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Effects of Partial Replacement of Corn Grain and Soybean Meal with Agricultural By-Product Feeds on In Vitro Rumen Fermentation Characteristics and Optimum Levels of Mixing Ratio

농산부산물을 이용한 In Vitro 반추위발효 특성 및 적정 배합수준을 통한 옥수수 및 대두박 대체 효과

  • 박중국 (농촌진흥청 국립축산과학원) ;
  • 임동현 (농촌진흥청 국립축산과학원) ;
  • 김상범 (농촌진흥청 국립축산과학원) ;
  • 기광석 (농촌진흥청 국립축산과학원) ;
  • 이현준 (농촌진흥청 국립축산과학원) ;
  • 권응기 (농촌진흥청 국립축산과학원) ;
  • 조원모 (농촌진흥청 국립축산과학원) ;
  • 김창현 (한경대학교 동물생명환경과학부)
  • Received : 2011.05.27
  • Accepted : 2011.10.21
  • Published : 2011.10.31

Abstract

This study was conducted to determine the effects of partial replacement of corn grain and soybean meal with agricultural by-product feeds on in vitro rumen fermentation characteristics and optimum levels of mixing ratio. The agricultural by-products to examine the effectiveness of the partial replacement of concentrate were wheat bran, corn gluten feed, bakery waste, soybean curd, rice bran, green kernel rice, soybean hull, distillers' grain, and mushroom substrate. In the first experiment, in vitro ruminal fermentation characteristics of feedstuffs were evaluated at 0, 3, 6, 12, 24, and 48 hours after incubation. In the second experiment, fermentation characteristics were investigated with green kernel rice and soybean curd which replaced corn grain or soybean meal. Feed were formulated with 40% corn grain + 20% soybean meal (T1), 40% corn grain + 17.5% soybean meal + 2.5% soybean curd (T2), 25% corn grain + 20% soybean meal + 15% green kernel rice (T3), and 30% corn grain + 15% soybean meal + 6% green kernel rice + 9% soybean curd (T4), respectively, with forage source of 10% alfalfa hay, 20% timothy hay, and 10% corn silage as fed-basis. In 24 and 48 hour cultivations, T4 showed significantly lower pH compared to T1, whereas in 3 and 24 hour cultivations, T4 showed significantly higher DM degradation compared to T1. In addition, the gas production of T3 was also higher than T1 (p<0.05). Overall results of the present experiments indicated that green kernel rice and soybean curd as agricultural by-products have the possibility of partial replacements of corn grain and soybean meal.

본 연구는 국내 농산부산물을 이용하여 in vitro 반추위 발효 특성을 조사하고 에너지 사료인 농후사료와 유사한 반추위 발효특성을 나타내는 농산부산물을 선정하여 최적배합수준을 통한 농후사료 대체 효과를 규명하기 위하여 수행하였다. 본 연구를 수행하기 위하여 국내에서 다량 생산되는 9점의 부산물(소맥피, 단백피, 제빵 부산물, 비지, 미강, 청치, 대두피, 주정박, 버섯배지)을 선발하였다. 1차 실험에서는 in vitro 배양을 통해 각각 원료사료의 시간대별(0, 3, 6, 12, 24, 48 h) 반추위 발효특성을 평가하였으며, 옥수수 및 대두박과 유사한 발효특성을 나타내는 원료사료 2종류를 선발 하였다. 최종적으로 선발된 2종류의 원료사료는 다른 원료사료와 혼합한 조합구를 만들어 in vitro 배양을 통한 옥수수와 대두박의 대체효과를 평가하였다. 실험설계는 옥수수와 대두박을 기본배합으로 하여, 알팔파, 티머시 건초 및 옥수수사일리지를 조사료로 사용하였다(T1). T2구는 대두박을 비지로 2.5% 대체, T3구는 옥수수를 청치로 15% 대체하였으며, T4구는 옥수수와 대두박을 청치와 비지로 각각 10 및 5% 대체하여 in vitro 반추위 발효특성을 평가하였다. 각 원료사료와 농산부산물의 in vitro 반추위 발효 특성을 조사한 결과, 옥수수 및 대두박과 유사한 발효특성을 나타내는 농산부산물로 청치와 비지를 선정하였다. 2차 실험에서는 pH의 경우, T4구에서 T1구와 비교하여 유의하게 낮았으며, 가스발생량은 T3 구에서 T1구와 비교하여 유의하게 높았다(p<0.05). 또한 건물 분해율은 3 및 24시간에 T4구에서 T1구와 비교하여 높았다(p<0.05). 결과적으로 농산부산물인 청치와 비지의 사용은 옥수수와 대두박을 일부 대체하기 위해 사용가능할 것으로 판단된다.

Keywords

References

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