• 한국양식학회지
• /
• 제13권4호
• /
• pp.317-323
• /
• 2000

숭어 치어의 담수 사육을 위한 저염분 순화방법과 이에 따른 적응 능력을 높이기 위하여 염분사료를 공급하여 이에 따른 성장, 생존율 및 어체의 생리상태를 비염분사료구와 비교하였다. 실험 종료시 전장과 체중의 성장률은 해수사육구(SWN), 순차적으로 담수로 옮긴 염분사료구(GFWS) 및 순차적으로 담수로 옮긴 비염분사료구(GFWN)에서 빠르게 담수로 옮긴 염분사료구(FWS) 및 빠르게 담수로 옮긴 비염분사료구(FWN) 순서로 나타났다. 비만도는 모든 실험구에서 서로 차이를 보이지 않았다. 실험어의 생존율은 순차적으로 담수로 옮긴 염분사료구가 92.2${\pm}$2.2%로 가장 높았으나, 다른 실험구와 차이가 없었다. 어체의 수분함량은 빠르게 담수로 옮긴 비염분사료구에서 해수구보다 높게 나타나 차이를 보였다. 체액의 삼투질 농도는 실험 종료시에 모든 실험구에서 차이를 보이지 않았다. $Na^+$ 농도는 실험 종료시 해수사육구, 순차적으로 담수로 옮긴 비염분사료구, 순차적으로 담수로 옮긴 염분사료구 및 빠르게 담수로 옮긴 비염분사료구에서 차이를 보이지 않았다. 체액의 K/$^+$ 농도는 실험 종료시 해수사육구가 다른 실험구에 비교해 높은 수준을 나타냈다.

• Ocean and Polar Research
• /
• 제26권3호
• /
• pp.433-438
• /
• 2004

Stress response to the seawater acclimation in coho salmon (Oncorhynchus kisutch) smolt was investigated. Salt concentration of rearing water was gradually increased for 3 days from freshwater to seawater (30 ppt salt level). The changes of serum concentrations of cortisol as a primary stress indicator, and as secondary indicators, glucose (GLC), lactate (LAC), triglyce.ide (TG), cholesterol (CHOL), sodium ion $(Na^+)$, chloride ion $(Cl^-)$ and enzyme activities (alanine aminotransferase, ALT: aspartate aminotrasferase, AST; lactate dehydrogenase, LDH) were quantified during the acclimation experiment. Among them, cortisol, LAC, TG, CHOL, ALT, AST concentrations showed rapid increase at the first exposure to the 10ppt salt level (day 1), and began to decrease to the constant values after day 2 of adaptation at 20ppt salt level. However, LDH concentration tended to decrease during the whole experimental period. $Na^+\;and\;Cl^-$ showed slight decrease at day 1, and increased to a little bit higher values after day 2 rather than those in freshwater. All the fishes started on taking a food after day 4 of seawater adaptation. From these results, to reduce osmotic shock inducible stress to fish in seawater acclimation, gradual increase of salt levels is recommended.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
• /
• pp.216.2-216
• /
• 1997

No Abstract, See Full Text

• 한국양식학회:학술대회논문집
• /
• 한국양식학회 2006년도 수산관련학회 공동학술대회 발표요지집
• /
• pp.359-360
• /
• 2006

• 한국수산과학회지
• /
• 제44권3호
• /
• pp.225-231
• /
• 2011

Prolactin (PRL) plays an important role in freshwater (FW) osmoregulation by preventing the loss of ions and the uptake of water in fish. Growth hormone (GH) promotes acclimation to seawater (SW) in several teleosts. We acclimated rainbow trout Oncorhynchus mykiss weighting $68.2{\pm}16.6$, $138.3{\pm}24$, and $287.5{\pm}42.1$ g in separate experiments to SW under slow-acclimation (SSW) or acute-acclimation (ASW) conditions, and then examined the PRL and GH mRNA levels using the real-time quantitative polymerase chain reaction. The PRL mRNA levels in all three experimental groups decreased significantly with both the SSW and ASW treatments, as compared to a control group kept in FW for 30 days. The GH mRNA levels increased with ASW in the largest fish, whereas the levels in the other groups did not change significantly. The mortality rate of the largest fish was lower than for the other groups, whereas the growth rate among the three experimental groups did not differ significantly. The growth rate of the ASW group was highest for the smallest fish. These results suggest that SW acclimation is associated with the gene expression levels of PRL and GH in relatively large rainbow trout. In addition, the fish mortality and growth rate on FW-SW transfer seem to be related to body weight, and the SW acclimation method may be applied to the hatcheries industry.

