• Title/Summary/Keyword: Forage characteristics

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A New Whole Crop Barley Cultivar 'Younghan' with Good Quality, Resistance to Lodging and Viral Disease and High-Yielding (양질 내병 내도복 다수성 청보리 품종 '영한')

  • Park, Tae-Il;Seo, Jae-Hwan;Han, Ouk-Kyu;Kim, Kyeong-Hun;Oh, Young-Jin;Park, Ki-Hun;Choi, Jae-Seong;Park, Jong-Chul;Park, Hyoung-Ho;Kim, Hong-Sik;Kim, Jung-Gon;Song, Tae-Hwa;Kim, Won-Ho;Park, Nam-Geon;Jeung, Jae-Hyun;Ju, Jung-Il;Kim, Soo-Yong;Kim, Dae-Ho
    • Korean Journal of Breeding Science
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    • v.43 no.3
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    • pp.184-189
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    • 2011
  • 'Younghan' (Hordeum vulgare L.), a winter barley for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration in 2008. It was derived from a cross between $F_1$['YB3433-3B-5'/'YB3135-3B-2-3'] and 'YB3135-3B-2-3'. 'SB971305-B-B-B-4-4' line was selected for its earliness, resistance to disease and good agronomic characteristics. The promising line showed both high yield and lodging resistance in the yield trials in Iksan in 2004 to 2005, and designated as 'Iksan420'. The line was subsequently evaluated for winter hardiness, earliness, and yield at eight locations throughout Korea for two years from 2007 to 2008 and finally named as 'Younghan'. It has the growth habit of IV, erect plant type, green leaf and thick culm in diameter. Its heading date was April 24, and the maturing date was May 25 in paddy field conditions, which were one day earlier than those of the check cultivar 'Youngyang'. The cultivar 'Younghan' had better winter hardiness, and resistance to lodging and BaYMV than those of the check cultivar did. The average forage dry matter yield of 'Younghan' was approximately 12.0 MT/ha in adapted region. 'Younghan' also showed 8.5% of crude protein content, 27.2% of ADF, and 67.1% of TDN, including higher grade of silage quality for whole crop barley. This cultivar would be suitable for (area with daily minimum temperature in January) above $-8^{\circ}C$ in Korean peninsula.

Effect of the Sowing and Harvesting Dates on the Agronomic Characteristics and Feed Value of Corn and Sorghum×Sorghum Hybrid in Youngnam Mountain Area (영남산간지역에서 옥수수와 수수×수수 교잡종의 파종시기와 수확시기가 조사료 생육 특성과 사료가치에 미치는 영향)

  • Lee, Hyuk-Jun;Joo, Young-Ho;Lee, Seong-Shin;Paradipta, Dimas Hand Vidya;Han, Ouk-Kyu;Ku, Ja-Hwan;Min, Hyeong-Gyu;Oh, Jung-Sik;Kim, Sam-Churl
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.2
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    • pp.53-60
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    • 2019
  • This study was carried out from 2015 to 2016 to identify the suitable sowing and harvesting dates of summer crops in the mountain of Yeongnam, South Korea. The experimental design consisted of the different sowing and harvesting dates as follows: corn hybrid (Z. mays, Kwangpyeongok) of sowing (May 8, 19, and 27) and harvesting (August 10, 20, and 30); sorghum${\times}$sorghum hybrid (Sorghum bicolor ${\times}$ Sorghum bicolor, SS405) of sowing (May 27, June 19 and June 27) and harvesting (August 10, 20, and 30). In corn hybrid, ear rate and dry matter (DM) yield decreased (p<0.05) with the postponement of sowing date. Otherwise, ear rate and DM yield increased (p<0.05) with the postponement of harvesting date. Crude protein content decreased (p<0.05) with the postponement of sowing date, but neutral detergent fiber content increased (p<0.05). In sorghum${\times}$sorghum hybrid, plant length and DM yield with the postponement of harvesting date increased (p<0.05), while crude protein content with the postponement of harvesting date decreased (p<0.05). This study concluded that sowing corn hybrid in early May and sorghum${\times}$sorghum hybrid in early June then harvest in the middle of August was recommend to increase dry matter yield and feed value.

