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Isolation of Antioxidative Compound from Scutellaria baicalensis G.  

Kim, Seok-Chang (KT&G Central Research Institute)
Ahn, Kun-Seok (Korea United Pharm Inc.)
Park, Chae-Kyu (KT&G Central Research Institute)
Jeon, Byeong-Seon (KT&G Central Research Institute)
Lee, Jong-Tae (KT&G Central Research Institute)
Park, Won-Jong (Dept. of Food Engineering, Kongju Natl. Univ.)
Publication Information
Korean Journal of Medicinal Crop Science / v.14, no.4, 2006 , pp. 212-216 More about this Journal
Abstract
Root of Scutellaria baicalensis G. was extracted with methanol and water to give the yield of 30.0% in order to find the antioxidant substance. The extract was fractionated with diethyl ether, n-butanol and water to test the inhibitive activity against xanthine oxidase. Three fractions inhibited the activities of xanthine oxidase by 48.2%, 10.2% and 2.8%, respectively, at the amount of $0.1\;{\mu}g$. A component that exhibited strong inhibition of xanthine oxidase was isolated from diethyl ether fraction (SE Fr.) by silica gel column chromatography and HPLC, and then identified by $^1H-NMR$, $^{13}C-NMR$ and MS spectrophotometry. EDA (Electron Donating ability) of the compound was 28.5% at the concentration $100\;{\mu}g/3\;ml$. That was identified to be 3,5,7-trihydroxy-2'-methoxyflavanone by spectrophotometric analysis using $^1H-NMR,\;^{13}C-NMR$ and Mass spectrophotometry.
Keywords
Scutellaria baicalensis G; xanthine oxidase; 3,5,7-trihydroxy-2'-methoxyflavanone;
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