• 한국수산과학회지
• /
• 제37권1호
• /
• pp.7-12
• /
• 2004

This study investigated the possibility of salinity acclimation of freshwater rotifers (Brachionus calyciflorus) as live food for sweetfish (Plecoglossus altivelis) larvae, and also examined the optimal salinity for the growth of sweetfish. Freshwater rotifers cultured in 0 and 4 PSU and seawater rotifers (B. rotundiformis) cultured in 33 PSU were supplied to the larvae with four kinds of enrichment material (condensed freshwater Chlorella, $\omega-yeast,$ baker's yeast, Super Selco) and larval growth at 4 PSU was examined. Growth of the freshwater rotifers positively increased from 0 PSU to 6 PSU, but decreased when over 8 PSU was reached. Growth and survival of the sweet fish larvae reared in 0 PSU were significantly lower than those reared in either 4 PSU or 33 PSU. This indicated that the freshwater rotifers (B. calyciflorus) could be used as live food for sweetfish larvae reared in 4 PSU. The body weight of sweetfish larvae fed on freshwater rotifers enriched with Super Selco was the highest at 0.163 mg, but there was no significant difference in survival and body length of the fish fed with the other enrichment materials. The content of n-3 HUFA of the sweetfish larvae fed on the freshwater rotifers enriched with Super Selco and the condensed freshwater Chlorella was higher than that enriched with $\omega-yeast$ and baker's yeast. These results indicated that B. calyciflorus cultured with the condensed freshwater Chlorella could be used for the sweetfish larvae without enrichment, and the most efficient enrichment material for B. calyciflorus is Super Selco.

• 수산해양교육연구
• /
• 제24권3호
• /
• pp.387-394
• /
• 2012

Fertilization rates were over 90% when sperm from black porgy Acanthopagrus schlegelii acclimated and raised in freshwater and black porgy raised in seawater were fertilized with eggs from female black porgy raised in seawater, although cryopreserved sperm were significantly lower in fertilization rates than non-cryopreserved sperm. From the observations of embryos development at different salinities (0, 10, 20 and 32 psu) within 3 hours upon fertilization (16 cell stage), all were dead at 0 psu. However, the development process and speed at 10 and 20 psu were the same as at 32 psu. But, many developing embryos were egg envelope injury at low-salinities of 10 and 20 psu unlike at 32 psu. This is attributable to osmotic shock. Hatching rate of embryos at 32 psu was 60% or so, whereas low as 5% or less were successfully hatched at 10 and 20 psu, implying that osmotic shock could be responsible for the failure of embryo development.

• Journal of Microbiology and Biotechnology
• /
• 제31권2호
• /
• pp.240-249
• /
• 2021

Phenotypic plasticity is a rapid response mechanism that enables organisms to acclimate and survive in changing environments. The Chinese mitten crab (Eriocheir sinensis) survives and thrives in different and even introduced habitats, thereby indicating its high phenotypic plasticity. However, the underpinnings of the high plasticity of E. sinensis have not been comprehensively investigated. In this study, we conducted an integrated gut microbiome and muscle metabolome analysis on E. sinensis collected from three different environments, namely, an artificial pond, Yangcheng Lake, and Yangtze River, to uncover the mechanism of its high phenotypic plasticity. Our study presents three divergent gut microbiotas and muscle metabolic profiles that corresponded to the three environments. The composition and diversity of the core gut microbiota (Proteobacteria, Bacteroidetes, Tenericutes, and Firmicutes) varied among the different environments while the metabolites associated with amino acids, fatty acids, and terpene compounds displayed significantly different concentration levels. The results revealed that the gut microbiome community and muscle metabolome were significantly affected by the habitat environments. Our findings indicate the high phenotypic plasticity in terms of gut microbiome and muscle metabolome of E. sinensis when it faces environmental changes, which would also facilitate its acclimation and adaptation to diverse and even introduced environments.