Effects of Mixed Application of Chemical Fertilizer and Liquid Swine Manure on Dry Matter Yield and Feed Value of Whole Crop Barley (화학비료와 발효 돈분 액비 혼용 시용이 총체보리의 생산성 및 영양성분에 미치는 영향)

  • Sang Moo Lee
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.225-231
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    • 2023
  • This study was carried out to investigate the growth characteristics, yield and chemical compositions of whole crop barley (Hordeum vulgare L.) according to mixing ratio of chemical fertilizer (CF) and liquid swine manure (LSM) in the paddy field cultivation. The experimental design was arranged in a randomized block design with five treatments and three replications. The manure fertilizer ratio of five treatments were CF 100% (T1), CF 70% + LSM 30% (T2), CF 50% + LSM 50% (T3), CF 30% + LSM 70% (T4), and LSM 100% (T5) of whole crop barley. At this time, the application of liquid swine manure was based solely on the nitrogen. Plant length was higher at T1 as compared to other treatments (T2, T3, T4 and T5). Fresh yield, dry matter yield and total digestive nutrients (TDN) yield were the highest in T1, whereas the lowest in T5 treatment (p<0.05). Chemical compositions (crude protein, crude fat, neutral detergent fiber, acid detergent fiber and TDN) did not show significant difference among treatments. Ca and Na contents were significantly lower at T1 as compared to other treatments (T2, T3, T4 and T5). However, Mg and P contents were significantly higher at T1 as compared to other treatments(p<0.05). There was no significant difference in total free sugar content among T2, T3, T4 and T5 treatments, but the chemical fertilizer (T1) was significantly lower than the other treatments (p<0.01). Considering the above results, liquid swine manure application showed lower dry matter yield and TDN yield than chemical fertilizer, but higher free sugar content. Therefore, in order to increase the productivity of whole crop barley, it is considered desirable to mix liquid fertilizer with chemical fertilizer, taking into account the decomposition rate and insufficient components (P, K) of the liquid swine manure.

Effect of Protein Fractionation and Buffer Solubility of Forage Sources on In Vitro Fermentation Characteristics, Degradability and Gas Production (조사료 자원의 단백질 분획 및 Buffer 추출이 In Vitro 발효 성상, 분해율 및 Gas 생성량에 미치는 효과)

  • Jin, Guang Lin;Shinekhuu, Judder;Qin, Wei-Ze;Kim, Jong-Kyu;Ju, Jong-Kwan;Suh, Seong-Won;Song, Man-Kang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.32 no.1
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    • pp.59-74
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    • 2012
  • Buffer solubility and protein fractionation were evaluated from the hays (timothy, alfalfa and klein) and straws (tall fescue and rice), and $In$ $vitro$ trial was conducted to examine the effect of buffer extraction on fermentation characteristics, degradability and gas ($CO_2$ and $CH_4$) production. Buffer soluble protein (SP) content and A fraction in total protein were highest in alfalfa hay as 61% and 41.77%, respectively while lowest in rice straw (42.8% and 19.78%, respectively). No difference was observed in B1 fraction among forages but B2 fraction was slightly increased in klein hay (12.34%) and tall fescue straw (10.05%) compared with other forages (6.34~8.85%). B3 fraction of tall fescue was highest as 38.49% without difference among other forages while C fraction was highest in rice straw. pH in incubation solution was higher in all forages after extraction than before extraction at 3h (P<0.01) and 6h (P<0.05), and pH from hays of timothy and alfalfa was higher than the other forages at 6h (P<0.05) and 12h (P<0.001). Regardless of extraction, ammonia-N concentration from alfalfa hay was increased at all incubation times and extraction effect was appeared only at 3h incubation time (P<0.01). Total VFA concentration from alfalfa hay was highest up to 24h incubation while those from tall fescue straw and rice straw were lowest. Buffer extraction decreased (P<0.01~P<0.001) the total VFA concentration. Acetic acid proportion was increased (P<0.001) before extraction of forages but no difference was found between forages. Propionic acid($C_3$) proportion was also increased(P<0.001) before extraction in all forages than in straws at 3h, 24h and 48h incubations, and $C_3$ from hays were mostly higher (P<0.05) than from straws. Butyric acid proportion, however, was not affected by extraction at most incubation times. Parameter 'a' regarding to the dry matter (DM) degradation was increase (P<0.001) in all forages before extraction, and was decreased (P<0.05) in tall fescue straw and rice straw compared with hays. Parameter 'b' was also increased (P<0.001) before extraction but no difference was found between forages. Effective degradability of DM (EDDM) was higher (P<0.001) before extraction in most forages except for rice straw. Buffer extraction decreased (P<0.05) all parameters (a, b, and c) regrading to the crude protein (CP) degradation but no difference was found between forages. Effective degradation of CP (EDCP) was lower (P<0.05) in straws than in hays. Parameters 'a' and 'b' regarding to the NDF degradation (P<0.01) and effective degradability of NDF (EDNDF, P<0.001) were also higher in forages before extraction than after extraction but no difference was found between forages. Buffer extraction reduced (P<0.05~P<0.001) $CO_2$ production from all the forages uo to 24h incubation and its production was greater (P<0.05~P<0.01) from hays than straws. Methane ($CH_4$) production was also greater (P<0.01~P<0.001) in all forages at all incubation times, and its production was greater (P<0.05) from hays than from straws at most incubation times. Based on the results of the current study, it can be concluded that buffer solubility and CP fractionation might be closely related with $In$ $vitro$ VFA concentration, degradability and gas ($CO_2$ and $CH_4$) production. Thus, measurement of buffer solubility and protein fractionation of forages might be useful to improve TMR availability in the ruminants.

