• Applied Biological Chemistry
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• 제35권5호
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• pp.367-374
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• 1992

이스라엘 잉어와 참잉어에 대한 농약의 독성 및 glycogen의 함량변화, 호소활성을 조사하고 농약 상호간의 협력작용의 여부를 조사하였다. $LC_{50}$치의 측정결과는 공시약제 중 endosulfan이 0.0079 ppm으로 가장 독성이 강했고 metalaxyl이 40 ppm 이상으로 가장 낮았다. 농약 상간의 협력작용은 IBP+isoprothiolane과 cartap+isoprothiolane 처리구에서 나타났으며 그 ratio(SR)는 각각 1.85, 1.53이었다. 효소활성의 경우 carboxylesterase와 glutathione S-transferase 모두 증가되었다. Esterase의 활성은 IBP 처리구에서 가장 높았고 isoprothiolane 처리구에서 제일 낮았으며, glutathione의 CDNB conjugation은 isoprothiolane 처리구에서 가장 높았고 isoprothiolane+cartap 처리구에서 가장 낮았다. LDH의 경우 isoprothiolane 처리구에서 활성이 가장 높았고 isoprothiolane+cartap 처리구에서는 가장 낮았다. Glycogen의 함량은 공시약제의 처리구 모두에서 감소를 보였으며 IBP 처리구에서 감소정도가 가장 높았다.

• 한국어병학회지
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• 제14권1호
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• pp.11-15
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• 2001

White Spot Baculovirus(WSBV) 미감염 새우 건강 종묘를 생산하기 위하여 난의 요오드 소독을 실시하였다. 유효 요오드농도 20,50,100,200 ppm에서 각 시험구의 대하(Penaeus chinensis) 수정난을 각각 30,60초 처리한 결과 각 농도의 30초 처리구에서는 50%이상의 상대부화율을 보였고, 60초 처리구에서는 20ppm 처리구에서만 약 50% 상대 부화율을 보인 반면 50 ppm이상에서는 부화율이 거의 없었다. 보리새우(Penaeus japoni cus)에서도 비슷한 결과를 보였다. 대하의 경우 세란만 실시한 대조구의 부화율은 33.3%이었지만, 보리새우는 55.2%로 상대적으로 높았다. 부화후 생존율을 보면 수정난의 소독으로 부화율은 낮아지지만 수정란 소독후 부화된 유생은 초기생육에는 문제점이 없었다. WSBV 미감염 건강종묘를 생산하기 위해서는 수정난을 세척하고 저농도의 요오드를 짧은 시간에 소독해야 한다.

• Parasites, Hosts and Diseases
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• 제23권1호
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• pp.33-40
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• 1985

1984년 11월 Echinostoma hortense에 의한 인체감염의 2례를 발견하였다. 이는 praziquantel치료와 두 환자가 함께 보획하여 생식한 바 있는 담수어의 추적조사로 확인되 었다. 또한 버들치(Moroco oxycephalus)가 E. hortense의 제이중간숙주 역할을 한다는 사실을 새로이 밝혔으며, 그 결과를 요약하면 다음과 같다. 1. 환자는 31세 및 30세 남자이며 강원도 원주시에 살고 있었고 한 가구에 같이 거주하고 있었다. 제1례는 E.H. fever의 병원에 입원하고 있었으며, 제2례는 건강한 상태로서 간헐적으로 가벼운 하복통과 연변 또는 경한 설사가 계속되었다. 대변검사에서 발견된 충란의 크기는 제1례가 $114.3{\times}71.0(평균){\mu}m$, 제2례가 $119. 1{\times}68.3(평균){\mu}m$이었다. 환자들에게 praziquantel 10~15mg/kg을 단회투여 하고 하제를 사용한 비 제2례의 환자 설사변에서 6마리의 E. hortense 성충을 수집하였다. 2. 환자들이 담수어를 보획한 그 장소에서 버들치 84마리와 붕어 20마리를 잡아서 피낭유충을 조사한 결과 버들치 3마리(3.5%)서 Echinostoma sp.의 피낭 유충을 발견하였다. 3. 분리된 피낭유충 3한마리의 흰쥐에게 경구 감염 시켜서 3마리의 E. hortense 성충을 얻는데 성공하였다. 이상의 결과로 충란만 발견된 제1례의 환자로 E. h hortense 감염자라는 것이 확인되었으며 버들치가 E. hortense의 제이중간숙주 역할을 한다는 사실이 밝혀졌다.

