• 제목/요약/키워드: Fluorescence measurement technique

검색결과 71건 처리시간 0.024초

평면 이미지 기법을 이용한 분무 특성 해석 (The Spray Characterization Using Planar Imaging Technique)

  • 이경진;정기훈;윤영빈;정경석;정인석
    • 대한기계학회논문집B
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    • 제24권1호
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    • pp.93-101
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    • 2000
  • The characteristics of spray nozzle have been quantified with the measurement of fluorescence and Mie scattering images. To correct the attenuation of the incident light sheet, a sequential double-pass light sheet system and the geometrical averaging of two images was implemented. Quantitative mass flux distribution of spray was obtained from fluorescence image. 3-D image is reconstructed using 2-D radial images. Sauter mean diameter (SMD) distribution was determined using the ratio of fluorescence signal intensity and Mie scattering signal intensity and the values were quantified with PDP A data. The measurement of mass flux and SMD using planar imaging technique agee with PDP A data fairly well in the low density region. However, in dense region, there are significant errors caused by secondary scattering. It was found that the planar imaging technique provides many advantages over the point measurement technique, such as PDP A, and can be implemented for quantitative measurement, especially in low density region.

Optical Filter Design for Fluorescence Technique Based Phycocyanin Measurement Sensor Used In Water Treatment Plants

  • Mariappan, Vinayagam;Lee, Sung Hwa;Yang, Seungyoun;Kim, Jintae;Lee, Minwoo
    • International Journal of Internet, Broadcasting and Communication
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    • 제10권2호
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    • pp.45-50
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    • 2018
  • Recently the water management department advised the water treatment industry to focus on deploy the chemical free and the environmentally responsible process to adopt on water treatment plants in every country. In this objective, water treatment process started using ultrasonic based phycocyanin extraction with fluorescence measurement techniques to detect the change in the yield of phycocyanin. This paper propose the design of optical filter model for fluorescence technique based immersive optical phycocyanin measurement sensor design. The proposed design uses the multi-wavelength sensor module for irradiating part, and this plays a role of removing a wavelength band other than 590 ~ 620 nm. The preliminary study on immersed phycocyanin sensor, the fluorescence value of picocyanin according to the ultrasonic intensity, treatment time and number of cells was measured using JM phycocyanin module to emulate the proposed design, and were compared performance of the proposed sensor emulation. In this design, the phycocyanin fluorescence value increased about 2.1 ~ 4.7 times as the ultrasonic treatment time increased as compared with JM phycocyanin module, and the phycocyanin fluorescence value within the analysis range was obtained by ultrasonic treatment within one minute.

온도 민감 형광을 이용한 마이크로 스케일 표면온도 측정 (Surface Temperature Measurement in Microscale with Temperature Sensitive Fluorescence)

  • 정운섭;김성욱;김호영;유정열
    • 대한기계학회논문집B
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    • 제30권2호
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    • pp.153-160
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    • 2006
  • A technique for measuring surface temperature field in micro scale is newly proposed, which uses temperature-sensitive fluorescent (TSF) dye coated on the surface and is easily implemented with a fluorescence microscope and a CCD camera. The TSF dye is chosen among mixtures of various chemical compositions including rhodamine B as the fluorescent dye to be most sensitive to temperature change. In order to examine the effectiveness of this temperature measurement technique, numerical analysis and experiment on transient conduction heat transfer for two different substrate materials, i. e., silicon and glass, are performed. In the experiment, to accurately measure the temperature with high resolution temperature calibration curves were obtained with very fine spatial units. The experimental results agree qualitatively well with the numerical data in the silicon and glass substrate cases so that the present temperature measurement method proves to be quite reliable. In addition, it is noteworthy that the glass substrate is more appropriate to be used as thermally-insulating locally-heating heater in micro thermal devices. This fact is identified in the temperature measuring experiment on the locally-heating heaters made on the wafer of silicon and glass substrates. Accordingly, this technique is capable of accurate and non-intrusive high-resolution measurement of temperature field in microscale.

Calibration-free real-time organic film thickness monitoring technique by reflected X-Ray fluorescence and compton scattering measurement

  • Park, Junghwan;Choi, Yong Suk;Kim, Junhyuck;Lee, Jeongmook;Kim, Tae Jun;Youn, Young-Sang;Lim, Sang Ho;Kim, Jong-Yun
    • Nuclear Engineering and Technology
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    • 제53권4호
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    • pp.1297-1303
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    • 2021
  • Most thickness measurement techniques using X-ray radiation are unsuitable in field processes involving fast-moving organic films. Herein, we propose a Compton scattering X-ray radiation method, which probes the light elements in organic materials, and a new simple, non-destructive, and non-contact calibration-free real-time film thickness measurement technique by setting up a bench-top X-ray thickness measurement system simulating a field process dealing with thin flexible organic films. The use of X-ray fluorescence and Compton scattering X-ray radiation reflectance signals from films in close contact with a roller produced accurate thickness measurements. In a high-thickness range, the contribution of X-ray fluorescence is negligible, whereas that of Compton scattering is negligible in a low-thickness range. X-ray fluorescence and Compton scattering show good correlations with the organic film thickness (R2 = 0.997 and 0.999 for X-ray fluorescence and Compton scattering, respectively, in the thickness range 0-0.5 mm). Although the sensitivity of X-ray fluorescence is approximately 4.6 times higher than that of Compton scattering, Compton scattering signals are useful for thick films (e.g., thicker than ca. 1-5 mm under our present experiment conditions). Thus, successful calibration-free thickness monitoring is possible for fast-moving films, as demonstrated in our experiments.