Effect of Plowing Frequency and Sowing Dates on the Agronomic Characteristics, Feed Value, Weed Yield and Palatability of Silage Corn (경운횟수와 파종기 이동이 사일리지용 옥수수의 생육특성, 사료가치, 잡초발생 및 가축의 기호성에 미치는 영향)

  • Lee, Sang-Moo;Kim, Byoung-Tae;Hwang, Joo-Hwan;Jeon, Byoung-Tae;Moon, Sang-Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.3
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    • pp.209-218
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    • 2007
  • This experiment was conducted to investigate effect of plowing frequency and sowing dates on the agronomic characteristics, feed value, weed yield and palatability of silage corn. Treatments were a basal treatment(C: May 5 seeding, plowing once, weeding control once), T1(May 12 seeding, plowing twice, weeding control 0 time), T2(May 19 seeding, plowing three times, weeding control 0 time, T3(May 26 seeding, plowing four times, weeding control 0 time) and T4(June 2 seeding, plowing five times, weeding control 0 time). The experiment was performed at the College of Life and Natural Sciences of Sangju University in Sangju in 2006. The plant height and ear height showed highly in order to C > T1 > T2 > T3 > T4 treatment, leaf length was the highest at T2 (96.0cm). Leaf width and number of dead leaf were the highest at C and T3 (11.2cm), C, C and T1 (4.6), respectively. Stem diameter was the highest at T3 as 31mm, while T2 was the lowest as 25mm (p<0.05). Ear circle showed highly in order of C > T1 > T4 > T3 > T4 (p<0.05), and tip filling degree was the highest at C treatment as 8.8, while T4 treatment was the lowest as 6.0 (p<0.05). The stem hardness and grain hardness were C < T1 < T2 < T3 < T4 (p<0.05). Stem saccharinity was T1(6.1%) was the highest, while T2(3.0%) was the lowest(P<0.05). Fresh yield of weed was the lowest at C treatment as 500 kg/ha, but T1 treatment was the highest as 44,100 kg/ha (p<0.05). Weed coverage rate showed highly in order of T1 > T2 > T3 > T4 > C treatment (p<0.05). Fresh yield of corn was the highest at C treatment as T3,550 kg/ha, but T4 treatment was the lowest as 65,500 kg/ha (p<0.05). Dry matter yield of corn showed highly in order of C(26,978 kg/ha) > T1(26,130 kg/ha) > T2(20,255 kg/ha) > T3(20,255 kg/ha) > T4(17,508 kg/ha) treatment (p<0.05). Crude protein content was T1(7.69%) > T4(7.42%) > T2(6.34%) > T3(5.99%) > C(5.91%) treatment (p<0.05), and Crude fat content showed highly in order of C (2.13%) > T1(2.04%) > T2(1.96%) > T3(1.95%) > T4(1.84%) treatment. Relative palatability of Holstein, Korean native goat and spotted deer was the highest at C treatment, but Korean native cattle was the highest at T1 treatment.

