• Journal of Nutrition and Health
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• 제12권1호
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• pp.31-41
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• 1979

Free amino acid in ethanol extracts and total amino acids in hydrolysates of eleven species of edible mushrooms were analyzed and determinated the contents five kind of new amino acid by means of amino acid autoanalyzer and gas liquid chromatography. The result obtained from this study are as follows. 1) Five kind of new amino acid turned out to be ${\alpha}$-aminobutyric acid, allo-isoleucine, ethanolamine, $\gamma$-aminobutyric acid and ornithine. 2) By means of amino acid autoanalyzer, the monoethanolamine was identified on the chromatogram ahead of alanine, ${\alpha}$-aminobutyric acid between peak of threonine and glycine, allo-isoeleucine between peak of valine and leucine, isoleucine, ${\gamma}$-aminobutyric acid followed by proline between peak of leucine, isoleucine and methionine and ornithine between peak of phenylalanine and tyrosine 3) By means of Gas liquid chromatography, the ${\alpha}$-aminobutyric acid was identified on the chromatogram between peaks of alanine and valine, allo-isoleucine between peaks of methionine and isoleucine, monoethanolamine followed by ${\gamma}$-aminobutyric acid between peaks of phenylalanine and ammonia, ornithine between the peaks of ammonia and lysine. 4) Of five amino acids which were identified, ornithine was the highest of its content in the mushroom extracts, and allo-isoleucine, ethanolamine, and ${\gamma}$-aminobutyric acid came next in decreasing order. 5) Also which were identified, ornithine was the highest of its content in the hydrolysates, and ${\alpha}$-aminobutyric acid, ${\gamma}$-aminobutyric acid, allo-isoleucine came next in decreasing order, ethanol extracts and hydrolysates of Auriculariaauricula-Judae(Fr.) $Qu\acute{e}l$ species didn't contain any of five kind of new amino acid. Ornithine also was the highest in the hydrolysates of ll mushrooms.

• Proceedings of the Korea Water Resources Association Conference
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• 한국수자원학회 2011년도 학술발표회
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• pp.147-147
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• 2011

이상기후 및 극한 홍수 발생빈도의 증가 등으로 인해 많은 수공 구조물이 붕괴 위험에 노출되어 있다. 사전 피해 예방 및 경감을 위해 다양한 수공구조물의 붕괴 현상에 대하여 수리실험적 접근방법을 통한 현상이해 및 예상결과 비교 검증이 필요하다. 그 중에서 제방붕괴에 대한 수리실험은 수치모의를 통한 분석의 어려움 때문에 대부분 모형실험을 통해서 이루어지고 있는 실정이다. 본 연구에서는 실제규모의 제방붕괴 선행실험의 측정결과를 활용하여 실험설계에 이용 하였다. 모형은 실험공간의 규모를 고려하여 축척을 1:10 으로하고, 하도내 흐름 안정을 위해 수로의 길이 는 16, 저폭은 $b\geq10h$를 만족하는 하천으로 설계하여 b를 3m로 설정 하였다. Fr수는 0.29로 원형과 동일하게 하고, 그에 따른 유량 ( )는 0.538m/s로 하였다. 실제 모형 제작에서는 현장 실험실의 펌프용량에 따른 가용유량 ($Q__{max}$)의 제약에 따라 수로가 직선이고 좌우가 대칭인 점을 감안하여 폭을 1/2로 절단 하고 유량은 $0.269m^3/s$(Q/2)를 공급하였다. 위와 같이 모형제작을 위한 실험 설계시 현장 여건을 고려하여 모형을 변형할 경우 EIM(Experimental Information Modeling)을 이용한 수리영향에 대한 분석을 통한 설계검증이 필요하다. FLOW-3D를 이용한 3차원 수치모의를 통하여 동일 지점에서의 유속과 수심을 분석하여 흐름양상을 비교 하였으며, 유속과 수심의 측정위치는 그림 1에 도시하였다. 수치모의 결과 측정지점에서의 수위가 하도 바닥을 기준으로 0.25m로 동일할 경우 수로 단면에 대한 유속 분포가 제방을 기준으로 2b/3까지는 유사한 경향을 보였다. 그 결과, $b\geq10h$인 수로에서 제방붕괴를 위한 모형 설계시 하도 폭을 1/2만 만들 경우에도 실험의 신뢰성이 확보된다는 것을 확인하였다.

