• Title/Summary/Keyword: Forest Activity

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Antifungal Activity of Extracts from Black Heartwood of Cryptomeria japonica against Dermatophytes (삼나무 흑심재 추출물의 피부사상균에 대한 항진균 활성)

  • Lee, Su-Yeon;Gwak, Ki-Seob;Lee, Jun-Jae;Yeo, Hwanmyeong;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.3
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    • pp.265-273
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    • 2009
  • The aim of this study was to investigate the antifungal activitiy of extracts from different parts of Cryptomeria japonica against Trichophyton mentagrophytes, Trichophyton rubrum and Microsporum gypseum. Disc diffusion and agar dilution methods were used for determining the antifungal activity of extracts, and their major constituents were analyzed by GC/MS. Black heartwood extract among the extracts showed the highest antifungal activity against dermatophytes. TLC was used for fractionating the effective fraction from the black heartwood extract with n-hexane and chloroform as developing solvents, and then antifungal activity of each fraction was examined against dermatophytes. As a result, seventh fraction showed the highest antifungal activity among nine fractions. The major constituent of the seventh fraction determined by GC/MS was expected to be sesquiterpene, damascone with 2 more carbon atoms. The other constituents were also identified as elemol, eudesmol and hinokione.

Investigation on Forest Soil Dynamics at Onsan Industrial Estate and Mt. Mani by the Assay of Dehydrogenase Activity, Denitrifying and Sulfur-Reducing Bacteria (탈수소효소(脫水素酵素), 탈질균(脫窒菌) 및 황산환원균(黃酸還元菌)의 정량(定量)을 통(通)한 온산공단(溫山工團)과 마니산(摩尼山) 산림토양(山林土壤)의 동태(動態) 조사(調査))

  • Park, Hyun
    • Journal of Korean Society of Forest Science
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    • v.87 no.1
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    • pp.106-112
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    • 1998
  • This study was conducted to figure out the relationships among soil chemical properties and bacterial biomass related to denitrification and sulfur-reducing and the activity of dehydrogenase, and ultimately to consider the usefulness of dehydrogenase activity as a tool for evaluating the dynamics of forest soil ecosystem. Four sites were selected for the collection of soil samples within two regions(Onsan industrial estate as a polluted region and Mt. Mani at Kanghwa island as a clean area) with two forest types (coniferous and deciduous stands). The soils of Mt. Mani showed higher amount of organic matter, total nitrogen and available phosphorus than those collected from Onsan industrial estate, which indicated that the soils were more beneficial for microbial growth than those of Onsan. The dehydrogenase activity was more sensitive than the denitrifying bacteria or sulfur-reducing bacteria since the activity was significantly different between the regions and season while the two bacterial biomass were not significantly different between the two regions. In addition, the dehydrogenase activity showed relatively high correlation coefficients with organic matter(r=0.53, p=0.004), total nitrogen(r=0.41, p=0.008) and C/Ava. P-ratio(r=-0.52, p=0.001), which was thought to be closely related with microbial activity. Thus, the dehydrogenase activity was thought to be a useful index of soil ecosystem dynamics with considering that the technique need to be applied with the same soil texture for the comparison of the activity as other researchers indicated.

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Difference in Growth, SOD Activity and MDA Content Between Ozone Tolerant and Sensitive Families of Open-Pollinated Pinus densiflora (소나무 풍매차대묘의 오존 내성 및 민감성 가계간 생장, SOD 활성 및 MDA 함량 차이)

  • Lee, Jae-Cheon;Oh, Chang-Young;Han, Sim-Hee;Kim, Jang-Su
    • Journal of Korean Society of Forest Science
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    • v.95 no.3
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    • pp.323-327
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    • 2006
  • This study was conducted to understand response of damage and tolerance to ozone between ozone tolerant and sensitive families of P. densiflora which had been selected by the based on visual injury and growth. Five individuals were selected in each group, and were exposed to 100ppb ozone for 90 days. Every 30 days after ozone fumigation, diamter at root collar (ORC), superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were measured. In early state of ozone fumigation tolerant families showed three times higher relative DRC growth rate than sensitive families. And during whole fumigation period growth rate of tolerant families was superior to growth rate of sensitive families. SOD activity in sensitive families was increased 30 days after fumigation, and in accordance with fumigation period extended it was decreased continuously. MDA content in ozone treatment showed higher than in control, and MDA content of tolerant families was higher than that sensitive families. In our results tolerant families has higher antioxidative activity than sensitive families. Therefore tolerant families can restrain lipid peroxidation and damage on physiological activity.

Study on the Relationship between the Structure and Antioxidant Activities of Chalcones

  • Park, Youngki;Lee, Hak-Ju;Lee, Wi Young;Ahn, Jin-Kwon;Hwang, Byung-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.2
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    • pp.88-94
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    • 2006
  • The purpose of this study is to examine the relationship between antioxidant activities and chemical structures of various chalcones. Twenty-two chalcones were assessed for their radical scavenging activity using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Of 22 compounds tested, the most active on DPPH radical was 2',4-dihydroxy-3,3'5-trimethoxy-5'-propylchacone (4) (72.6% at 100 ppm). It was followed by 3',4'-dihydroxy-3,4,5-trimethoxy-6'-methylchalcone (6), 2',4,4'-trihydroxy-3-methoxy-5-propenylchalcone (7) and 2',4,4'-trihydroxy-3,5-dimethoxychalcone (13). Based on the results, we concluded that the scavenging activity is controlled by the number and the position of the substitution in the compound.