Design of Optical Biological Sensor for Phycocyanin Parameters Measurement using Fluorescence Technique

  • Lee, Sung Hwa;Mariappan, Vinayagam;Won, Dong Chan;Ann, Myungsuk;Yang, Seungyoun
    • International journal of advanced smart convergence
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    • 제5권2호
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    • pp.73-79
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    • 2016
  • Remote sensing and measurement are of paramount importance of providing information on the state of water quality in water bodies. The formation and growth of cyanobacteria is of serious concern to in land aquatic life forms and human life. The main cause of water quality deterioration stems from anthropogenic induced eutrophication. The goal of this research to quantify and determine the spatial distribution of cyanobacteria concentration in the water using remote sensing technique. The standard approach to measure water quality based on the direct measurement of the fluorescence of the chlorophyll a in the living algal cells and the same approach used to detect the phycobilin pigments found in blue-green algae (a.k.a. cyanobacteria), phycocyanin and phycoerythrin. This paper propose the emerging sensor design to measure the water quality based on the optical analysis by fluorescence of the phycocyanin pigment. In this research, we developed an method to sense and quantify to derive phycocyanin intensity index for estimating cyanobacteria concentrations. The development of the index was based on the reflectance difference between visible light band 620nm and 665nm. As a result of research this paper presents, an optical biological sensor design information to measure the Phycocyanin parameters in water content.

Measurements of Temperature and Flow Fields with Sub-Millimeter Spatial Resolution Using Two-Color Laser Induced Fluorescence (LIF) and Micro-Particle Image Velocimetry (PIV)

  • Kim Hyun Jung
    • Journal of Mechanical Science and Technology
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    • 제19권2호
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    • pp.716-727
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    • 2005
  • Comprehensive measurements for velocity and temperature fields have been conducted. A Micro PIV 2-color LIF system have been setup to measure the buoyancy driven fields in a 1-mm heated channel with low Grashof-Prandtl numbers [$86]. Fluorescence microscopy is combined with an MPIV system to obtain enough intensity images and clear pictures from nano-scale fluorescence particles. The spatial resolution of the Micro PIV system is $75{\mu}m\;by\;67{\mu}m$ and error due to Brownian motion is estimated $1.05\%$. Temperature measurements have achieved the $4.7\;{\mu}m$ spatial resolution with relatively large data uncertainties the present experiment. The measurement uncertainties have been decreased down to less than ${\pm}1.0^{\circ}C$ when measurement resolution is equivalent to $76\;{\mu}m$. Measured velocity and temperature fields will be compared with numerical results to examine the feasibility of development as a diagnostic technique.

High-speed Three-dimensional Surface Profile Measurement with the HiLo Optical Imaging Technique

  • Kang, Sewon;Ryu, Inkeon;Kim, Daekeun;Kauh, Sang Ken
    • Current Optics and Photonics
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    • 제2권6호
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    • pp.568-575
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    • 2018
  • Various techniques to measure the three-dimensional (3D) surface profile of a 3D micro- or nanostructure have been proposed. However, it is difficult to apply such techniques directly to industrial uses because most of them are relatively slow, unreliable, and expensive. The HiLo optical imaging technique, which was recently introduced in the field of fluorescence imaging, is a promising wide-field imaging technique capable of high-speed imaging with a simple optical configuration. It has not been used in measuring a 3D surface profile although confocal microscopy originally developed for fluorescence imaging has been adapted to the field of 3D optical measurement for a long time. In this paper, to the best of our knowledge, the HiLo optical imaging technique for measuring a 3D surface profile is proposed for the first time. Its optical configuration and algorithm for a precisely detecting surface position are designed, optimized, and implemented. Optical performance for several 3D microscale structures is evaluated, and it is confirmed that the capability of measuring a 3D surface profile with HiLo optical imaging technique is comparable to that with confocal microscopy.

형광 나노 포러스 박막을 이용한 표면 온도 센서의 제작 및 성능 연구 II (Fabrication and Performance Investigation of Surface Temperature Sensor Using Fluorescent Nanoporous Thin Film II)

  • 김현정;유재석;박진일
    • 설비공학논문집
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    • 제25권12호
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    • pp.674-678
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    • 2013
  • We present a non-invasive technique to the measure temperature distribution in nano-sized porous thin films by means of the two-color laser-induced fluorescence (2-LIF) of rhodamine B. The fluorescence induced by the green line of a mercury lamp with the makeup of optical filters was measured on two separate color bands. They can be selected for their strong difference in the temperature sensitivity of the fluorescence quantum yield. This technique allows for absolute temperature measurements by determining the relative intensities on two adequate spectral bands of the same dye. To measure temperature fields, Silica (SiO2) nanoporous structure with 1-um thickness was constructed on a cover glass, and fluorescent dye was absorbed into these porous thin films. The calibration curves of the fluorescence intensity versus temperature were measured in a temperature range of $10-60^{\circ}C$, and visualization and measurement of the temperature field were performed by taking the intensity distributions from the specimen for the temperature field.

Stereo-PIV/LIF의 속도장과 농도장 동시측정 기법을 이용한 러쉬톤 교반기내 난류특성에 관한 연구 (A Study on the Turbulent Characteristics of Rushton Turbine Mixer by Simultaneous Measurement of Velocity and Concentration field with Stereo-PIV/PLIF Technique)

  • 민영욱;김윤기;김경천
    • 대한기계학회논문집B
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    • 제28권3호
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    • pp.365-370
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    • 2004
  • Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereo Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K${\times}$2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent flow around Rushton turbine were identified by the calculation of synchronized data of the velocity field and concentration field.