• 한국식품영양과학회지
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• 제28권5호
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• pp.1099-1106
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• 1999

Effects of dietary carotenoids were investigated on metabolism of the carotenoids, and body pigmen tation in oily bittering, Acheilognathus koreensis. Two weeks later after depletion,oily bitterings were fed the diets supplemented with either lutein, cynthiaxanthin and astaxathin for 4 weeks. Carotenoids distributed to and metabolized in integument were analyed. The carotenoid isolated from the integument of wild oily bittering, composed of 47.2% zeaxanthin, 11.4% lutein epoxide, 11.0% diatoxanthin, 9.7% lutein and 8.3% zeaxanthin epoxide. Meanwhile, two weeks later after depletion, the carotenoid composed of 29.9% crytoxanthin, 19.3% zeaxanthin, 13.2% lutein epoxide, 12.0% diatoxanthin and 8.8% zeaxanthin epoxide. These indicated that zeaxanthin, diatoxanthin, lutein epoxide and zeaxanthin epoxide were actively metabolized in oily bittering, compared to that of other fresh water fish. Total carotenoid content in the integument of wild oily bittering and oily bittering depleted for two weeks was found to be 1.72mg% and 2.08mg%, respectively. Two weeks later after treatment of experimental diet, total carotenoids content was increased to 2.23mg% in lutein, 2.36mg% in cynthiaxanthin and 2.49mg% in astaxanthin supplemented group, which were relatively higher than 2.10mg% in control group. Meanwhile, 4 weeks later, total ca rotenoids content was decreased to 1.76mg% in control, 1.95mg% in lutein, 1.74mg% in cynthiaxanthin and 1.72mg% in astaxanthin supplemented groups. These result indicate that dietary carotenoids were rapidly accumulated and then metabolized to certain metabolites shortly after feeding. Body pigmentation effects of the carotenoids due to accumulation of carotenoids in the integument of oily bittering was the most effectively shown in the astaxanthin supplemented group, followed by cynthiaxanthin and lutein supplemented groups. In the integument of oily bittering, dietary carotenoids were presumably biotrans formed via either oxidative or reductive pathways as presumed the variation of total carotenoid content and carotenoid composition in all experimental groups. The lutein was oxidized either to astaxanthin via doradexanthin and doradexanthin, or to zeaxanthin epoxide via zeaxanthin by oxidative pathway. Cynthiaxanthin was converted either to diatoxanthin and zeaxanthin by reductive pathway and then to zeaxanthin epoxide by oxidative pathway, or it was converted to astaxanthin via diatoxanthin, zeaxan thin and doradexanthin by oxidative pathway. Astaxanthin was converted to doradexanthin and zeaxanthin by reductive pathway and then to zeaxanthin epoxide by oxidative pathway. These results suggest that, oxidative pathway of carotenoids was major metabolic pathway along with reductive path way in fresh water fish.

• 한국농공학회지
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• 제24권1호
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• pp.44-52
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• 1982

Recently, estuary reserovoirs have been actively constructed in Korea and also in Japan there are a large number of estuary reservoirs constructed. But most of the estuary reservoirs are located at the downstream of a river where geographical condition is best for the construction of an enclosing dam. And an effective utilization of water from the estuary reservoir seems to be difficult even if estuary reservoirs are considered to be the water resources the most available for their watershed. Studies on estuary reservoirs so far have been mainly concentrated on the physical and engineering problems of the dam construction itself. The purpose of the present study is to review the estuary reservoir planning in connection with the water resources development and to study a basis of the planning. First, the levels of water use in Korea and Japan were compared with those of other countries in the world. And then, some representative reservoirs were selected to study the roles of a reservoir and water-using conditions in the watershed. Based on the study, a survey was given on the relation between a dam construction upstream and an estuary reservoir construction downstream of a river. Finally, a comprehensive examination was made of the bases of estuary reservoir planning. (1) The estuary reservoir planning is deeply related to the plan for water use develo- pment in the watershed. After the upstream water resources were fully developed up to the most, water reso- urces development by an estuary reservoir should be started. (2) If an estuary lake has a capacity big enough, it can store flood discharge of the watershed without any loss and become a basic facility that will bring about the maxi- mum use of water from the watershed. (3) Estuary reservoirs store water used in the upstream watershed, so recycling of water use is attained by the reservoir. Water in the estuary lake is difficult to be fresh water in its long run. Therefore, estuary reservoir should be located at a place where polluted water is purified and refused. All the planning should be based on the assumption that water in the estuary lake is not fresh but polluted after a long time. (4) The estuary lake can only supply water to the lower basin directly. But the upstream area is benefited from the estuary lake by exchange of irrigation water sources between the lower and the upper area. So a large-scale exchange plan between new and existing water resources is important. By constructing estuary reservoirs and the exchange of water sources between upper and lower areas, the reasonable maximum use of water from the whole watershed is at- tained. (5) The big problem coming from the water resources development by an enclosing estuary is salt water intrusion into the lake. To maintain the estuary lake salt-free, multi-purpose use of the lake should be avoided. It is necessary to take such fundamental measures as abolition of back flow operation of gate, and the closing of the fish port and the fish ladder. The results mentioned above were found in this study and these results of this study could be used for the adequate planning of estuary reservoirs in connection with the maximum water use of the watershed.

