• Molecules and Cells
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• 제45권1호
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• pp.26-32
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• 2022

Living cells generate, sense, and respond to mechanical forces through their interaction with neighboring cells or extracellular matrix, thereby regulating diverse cellular processes such as growth, motility, differentiation, and immune responses. Dysregulation of mechanosensitive signaling pathways is found associated with the development and progression of various diseases such as cancer. Yet, little is known about the mechanisms behind mechano-regulation, largely due to the limited availability of tools to study it at the molecular level. The recent development of molecular tension probes allows measurement of cellular forces exerted by single ligand-receptor interaction, which has helped in revealing the hitherto unknown mechanistic details of various mechanosensitive processes in living cells. Here, we provide an introductory overview of two methods based on molecular tension probes, tension gauge tether (TGT), and molecular tension fluorescence microscopy (MTFM). TGT utilizes the irreversible rupture of double-stranded DNA tether upon application of force in the piconewton (pN) range, whereas MTFM utilizes the reversible extension of molecular springs such as polymer or single-stranded DNA hairpin under applied pN forces. Specifically, the underlying principle of how molecular tension probes measure cell-generated mechanical forces and their applications to mechanosensitive biological processes are described.

• Smart Structures and Systems
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• 제30권4호
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• pp.397-410
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• 2022

It is essential to monitor the working conditions of bolt-connected joints, which are widely used in various kinds of steel structures. The looseness of bolts may directly affect the stability and safety of the entire structure. In this study, a guided wave-based method for bolt looseness detection and localization is presented for a joint structure with multiple bolts. SH waves generated and received by a small number (two pairs) of magnetostrictive transducers were used. The bolt looseness index was proposed based on the changes in the reconstructed responses excited by the time reversal signals of the measured unit impulse responses. The damage locations and local damage severities were estimated using the damage indices from several wave propagation paths. The back propagation neural network (BPNN) technique was employed to identify the local damages. Numerical and experimental studies were conducted on a lap joint with eight bolts. The results show that the total damage severity can be successfully detected under the effect of external force and measurement noise. The local damage severity can be estimated reasonably for the experimental data using the BPNN constructed by the training patterns generated from the finite element simulations.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2003년도 추계학술발표회초록집
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• pp.54-54
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• 2003

DLC films were deposited on Si(100) substrates by inductively coupled plasma (ICP) assisted chemical vapor deposition (CVD). A mixture of acetylene (C$_2$H$_2$) and argon (Ar) gases was used as the precursor and plasma source, respectively. The structure of the films was characterized by the Raman spectroscopy. Results from the Raman spectroscopy analysis indicated that the property change of the DLC films is due to the sp$^3$ and sp$^2$ ratio in the films under various conditions such as ICP power, working pressure and RF substrate bias. The hydrogen content in the DLC films was determined by an electron recoil detector (ERB). The roughness of the films was measured by atomic force microscope (Am). A microhardness tester was used for the hardness and elastic modulus measurement. The DLC film showed a maximum hardness of 37㎬. In this work, the relationship between deposition parameters and mechanical properties were discussed.

• PNF and Movement
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• 제20권1호
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• pp.125-134
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• 2022

Purpose: The aim of this study was to establish an association between grip strength and gait variability in the elderly. Methods: The participants in this experiment (n = 20) were aged 65 or older. Power grip and lateral pinch forces were obtained in grip strength tests, and spatiotemporal gait parameters were collected from IMU sensors during 6 min actual walking to test the gait of participants. The collected gait parameters were converted to coefficient of variation (CV) values. To confirm the association between grip strength and gait variability, a partial correlation analysis was conducted in which height, weight, and gait speed were input as controlling variables. Results: Grip power showed a significant negative correlation with the stride length CV (r = -0.52), and the lateral pinch force showed a significant negative correlation with the stance CV (r = -0.65) and swing CV (r = -0.63). Conclusion: This study reveals that gait variability decreases as grip strength increases, although height, weight, and gait speed were controlled. Thus, grip strength testing, a simple aging evaluation method, can help identify unstable gait in older adults at risk of falling, and grip strength can be utilized as a non-invasive measurement method for frailty management and prevention.