Estimation of Availability of Whole Crop Barley and Rye Silage TMR in the Cattle (청보리 및 호밀 Silage를 이용한 거세한우 비육중기용 TMR의 소 체내 이용성 조사)

  • Qin, Wei-Ze;Jin, Guang Lin;Kim, Jong-Kyu;Oh, Young-Kyoon;Lee, Sang-Cheol;Song, Man-Kang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.30 no.4
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    • pp.343-354
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    • 2010
  • The present study was conducted to examine the fermentation characteristics and effective degradability (ED) in the rumen, and whole tract digestibility of whole crop silage based TMR in comparison with conventional separate feeding of concentrate and roughage. Three ruminally fistulated non-lactating Holstein cattle were used in a 3 $\times$ 3 Latin square design. The cattle were fed 8kg of whole crop barley silage based TMR (BS-TMR) or 8kg (DM basis) of whole crop rye silage based TMR (RS-TMR) twice (08:00 and 18:00) daily in an equal amount. The cattle were also fed concentrate (5.6kg) and rice straw (1.4kg) seperately (DM basis, Control) twice daily in an equal amount. The both silages were included in TMR at 20% level (as fed basis). pH in the rumen fluid was not influenced by the diets but was slightly higher from TMR than from control. No difference was found in ammonia-N concentration between diets. Total VFA concentration was relatively increased in the cattle fed RS-TMR to the other diets up to 6h post feeding. The proportion of acetate was increased in the TMR feeding at right before feeding (0 h, p<0.005) and 9 h (p<0.048) post feeding compared with control. Propionate proportion was increased (p<0.046) in both TMRs while butyrate proportion was increased (p<0.029) at 1h post feeding compared to other diets. Effective degradability (ED) of DM and CP of RS-TMR was relatively increased to other diets, and EDNDF of both TMRs was higher than that of control diet due to the increased parameters b (p<0.039) and c (p<0.006) in TMR treatments. Whole track digestibility of most components in the TMRs was slightly increased compared to that in control diet, and RS-TMR had a tendency to be increased whole track digestibility except for NDF compared to BS-TMR. Based on the results observed from the present study, nutrient availability of whole crop silage based TMR looked slightly better than conventional separate feeding of concentrate and rice straw, mainly due to the improved stabilization of fermentation in the rumen and increased NDF digestibility of whole crop silage in TMR.

Effects of Partial Replacement of Corn Grain and Soybean Meal with Agricultural By-Product Feeds on In Vitro Rumen Fermentation Characteristics and Optimum Levels of Mixing Ratio (농산부산물을 이용한 In Vitro 반추위발효 특성 및 적정 배합수준을 통한 옥수수 및 대두박 대체 효과)

  • Park, Joong-Kook;Lim, Dong-Hyun;Kim, Sang-Bum;Ki, Kwang-Seok;Lee, Hyun-June;Kwon, Eung-Gi;Cho, Won-Mo;Kim, Chang-Hyun
    • Journal of Animal Science and Technology
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    • v.53 no.5
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    • pp.441-450
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    • 2011
  • This study was conducted to determine the effects of partial replacement of corn grain and soybean meal with agricultural by-product feeds on in vitro rumen fermentation characteristics and optimum levels of mixing ratio. The agricultural by-products to examine the effectiveness of the partial replacement of concentrate were wheat bran, corn gluten feed, bakery waste, soybean curd, rice bran, green kernel rice, soybean hull, distillers' grain, and mushroom substrate. In the first experiment, in vitro ruminal fermentation characteristics of feedstuffs were evaluated at 0, 3, 6, 12, 24, and 48 hours after incubation. In the second experiment, fermentation characteristics were investigated with green kernel rice and soybean curd which replaced corn grain or soybean meal. Feed were formulated with 40% corn grain + 20% soybean meal (T1), 40% corn grain + 17.5% soybean meal + 2.5% soybean curd (T2), 25% corn grain + 20% soybean meal + 15% green kernel rice (T3), and 30% corn grain + 15% soybean meal + 6% green kernel rice + 9% soybean curd (T4), respectively, with forage source of 10% alfalfa hay, 20% timothy hay, and 10% corn silage as fed-basis. In 24 and 48 hour cultivations, T4 showed significantly lower pH compared to T1, whereas in 3 and 24 hour cultivations, T4 showed significantly higher DM degradation compared to T1. In addition, the gas production of T3 was also higher than T1 (p<0.05). Overall results of the present experiments indicated that green kernel rice and soybean curd as agricultural by-products have the possibility of partial replacements of corn grain and soybean meal.

