Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Changes in Prolactin and Growth Hormone Gene Expression of Rainbow Trout Oncorhynchus mykiss Adapted to Seawater

해수순치에 따른 무지개송어 (Oncorhynchus mykiss)의 프로락틴 및 성장호르몬 유전자의 발현 변화

  • Shin, Ji-Hye (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Lee, Cheul-Ho (Yangyang Salmon Station, Korea Fisheries Resources Agency) ;
  • Jo, Mi-Hee (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University) ;
  • Hong, Kwan-Eui (Yangyang Salmon Station, Korea Fisheries Resources Agency) ;
  • Kim, Dong-Soo (Department of Marine Bio-Materials and Aquaculture, Pukyung National University) ;
  • Sohn, Young-Chang (Department of Marine Molecular Biotechnology, Gangneung-Wonju National University)
  • 신지혜 (강릉원주대학교 해양분자생명공학과) ;
  • 이철호 (수산자원사업단 양양연어사업소) ;
  • 조미희 (강릉원주대학교 해양분자생명공학과) ;
  • 홍관의 (수산자원사업단 양양연어사업소) ;
  • 김동수 (부경대학교 해양바이오신소재학과) ;
  • 손영창 (강릉원주대학교 해양분자생명공학과)
  • Received : 2010.11.23
  • Accepted : 2011.06.10
  • Published : 2011.06.30
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Abstract

Prolactin (PRL) plays an important role in freshwater (FW) osmoregulation by preventing the loss of ions and the uptake of water in fish. Growth hormone (GH) promotes acclimation to seawater (SW) in several teleosts. We acclimated rainbow trout Oncorhynchus mykiss weighting $68.2{\pm}16.6$, $138.3{\pm}24$, and $287.5{\pm}42.1$ g in separate experiments to SW under slow-acclimation (SSW) or acute-acclimation (ASW) conditions, and then examined the PRL and GH mRNA levels using the real-time quantitative polymerase chain reaction. The PRL mRNA levels in all three experimental groups decreased significantly with both the SSW and ASW treatments, as compared to a control group kept in FW for 30 days. The GH mRNA levels increased with ASW in the largest fish, whereas the levels in the other groups did not change significantly. The mortality rate of the largest fish was lower than for the other groups, whereas the growth rate among the three experimental groups did not differ significantly. The growth rate of the ASW group was highest for the smallest fish. These results suggest that SW acclimation is associated with the gene expression levels of PRL and GH in relatively large rainbow trout. In addition, the fish mortality and growth rate on FW-SW transfer seem to be related to body weight, and the SW acclimation method may be applied to the hatcheries industry.

Keywords

References

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