• Title/Summary/Keyword: Fomitella fraxinea

Search Result 32, Processing Time 0.029 seconds

Screening of mushroom having anti-angiogenic activity for development of new cancer metastasis Inhibitor (버섯으로부터 새로운 암전이 억제물질 개발을 위한 혈관신생 억제물질의 탐색)

  • Jeong, Seung-Chan;Kim, Jae-Ho;Park, Jeong-Sik;Lee, Jong-Soo
    • Journal of Mushroom
    • /
    • v.1 no.1
    • /
    • pp.44-47
    • /
    • 2003
  • This study was carried out to obtain new cancer metastasis inhibitor from mushrooms. Extracts from 52 isolates belong to 7 species of mushrooms were prepared by water, ethanol and methanol extractions and its anti-angiogenic activity were investigated by choriollantoic membrane(CAM) assay. Water extracts of Fomitella fraxinea ASI 17003 and ASI 17009 fruiting bodies, ethanol extract of Pholiota sp. ASI 24008 and Grifola frondosa ASI 9017 fruiting bodies and methanol extract of Inonotus obliquus ASI 74012 mycelia had the potential anti-angiogenic activity of 62.5%~68.8%. Finally, Pholiota sp. ASI 24008 was selected as a producer of cancer metastasis inhibitor on the basis of their solid yield and anti-angiogenic activity, etc.

  • PDF

Biological Activities of Culture Broth of Some Wood Rotting Basidiomycetes -Antimicrobial, plant growth regulatory, antitumor, and enzymatic activities- (목재부후성 담자균류 배양균사체의 생물활성 연구 -항균활성, 식물생장조절활성, 항암활성, 효소활성탐색-)

  • Cho, Soo-Muk;Yu, Seung-Hun;Shin, Gwan-Chull
    • The Korean Journal of Mycology
    • /
    • v.24 no.1 s.76
    • /
    • pp.17-24
    • /
    • 1996
  • For screening of biological activities of culture broth of some wood rotting basidiomycetes, antimicrobial activity, plant growth regulating activity, antitumor activity, and various enzyme activities were checked. Coriolus versicolor 5129 and C. pubescens 5131 strains showed inhibition activity against gram-positive bacteria and Lenzites betulina 8029 strain showed the activity against gram-negative bacteria. L. betulina 8085 inhibited the growth of both bacteria and plant pathogenic fungi. All of tested basidiomycetes inhibited the germination and growth of radish and cabbage at concentration of 0.8ml/ml. Especially, Fomitopsis pinicolor 8059 and Fomitella fraxinea 8084 showed strong inhibition activity. In contrast, Bjerkandera adusta 8054 stimulated the growth of cabbage and radish at concentration of 0.4 and 0.2ml/ml. All polysaccharides from tested basidiomycetes showed anti-tumor activity against sarcoma 180 and the stronger antitumor activity was observed in L. betulina 8029 and unidentified 8058 strain. All tested basidiomycetes had also an ability to degrade cellulose and lignin.

  • PDF

Cultural Characteristics of Fomitella fraxinea (Fr.) Imaz. (장수버섯의 배양적(培養的) 특성(特性))

  • Chang, Hyun-You;Cha, Dong-Yeul;Kang, An-Seok;Hong, In-Pyo;Kim, Kwang-Po;Seok, Soon-Ja;Ryu, Young-Jin;Sung, Jae-Mo
    • The Korean Journal of Mycology
    • /
    • v.23 no.3 s.74
    • /
    • pp.238-245
    • /
    • 1995
  • A good mycelial growth of F. fraxinea was observed on CDA medium. The optimum temperature and pH for the mycelial growth of F. fraxinea was at $30^{\circ}C$ and pH 6.0, respectively. Glucose was found to be the best carbon source and arginine was favored as nitrogen source. When the basal medium was supplemented with organic acids, the best growth was shown in succinic acid and the poor growth was shown in oxalic acid. Thiamine.HCl showed the best results on the growth of this fungus on basal medium. Mycelial growth of F. fraxinea was quite good when oak tree sawdust was used to cultural substrates. The best mycelial growth was observed when 20% of rice bran was added as a supplement on sawdust substrates. Higher yield of F. fraxinea was observed on the medium with oak tree and acacia sawdust.

