• 동의생리병리학회지
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• 제17권5호
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• pp.1224-1230
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• 2003

The purpose of this present study is to measure the changes of regional cerebral blood flow(rCBF) and blood pressure(BP) in rats. following the intravenous injection of drug. The measurement was continually monitored by laser-doppler flowmeter (Transonic Instrument, USA) and pressure tranducer(Grass, USA) in anesthetized adult Sprague-Dawley rats about for 2h to 2h and a half through the data acquisition system composed of MacLab and Macintosh computer. The result of this experiment was as following. 1. Fructus Schizandrae increased the changes of rCBF in rats, significantly. 2. The rCBF of Fructus Schizandrae did not change by pretreated propranolol. 3. The rCBF of Fructus Schizandrae did not change by pretreated atropine. 4. The rCBF of Fructus Schizandrae did not change by pretreated I-NNA. 5. The rCBF of Fructus Schizandrae was decreased by pretreated methylene blue. 6. The rCBF of Fructus Schizandrae did not change by pretreated indomethacin. 7. Fructus Schizandrae decreased the changes of blood pressure significantly. 8. The BP of Fructus Schizandrae did not change by pretreated propranolol. 9. The BP of Fructus Schizandrae did not change by pretreated atropine. 10. The BP of Fructus Schizandrae did nol change by pretreated I-NNA. 11. The BP of Fructus Schizandrae was decreased by pretreated methylene blue. 12. The BP of Fructus Schizandrae did not change by pretreated indomethacin. These results indicate that Fructus Schizandrae can increase the rCBF and decrease the BP, that related to guanylyl cyclase activity.

• 한방안이비인후피부과학회지
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• 제20권3호
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• pp.51-62
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• 2007

Objective : The aim of this study is to assess the effect of Schizandrae Fructus on melanin synthesis inhibition and whitening effect. Methods : We assessed inhibitory effects of Schizandrae Fructus on melanin-release from B16F10, on melanin production in B16F10, on mushroom tyrosinase activity in vitro, on tyrosinase activity in B16F10 and effect of Schizandrae Fructus on the expression tyrosinase, TRP-1, TRP-2, PKA, ERK-1 ERK-2, AKT-1, MITF in B16F10. Results and Conclusion : 1. Schizandrae Fructus inhibited melanin-release, melanin production in B16F10. 2. Schizandrae Fructus inhibited tyrosinase activity in vitro and in B16F10. 3. Schizandrae Fructus suppressed the expression of tyrosinase, TRP-1, TRP-2, PKA, ERK-2 in B16F10.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.294-299
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• 2003

The growth of Bacteroides fragilis was inhibited by combination of natural products bearing antioxidative capacity and combined two, three and four kinds of them. Bacteroides fragilis was controlled by Paeonia Japonica, Corni Fructus, Theae Folium, Coptidis Rhizoma alone, and two mixed combinations of Paeonia japonica and Scutellaria Baicalensis George, Scutellaria Baicalensis George and Schizandrae Fructus, Schizandrae Fructus and Theae Folium, Schizandrae Fructus and Corni Fructus, Schizandrae Fructus and Crataegi Fructus, Paeonia japonica and Schizandrae Fructus, and three mixed combinations of Paeonia Japonica and Scutellaria Baicalensis George and Schizandrae Fructus, Paeonia Japonica and Scutellaria Baicalensis George and Crataegi Fructus, Paeonia Japonica and Scutellaria Baicalensis George and Corni Fructus, and four mixed combinations of Scutellaria Baicalensis George and Crataegi Fructus and Corni Fructus and Schizandrae Fructus, Scutellaria Baicalensis George and Crataegi Fructus and Corni Fructus and Theae Folium. As these combinations of natural products will activate some parts of body, theymay be applied to pharmaceutical applications, functional foods, antiaging tea, alsoexpected to control bacterial growth for fermentative beverage bearing multifunction.

• Journal of Acupuncture Research
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• 제28권2호
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• pp.143-153
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• 2011

Objectives : The purpose of this study is to observe the effects of herbal acupuncture with Schizandrae Fructus solution at $GB_{34}$(Yangneungcheon) on hyperlipidemia in rats. Methods : In the present study, herbal acupuncture with Schizandrae Fructus solution was administered into hyperlipidemic mice. The author performed several experimental items to analyze the levels of various components and enzymes in serum, liver, as well as the histological changes of liver and aorta. Results : 1. In the Schizandrae Fructus herbal-acupuncture group, serum total cholesterol and Atherogenic index were significantly decreased, and the ratios of HDL to total cholesterol, and phospholipid to total cholesterol in serum were significantly increased as compared with those of the control group. 2. In the Schizandrae Fructus herbal-acupuncture group, HMG-CoA reductase activity was significantly decreased as compared with those of the control group. 3. Schizandrae Fructus herbal-acupuncture reduced deposit of lipid in the liver tissue. Conclusions : From the above results, it is suggested that Schizandrae Fructus herbal-acupuncture at $GB_{34}$ has a therapeutic effect on hyperlipidemia.

