KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Characteristics and Biological Properties of Pleurotus eryngii grown on Monosodium Glutamate-enriched Media

글루탐산나트륨 첨가배지에서 재배된 새송이버섯의 특성 및 생리활성 연구

  • Yoon, Dong-Yeon (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Park, Ki-Moon (Department of Food Science and Biotechnology, Sungkyunkwan University) ;
  • Lee, Jae-Heung (School of Mechanical Engineering, Korea University of Technology and Education)
  • 윤동연 (성균관대학교 생명공학부) ;
  • 박기문 (성균관대학교 생명공학부) ;
  • 이재흥 (한국기술교육대학교 기계정보공학부)
  • Received : 2010.01.18
  • Accepted : 2010.06.24
  • Published : 2010.06.30
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Abstract

This study was performed to investigate the composition of amino acids and biological properties with the ethanol extract of fruiting bodies of Pleurotus eryngii grown on the sawdust compost mix (400 g sawdust plus 200 g rice bran) supplemented with various dosages of monosodium glutamate (MSG). Amino acid composition analyses showed that arginine, glutamic acid, alanine and glycine increased as the dosage of MSG was increased, whereas histidine, serine, methionine, isoleucine, leucine and phenylalanine did not increase. $\gamma$-Aminobutyric acid (GABA) content increased significantly up to 1.18 mg/g extract when 6 g MSG was supplemented to the sawdust mix. Antioxidant activity of the extract was estimated and compared to the standard antioxidant (ascorbic acid). The antioxidant property such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity increased with the extract samples of increasing dosage of MSG. Although the extract showed low levels of nitrite scavenging activity, this activity increased up to 1.5-2.0 fold when MSG was supplemented to the sawdust mix above a dosage of 0.5 g. The results obtained from the present investigation would appear that Pleurotus eryngii grown on the MSG-enriched sawdust mix can be used more effectively as one of potential sources of functional foods.

새송이 버섯 (Pleurotus eryngii) 재배배지에 글루탐산나트륨 (monosodium glutamate, MSG)을 혼합 첨가하여 재배한 자실체의 아미노산 및 $\gamma$-aminobutyric acid (GABA)함량 변화, 그리고 항산화 작용 등의 생리활성을 분석하여 새송이버섯의 기능성 향상 가능 여부를 조사하였다. 실험결과 MSG 첨가량이 증가함에 따라 arginine, glutamic acid, alanine, glycine 등 아미노산은 함량이 증가되었으며 histidine, serine, methionine, isoleucine, leucine, phenylalanine 등은 함량 변화는 거의 없었다. 한편 MSG 첨가량을 증가시킴에 따라 자실체의 GABA함량은 전체적으로 증가되었으며 MSG 6 g/bottle 첨가군에서 자실체내에 가장 높은 GABA함량 (1.18 mg/g)을 얻을 수 있었다. 또한 배지에 MSG첨가량을 증가시킴에 따라 자실체 추출물의 DPPH (1,1-diphenyl-2-picrylhydrazyl) radical 소거능 및 아질산염 소거능 (nitrite scavenging activity)도 증가됨을 알 수 있었다. 이러한 결과로부터 새송이버섯을 재배할 때 배지에 소량의 저렴한 MSG (~US $1/kg)를 첨가하면 아미노산 조성 및 항산화 작용 등 생리활성이 개선되는 고 기능성 새송이버섯 식품소재가 개발될 수 있을 것으로 사료된다.

Keywords

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