• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.203-206
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• 2003

New orchardgrass(Dactylis glomerata L.) variety named as “Jangbeol 101” was developed by the National Livestock Research Institute(NLRI) in 2002. To develope the new variety of orchardgras, 5 superior clones were selected and polycrossed for seed production. Synthetic seeds were examined on the agronomic growth characteristics and forage production at Suwon from 1995 to 1998, and regional trials were conducted in Suwon, Namwon and Pyungchang from 1999 to 2002 and Jeju from 2000 to 2002, respectively. “Jangbeol 101” showed semi-erect growth habit in fall and spring and medium type in length of flag leaf and upper internode. Plant height of “Jangbeol 101” was short compared to that of standard variety, “Ambassador” and heading date was delayed about 2 days as 14th May compared to Ambassador. Characters such as winter hardiness, regrowth, moisture tolerance, disease resistance of “Jangbeol 101” were stronger or better than those of Ambassador. “Jangbeol 101” showed 9% higher dry matter yield as 12,374 kg/ha compared to Amabassador. Nutritive value was appeared to be similar in both varieties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.207-210
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• 2003

"Jangbeol 102" is a new orchardgrass(Dacrylis glomerata L.) variety developed by the National Livestock Research Institute(NLRI) in 2002. To develope the new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. Agronomic growth characteristics and forage production of "Jangbeol 102" were examined at Suwon from 1995 to 1998, and regional trials were conducted in Suwon, Namwon and Pyungchang from 1999 to 2002 and Jeju from 2000 to 2002, respectively. "Jangbeol 102" showed semi-erect growth habit in fall and spring and medium to long type in length of flag leaf and upper internode. Plant height of "Jangbeol 102" was similar to that of standard variety, "Ambassador" and heading date was I day faster as 11th May compared to Ambassador. Characters such as winter hardiness, regrowth, moisture tolerance, disease resistance of "Jangbeol 102" were stronger or better than those of Ambassador, specially in regrowth and disease resistance. "Jangbeol 102" showed 18% higher dry matter yield(13,430kg/ha) compared to Amabassador. Nutritive value was appeared to be similar in both varieties.assador. Nutritive value was appeared to be similar in both varieties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.3
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• pp.165-170
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• 2008

Timothy (Phleum pratense L.) is an important grass species as forage. In order to optimize tissue culture conditions of timothy, the effects of plant growth regulators on callus induction and plant regeneration was investigated with mature seeds of colt cultivar. The optimal concentration of 2,4-D for the induction of primary callus from mature seeds was 3 mg/L. The highest embryogenic callus frequenc (25%) was observed when the mature seed were cultured on MS medium supplemented with 3 mg/L 2,4-D and 0.1 mg/L BA. The highest plant regeneration frequency was observed when type B callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of timothy by the production of transgenic plant.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.1-6
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• 2008

Korditwo is a new orchardgrass(Dactylis glomerata L.) variety developed by the National Institute of Animal Science(NIAS) in 2005. To develope the new variety of orchardgrass, 5 superior clones, Dg8533, Dg9518, EDg9133, U8504 and U8519, were selected and polycrossed for seed production. That synthetic variety was designated newly as "Korditwo", and it's agronomic growth characteristics and forage productivity were examined at Suwon from 2001 to 2002, and regional yield trials were conducted in Suwon, Pyungchang, Youngju, Iksan, and Jeju from 2003 to 2005, respectively. "Korditwo" showed medium type growth habit in fall and spring and short in length of flag leaf and upper internode. Plant height of "Korditwo" was similar to that of standard variety, "Ambassador" and heading date was 4 days later than 14th May compared to Ambassador. Characteristics such as winter hardiness, lodging tolerance, disease resistance of "Korditwo" were stronger or better than those of Ambassador, specially in winter hardiness and lodging tolerance. "Korditwo" showed 10% higher dry matter yield(11.3 ton/ha) compared to Amabassador. Nutritive value was appeared to be similar in both varieties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.6-9
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• 2013