• 치위생과학회지
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• 제3권1호
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• pp.39-44
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• 2003

본 연구는 오미자, 오수유의 추출물의 S. mutans에 대한 항균효과를 paper disc method로 검색하여, S. mutans 생장에 관한 영향을 평가하였다 1. 오미자, 오수유 열수 추출물의 항균효과를 탐색한 결과 S. mutans에 대한 항균력이 우수한 순서는 오미자, 오수유 순이었으며, paper disc method에 의한 항균활성 측정(20 mg/ml 투여) 결과는 오미자가 8.5mm, 오수유가 6.5mm 이었다. 2. 한약재 추출물의 S. mutans 생장에 대한 효과는 대조군이 8시간에서 1.05로 최대 생장률을 보였으나, 오미자, 오수유 추출물을 2 mg/ml을 투여한 배지에서는 16시간만에 0.90, 0.80로 최대생장을 보였다. 3. S. mutans를 8시간 동안 배양한 후 배지내 pH를 측정한 결과 대조군은 5.08 이었으며, 오미자, 오수유 추출물이 투여된 배지는 6.00, 5.36 이었다. 4. S. mutans를 8시간 동안 배양한 후 배지내 탄수화물 변화량을 측정한 결과 대조군이 0.81 mg/ml 이었으며, 오미자, 오수유 추출물이 투여된 배지는 1.32 mg/ml, 1.88 mg/ml 이었다. 5. S. mutans를 8시간 동안 배양한 후 배지내 단백질 변화량을 측정한 결과 대조군은 8.39 mg/ml 이었으며, 오미자, 오수유 추출물이 투여된 배지는 8.59 mg/ml 9.97 mg/ml 이었다. 6. S. mutans의 다당류 생성에 미치는 효과는 대조군의 경우 배양 8시간에 300 mg/ml을 생성하였으나, 오미자, 오수유 추출물이 투여된 배지에서는 240 mg/100 ml, 280 mg/100 ml을 생성하였다.

• 대한간호학회지
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• 제31권2호
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• pp.268-278
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• 2001

Many women in the world have suffered from anemia produced by menstruation, pregnancy and delivery. In the theory of oriental medicine, a sour taste is believed to have a tonic effect on the blood. Thus this paper is to investigate the effect of sour tastes on the improvement in anemia-induced female mice, using citric acid and Fructus schizandrae(五味子). The method used in this experiment was the change of RBC, WBC, Hemoglobin, Hematocrit, Fe, and TIBC in the blood of female mice who were fed citric acid and Fructus schizandrae(五味子). The results obtained were as follows ; 1. Compared with the control group (anemia- induced group without treatment), the mean number of RBC in the blood of mice was significantly increased only in evaluating the change of sample group fed Fructus schizandrae (五味子) 500mg/kg. 2. Compared with the control group (anemia- induced group without treatment), the mean number of WBC in the blood of mice was not significantly larger in both sample groups fed citric acid and Fructus schizandrae(五味子). 3. Compared with the control group (anemia- induced group without treatment), the mean number of Hemoglobin in the blood of mice was significantly larger only in evaluating the change of the sample group fed Fructus schizandrae(五味子). 4. Compared with the control group (anemia- induced group without treatment), the mean percentage of Hematocrit in blood of mice was significantly increased only in evaluating the change of sample group fed Fructus schizandrae(五味子) 500mg/kg and sample group fed Fructus schizandrae(五味子) 250mg/kg for 3days. 5. Compared with control group (anemia- induced group without treatment), the mean volume of Fe in serum of mice was significantly increased only in evaluating the change of sample group fed Fructus schizandrae(五味子) 500mg/kg. 6. Compared with control group (anemia- induced group without treatment), the mean TIBC in serum of mice was significantly increased only in evaluating the change of sample group fed Fructus schizandrae(五味子) 500mg/kg and sample group fed Fructus schizandrae(五味子) 250mg/kg for 7days. According to these results, a sour taste is presumed to have a general tonic effect on anemia, but more study must be taken on the effects of citric acid in improving female anemia.

• 한국식품저장유통학회지
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• 제9권4호
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• pp.369-374
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• 2002

오미자 추출물에 감마선을 조사하여 tyrosinase 저해, xanthine oxidase 저해 및 아질산염 소거능에 대하여 검토하였다. 오미자 추출물은 열수, ethanol, methanol 그리고 acetone등을 이용하여 추출하였으며, 감마선은 10, 20과 30 kGy로 조사하였다. 오미자 추출물은 모두 tyrosinase 저해 효과를 가지고 있는 것으로 나타났다. 감마선 조사 후 오미자 추출물에 대한 tyrosinase는 열수 추출물보다 용매 추출물이 저해 효과가 높게 나타내었다. Xanthine oxidase 저해 효과실험 결과, 오미자 추출물은 열수 및 용매 추출물 모두 저해력을 나타내었으며, 감마선 조사는 xanthine oxidase 저해력에 영향을 미치지 않았다. 아질산염 소거능은 pH 1.2, 3.0 그리고 6.0으로 측정하였다. 오미자 추출물은 pH 1.2와 3.0 에서 높은 소거력을 나타내었고, 열수 추출물은 methanol, ethanol 그리고 acetone 추출물보다 아질산염 소거능이 훨씬 높게 나타내었다. 감마선을 30 kGy까지 조사하였을 때, 오미자 추출물의 생리 활성에는 크게 영향을 미치지 않았다.

