Journal of Fisheries and Marine Sciences Education (수산해양교육연구)

The Korean Society for Fisheries and Marine Sciences Education (한국수산해양교육학회)
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Fertilization Ability of Cryoperserved Sperms of Black Porgy Acanthopagrus schlegelii Acclimated in Freshwater

담수순화 감성돔 Acanthopagrus schlegelii 동결보존 정자의 수정능력

  • Son, Maeng Hyun (National Fisheries Research and Development Institute) ;
  • Lim, Han Kyn (National Fisheries Research and Development Institute) ;
  • Do, Yong Hyun (National Fisheries Research and Development Institute) ;
  • Jeong, Min Hwan (National Fisheries Research and Development Institute)
  • Received : 2012.03.27
  • Accepted : 2012.06.03
  • Published : 2012.06.30
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Abstract

Fertilization rates were over 90% when sperm from black porgy Acanthopagrus schlegelii acclimated and raised in freshwater and black porgy raised in seawater were fertilized with eggs from female black porgy raised in seawater, although cryopreserved sperm were significantly lower in fertilization rates than non-cryopreserved sperm. From the observations of embryos development at different salinities (0, 10, 20 and 32 psu) within 3 hours upon fertilization (16 cell stage), all were dead at 0 psu. However, the development process and speed at 10 and 20 psu were the same as at 32 psu. But, many developing embryos were egg envelope injury at low-salinities of 10 and 20 psu unlike at 32 psu. This is attributable to osmotic shock. Hatching rate of embryos at 32 psu was 60% or so, whereas low as 5% or less were successfully hatched at 10 and 20 psu, implying that osmotic shock could be responsible for the failure of embryo development.

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References

  1. Alderdice, D.F.(1998). Osmotic and ionic regulation in teleost eggs and larvae. 99, 163-251. (in) Fish Physiology (eds) Hoar WS and Randall DJ. Vol. 11, Part A, Academic Press, New York.
  2. Go, H.B. & Rho, S.(1996). Low salinity tolerance of eggs and juveniles of tiger puffer, Takifugu rubripes, Journal of Aquaculture 9, 43-55.
  3. Guggino, W.B.(1980). Salt balance in embryos of Fundulus heteroclitus & F. bermudae adapted to seawater, American Journal of Physiology 238, 42-49.
  4. Gunnes, K.(1979). Survival and development of Atlantic salmon eggs and fry at three different temperatures, Aquaculture 16, 211-218. https://doi.org/10.1016/0044-8486(79)90109-1
  5. Hokanson, K.E.F., McCormick. J.M. & Jones, B.R.(1973). Temperature requirements for embryos and larvae of the northen pike, Esox lucius (Linnaeus), Transactions of the American Fisheries Society 102, 89-100. https://doi.org/10.1577/1548-8659(1973)102<89:TRFEAL>2.0.CO;2
  6. Holliday, F.G.T.(1965). Osmoregulation in marine teleost eggs and larvae, California Cooperative Oceanic Fisheries Investigations Reports 10, 89-95.
  7. Holliday, F.G.T.(1969). The effects of salinity on the eggs and larvae of teleost, 312-318, (in) Fish Physiology Vol. 1 (eds,) Hoar WS and Randall DJ, Academic Press, New York.
  8. Hwang, H.K., Kim, D.H., Park, M.W., Yoon, S.J. & Lee, Y.H.(2008). Effects of water temperature and salinity on the egg and larval of chub mackerel Scomber japonicus, Journal of Aquaculture 21, 234-238.
  9. Hwang, P.P. & Hirano, R.(1985). Effects of environmental salinity on intercellular organization and junctional stucture of chloride cells in early stages of teleost development, Journal of Experimental Zoology 236, 115-126. https://doi.org/10.1002/jez.1402360202
  10. Jeong, M.H., Lim, H.K., Kim, Y.S., Kim, S.Y. & Chang, Y.J.(2010). Sperm collection time, sex steroid hormones and gonadal development of black porgy, Acanthopagrus schlegeli acclimated in freshwater, Korean Journal of Fisheries Aquatic Science 43, 474-481. https://doi.org/10.5657/kfas.2010.43.5.474
  11. Jeong, M.H. & Chang, Y.J.(2011). Properties of Semen and Sperm Motility of Black Porgy Acanthopagrus schlegelii Acclimated in Freshwater, Development & Reproduction 15, 151-158.
  12. Lee, C.S., Tamaru, C.S., Kelley, C.D., Moriwake, A. & Miyamoto, G.T.(1992). The effect of salinity on the induction of spawning and fertilization in the striped mullet, Mugil cephalus, Aquaculture 101, 289-296.
  13. Lee, J.Y., Kim, W.K. & Chang, Y.J.(1997). Influence of water temperature and salinity in egg development of flatfish, Limanda herzensteini, Journal of Aquaculture 10, 357-362.
  14. Walsh, W.A., Swanson, C. & Lee, C.S.(1991). Combined effects of temperature and salinity on development and hatching of striped mullet, Mugil cephalus. Aquaculture 97, 281-289. https://doi.org/10.1016/0044-8486(91)90270-H
  15. Young, P.S. & Duenas, C.E.(1993). Salinity tolerance of fertilized egg and yolk-sac larvae of the rabbitfish, Siganus guttatus (Bloch). Aquaculture 112, 363-377. https://doi.org/10.1016/0044-8486(93)90396-G

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