Ecological Characteristics of Termite(Reticulitermes speratus kyushuensis) for Preservation of Wooden Cultural Heritage (목조문화재의 보존을 위한 한국산 흰개미의 생태적 특성 연구)

  • Lee, Kyu-Shik;Jeong, So-Young
    • Korean Journal of Heritage: History & Science
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    • v.37
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    • pp.327-348
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    • 2004
  • In this study, after analyzing several local climate characteristics of South Korea, I validated distribution, invasion, foraging, underground activities, attack season as ecological characteristics and also temperature, relative humidity, and tree species as preference characteristics of Korean termites (Reticulitermes speratus kyushuensis Morimoto). Especially, southern part of the Korean peninsula is a suitable area for inhabitation and motion of termites holding same ecological characteristic like R. speratus kyushuensis. Busan is a neighboring district at field distribution north limiting temperature of Coptotermes formosanus Shiraki and Chuncheon is a passing area through the Korean Peninsula of field distribution north limiting temperature of Reticulitermes speratus Kolbe. The termite attack of wood devices was about 34.5% for 3 years in the forest of Jongmyo. Although the attack rate of termite increased each year, the detection rate decreased and the missing rate was high by degrees. I confirmed a foraging habits which is a part of termite colony was a role of continuous decomposition and another was a role of new food hunt as experimental results. The foraging termites were found under ground at Jongmyo in Seoul from April to November in the 2001 and the most active period was on July and August. The termite invasion rate of bait station increased in every monitoring. Through the increasing attack rate of bait station during 2nd monitoring (November, 2000) and 3rd monitoring(March, 2001), I confirmed that termites moved into the deep underground in winter, and were working continuously to forage. R. speratus kyushuensis inhabiting at the Korean Peninsula is a species which has food consumption rate with higher temperature. The termite revealed the greatest amount of food(filter paper) at $30^{\circ}C$(90% RH), but showed increasing death rate at over $32^{\circ}C$. Also, survival rate of this termite was 97% at 84% RH($30^{\circ}C$), but killed 100% at 52% RH($30^{\circ}C$) and 70% RH($30^{\circ}C$). For wood feeding, this was observed the preference in a pine tree(Pinus densiflora) above all others. Survival of termites was high(87%) at a pine tree, but low(13.5%) at a paulownia tree(Paulownia coreana). In this study, I presented the biological characteristic of termite(R. speratus kyushuensis Morimoto) and confirmed the deterioration degree of termite on wooden cultural heritage in Korea. Depending on climate and soil temperature, each area in the southern part of the Korea Peninsula, has some different active period and different distribution of R. speratus kyushensis. With these results, I expect that this report helps to prepare the integrated pest management(IPM) of the termite on wooden cultural heritage in Korea, and it may help to reduce the economical loss from termite damage in Korea.

Nutritive Evaluation of Some Browse Tree Legume Foliages Native to Semi-arid Areas in Western Tanzania

