Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Serum Stress Responses during Seawater Acclimation in Coho Salmon, Oncorhynchus kisutch

은연어의 해수 순치에 따른 스트레스 반응의 변화

  • Hong, Kyung-Pyo (Marine Living Resources Research Division, KORDI) ;
  • Kim, Pyong-Kih (Department of Marine Bio-resources, Gangwon Provincial University) ;
  • Jeon, Joong-Kyun (Division of Marine Bioscience and Technology Kangnung National University) ;
  • Kim, You-Hee (Department of Marine Bio-resources, Gangwon Provincial University) ;
  • Park, Yong-Ju (Marine Living Resources Research Division, KORDI) ;
  • Myoung, Jung-Goo (Marine Living Resources Research Division, KORDI) ;
  • Kim, Jong-Man (Marine Living Resources Research Division, KORDI)
  • 홍경표 (한국해양연구원 해양생물자원연구본부) ;
  • 김병기 (강원도립대학 해양생물자원개발과) ;
  • 전중균 (강릉대학교 생명과학대학 해양생명공학부) ;
  • 김유희 (강원도립대학 해양생물자원개발과) ;
  • 박용주 (한국해양연구원 해양생물자원연구본부) ;
  • 명정구 (한국해양연구원 해양생물자원연구본부) ;
  • 김종만 (한국해양연구원 해양생물자원연구본부)
  • Published : 2004.09.30
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Abstract

Stress response to the seawater acclimation in coho salmon (Oncorhynchus kisutch) smolt was investigated. Salt concentration of rearing water was gradually increased for 3 days from freshwater to seawater (30 ppt salt level). The changes of serum concentrations of cortisol as a primary stress indicator, and as secondary indicators, glucose (GLC), lactate (LAC), triglyce.ide (TG), cholesterol (CHOL), sodium ion $(Na^+)$, chloride ion $(Cl^-)$ and enzyme activities (alanine aminotransferase, ALT: aspartate aminotrasferase, AST; lactate dehydrogenase, LDH) were quantified during the acclimation experiment. Among them, cortisol, LAC, TG, CHOL, ALT, AST concentrations showed rapid increase at the first exposure to the 10ppt salt level (day 1), and began to decrease to the constant values after day 2 of adaptation at 20ppt salt level. However, LDH concentration tended to decrease during the whole experimental period. $Na^+\;and\;Cl^-$ showed slight decrease at day 1, and increased to a little bit higher values after day 2 rather than those in freshwater. All the fishes started on taking a food after day 4 of seawater adaptation. From these results, to reduce osmotic shock inducible stress to fish in seawater acclimation, gradual increase of salt levels is recommended.

Keywords

References

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