• 생약학회지
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• 제19권3호
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• pp.193-200
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• 1988

Immunopotentiating activity of water extract of Lycii Fructus, Rubus Fructus, Torilis Fructus, Schizandrae Fructus and Cuscutae Semen were investigated using mouse fed with normal diet and protein deficient diet. Body weight, ratio of spleen or thymus to body weight, number of total peritoneal exudate cell, content of total serum protein and albumin content were decreased in the mouse fed with protein deficient diets as compared to the mouse fed with normal diet. Regardless of the nutritional condition, the supplement of the water extract of Lycii, Rubus Torilis, Schizandrae Fructus or Cuscutae Semen increased the ratio of spleen to body weight. The increment of this ratio was significant by the supplement of water extracts of Lycii and Torilis Fructus in mouse fed with protein deficient diet. The supplement of water extracts of Torilis Fructus to the mouse fed with protein deficient diet increased the content of total serum protein and albumin content significantly. Similar results were obtained with Lycii, Rubus, Schizandrae Fructus and Cuscutae Similar. The electrophorogram shows the difference in the concentration of some serum protein constituents between the mouse fed with normal diet and protein deficient diet.

• 한국식품영양과학회지
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• 제33권1호
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• pp.34-40
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• 2004

본 연구는 기능성 식품으로의 활용을 위한 한약자원에 관한 연구의 일환으로 계획되었고 선행연구와 문헌상의 기록을 바탕으로 한 오미자의 여러 기능 중 혈류개선효과에 중점을 두고 그 기능을 과학적으로 확인하여 보고자 하였다. 백서를 대상으로 LDF를 이용하여 오미자의 국소 뇌혈류량과 혈압에 대한 효능을 관찰한 결과는 다음과 같다. 오미자를 여러 농도로 투여시 국소 뇌혈류량은 증가되었다 오미자가 국소 뇌혈류량을 증가시키는 기전을 확인하기 위해 propranolol, atropine, L-NNA, indomethacin을 전처리한 후 오미자를 투여 했을 때는 국소 뇌혈류량에 유의한 변화를 관찰할 수 없었으나 methylene blue를 전처리한 후 오미자를 1.0 mg/kg 농도 이상으로 투여했을 때는 국소 뇌혈류량이 유의적으로 증가함을 관찰할 수 있었다. 오미자를 여러 농도로 투여시 혈압은 하강되었다. 오미자가 어떤 기전으로 혈압의 변화를 초래하는지를 확인하기 위해 propranolol, atropine, L-NNA, indomethacin을 전처리한 후 오미자를 투여했을 때는 혈압하강에 유의한 변화를 관찰할 수 없었으나 methylene blue를 전처리한 후 오미자를 1.0 mg/kg 농도이상으로 투여했을 때에는 혈압이 유의적으로 하강하는 변화가 나타났다. 이상의 결과에서 오미자는 뇌혈류량을 증가시키고 혈압을 강하시키는 작용이 있음을 확인하였고 그 기전은 guanylyl cyclase의 활성을 변화시킴으로써 나타나는 결과로 판단된다. 따라서 연구의 목적인 기능성 식품으로의 활용에 있어 원재료로 사용이 가능하다고 판단되며 향후 오미자를 이용한 기능성 식품의 제조 및 완제품에 대한 기능성을 평가하여 기능성 식품시장이 올바르게 정립되는데 기여하고자 한다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.195-200
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• 2003

The growth of S. aureus was inhibited by combination of natural products bearing antioxidative capacity and combined two, three and four kinds of them. S. aureus was controlled by Paeonia Japonica, Galla Rhois, Geranii Herba, alone, and two mixed combinations of Paeonia japonica and Scutellaria Baicalensis George, Paeonia japonica and Theae Folium, Paeonia japonica and Chelidonium Majus L, Paeonia japonica and Geranii Herba, Paeonia japonica and Schizandrae Fructus, and three mixed combinations of Paeonia Japonica and Scutellaria Baicalensis George and Geranii Herba, Paeonia Japonica and Scutellaria Baicalensis George and Chelidonium Majus L., Paeonia Japonica and Scutellaria Baicalensis George and Theae Folium, and four mixed combinations of Scutellaria Baicalensis George and Chelidonium Majus L. and Theae Folium and Schizandrae Fructus, Paeonia Japonica and Scutellaria Baicalensis George and Chelidonium Majus L. and Schizandrae Fructus, Scutellaria Baicalensis George and Geranii Herba and Theae Folium and Schizandrae Fructus. As these combinations of natural products will activate some parts of body, they may be applied to pharmaceutical applications, functional foods, antiaging tea, also expected to control bacterial growth for fermentative beverage bearing multifunction.