  • Rubanza, C.D.K.;Shem, M.N.;Otsyina, R.;Ichinohe, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1429-1437
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    • 2003
  • Browse tree legume leaves from Acacia spp (A. nilotica, A. tortilis, A. polyacantha), Dichrostachys sp, Flagea villosa, Piliostigma thonningii, Harrisonia sp were evaluated for nutritive potential (chemical compositions and degradability characteristics) compared to Gliricidia sepium. Effect of tannins anti-nutritive activity on digestibility was also assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) differed (p<0.05) between legume foliages. Mean CP, ash, NDF, ADF and ADL for fodder species tested were 158, 92, 385, 145, and 100 g/kg DM, respectively. CP ranged from 115 (P. thonningii) to 205 g/kg DM (G. sepium). Acacia spp had moderate CP values (g/kg DM) of 144 (A. nilotica), to high CP in A. tortilis (188) and A. polyacantha (194) comparable to G. sepium. The forages had relatively lower fiber compositions. A. nilotica had (p<0.05) lowest NDF, ADF and ADL (182, 68 and 44) compared to P. thonningii (619, 196 and 130) g/kg DM, respectively. Except G. sepium, all fodder species had detectable high phenolic and tannin contents greater than 5% DM, an upper beneficial level in animal feeding and nutrition. Mean total phenolics (TP), total tannins (TT) and condensed tannins (CT) (or proanthocyanidins) for fodder species tested were 139, 113 and 43 mg/g DM, respectively. F. villosa had (p<0.05) lowest TP and TT of 65 and 56 mg/g DM, respectively, compared to A. nilotica (237 and 236 mg/g DM, respectively). The CT varied (p<0.05) from 6 (F. villosa) to 74 mg/g DM (Dichrostachys sp). In vitro organic matter (OM) degradability (OMD) differed (p<0.05) between fodder species. G. sepium had (p<0.05) high degradability potential compared to A. polyacantha that had (p<0.05) the lowest OMD values. Forage degradability ranked: G. sepium>A. nilotica>P. thonningi>F. villosa>Dichrostachys sp>A. tortilis>A. polyacantha. Addition of PEG resulted to (p<0.05) improvement in in vitro OM digestibility (IVD). Increase in IVD was mainly due to binding action of PEG on tannins; and represents potential nutritive values previously depressed by tannins anti-nutritive activity. Browse fodder has potential as sources of ruminal nitrogen especially for ruminants consuming low quality roughages due to high protein, lower fiber compositions and high potential digestibility. However, utilization of browse supplements in ruminants is hampered by high phenolic and tannin contents. Deactivation of tannin anti-nutritive activity, possibly by feeding tanniniferous browse with other readily available nitrogen sources to dilute tannin anti-nutritive activity could improve utilization of browse fodder supplements. Further studies are needed to assess browse fodder palatability and intake, and their effect on growth performance in ruminants.

Taxonomic Revision of Variation in Skull Morphology of Siberian Roe Deer (Capreolus pygargus, Pallas, 1771) in South Korea (머리뼈 형태학적 특성을 이용한 한국산 노루의 분류학적 고찰)

  • Park, Yong-Su;Cha, Jin-Yeol;Kim, Nam-Shin
    • Korean Journal of Environment and Ecology
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    • v.30 no.1
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    • pp.39-47
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    • 2016
  • Recently, it has been reported that morphologically and genetically different two Siberian roe deer (Capreolus pygargus, Pallas, 1771), in Mainland and in Jeju island. Until now, this roe deer has not been clearly taxonomy of Siberian roe deer in Korean peninsula. So, the aim of this study was to compare the skull morphometrics of mainland and Jeju roe deer to obtain more information on the species status of C. pygargus and study the distribution of roe deer subspecies in this region. A total of 50 skulls of adult roe deer was compared and analyzed. The maximum skull length, condylo-basal length, basilar length, maximum skull width, cheek-bone width, interorbital width, rostrum length, length of upper tooth row from the Siberian roe deer in Jeju island were significantly smaller than those of the deer in mainland. Length of the skull was the principal factor of interpopulational variability in roe deer. Variation was not distributed along latitude, longitude, or elevation, but those factors did influence the variation through effects on primary production, which in turn caused isolated differences in skull morphology apparently related to types of forage selected by roe deer in various geographic areas. According to the analysis results of the skull characters, Mainland roe deer was more similar to C. p. tianschanicus which is a subspecies of Siberian roe deer. In addition, Jeju roe deer showed distinct differences compared to European roe deer, 3 subspecies of Siberian roe deer, so it appeared that they could be a unique native species inhabited only in Jeju Island. Because Siberian roe deer in Jeju Island can be classified at subspecies level in this study.