Biological Characteristics of Lycorma delicatula and the Control Effects of Some Insecticides (꽃매미(Lycorma delicatula)의 생태 특성 및 약제 살충 효과)

  • Park, JI-Doo;Kim, Min-Young;Lee, Sang-Gil;Shin, Sang-Chul;Kim, Jun-Heon;Park, Il-Kwon
    • Korean journal of applied entomology
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    • v.48 no.1
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    • pp.53-57
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    • 2009
  • Biological Characteristics of Lycorma delicatula and insecticidal activity of some insecticides against nymphs of L. delicatula was investigated. Nymph of L. delicatula had 4 instars, and color of body was black. There were white spots on the body of 1st-3rd nymph. Upper body became red at 4th nymph. Adult forewings were brownish, and had black spots. Color of hind wing were red. The egg mass was covered with a yellowish brown secretion. The adult of L. delicatula emerged once a year. Among test insecticides, deltamethrin 1% EC and fenitrothion 50% EC showed very quick and strong insecticidal activity against the 2nd-3rd nymphs of L. delicatula. Imidacloprid 4% SL and clothianidin 8% SC showed 100% insecticidal activity at 24h after treatment. Thiacoprid 10% SC revealed the weakest insecticidal activity among the insecticides tested.

Anti-inflammatory Effect of Branches Extracts from Quercus mongolica in LPS-induced RAW264.7 Cells

  • Eo, Hyun Ji;Park, Youngki;Kang, Jin Taek;Park, Gwang Hun
    • Korean Journal of Plant Resources
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    • v.32 no.6
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    • pp.698-704
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    • 2019
  • Quercus mongolica (QM), which belongs to fagaceae, is one of the oak native to Korea. We evaluated the anti-inflammatory effect of branches extracted with 70% ethanol of QM (QM-B) and elucidated the potential signaling pathway in LPS-induced RAW264.7 cells. The QM-B showed anti-inflammatory activity through inhibition of NO production. The QM-B dose-dependently suppressed NO production by inhibiting iNOS, COX-2 and IL-6 expression in LPS-induced RAW264.7 cells. The QM-B inhibited the degradation and phosphorylation of IκB-α and NF-κB activation. The QM-B suppressed the phosphorylation of p38 and ERK1/2. Also, the QM-B increased HO-1 expression. These results suggested that QM-B may utilize anti-inflammatory activity by suppressing NF-κB and MAPK signaling pathway and inducing HO-1 expression indicated that the QM-B can be used as a natural anti-inflammatory drugs.

Extracellular Enzyme Activities of the Monokaryotic Strains Generated from Basidiospores of Shiitake Mushroom

  • Kwon, Hyuk-Woo;Back, In-Joung;Ko, Han-Gyu;You, Chang-Hyun;Kim, Seong-Hwan
    • Mycobiology
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    • v.36 no.1
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    • pp.74-76
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    • 2008
  • To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, $\beta$-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.

Antidiabetic Activities of Korean Red Pine (Pinus densiflora) Inner Bark Extracts

  • Min, Hee-Jeong;Kim, Eun-Ji;Shinn, Seong-whan;Bae, Young-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.47 no.4
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    • pp.498-508
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    • 2019
  • This study was carried out to investigate the potential of Korean red pine (Pinus densiflora) inner bark extracts as an antidiabetic agent. The ethyl acetate soluble fraction of the bark extracts was chromatographed on a Sephadex LH-20 column to yield five compounds, which structures were elucidated by NMR spectroscopy. The isolated compounds were (+)-catehin, (-)-epicatechin, taxifolin, taxifolin-3'-O-${\beta}$-D-(+)-glucose and $\tilde{n}$-courmaric acid. The antidiabetic activity of the different fractions, including the crude extracts and isolated compounds, was evaluated by ${\beta}$-cells insulin secretion and glucose uptake in skeletal muscle cells. The insulin secretion was 128% for taxifolin at $25{\mu}g/mL$. However, the other samples had no effect on this test. For the glucose uptake activity assay, $1{\mu}M$ insulin and 2 mM metformin were used as controls. Both the crude extract and taxifolin showed relatively low activity values, but the other samples yielded glucose uptake values over 260%. ${\rho}$-courmaric acid showed the highest uptake (270%). The results confirmed that Korean red pine extracts may be used as a hypoglycemic agent.

Effects of Forest Bathing at Samdasu Forest in Jeju on Pulse and Stress (제주 삼다수 숲 산림욕이 맥박과 스트레스에 미친 효과)

  • Sin, Bang Sik;Lee, Jae H.;Song, Kyu J.;Shin, Aran;Lee, Hyung H.
    • Journal of Naturopathy
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    • v.10 no.1
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    • pp.33-41
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    • 2021
  • Purposes: We studied whether forest bathing is effective in improving the stress of the human body. Therefore, we investigated changes in pulse rate, stress index, and autonomic nervous system after 2 hours of forest bathing on Jeju Samdasu Forest Methods : The study was conducted before and after forest bathing through heart rate variability and questionnaire. Results: After forest bathing, the average pulse rate of the experimental group was significantly increased. The stress index significantly decreased in the post-test in the experimental group. The cumulative stress, sympathetic activity (LF), parasympathetic activity (HF) change, and LF/HF ratio were slightly different from those of post-test in the experimental group, but were not statistically significant. In the survey of job stress average index and state characteristic anxiety index through questionnaire, it decreased significantly after forest bathing (p<.001). Conclusions: The Samdasu forest bathing has been proven effective in enhancing pulse and reducing stress, so it is evaluated that forest bathing is effective in promoting health.