  • PDF

Biological Detoxification of Lacquer Tree (Rhus verniciflua Stokes) Stem Bark by Mushroom Species

  • Choi, Han-Seok;Kim, Myung-Kon;Park, Hyo-Suk;Yun, Sei-Eok;Mun, Sung-Phil;Kim, Jae-Sung;Sapkota, Kumar;Kim, Seung;Kim, Tae-Young;Kim, Sung-Jun
    • Food Science and Biotechnology
    • /
    • v.16 no.6
    • /
    • pp.935-942
    • /
    • 2007
  • The stem bark of Rhus verniciflua (RVSB) has been used in herbal medicine to treat diabetes mellitus and stomach ailments for thousands of years in Korea, despite its content of the plant allergen, urushiol. A new biological approach for the removal of urushiol from RVSB using mushrooms is described. All mushroom species (11 sp.) employed in this study were able to grow on RVSB, although the growth rate (mm/day) was lower than the control (sawdust). The components of urushiol congeners [C15 triene (m/z 314), C15 diene (m/z 316), C15 monoene (m/z 318), and C15 saturated (m/z 320)] were purified by HPLC and identified by GC-MS. A C15:3 (3-pentadecatrienly catechol) was found to be most abundant in RVSB. Urushiol analogues decreased remarkably from 154.15 to 10.73 mg/100 g (approximately 93%) by Fomitella fraxinea, whereas Trametes vercicolor showed only a 1.46% degradation capacity despite its 2 fold higher growth rate. Similarly, laccase activity was found to be high for F. fraxinea and low for T. vercicolor. Moreover, approximately 98% detoxification was accomplished by F. fraxinea cultivated on RVSB supplemented with 20%(w/w) rice bran. These findings suggest that mushrooms can be used in the detoxification of RVSB.

Antioxidant Activity of Ergosterol Peroxide (5,8-Epidioxy-$5\alpha,8\alpha$-ergosta-6,22E-dien-3$\beta$-ol) in Armillariella mellea

  • 김상욱;박상신;민태진;유국현
    • Bulletin of the Korean Chemical Society
    • /
    • v.20 no.7
    • /
    • pp.819-823
    • /
    • 1999
  • Antioxidant activities of various mushroom fruiting bodies were investigated in vitro. Among the mushroom extracts examined, Armillariella mellea, Daedalea dickinsi, Fomitella fraxinea and Pleurotus cornusopiae markedly exhibited inhibition on lipid peroxidaton of rat liver microsomes. Ergosterol peroxide (5,8-epidioxy-5α,8α-ergosta-6,22E-dien-3β-ol), antioxidant from A. mellea, was isolated by solvent extraction, silica gel column chromatography and recrystallization. The structure of the compound was determined by NMR, GC/MS and X-ray crystallography. Ergosterol peroxide showed potent inhibition on lipid peroxidation and exhibited higher antioxidant activity than well-known antioxidants, α-tocopherol and thiourea.

Physicochemical Properties and Physiological Activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea (장수버섯 균사체가 배양된 옻피의 이화학적 특성 및 생리활성)

  • Choi, Han-Seok;Kim, Bo-Hyun;Yeo, Soo-Hwan;Jeong, Seok-Tae;Choi, Ji-Ho;Park, Hyo-Suk;Kim, Myung-Kon
    • The Korean Journal of Mycology
    • /
    • v.38 no.2
    • /
    • pp.172-178
    • /
    • 2010
  • The contents of proximate composition, free amino acids and phenolic acids in the Fomitella fraxinea cultivated-Rhus verniciflua stem bark(FRVSB), and its adipogenesis effect were investigated. The proximate composition(%) of FRVSB was as follows: moisture(7.64), ash(6.30), crude fat(3.86), crude protein(3.59) and sugar(not detected); while Rhus verniciflua stem bark(RVSB) contained 1.64, 8.09, 7.28, 6.48 and 5.39, respectively. The total free amino acids concentration was 97.41 mg% in FRVSB and 71.91 mg% in RVSB. Phosphoserine(55.06 mg%), ammonia(17.84mg%) and aspartic acid(6.05mg%) were predominant amino acids. The content of total phenolic acids was 422.89 ppm in ethanol extract and 283.86 ppm in water extract, with syringic and gallic acid as the main component. The FRVSB extracts showed a potent free radical scavenging activity for DPPH(2,2-diphenyl-1-picrylhydrazyl hydrate) with $IC_{50}$ of $28.54\;{\mu}g$(EtOH) and $54.70\;{\mu}g$(water), respectively, whereas $IC_{50}$ value of gallic acid was $1.84\;{\mu}g$. The protective effect of both ethanol and water extract the extracts against UV-induced oxidative stress in NIH3T3 was observed. The water extracts of FRVSB may promote adipogenesis in 3T3-L1 cells.

