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http://dx.doi.org/10.5333/KGFS.2008.28.3.165

Callus Induction and Plant Regeneration from Mature Seeds of Timothy  

Lee, Ki-Won (National Institute of Animal Science, RDA)
Kim, Ki-Yong (National Institute of Animal Science, RDA)
Choi, Gi-Jun (National Institute of Animal Science, RDA)
Lim, Young-Chul (National Institute of Animal Science, RDA)
Kim, Won-Ho (National Institute of Animal Science, RDA)
Jung, Min-Wong (National Institute of Animal Science, RDA)
Seo, Sung (National Institute of Animal Science, RDA)
Lee, Byung-Hyun (Division of Applied Life Science (BK21 program), Gyeongsang National University)
Lee, Sang-Hoon (National Institute of Animal Science, RDA)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.28, no.3, 2008 , pp. 165-170 More about this Journal
Abstract
Timothy (Phleum pratense L.) is an important grass species as forage. In order to optimize tissue culture conditions of timothy, the effects of plant growth regulators on callus induction and plant regeneration was investigated with mature seeds of colt cultivar. The optimal concentration of 2,4-D for the induction of primary callus from mature seeds was 3 mg/L. The highest embryogenic callus frequenc (25%) was observed when the mature seed were cultured on MS medium supplemented with 3 mg/L 2,4-D and 0.1 mg/L BA. The highest plant regeneration frequency was observed when type B callus was transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Regenerated plants were grown normally when shoots were transplanted to the soil. A short tissue culture period and regeneration system would be beneficial for molecular breeding of timothy by the production of transgenic plant.
Keywords
Timothy; Callus; Plant regeneration; Tissue culture;
Citations & Related Records
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1 Miki, K., T. Koichi, C.H, Bae and H.Y. Lee. 2003. Plant regeneration and transformation of kentucky bluegrass (Poa pratensis L.) via the plant tissue culture. Korea J. plant biotech. 30:115-121   과학기술학회마을   DOI   ScienceOn
2 Montague, A., A. Ziauddin, R. Lee, W.M. Ainley and J. Strommer. 2008. High-efficiency phos- phinothricin-based selection for alfalfa. trans- formation. Plant Cell Tissue Organ Culture. DOI 10.1007/s11240-007-9274-8
3 Lee, S.-H., D.-G. Lee, H.-S. Woo, K.-W. Lee, D.-H. Kim, S.-S. Kwak, J.-S. Kim, H. Kim, N. Ahsan, M.S. Choi, J.-K. Yang and B-H Lee. 2006. Production of transgenic orchardgrass via Agrobacterium-mediated transformation of seed- derived callus tissues. Plant Science, 171:408- 414   DOI   ScienceOn
4 Lee, S.-H., N. Ahsan, K.-W. Lee, D.-H. Kim, D.-G. Lee, S.-S. Kwak, S.-Y. Kwon, T.H. Kim and B.-H. Lee. 2007. Simultaneous overexpression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol. 164:1626-1638   DOI   ScienceOn
5 Lee, S.-H., D.-G. Lee, H.-S. Woo and B.-H. Lee. 2004. Development of transgenic tall fescue plants from mature seed-derived callus via Agrobacteriummediated transformation. Asian-Aust. J.Anim. Sci. 17:1390- 1394
6 Guo, Y.D. and S. Pulli. 2001. Somatic embryogenesis and plant regeneration from suspension cultures of timothy (Phleum pratense L.). Act Agr Scand B-S P. 51: 160-166
7 Plazek, A., M. Filek and M. Wfdzony. 1999. Improvement of regeneration ability in Phleum pratense L. in vitro culture by dicamba. Acta Physiologiae Plantarum. 21:397-403   DOI   ScienceOn
8 Chen, L., C.K. Auh, P. Dowling, J. Bell, F. Chen, A. Hopkins, R. A. Dixon and Z.Y. Wang. 2003. Improved forage digestibility of tall fescue (Festuca arundinacea) by transgenic down- regulation of cinnamyl alcohol dehydrogenase. Plant Biotechnol. J. 1:437-449   DOI   ScienceOn
9 Zhong, H. and M. B. Sticklen. 1991. Plant regeneration via somatic embryogenesis in creeping bentgrass. Plant Cell Report. 10:453- 456   DOI
10 Weeks, J.T., J. Ye and C.M. Rommens. 2008. Development of an in planta method for transformation of alfalfa. Transgenic Research. 10.1007/s11248-007-9132-9
11 Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-668
12 Dong, S. and R. Qu. 2005. High efficiency transformation of tall fescue with Agrobacterium tumefaciens. Plant Science 168:1453-1458   DOI   ScienceOn
13 Bettany, A.J.E., S.J. Dalton, E. Timms, B. Manderyck, M.S. Dhanoa and P. Morris. 2003. Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.). Plant Cell Rep. 21:437-444   DOI
14 Bregitzer P. 1992. Plant regeneration and callus in barley: effects of geno and culture medium. Crop Sci. 32:1108-1112   DOI
15 Lee, K.-W., S.-H. Lee, D.-H. Kim, D.-G. Lee, S.-H. Won, H. Lee and B.-H. Lee. 2006. Effect of callus and antioxidants on plant regeneration and transformation of tall fescue. Journal of the korean society of grassland science. 26:77-82   과학기술학회마을   DOI   ScienceOn
16 Carvalho, C.H.S., N. Bohorova, P.N. Bordallo, L. L. Abreu, F.H. Valicente, W. Bressan and E. Paiva. 1997. Type II callus production and plant regeneration in tropical maize genotypes. Plant Cell Rep. 17:73-76   DOI
17 Chai, B.F., A.H. Liang, W. Wang and W. Hu. 2003. Agrobacterium-mediated transformation of kentucky bluegrass. Acta Bot. Sinica. 45: 966-973
18 Chaudhury, A. and Q. Rongda. 2000. Somatic embryogenesis and plant regeneration of turf- Bermuda grass: effect of 6-benzyladenine in callus induction medium. Plant Cell. Tiss Org. Cult. 60: 113-120   DOI
19 Griffin, J.D. and M.S. Dibble. 1995. High frequency plant regeneration from seed-derived callus cultures of Kentucky bluegrass (Poa pratensis L.). Plant Cell Report. 14:721-724
20 Hisano, H, A. Kanazawa, A. Kawakami, M. Yoshida, Y. Shimamoto and T. Yamada. 2004. Transgenic perennial ryegrass plants expressing wheat fructosyltransferase genes accumulate increased amounts of fructan and acquire increased tolerance on a cellular level to freezing. Plant Science. 167:861-868   DOI   ScienceOn
21 Murashige, T. and Skoog, F. 1962. A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15:473-497   DOI
22 Sato, H. and T. Takamizo. 2006. Agrobacterium tumefaciens-mediated transformation of forage- perennial ryegrass (Lolium perenne L.). Grassland Science. 52:95-98   DOI   ScienceOn
23 Wu, Y.-Y., Q.-J. Chen, M. Chen, J. Chen and X.-C. Wang. 2005. Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar Na+/H+ antiporter gene. Plant Science. 169: 165-173   DOI   ScienceOn