• Korean Journal of Medicinal Crop Science
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• 제14권4호
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• pp.212-216
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• 2006

Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of $0.1\;{\mu}g$. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by $^1H-NMR$, $^{13}C-NMR$ and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration $100\;{\mu}g/3\;ml$. That was identified to be 3,5,7-trihydroxy-2'-methoxyflavanone by spectrophotometric analysis using $^1H-NMR,\;^{13}C-NMR$ and Mass spectrophotometry.

• Proceedings of the Korean Vacuum Society Conference
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• 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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• pp.465-465
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• 2014

증식블랑켓모듈(TBM, Test Blanket Module)을 개발하여 왔다. 이 두 증식블랑켓모듈은 모두 헬륨냉각을 기반으로 개발 되어왔으며 이에 따라, 헬륨순환기, 헬륨히터 및 헬륨열교환기 등에 대한 기본적인 연구가 수행되었다. 이후 2012년 고체형 증식블랑켓모듈을 ITER TBM 개념으로 주도하기로 결정함에 따라, HCCR (Helium Cooled Ceramic Reflector) TBM의 보조계통인 하나인 헬륨냉각계통(HCS, Helium Cooling System)에 대한 개발이 본격적으로 이루어졌다. 한국원자력연구원에서는 HCCR TBM의 냉각성능을 만족하기 위하여 8 MPa, 1.5 kg/s 및 $300/500^{\circ}C$ (입구/출구 온도)의 운전조건을 갖는 헬륨냉각계통의 설계를 완료하였다. 설계된 헬륨냉각계통은 HCCR TBM에서 회수된 약 $450^{\circ}C$의 헬륨을 열회수기(recuperator)기와 냉각기를 통해 상온으로 냉각시킨 후, 필터를 통해 헬륨을 여과시킨다. 여과된 헬륨은 헬륨순환기에 의해 가압되어 열회수기를 다시 지나 $300^{\circ}C$ 이상으로 가열된다. 가열된 헬륨은 열회수기를 지나지 않는 상온의 헬륨과 혼합되어 최종적으로 HCCR TBM의 입구온도 조건인 $300^{\circ}C$로 맞추어 HCCR TBM에 공급된다. 이러한 열회수기 중심으로 '${\infty}$' 모양의 자가 교차로 설계된 헬륨냉각계통은 고온영역과 저온영역으로 냉각회로를 구분하여 순환기, 필터 및 각종 계측기의 운전온도 환경을 상온으로 유지시킬 수 있어 운전 및 유지보수 관점에서 이점이 있다. HCCR TBM의 헬륨냉각계통 설계 및 핵심 기기를 실증하고, 운전 경험을 쌓기 위하여 헬륨공급장치(HeSS, Helium Supply System)를 헬륨유량기준 1/3 규모(0.5 kg/s)로 구축하였으며, '14년까지 HeSS를 실증규모로 업그레이드 하기 위하여 80기압 환경에서 압축비 1.1, 유량 1.5 kg/s의 성능을 내는 헬륨순환기를 설치할 예정이다. 현재 구축된 1/3 규모 HeSS는 국내 구축된 전자빔 고열부하 시험 장비인 KoHLT-EB (Electron Beam)와 연계되어 HCCR TBM의 일차벽(플라즈마 대향부품)을 검증할 예정이며, 이를 통해 얻어진 열수력 DB는 현재 개발중인 핵융합로 안전해석코드인 GAMMA-FR 검증에 활용될 계획이다.

• The Korean Journal of Mycology
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• 제28권1호
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• pp.26-31
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• 2000

We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Flammulina velutipes [(Curt: Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.

• The Korean Journal of Mycology
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• 제28권1호
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• pp.11-15
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• 2000

For artificial cultivation of Phellinus pini (Thore. Fr.) Ames, we conducted some study on mycelium growth and optimum condition for fruitbody formation. The optimum condition for mycelial growth was $25{\sim}30^{\circ}C$ at pH $5.0{\sim}6.0$. Optimum sawdust media were oak sawdust+willow sawdust+rice bran (4.5:4.5:1, V/V) and oak sawdust+pine sawdust+rice bran (4.5:4.5:1, V/V) and the optimum spawn incubation period was about $33{\sim}34$ days. Mycelial growth in the inner portion of oak log was 40 mm after 60 days and duration for first fruitbody primordia formation was about 110 days after inoculation.

