• BMB Reports
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• v.57 no.1
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• pp.30-39
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• 2024

Directed evolution (DE) of desired locus by targeted random mutagenesis (TRM) tools is a powerful approach for generating genetic variations with novel or improved functions, particularly in complex genomes. TRM-based DE involves developing a mutant library of targeted DNA sequences and screening the variants for the desired properties. However, DE methods have for a long time been confined to bacteria and yeasts. Lately, CRISPR/Cas and DNA deaminase-based tools that circumvent enduring barriers such as longer life cycle, small library sizes, and low mutation rates have been developed to facilitate DE in native genetic environments of multicellular organisms. Notably, deaminase-based base editing-TRM (BE-TRM) tools have greatly expanded the scope and efficiency of DE schemes by enabling base substitutions and randomization of targeted DNA sequences. BE-TRM tools provide a robust platform for the continuous molecular evolution of desired proteins, metabolic pathway engineering, creation of a mutant library of desired locus to evolve novel functions, and other applications, such as predicting mutants conferring antibiotic resistance. This review provides timely updates on the recent advances in BE-TRM tools for DE, their applications in biology, and future directions for further improvements.

• Journal of Korean Library and Information Science Society
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• v.42 no.4
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• pp.181-201
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• 2011

The recent diversification of society surrounding libraries is influencing the services of libraries. This study suggested the directions of public library services from the aspect of the universal design based on the analysis of related systems and cases to provide all users with universal services in accord with the nature of public libraries amid rapidly changing social environment. This study suggests four directions: the "universal design" which is the keyword for pursuing universality of public library services; the pursuit of economic value of public library services based on the universal design; the universal design for the improvement of perception of public library services; change in policies and systems related to public library services through the application of the universal design.

• Archives of Plastic Surgery
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• v.38 no.6
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• pp.879-882
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• 2011

Purpose: Superior orbital fissure syndrome is a rare neurological complex. Superior orbital fissure syndrome may result from a variety of inflammatory, infectious, neoplastic, iatrogenic, traumatic, vascular cause. The author report a patient who suffered from superior orbital fissure syndrome after inferior orbital wall reduction. Methods: A 26-year-old female suffered from inferior orbital wall fracture with inferior gaze limitation and orbital soft tissue herniation. On posttrauma 10 day, inferior orbital wall was reduced using endoscope and porous polyethylene ($Medpor^{(R)}$) was inserted. On immediate postoperation, she reported that extraocular movement was limited in almost any directions. She underwent exploration surgery to release the presence of extraocular muscle impingement. But, there was no observation of extraocular muscle impingement. On postoperative one day, high-dose steroid therapy was started to release superior orbital fissure syndrome which was defined in postoperative computed tomography. Results: After one month of high-dose steroid therapy, extraocular movement limitations improved progressively in all directions. In four months, extraocular movement recovered completely. Conclusion: Superior orbital fissure syndrome may occur after surgical procedure of orbital wall reduction. Prompt diagnosis and treatment with mega-dose corticosteroid is an effective option for avoiding disaster from compressive syndrome.

• Journal of Environmental Impact Assessment
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• v.16 no.2
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• pp.157-168
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• 2007

In case there is a need to run the multi-year urban scale air qulaity model, it is a difficult task due to the computational demand, requiring the statistical approach for the long time atmospheric environmental assessment. In an effort to approach toward long term urban assessment, the sixteen synoptic meteorological conditions are statistically classified from the estimated geostrophic wind speeds and directions of 850 hPa geopotential height field during 2000 ~ 2005. The geostrophic wind directions are subdivided into four even intervals (north, east, south, and west), geostrophic wind speeds into two classes(${\leq}5m/s$ and >5m/s), and daily mean cloud amount into 2 classes(${\leq}5/10$ and >5/10), which result into sixteen classes of the synoptic meteorological cases for each season. The frequency distributions for each 16 synoptic meteorological case are examined and some discussions on how these synoptic classifications can be used in the environmental assessment are presented.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.3
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• pp.253-263
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• 2020

This study aims to analyze of existing concepts of female entrepreneurship, evaluation of women's entrepreneurship in Kazakhstan and development of recommendations for its development in the country. This paper begins by reviewing research on female entrepreneurship. In this research, authors proposed the methodological tools based on systematic approach using economic and statistic methods and 5Ms concept. Analysis algorithm consists of four stages: evaluation of women entrepreneurship scale and business directions, analysis of employment in women's enterprises and business environment in Kazakhstan. The main limitation of this study was the lack of some gender-related indicators on the development of entrepreneurship. Further, the obtained results showed strong and weak points of female entrepreneurship in Kazakhstan. According to the results, there has observed increase in contribution of women in social-economic development of the republic, women's entrepreneurial activity is increasing, and the number of enterprises headed by them is growing. However, the growth of female entrepreneurship occurs while maintaining their insignificance in terms of turnover and number of employees. There is a pronounced industry specificity of female entrepreneurship: the service sector. In accordance with this, there were identified priority areas and there were developed recommendations for the development of female entrepreneurship in Kazakhstan.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.4 s.97
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• pp.457-464
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• 2005

