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Biological Detoxification of Lacquer Tree (Rhus verniciflua Stokes) Stem Bark by Mushroom Species  

Choi, Han-Seok (Department of Food Science and Technology, Chonbuk National University)
Kim, Myung-Kon (Department of Industrial Crop Production and Processing, Iksan National College)
Park, Hyo-Suk (Wonkwang University, Graduate School of Agricultural Chemistry)
Yun, Sei-Eok (Department of Food Science and Technology, Chonbuk National University)
Mun, Sung-Phil (Wood Science and Technology, Chonbuk National University)
Kim, Jae-Sung (Department of Biotechnology, Chosun University)
Sapkota, Kumar (Department of Biotechnology, Chosun University)
Kim, Seung (Department of Biotechnology, Chosun University)
Kim, Tae-Young (Rural Resource Development Institute, National Institute of Agricultural Science and Technology, RDA)
Kim, Sung-Jun (Department of Biotechnology, Chosun University)
Publication Information
Food Science and Biotechnology / v.16, no.6, 2007 , pp. 935-942 More about this Journal
Abstract
The stem bark of Rhus verniciflua (RVSB) has been used in herbal medicine to treat diabetes mellitus and stomach ailments for thousands of years in Korea, despite its content of the plant allergen, urushiol. A new biological approach for the removal of urushiol from RVSB using mushrooms is described. All mushroom species (11 sp.) employed in this study were able to grow on RVSB, although the growth rate (mm/day) was lower than the control (sawdust). The components of urushiol congeners [C15 triene (m/z 314), C15 diene (m/z 316), C15 monoene (m/z 318), and C15 saturated (m/z 320)] were purified by HPLC and identified by GC-MS. A C15:3 (3-pentadecatrienly catechol) was found to be most abundant in RVSB. Urushiol analogues decreased remarkably from 154.15 to 10.73 mg/100 g (approximately 93%) by Fomitella fraxinea, whereas Trametes vercicolor showed only a 1.46% degradation capacity despite its 2 fold higher growth rate. Similarly, laccase activity was found to be high for F. fraxinea and low for T. vercicolor. Moreover, approximately 98% detoxification was accomplished by F. fraxinea cultivated on RVSB supplemented with 20%(w/w) rice bran. These findings suggest that mushrooms can be used in the detoxification of RVSB.
Keywords
detoxification; laccase; lacquer tree; mushroom; urushiol;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
Times Cited By SCOPUS : 7
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