• 한국발생생물학회지:발생과생식
• /
• 제13권4호
• /
• pp.313-320
• /
• 2009

본 연구에서는 외인성 T3처리가 강도다리의 담수 순화시 삼투압 조절과 혈중 코티졸 변화에 어떠한 영향을 미치는지 조사하였다. 해수에서 사육 중인 강도다리에 갑상선호르몬의 일종인 T3를 5, 10 및 $15{\mu}g$/g body weight의 농도로 0.9% NaCl과 함께 주입하고 담수로 옮긴 후 3일째 혈액을 채취하였으며, 삼투압 조절 지표인 $Na^+,\;Cl^-$ 및 삼투질 농도와 스트레스 지표인 cortisol, glucose를 조사하였다. $10{\mu}g$/g의 T3를 주입한 실험구의 혈장 $Na^+$와 $Cl^-$의 농도는 호르몬 처리 없이 담수로 이동한 대조구에 비해 유의하게 높았다. 삼투질 농도는 $10{\mu}g$/g과 $15{\mu}g$/g의 농도를 주입한 실험구가 대조구보다 높았다. 그러나 $5{\mu}g$/g 농도의 T3를 주입한 실험구에서는 삼투압 조절에 영향을 미치지 않는 것으로 나타났다. 또한 본 연구에서는 T3를 주입한 모든 실험구에서 glucose와 상관없이 cortisol의 농도가 모두 증가하는 경향을 나타내었다. 이러한 cortisol의 증가는 스트레스 반응이 아닌 삼투압 조절과 관련이 있으며, 결과적으로 강도다리의 담수 순화시 외인 성 T3는 cortisol을 증가시킴으로 어체의 고삼투압 조절 능력을 향상시키는 것으로 추정된다.

• 한국양식학회지
• /
• 제22권1호
• /
• pp.28-33
• /
• 2009

본 연구에서는 강도다리의 담수순화방법으로 급격한 염분변화(AD)와 단계적인 염분변화(SD II과 III)를 주었을 때 강도다리의 생리적 반응을 조사하였다. AD에서 Ht, RBC 및 Hb는 Exp. II에 비해 증가하는 값이 높게 나왔으며 회복하는 속도도 느렸다. 그러나 SD II는 Ht, RBC 및 Hb에서 실험개시시수준과 실험종료시의 값이 유의한 차이를 보이지 않았다. AD에서 cortisol의 농도는 실험개시시 $2.1{\pm}1.0{\mu}g/mL$로부터 실험개시 후 6시간째에 $13.7{\pm}0.2{\mu}g/mL$ 급격히 증가하였으나 10일째에 $3.10{\mu}g/mL$로 실험개시시 수준으로 회복되었다. SD I에서 cortisol의 농도는 실험개시시 $2.1{\pm}1.0{\mu}g/mL$로부터 6시간째에 $13.6{\pm}0.6{\mu}g/mL$로 증가하였고, 실험종료시인 10일째에 $3.1{\pm}0.4{\mu}g/mL$로 회복되었다. SD II에서 cortisol의 농도는 실험개시시 $2.1{\pm}1.0{\mu}g/mL$로부터 6시간째와 12시간째에 각각 $10.5{\pm}2.5$, $10.8{\pm}5.6{\mu}g/mL$로 증가하는 경향을 보였으나 48시간째부터 실험개시시 수준으로 회복되는 경향을 보였다. 글루코스 농도는 AD, SD I과 II에서 cortisol의 경향과 다르게 유의한 차이를 나타내지 않았다. 삼투질농도는 실험개시시 $286.8{\pm}3.3\;mOsm/kg$으로 나타났으며, AD와 단계적 염분변화 실험구인 SD I, II에서는 실험종료 시에 각각 $266.3{\pm}8.5$, $242.0{\pm}12.5$, $269.5{\pm}4.9\;mOsm/kg$으로 유의하게 감소하는 경향을 보였다. 혈장의 $Na^+$, $K^+$ 및 $Cl^-$ 농도는 AD의 경우, $Cl^-$(mEq/L)만이 실험개시시의 수준과 유의한 변화를 볼 수 없었고, $Na^+$(mEq/L)와 $K^+$(mEq/L)는 유의하게 감소하는 경향을 보였다. SD I에서도 $Cl^-$(mEq/L)만이 실험개시시의 수준과 유의한 변화를 볼 수 없었고, $Na^+$(mEq/L)와 $K^+$(mEq/L)는 유의하게 감소하는 경향을 보였다.