• Korean Journal of Materials Research
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• 제17권3호
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• pp.152-159
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• 2007

When the electronics are tested with thermal shock for Pb-free solder joint reliability, there are temperature conditions with use environment but number of cycles for test don't clearly exist. To obtain the long term reliability data, electronic companies have spent the cost and times. Therefore this studies show the test method and number of thermal shock cycles for evaluating the solder joint reliability of electronic components and also research bonding strength variation with formation and growth of intermetallic compounds (IMC). SMD (surface mount device) 3216 chip resistor and 44 pin QFP (quad flat package) was utilized for experiments and each components were soldered with Sn-40Pb and Sn-3.0 Ag-0.5 Cu solder on the FR-4 PCB(printed circuit board) using by reflow soldering process. To reliability evaluation, thermal shock test was conducted between $-40^{\circ}C\;and\;+125^{\circ}C$ for 2,000 cycles, 10 minute dwell time, respectively. Also we analyzed the IMCs of solder joint using by SEM and EDX. To compare with bonding strength, resistor and QFP were tested shear strength and $45^{\circ}$ lead pull strength, respectively. From these results, optimized number of cycles was proposed with variation of bonding strength under thermal shock.

• The Korean Journal of Physiology and Pharmacology
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• 제1권4호
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• pp.377-384
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• 1997

To investigate the properties of ryanodine binding sites of the bird skeletal SR vesicles, SDS PAGE, purification of RyR, and $[^3H]ryanodine$ binding study were carried out in the SR vesicles prepared from the chicken pectoral muscle. The chicken SR vesicles have two high molecular weight (HMW) protein bands as in eel SR vesicles on SDS PAGE. The HMW bands on SDS PAGE were found in the $[^3H]ryanodine$ peak fraction $(Fr_{3-5})$ obtained from the purification step of the ryanodine receptor protein. Bmax and KD of the chicken $[^3H]ryanodine$ binding sites were 12.52 pmol/mg protein and 14.53 nM, respectively. Specific $[^3H]ryanodine$ binding was almost maximal at $50{\sim}100$ ${\mu}M$ $Ca^{2+}$, but was not increased by 5 mM AMP and not inhibited by high $Ca^{2+}$. Binding was significantly inhibited by $20{\sim}100$ ${\mu}M$ ruthenium red and 1 mM tetracaine, but slightly inhibited by $Mg^{2+}$. From the above results, it is suggested that chicken SR vesicles have the ryanodine binding sites to which the binding of ryanodine is almost maximal at $50{\sim}10$ ${\mu}M$ $Ca^{2+}$, is significantly inhibited by ruthenium red and tetracaine, slightly inhibited by $Mg^{2+}$, but not affected by AMP and not inhibited by high $Ca^{2+}$.

• The Plant Pathology Journal
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• 제29권2호
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• pp.168-173
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• 2013

Apple ring rot disease, caused by Botryosphaeria dothidea (Moug. ex. Fr) Ces. et de Not., is one of the most important diseases on apple fruits. In this study, strain 9001 isolated from healthy apple fruits from an infested orchard was evaluated for its biocontrol activity against apple ring rot in vitro and in vivo. Strain 9001 showed obvious antagonistic activity to B. dothidea YL-1 when plated on potato dextrose agar. Soaking healthy apples in the bacterial suspensions of strain 9001 prior to artificial inoculation of fungal pathogen resulted in a dramatic decrease in disease incidence when compared to the control. Moreover, either field application in the growth season or postharvest treatment of apples from infected orchards with bacterial suspensions of strain 9001 resulted in significantly reduced disease incidence within the storage period for 4 months at room temperature. Based on the phylogenetic analysis of 16S rRNA and the gyrA gene, strain 9001 was identified as Bacillus amyloliquefaciens. These results indicated that B. amyloliquefaciens 9001 could be a promising agent in biocontrol of apple ring rot on fruit, which might help to minimize the yield loss of apple fruit during the long postharvest period.

• Biomolecules & Therapeutics
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• 제20권2호
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• pp.234-238
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• 2012

Propofol is an anesthetic commonly used to provide sedation or to induce and maintain an anesthetic stated. However, there are reports which indicate propofol may cause psychological dependence or be abused. In the present study, we used various behavioral tests including climbing test, jumping test, conditioned place preference, and self-administration test to assess the dependence potential and abuse liability of propofol compared to a positive control (methamphetamine) or a negative control (saline or intralipid). Among the tests, the conditioned place preference test was conducted with a biased method, and the selfadministration test was performed under a fixed ratio (FR) 1 schedule, 1 h per session. No difference was found in the climbing test and jumping test, but propofol (30 mg/kg, i.p.) increased the rewarding effect in the conditioned place preference test, and it showed a positive reinforcing effect compared to the vehicle. These results indicate that propofol tends to show psychological dependence rather than physical dependence, and it seems not to be related with dopaminergic system.