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http://dx.doi.org/10.13000/JFMSE.2012.24.3.387

Fertilization Ability of Cryoperserved Sperms of Black Porgy Acanthopagrus schlegelii Acclimated in Freshwater  

Son, Maeng Hyun (National Fisheries Research and Development Institute)
Lim, Han Kyn (National Fisheries Research and Development Institute)
Do, Yong Hyun (National Fisheries Research and Development Institute)
Jeong, Min Hwan (National Fisheries Research and Development Institute)
Publication Information
Journal of Fisheries and Marine Sciences Education / v.24, no.3, 2012 , pp. 387-394 More about this Journal
Abstract
Fertilization rates were over 90% when sperm from black porgy Acanthopagrus schlegelii acclimated and raised in freshwater and black porgy raised in seawater were fertilized with eggs from female black porgy raised in seawater, although cryopreserved sperm were significantly lower in fertilization rates than non-cryopreserved sperm. From the observations of embryos development at different salinities (0, 10, 20 and 32 psu) within 3 hours upon fertilization (16 cell stage), all were dead at 0 psu. However, the development process and speed at 10 and 20 psu were the same as at 32 psu. But, many developing embryos were egg envelope injury at low-salinities of 10 and 20 psu unlike at 32 psu. This is attributable to osmotic shock. Hatching rate of embryos at 32 psu was 60% or so, whereas low as 5% or less were successfully hatched at 10 and 20 psu, implying that osmotic shock could be responsible for the failure of embryo development.
Keywords
Acanthopagrus schlegelii; Freshwater acclimation; Cryopreserved sperm; Fertilization; Salinity;
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