• 소성∙가공
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• 제32권6호
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• pp.360-366
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• 2023

This study is concerned with the surface quality of products according to the material and coating condition of the forming tool in incremental sheet forming. Three forming tools, SKD11 with and without diamond-like-coating (DLC) and polymer tool tip, were used to form conical and pyramidal geometries to take into account the influence of friction between the forming tool and the sheet on the surface quality including geometric accuracy of deformed samples. Each test was performed using SUS304 with a thickness of 0.4 mm according to different incremental depths per lap of 0.5 mm, 1.0 mm, and 1.5 mm for the contour tool path, considering the increase in normal force which is associated with the frictional behavior during local deformation. The surface quality was then investigated through surface roughness measured with KEYENCE VR-6000 and relative strain distribution including deformed shape analyzed with ARGUS which is a non-contact optical strain measurement system. Differences between 3D CAD surfaces and captured geometry from experiments were evaluated to compare the effect of friction on geometric accuracy. From comparisons of experimental results, it was revealed that the polymer-based tool tip can improve surface quality and geometric accuracy by reducing the undesired material flow due to local friction in the increment sheet forming process.

• 센서학회지
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• 제33권3호
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• pp.158-164
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• 2024

We developed a self-operated paper pump that can maintain a nearly constant flow rate of an aqueous solution along a paper strip channel in paper-based analytical devices (PADs). The quasi-stationary flow rate was controlled by increasing the crosssectional channel area (capillary force) using a fan-shaped absorption pad coupled with a paper strip channel. The flow rate is regulated by varying the fan angle of the circular absorbing pad. Furthermore, the flow rate can be increased by furnishing a hollow cavity at the center of a conventional paper strip channel. The rate was regulated by varying the length of the hollow paper channel in the flow rate range of 5.1-26.4 mm/min. As a preliminary work, a paper-pump-coupled PAD was fabricated, and its CV detection capability was evaluated for the redox reaction of Fe(CN)₆^+4/+3. The combination of a paper pump with a PAD resulted in an ideal CV curve with a higher limiting current and faster response time. These results are interpreted well by the Levich equation, which suggests that the paper pump is a very useful component in paper-based sensors.

• 한국산업융합학회 논문집
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• 제27권3호
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• pp.519-525
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• 2024

In this paper, a study on the physical properties of mortar applying 3D Textile was conducted to compensate for the shortcomings of the existing concrete surface repair and reinforcement method. In the tests conducted to analyze the physical properties, compressive strength, flexural strength, and dynamic modulus measurement tests were conducted. As a result of the compressive strength test, as the number of surfaces to which the stereoscopic fiber was applied increased, the amount of displacement and strength reduction rate increased, and the flexural strength also increased as the number of surfaces to which the stereoscopic fiber was applied increased. In addition, it was confirmed that the use of stereoscopic fibers tended to decrease the dynamic modulus of elasticity. This result is a characteristic of the application of stereoscopic fibers, and it caused a decrease in compressive strength due to a decrease in the mortar content of the part to which the stereoscopic fib er was applied, and the high tensile force of the stereoscopic fiber is believed to have affected the increase in flexural strength.

• 한국물환경학회지
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• 제24권6호
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• pp.718-724
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• 2008

The ion selective microelectrode (ISME) has been used for measuring the ion profile of DO, $NH_4{^+}-N$, $NO_2{^-}-N$ and $NO_3{^-}-N$ in biofilm. In this study we evaluated the detection limit and validity of ISME and applied ISME for the continuous measurement of $NH_4{^+}-N$ and $NO_3{^-}-N$ concentration in the modified Ludzack-Ettinger (MLE) process. Average detection limits of $NH_4{^+}-N$ and $NO_3{^-}-N$ ISME were $10^{-4.44}M$ and $10^{-4.62}M$, respectively. Since the ISME with $5{\sim}10{\mu}m$ of tip diameter showed a faster response time than that of $1{\sim}5{\mu}m$, the ISME with a tip diameter of $5{\sim}10{\mu}m$ was fabricated and used to make real-time ion detections. Direct monitoring of $NH_4{^+}-N$ and $NO_3{^-}-N$ concentrations in the aerobic (2) tank causes the instability of the electromotive force (EMF) for the initial 5~8 hours and also causes remarkable error values of $NH_4{^+}-N$ and $NO_3{^-}-N$ concentration. This phenomenon is caused by aeration and mixing in the reactor. Thus, the measuring chamber was newly designed for the aerobic (2) tank and then the EMF of the ISME were stabilized in less than 1 hour. Errors of $NH_4{^+}-N$ and $NO_3{^-}-N$ concentration were decreased after stabilization of the EMF. The ISME analysis were well corresponded to the results of auto analyzer and ion chromatography. Consequently, the concentration of $NH_4{^+}-N$ and $NO_3{^-}-N$ could be continuously measured for 178 hours by the ISME.