• 한국식품영양과학회지
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• 제25권5호
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• pp.871-877
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• 1996

담수산 어류의 고도이용과 이들 추출물의 기능적 특성 해명을 위하여 전통적인 건강보양식품으로 애용되어 온 담수산 어류를 대상으로 열수추출물과 효소 가수분해물을 제조하고 이들의 ACE 저해작용 및 gel 여과에 의한 ACE 저해획분을 분리하고 그 특성을 알아 보았다. 열수추출물과 효소 가수분해물의 ACE 저해활성은 담수어류 중 잉어가 가장 높았으며, 효소가 수분해물이 열수추출물에 비하여 강한 경향을 보였다. Ethanol가용성 획분이 침전획분에 비하여 강한 ACE 저해능을 나타내었으며, 잉어 효소 가수분해물의 70% ethanol 가용성 획분이 가장 높은 ACE 저해율을 나타내었다. ACE 저해효과를 지니는 활성획분의 분자량은 열수추출물에서는 약 1,400이었으며 효소 가수분해물에서는 1,400 보다 다소 큰 것으로 나타났고, 시료 중 잉어 효소 가수분해물이 가장 높은 ACE 저해활성을 나타내었다. 활성회분의 아미노산 조성은 열수추출물에 있어서는 glycine, alanine, leucine, proline등의 합량이 많았으며 효소 가수분해물에 있어서는 aspartic acid, glutamic acid, glycine, alanine, valine, leucine, proline 등의 함량이 많았다. 활성획분의 $IC_{50}$/은 효소가수분해물이 열수추출물에 비하여 낮은 경향이었다.

• Parasites, Hosts and Diseases
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• 제29권4호
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• pp.325-338
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• 1991

경북 영양지역 주민들의 흡충류 감염실태와 제2중간숙주 담수어의 흡충류 피낭유충의 기생상을 규명하기 위해 역학조사를 시행하였다. 충란검사는 반정량적 formalin-ether 집란법에 의거하였고 흡충류 충란양성자는 Stoll씨 충란계산법으로 감염정도를 추정하였다. 흡충류 피낭유충의 기생상은 슬라이드 글라스 여박법과 인공소화법으로 조사하였다. 피검자 955명중 18.5%(177명)에서 요충군이 검출되었고 그 중 흡충류 충란은 간흡충 단독감염자 7.3%(70명), 이형흡충류 단독감염자 7.7%(74명), 간흡충과 이형흡충류 이중감염자 2.5%(24명), 간흡충과 경충 이중감형자 0.1%(15명), 및 간질 위감염자 0.1%(1명)으로 17.9%(171명)에서 검출할 수 있었다. 성별 간흡충 감염은 남성 675명중 12.6%(85명), 여성 280명중 3.6%(10명)으로 남성에서 유의적으로 높았다. 이형흡광류 감염 역시 남성 675명중 12.0%(81명), 여성 380명중 6.1%(17명)으로 남성에서 유의적으로 높았다. 감염정도도 우흡충 모두 남성에서 여성보다 높았다. 연령별로는 30세 이상군에서 30세 미만군보다 심한 감염정도를 나타내었다. 10∼19세군에서는 양 흡충류의 감염자가 없었다. 감염정도와 연령사이에는 유의한 연관을 인정할 수 없었다. 반변천에서 채집된 8종의 담수어중 횡천흡충 피습유충의 기생률과 고 정도는 각각 갈겨니에서 57.5%, 12.4개로 가장 높았고 동사리에서 6.8%, 0.2개로 가장 낮았으며 피래미에서 55.4%, 1.3개로 중간을 나타내었다. 간흡충 피낭유충은 몰개에서만 기생률 23.3%, 그 기생정도 2.1개로 나타났다. 이상의 성적으로 미루어 보아 경북 영양지역은 간흡충과 이형흡충류의 만연지임을 알 수 있었다.

