacetate (FDA) hydrolytic activity significantly began to increase by the September of the first year but fluctuated during the following period. DH activity responded more directly to the amount or properties of organic residues in soils while FDA hydrolysis and CLPP were generally influenced by composition of organic sources, and enzyme activities and CLPP showed seasonal variation, which depended on organic sources and soil moisture. MC and cover crops may be useful organic sources for enhancing general soil microbial activity and altering soil microbial diversity, respectively. Because microbial activities and diversity are dynamic and subject to seasonal changes, the effects of organic amendments on these parameters should be investigated frequently during a growing season.

• Korean Journal of Environmental Agriculture
• /
• v.21 no.3
• /
• pp.216-222
• /
• 2002

In order to evaluate the antifungal activity of bacteria against plant pathogenic fungi, 8 bacteria were isolated from mushroom compost hot spring, seaweed, and forest soil and mutants from them were induced by $LD_{95}$ gamma radiation($^{60}Co$). Bacillus circulans K1, Burkholderia gladioli K4 and Bacillus subtilis YS1 showed wide antifungal spectrum against 12 kinds of plant pathogenic fungi. From the radiation sensitivity test, B. gladioli K4 was very sensitive to gamma radiation and its $D_{10}$ value was 0.11 kGy. Antifungal activities of B. circulans Kl-1004 and B. subtilis YS1-1009, which were induced by the radiation of $^{60}Co$ increased against Botryosphaeria dothidea. The mutant strains, B. subtilis YS1-1006 and B. subtilis YS1-1009 were resistant to tebuconazole and copper hydroxide. SAR535, SAR5108, and SAR5118 mutated from Streptomyces sp. SAR01 were antifungal activity deficient mutants against 5 kinds of plant pathogenic fungi compared to wild strain, so that they could be supposed to be model strains far studying antifungal mechanism. It is suggested that various functional types of mutants could be induced by gamma radiation and applied usefully.