Mycobiology

The Korean Society of Mycology (한국균학회)
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Extracellular Enzyme Activities of the Monokaryotic Strains Generated from Basidiospores of Shiitake Mushroom

  • Kwon, Hyuk-Woo (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Back, In-Joung (Department of Microbiology and Institute of Basic Sciences, Dankook University) ;
  • Ko, Han-Gyu (Forest Mushroom Research Institute, National Forest Cooperatives Federation) ;
  • You, Chang-Hyun (Forest Mushroom Research Institute, National Forest Cooperatives Federation) ;
  • Kim, Seong-Hwan (Department of Microbiology and Institute of Basic Sciences, Dankook University)
  • Published : 2008.03.31
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Abstract

To obtain basic information on the biochemical property of basidiospores of shiitake mushroom (Lentinula edodes), the ability of producing extracellular enzyme was assessed using a chromogenic plate-based assay. For the aim, amylase, avicelase, $\beta$-glucosidase, CM-cellulase, pectinase, proteinase, and xylanase were tested against monokaryotic strains generated from forty basidiospores of two different parental dikaryotic strains of shiitake mushroom, Sanjo-101Ho and Sanjo-108Ho. These two parental strains showed different degree of extracellular enzyme activity. No identical patterns of the degree of enzyme activity were observed between monokaryotic strains and parental strains of the two shiitake cultivars. The degree of extracellular enzyme activity also varied among monokaryotic strains of the two shiitake cultivars. Our results showed that dikaryotic parental strains of shiitake mushroom produce monokaryotic basidiospores having very diverse biochemical properties.

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References

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  1. Assessment of the Ability of Extracellular Enzyme Production in Hybrid Strains of Lentinula edodes by Chromogenic Reaction-based Plate Assay vol.39, pp.2, 2011, https://doi.org/10.4489/KJM.2010.39.2.099
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