Effect of the Extracts from Fermented-Rhus verniciflua Stem Bark with Fomitella fraxinea on the Growth and Enzyme Activity of Soybean Product-fermenting Microorganisms (장수버섯 배양으로 제조한 발효옻 추출물이 장류 미생물의 증식 및 효소활성에 미치는 영향)

  • Choi, Han-Seok;Yeo, Soo-Hwan;Jeong, Seok-Tae;Choi, Ji-Ho;Kang, Ji-Eun;Kim, Myung-Kon
    • The Korean Journal of Mycology
    • /
    • v.40 no.4
    • /
    • pp.235-243
    • /
    • 2012
  • We studied the effect of fermented Rhus verniciflua stem bark (FRVSB) extract (used in herbal med-icine by Koreans) on the microbial growth and enzyme activity of 12 soybean-fermenting microorganisms, including Bacillus spp., lactic acid bacteria, yeast, and other harmful bacteria. The ethanol and methanol extracts of FRVSB inhibited the growth of Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, and Zygosaccharomyces rouxii, and in the disk diffusion assay, their inhibition zone diameters were 11.06-12.23, 12.32-18.38, 11.47-11.84, and 13.59-14.21 mm, respectively. The water extract did not show any inhibitory effect. In fact, the water extract addition enhanced the growth of B. subtilis and B. licheniformis by 1.3-4.5 fold and that of B. cereus by 1.2-1.4 fold. However, the water extract did not affect the growth of Lactobacillus plantarum, Lactobacillus mesenteroides, Saccharomyces cer-evisiae, and Escherichia coli. The addition of water extract increased the amylase and protease activity of B. subtilis and B. licheniformis.

Enzyme activity of Basidiomycetes products in each cereals (곡물별 담자균 배양물의 효소활성)

  • Park, Hyo-Suk;Kim, Bo-Hyun;Choi, Han-Seok;Kim, Joong-Man;Kim, Myung-Kon
    • Journal of Mushroom
    • /
    • v.8 no.3
    • /
    • pp.102-108
    • /
    • 2010
  • In this study, growth rates and enzyme activities of mushroom mycelium were investigated in each cereals. Cultivation on hulled barley resulted in slightly faster mycelial growth as compared to other substrates. Enzyme activities were measured in different periods. In result, ${\alpha}$-amylase activity was higher in Pleurotus ostreatus and Phellinus linteus cultured cereals, whereas ${\beta}$-amylase activity was higher in Pleurotus ostreatus and Fomitella fraxinea cultured. Protease level did not affect kinds of cereal except Phellinus linteus. The color values lightness decrease by Basidiomycetes cultivation and redness and yellowness increased.

  • PDF

Detection of Platelet Aggregation Inhibitors and Fibrinolytic Substances from Mushrooms (버섯류로부터 혈소판 응집억제물질과 혈전용해물질의 탐색)

  • Park, Jeong-Sik;Hyun, Kwang-Wook;Seo, Seung-Bo;Cho, Soo-Muk;Yoo, Chang-Hyun;Lee, Jong-Soo
    • The Korean Journal of Mycology
    • /
    • v.31 no.2
    • /
    • pp.114-116
    • /
    • 2003
  • Platelet aggregation inhibitory and fibrinolytic activities of water and ethanol extracts from mushrooms were studied. The highest platelet aggregation inhibitory activity was 81.2% in the ethanol extract from fruiting body of Inonotus obliquus ASI 74006 and also were high in the ethanol extract from fruiting bodies of Fomitella fraxinea. The ethanol extract from the mycelia of Agaricus blazei Murill. ASI 1174 showed the strongest fibrinolytic activity as 9.6 unit. However fibrinolytic activities of other mushrooms were low or negligible.

Effect of Urushiol-Free Extracts from Fermented-Rhus verniciflua Stem Bark with Fomitella fraxinea on the Fermentation Characteristics of Doenjang (Soybean Paste) (장수버섯 배양법에 의해 urushiol이 제거된 발효옻 추출물이 된장발효에 미치는 영향)

  • Choi, Han-Seok;Jeong, Seok-Tae;Choi, Ji-Ho;Kang, Ji-Eun;Kim, Eugene;Noh, Jong-Min;Kim, Myung-Kon
    • The Korean Journal of Mycology
    • /
    • v.40 no.4
    • /
    • pp.244-253
    • /
    • 2012
  • The effect of fermented Rhus verniciflua stem bark (FRVSB) extract on the microbial count, enzyme activity, concentrations of free amino acids and organic acids, and physiochemical properties of doenjang (soybean paste) was evaluated during brine fermentation. The FRVSB extract increased the total free amino acid concentration by 1.3-3.1-fold on the $42^{nd}$ day of brine fermentation. After the filtration of brine, the following microbial counts were obtained in the doenjang: bacteria, $0.3{\times}10^8-12.0{\times}10^8$ cfu/g; mold, $3.0{\times}10^4-21.0{\times}10^4$ cfu/g; yeast, $1.0{\times}10^4-2.0{\times}10^4$ cfu/g; Escherichia coli, not detected; and Bacillus cereus, $3.0{\times}10^2-25.0{\times}10^2$ cfu/g. The FRVSB extract addition enhanced the protein and starch degrading activity by 13.8-26.0% and 16.1-35.1%, respectively. The extract increased the total free amino acid content by 1.4-3.0-fold. Lactic acid, acetic acid, and pyroglutamic acid were the predominant organic acids in doenjang. Moreover, the proximate composition, pH, moisture, ash, salt, and amino nitrogen content were increased.