• 한국안광학회지
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• 제12권4호
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• pp.77-81
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• 2007

구면 렌즈는 굴절력에 따라 크게 (+)디옵터와 (-)디옵터 렌즈로 구분할 수 있다. 렌즈에 가해지는 외력에 의해 발생되는 변형은 디옵터의 증가 또는 감소에 따라 다르게 발생된다. 본 논문에서는 수년간 광계측 분야에서 널리 사용되고 있는 ESPI를 이용하여 렌즈에 발생되는 변형을 정량적으로 측정 하였다. ESPI(Electronic Speckle Pattern Interferometry: ESPI)는 빛의 가간섭성을 이용하여 대상물의 변형을 비접촉으로 정밀하게 측정할 수 있다는 장점을 지니고 있다. 실험은 총 16종의 플라스틱 안경 렌즈를 대상으로 수행 하였다. 동일한 변위를 주었을 때, (+)렌즈는 디옵터가 증가함에 따라 변형량이 감소하고, (-)렌즈의 경우 (+)디옵터 렌즈와는 반대로 디옵터가 감소함에 따라 변형량이 증가한다는 것을 확인 하였다. 또한 (+)디옵터 렌즈가 (-)디옵터 렌즈에 비해 상대적으로 변형량이 적게 발생한 사실을 알 수 있었다. 따라서 본 논문의 결과는 다양한 렌즈에 외부 변위가 가해지는 경우 렌즈의 변형에 의한 광학적 결함 등을 정량적으로 측정 할 수 있는 가능성을 제시하고 렌즈 산업 분야에 다양하게 응용 될 것으로 기대된다.

• 한국구조물진단유지관리공학회 논문집
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• 제18권3호
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• pp.84-92
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• 2014

본 연구에서는 PC교량의 외부텐던의 장력을 평가하기 위하여 신속하고 경제적으로 적용할 수 있는 텐던의 진동을 이용한 고유진동수 측정 방법의 적용성을 검토하였다. 길이별로 다양한 여러 종류의 외부텐던 덕트 표면에 가속도 센서를 부착하고 고유진동수를 측정하였다. 센서의 방향과 위치변경 등 여러 종류의 다양한 진동시험을 실시한 결과 고유진동수의 차이는 1% 이내로서 충분한 정확성을 확보하고 있음을 보였다. 측정된 고유진동수로부터 장력을 추정하기 위해서는 현의공식, 다중모드법, 저차 모드의 실용적 공식을 활용한 방법 (Zui et al., 1996), 양단고정 경계조건에 의한 휨강성을 고려한 현의공식 (Sag$\ddot{u}\grave{e}$s et al., 2006) 등의 장력 추정법을 적용하였고 그 결과값을 비교하였다. 휨강성을 반영하지 못한 현의 공식이나, 다중모드법은 길이가 짧은 텐던에 대해 장력을 과대평가하는 결과가 도출되어 텐던의 장력평가에 적절하지 않음을 알 수 있었다. 텐던의 장력 추정에 적절한 방법은 양단고정 경계조건과 휨강성을 고려한 단단한 현의 공식에 의한 방법임을 알 수 있었으며, 이 방법을 실제 공용중인 PC 교량의 외부텐던에 적용한 결과 방향전환블록에 의한 마찰력으로 긴장단과 고정단에 텐던의 장력 차이가 발생한다는 것을 알 수 있었다.