Production of Healthy Shrimp(Penaeus chinensis, Penaeus japonicus) Seedling Free from White Spot Baculovirus(WSBV)

White Spot Baculovirus(WSBV) 미감염 새우(Penaeus chinensis, Penaeus japonicus) 종묘 생산

For the production of healthy shrimp(Penaeus chinensis, Penaeus japonicus) seedlings free from the white spot baculovirus(WSBV), we tried to disinfect shrimp eggs with iodine. A relative hatching rate of over 50% has resulted from the treatments of fertilized eggs of fresh shrimps(P. chinensis) with an effective povidon-iodine concentration of 20-200 ppm for 30 seconds and 60 seconds, respectively. The 60 sec treatment group with a 20 ppm effective povidon-iodine concentration showed nearly a 50% relative hatching rate. The over 50 ppm treatment group, however, did not result in hatching show a relative hatching rate. These hatching rates were similar to those in the fertilized eggs of kuruma prawns treated with povidon-iodine solutions. In case of washing fertilized eggs with UV-irradiated seawater 33.3% relative hatching rate in fresh shrimps was much lower than 55.2% in kuruma shrimps. Treatment fertilized eggs of 2 species of shrimp with 200 ppm iodine for 30 sec did not affect the survival rate of the larvae until the early stage of the post larvae even though the hatching rates were low. In order to produce healthy shrimp seedlings non-infected by WSBV, the shrimp eggs should be washed with UV-irradated seawater and sterilized with a low povidon-iodine concentration for a short time.

White Spot Baculovirus(WSBV) 미감염 새우 건강 종묘를 생산하기 위하여 난의 요오드 소독을 실시하였다. 유효 요오드농도 20,50,100,200 ppm에서 각 시험구의 대하(Penaeus chinensis) 수정난을 각각 30,60초 처리한 결과 각 농도의 30초 처리구에서는 50%이상의 상대부화율을 보였고, 60초 처리구에서는 20ppm 처리구에서만 약 50% 상대 부화율을 보인 반면 50 ppm이상에서는 부화율이 거의 없었다. 보리새우(Penaeus japoni cus)에서도 비슷한 결과를 보였다. 대하의 경우 세란만 실시한 대조구의 부화율은 33.3%이었지만, 보리새우는 55.2%로 상대적으로 높았다. 부화후 생존율을 보면 수정난의 소독으로 부화율은 낮아지지만 수정란 소독후 부화된 유생은 초기생육에는 문제점이 없었다. WSBV 미감염 건강종묘를 생산하기 위해서는 수정난을 세척하고 저농도의 요오드를 짧은 시간에 소독해야 한다.