"Onnuri" is a new orchardgrass (Dactylis glomerata L.) cultivar developed by the National Institute of Animal Science (NIAS) in 2011. To develop the new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. The agronomic growth characteristics and forage productivity of "Onnuri" were examined at Cheonan from 2009 to 2011, and regional trials were conducted in Cheonan, Pyeongchang, Jinju and Jeju from 2009 to 2011, respectively. "Onnuri" showed medium type growth habit in fall and spring, and medium in length of flag leaf and long upper internode. Plant height of "Onnuri" was 5cm more than that of the standard cultivar, "Amba" and the heading date was 5 days earlier than that of Amba (16th May). Characteristics, such as waterlogging and disease resistance, of "Onnuri" were stronger or better than those of Amba, Especially, dry matter yield of "Onnuri" (14,775 kg/ha) increased by 18% compared to that of Amba (12,523 kg/ha). Nutritive values appeared to be similar in both varieties.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.1
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• pp.15-18
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• 2020

'Luckyone 2ho' is a new orchardgrass (Dactylis glomerata L.) cultivar developed by the National Institue of Animal Science (NIAS) in 2017. To develope the new variety of orchardgrass, 5 superior clones were selected and polycrossed for seed production. The agronomic growth characteristics and forage productivity of 'Luckyone 2ho' were examined at Cheonan from 2012 to 2014, and regional trials were conducted in Cheonan, Pheonchang, Jinju and Jeju from 2015 to 2017, respectively. 'Luckyone 2ho' showed medium type growth habit in fall, and medium in length of flag leaf and very long upper internode. Plant height of 'Luckyone 2ho' was 3 cm less than that of standard cultivar, 'Potomac' and heading date was 1 days later than 5th May compared to standard cultivar, 'Potomac'. Characteristics such as waterlogging and disease resistance of 'Luckyone 2ho' were stronger or better than those of standard cultivar, 'Potomac', Especially, dry matter yield of 'Luckyone 2ho'(15,980 kg/ha) increased 9 % compared to that of standard cultivar, 'Potomac'(14,702 kg/ha). Nutritive values were appeared to be similar in both varieties except in Vitro dry matter digestibility(IVDMD) and crude protein and total digestible nutrients (TDN).

• Journal of The Korean Society of Grassland and Forage Science
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• v.4 no.3
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• pp.187-193
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• 1984

Using the stratifying clip method, we examine the differences in vertical distribution of leaf area and its relationships with dry matter production were investigated in the primary swards of six varieties. The results obtained were as follows: 1. The varieties could be classified into 4 different types according to their of vertical distribution of leaf area within the canopy. Thus, M had a greater leaf area distribution in the base layers and became smaller towards the upper layers, Leto, First and Bundy had the leaf area distribution which was most abundant in the middle layers and became smaller towards the base and the upper layers, Tammisto had a greater leaf area distribution in the middle layers and intensively distributed to the upper layers, and Trader had the relatively uniform leaf area distribution over all layers. 2. Effective leaf area index(ELAI) correlated with leaf area index(LAI), dry weight of plant(DW), stem weight of plant(SW) and stem area index(SAI). 3. The variety with the heading tiller type had the effective leaf area intensively distributed to the upper layers of canopy, but the vegetative type showed a high ratio of effective leaf area index. 4. Varietal differenced was found between the effective leaf area and specific leaf weight(SLW) in each stratum above the layer indicating the highest leaf area density within the canopy. M and Trader had a highly negative significant correlation, but the other varieties was not significant correlation.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.10
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• pp.1390-1394
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• 2004