A three-dimensional (3-D) method of analysis is presented for determining the free vibration frequencies and mode shapes of thick, complete (not truncated) conical shells of revolution, Unlike conventional shell theories, which are mathematically two-dimensional (2-D). the present method is based upon the 3-D dynamic equations of elasticity. Displacement components $u_{r},\;u_{z},\;and\;u_{\theta}$ in the radial, axial, and circumferential directions, respectively, are taken to be sinusoidal in time, periodic in , and algebraic polynomials in the r and z directions. Potential (strain) and kinetic energies of the conical shells are formulated, the Ritz method is used to solve the eigenvalue problem, thus yielding upper bound values of the frequencies by minimizing the frequencies. As the degree of the polynomials is increased, frequencies converge to the exact values. Convergence to four-digit exactitude is demonstrated for the first five frequencies of theconical shells. Novel numerical results are presented for thick, complete conical shells of revolution based upon the 3-D theory. Comparisons are also made between the frequencies from the present 3-D Ritz method and a 2-D thin shell theory.

• Journal of Korean Society of Rural Planning
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• v.9 no.1 s.18
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• pp.55-64
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• 2003

The objective of the study is to suggest planning directions for practical use and to find the conditions of tourist attractions in rural villages founded on city-dweller's preference. Both a questionnaire and a field survey were used for the study. Selected were four villages such as Oryan-ri, gachun-ri, Junggi-ri, and Gyochon-ri. The results of the study are as follows: City-dweller's valued on natural environment resources in rural tourism and felt uncomfortable to accommodations. The scoring system reflecting functions of six resources showed that common problems encountered in rural tourism were lacks of people's organization, and human exchange mind, and tourism-related leasure places and facilities. Based upon the results, some measures to develop villages were suggested.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.16 no.10 s.115
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• pp.1024-1035
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• 2006

A three-dimensional (3-D) method of analysis is presented for determining the free vibration frequencies and mode shapes of thick, complete (circumferentially closed), circular rings with an elliptical or circular cross-section. Displacement components $u_r,\;u_\theta\;and\;u_z$ in the radial, circumferential, and axial directions, respectively, are taken to be periodic in ${\theta}$ and in time, and algebraic polynomials in the r and z directions. Potential (strain) and kinetic energies of the circular rings are formulated, and upper bound values of the frequencies are obtained by minimizing the frequencies. As the degree of the polynomials is increased, frequencies converge to the exact values. Convergence to four-digit exactitude is demonstrated for the first five frequencies of the rings. Novel numerical results are presented for the circular rings having an elliptical cross-section based upon 3-D theory. Comparisons are also made between the frequencies from the present 3-D Ritz method and ones obtained from thin and thick ring theories, experiments, and another 3-D method.

• Korean Journal of Applied Biomechanics
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• v.16 no.2
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• pp.175-192
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• 2006

The purpose of this study was to analyze the current status and suggest tasks of future directions for development of the Korean Society of Sport Biomechanics(KSSB). KSSB was established in the 22th of Oct.,1988. It is one of the great National Society recognized by the Korean Research Foundation, and consists of almost 350 members over national Sport Biomechanists. Two roles-functions of KSSB are as follows; one is an Independent Society by KSSB, the other is a sub-Society under Korean Alliance for Health, Physical Education, Recreation and Dance(KAHPERD) run by 15 sub-Societies. The 2 great strategies toward all over the world of KSSB were Localization and Globalization. The grand four projects of KSSB in $2005{\sim}2006yrs$ are as followers: 1) The rationalization of organization-operating and decentralization, 2) The international levels of society, 3) The internationalizaton of quality of Korean Journal of Sport Biomechanics, and 4) The presentation award by quarterly and double-movements of members of KSSB. The tasks of future direction for development of KSSB are as followers : The localization and globalization of KSSB( see above as figure 4, table 12, 13, 14).

• Journal of Technologic Dentistry
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• v.7 no.1
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• pp.5-12
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• 1985

This experimental study is perform to study the effect of the direction for the centrifugal force in operating centrifugal casting machine. In order to investigate the effect of direction for centrifugal force action on the casting ability 20 pieces of specimen are produced including one vertical direction and four horizontal directions. Casting shows us that the 15 pieces of specimen among them are included within $\pm$16. The results obtained through the comparison with 5 groups of the casting ability are as follows: 1. In the experiment of the casting, statistically significant difference are found between the vertical direction and the group of horizontal direction, namely, the direction of 3, 6, 9 and 12 o'clock, and also statistically there are significant differences between the direction of 9 o'clock and of 6 o'clock, and between the direction of 9 o'clock and of 12 o'clock which lie between the groups of horizontal directions. 2. The degree of casting ability is showing us on an average, 23.80 in the vertical direction. And also in the group of horizontal direction it prove in turn 15.60 in the direction of 9 o'clock, 14.47 of 3 o'clock, 13.30 of 12 o'clock, and 12.80 of 6 o'clock.