• 한국수산과학회지
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• 제37권2호
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• pp.116-121
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• 2004

Physiological responses and tolerance limit of hybrid striped bass $(Morone\;Chrysops\;male{\times}\;M.\;saxatilis\;female),$ were investigated on salinity changes from 33 psu to 0 psu and from 0 to psu 33 psu in two separate trials, respectively. In the acute salinity decrease from sea water (33 psu) to fresh water (0 psu), plasma level of cortisol, glucose, total protein, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not affected, however, $Na^+,\;Cl^-$ and osmolality significantly decreased. In the acute salinity increase from fresh water to sea water (33 psu), plasma cortisol level did not change. Glucose and total protein concentrations in plasma and hematocrit (Hct) decreased, whereas AST, ALT, $Na^+,\;Cl^-$ and osmolality increased. The hybrid striped bass can overcome the acute changes of salinity, from 0 psu to 33 psu or from 33 psu to 0 psu, But salinity decrease stressed the fish much more than salinity increase.

• 한국조리학회지
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• 제2권
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• pp.169-181
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• 1996

Today we are inclined to dine out more frequently according as the national income is increased and Women enter into the Social life, 'This tendency results in the universality of foodculture and the food made of fresh food stuff as well as the Pollution-free food is preferable as everyone warits to eat natural food or health food and escapes from the pollution. Moreover we have socail problems like fatress and adult disease which result from the small amount of activity or the food with high calory and high gat. under this circumstance. the most adeguate food seems to be Italian good. It stand in the spottight of food world and is most preferable for everyone today. The sphere of food and beverage has a low value-added-rate compartry to the high increasing rate of customers and profit, so, the efficient managing method for the cost of food and beverage and the operating skill in the kitchen are the principal factors leading to success or failure. Therefore, this research is to show some remedies as follows, pointing out the operating problems of an selected Italian restaurant at the top class hotel. First, most Italian restaurant at the hotel are located in the best place and investing the big capital, But they are open for only 8 hours through a day. This should be improved not to bring a enormous loss to the hotel The brealefast menus such as vegetables or fish food, pizza and pasta which modern man can easily enjoy should be developed open the restaurants for morning gours. Second, the business hours are not made continously. As a result, all the emplyees should stay in the company all day long without taking any advantage of the losed time, This will be the loss for both the company and the employees, By veloping the morning menus, employees shald be classified into morning and afternoon staffs and the opening and closing hour at 3 and 6 O'clock should be used so that minimum number of employees may operate the company. Third, the characteristic of Italian food is in the use of fresh food stuff, The proper purchase of food stuff and the simple distributing system will help Italian restaurant show its character istcc as a hzgh-grade restaurant. Fourth, the most popular Italian food such as pizza or pasta should be market with Priority.

• Animal cells and systems
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• 제16권2호
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• pp.162-171
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• 2012

We investigated food webs of a $Salix$ $subfragilis$-dominated wetland in the Janghang wetland in the Hangang estuary, which is very close to the Demilitarized Zone, along the west coast of Korea. Our study focused on understanding sesarmine crab ($Sesarma$ $dehaani$)-related food webs in a $S.$ $subfragilis$ forest. For our study, we used carbon and nitrogen stable isotopes and fatty acid biomarkers. We collected samples of plants, animals, and detrital sediment from four quadrats ($5{\times}5m^2$) set in the $S.$ $subfragilis$ community. Samples were collected from September 2006 to June 2009, except during the winter hibernation period of $S.$ $dehaani$. In the wet season, the sediment showed relatively high ${\delta}^{13}C$ and low ${\delta}^{15}N$ signatures compared with relatively low ${\delta}13C$ and high ${\delta}15N$ signatures in the dry season. Mature $S.$ $dehaani$ appeared to feed on fresh leaves and other carbon sources, such as immature individuals or fish, in addition to detrital sediment, which appeared to be the main carbon source for immature crabs. Principal component analysis of fatty acid biomarkers of $S.$ $dehaani$ showed a clear difference between immature individuals (10-30 mm) and mature ones (larger than 30 mm), indicating that the main food source for immature crabs was detrital sediment, whereas mature crabs foraged plants in addition to consuming detrital sediment. On the basis of our results from stable isotope and fatty acid analyses, mature $S.$ $dehaani$ appeared to feed on detrital sediment and fresh leaves of $S.$ $subfragilis$ in summer in addition to engaging in cannibalism of immature individuals.