We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. Transformation efficiency was influenced by the A. tumefaciens strain, addition of the phenolic compound acetosyringone and duration of vacuum treatment. Of the three A. tumefaciens strains tested, EHA101/pIG121Hm was found to be most effective followed by GV3101/pIG121Hm and LBA4404/pIG121Hm for transient GUS expression after 3 days co-cultivation. Inclusion of 100 $\mu$M acetosyringone in both the inoculation and co-cultivation media lead to an improvement in transient GUS expression observed in targeted calli. Vacuum treatment during infection of calli with A. tumefaciens strains increased transformation efficiency. The highest stable transformation efficiency of transgenic plants was obtained when mature seed-derived calli infected with A. tumefaciens EHA101/pIG121Hm in the presence of 100 $\mu$M acetosyringone and vacuum treatment for 30 min. Southern blot analysis indicated integration of the transgene into the genome of tall fescue. The transformation system developed in this study would be useful for Agrobacterium-mediated genetic transformation of tall fescue plants with genes of agronomic importance.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.307-312
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• 2009

This experiment was conducted to compare the agronomic characteristics and productivity of induced annual legumes at paddy field of Cheonan and Naju from 2004 to 2006. Chinese milk vetch (wild type), crimson clover (C.V. Linkarus), forage pea (C.V. Austrian pea), hairy vetch (C.V. Oregon commen) were used in this study. At the Naju region, the earliest flowering on 2nd may was found in Chinese milk vetch, while the latest on 18th May in forage pea. Fresh, dry matter (DM) and crude protein (CP) yields of crimson clover were highest as 28,870, 6,986 and 943 kg/ha, respectively. but the yield of chinese milk vetch was low by 9,885, 1,749 and 338 kg/ha. At the Cheonan region, the most fast flowering date variety was Chinese milk vetch, hairy vetch was very late as 19th May. Fresh, DM and CP yields of hairy vetch were highest as 7,916, 1,141 and 113 kg/ha, respectively. but the yield of chinese milk vetch showed the lowest by 270, 42 and 8 kg/ha. The CP content of annual legumes was 14.3%. chinese milk vetch showed the highest CP content by 19.3% and hairy vetch showed the lowest content by 9.9%. The present data showed that the productivity was closely dependent on winter hardness, in conclusion, crimson clover at Naju and hairy vetch at Cheonan region was suggested to be the most proper species as winter crop after rice harvest on paddy field when considered the winter hardiness.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.2
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• pp.79-88
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• 2022

This study was conducted to evaluate the productivity of whole crop silage wheat utilizing the paddy fields during a couple of years from 2019 to 2021 in Suwon, Korea. This investigation was used the two maturity types of wheat cultivar 'Cheongwoo' (early) and 'Taeu' (late). The heading date of 2^nd year (Oct. 2020 to May. 2021) cultivation was delayed about 11 to 13 days by more than 1^st year (Oct. 2019 to May. 2020). The growth characteristics were shown that the plant height was increased in 2^nd cultivation, while the number of culms and the panicle part ratio were decreased. Moreover, the nutritive value of 'Cheongwoo' and 'Taeu' were also decreased in 2^nd cultivation. These changes have thought to a difference of the precipitation by cultivation years. Because, the precipitation during the period from the end of winter dormancy to the harvesting stage in 2^nd (337 mm) cultivation was more about twice than 1st (169.3 mm) cultivation. However, the dry matter yield of 'Cheongwoo' was not shown a statistical difference by cultivation years, while 'Taeu' was shown to decrease tendency. The total dry matter yield regardless of the cultivation years were higher in 'Cheongwoo' than 'Taeu', and especially 'Cheongwoo' was more 3 tons per hectare (15.3 t/ha) than 'Taeu' (12.6 t/ha) at 2^nd cultivation (p<0.01). The trend of dry weight in 'Cheongwoo', early mature type, showed a relatively high ratio of dry matter (p<0.05) was considered that due to a high panicle ratio by a fast heading and an adequate weight of panicles by a sufficient maturing. In conclusion, selecting the early maturity cultivars could achieve a higher and more stable total dry matter yield considering the cropping system in the central region. Furthermore, it also has the advantage of being able to double-cropping system with forage rice, which has considered the maximum whole-crop forage production year-round. These results suggest that the 'Cheongwoo' be optimum cultivar to produce the year-round forage on paddy fields in the central region.