• 제목/요약/키워드: Fluorescence measurement

검색결과 322건 처리시간 0.027초

자외선 형광 방출비 평가에 의한 전기 절연유의 산화측정 (Measurement of Electrical Insulating Oil Oxidation by Evaluating the UV Fluorescence Emission Ratio)

  • ;공호성;한흥구
    • Tribology and Lubricants
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    • 제29권1호
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    • pp.51-55
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    • 2013
  • In this work, a new fluorescence emission measurement technology was introduced and experimentally compared with other measurement methods, such as the titration method and IR spectroscopy, to validate it for the oil oxidation measurement of electrical insulating oil. The oxidation characteristics of insulating oil were found to be fairly represented by the titration method and IR spectroscopy, and the results are comparable to a change in the fluorescence emission ratio that is defined as the shift in fluorescence intensity in the measured wavelength range. The result also shows that by the measurement of fluorescence emission ratio, it is possible to detect the oxidation of oil relatively earlier than by other methods. This study suggests that the developed technology can provide sufficient information for evaluating the insulating oil quality, and that the developed FER sensor can be used as an effective condition monitoring device of electrical insulating oil oxidation.

Optical Filter Design for Fluorescence Technique Based Phycocyanin Measurement Sensor Used In Water Treatment Plants

  • Mariappan, Vinayagam;Lee, Sung Hwa;Yang, Seungyoun;Kim, Jintae;Lee, Minwoo
    • International Journal of Internet, Broadcasting and Communication
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    • 제10권2호
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    • pp.45-50
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    • 2018
  • Recently the water management department advised the water treatment industry to focus on deploy the chemical free and the environmentally responsible process to adopt on water treatment plants in every country. In this objective, water treatment process started using ultrasonic based phycocyanin extraction with fluorescence measurement techniques to detect the change in the yield of phycocyanin. This paper propose the design of optical filter model for fluorescence technique based immersive optical phycocyanin measurement sensor design. The proposed design uses the multi-wavelength sensor module for irradiating part, and this plays a role of removing a wavelength band other than 590 ~ 620 nm. The preliminary study on immersed phycocyanin sensor, the fluorescence value of picocyanin according to the ultrasonic intensity, treatment time and number of cells was measured using JM phycocyanin module to emulate the proposed design, and were compared performance of the proposed sensor emulation. In this design, the phycocyanin fluorescence value increased about 2.1 ~ 4.7 times as the ultrasonic treatment time increased as compared with JM phycocyanin module, and the phycocyanin fluorescence value within the analysis range was obtained by ultrasonic treatment within one minute.

Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures

  • Chattopadhyay, Saurabh;Bisaria, V.S.;Scheper, T.;Srivastava, A.K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제7권6호
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    • pp.331-334
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    • 2002
  • Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation In a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

아세톤 형광을 이용한 공연비 측정 기법 연구 (An Experimental Investigation of Air Fuel Ratio Measurement using Laser Induced Acetone Fluorescence)

  • 박승재;허환일;오승묵
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2002년도 학술대회지
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    • pp.353-356
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    • 2002
  • Planar laser induced fluorescence(PLIF) has been widely used to obtain two dimensional fuel distribution. Preliminary investigation was performed to measure quantitative air excess ratio distribution in an engine fueled with LPG. It is known that fluorescence signal from acetone as a fluorescent tracer is less sensitive to oxygen quenching than other dopants. Acetone was excited by KrF excimer laser (248nm) and its fluorescence image was acquired by ICCD camera with a cut-of filter to suppress Mie scattering from the laser light. For the purpose of quantifying PLIF signal, an image processing method including the correction of laser sheet beam profile was suggested. Raw images were divided by each intensity of laser energy and profile of laser sheet beam. Inhomogeneous fluorescence images scaled with the reference data, which was taken by a calibration process, were converted to air excess ratio distribution. This investigation showed instantaneous quantitative measurement of planar air excess ratio distribution for gaseous fuel.

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Measurements of Temperature and Flow Fields with Sub-Millimeter Spatial Resolution Using Two-Color Laser Induced Fluorescence (LIF) and Micro-Particle Image Velocimetry (PIV)

  • Kim Hyun Jung
    • Journal of Mechanical Science and Technology
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    • 제19권2호
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    • pp.716-727
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    • 2005
  • Comprehensive measurements for velocity and temperature fields have been conducted. A Micro PIV 2-color LIF system have been setup to measure the buoyancy driven fields in a 1-mm heated channel with low Grashof-Prandtl numbers [$86]. Fluorescence microscopy is combined with an MPIV system to obtain enough intensity images and clear pictures from nano-scale fluorescence particles. The spatial resolution of the Micro PIV system is $75{\mu}m\;by\;67{\mu}m$ and error due to Brownian motion is estimated $1.05\%$. Temperature measurements have achieved the $4.7\;{\mu}m$ spatial resolution with relatively large data uncertainties the present experiment. The measurement uncertainties have been decreased down to less than ${\pm}1.0^{\circ}C$ when measurement resolution is equivalent to $76\;{\mu}m$. Measured velocity and temperature fields will be compared with numerical results to examine the feasibility of development as a diagnostic technique.

Calibration-free real-time organic film thickness monitoring technique by reflected X-Ray fluorescence and compton scattering measurement

  • Park, Junghwan;Choi, Yong Suk;Kim, Junhyuck;Lee, Jeongmook;Kim, Tae Jun;Youn, Young-Sang;Lim, Sang Ho;Kim, Jong-Yun
    • Nuclear Engineering and Technology
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    • 제53권4호
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    • pp.1297-1303
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    • 2021
  • Most thickness measurement techniques using X-ray radiation are unsuitable in field processes involving fast-moving organic films. Herein, we propose a Compton scattering X-ray radiation method, which probes the light elements in organic materials, and a new simple, non-destructive, and non-contact calibration-free real-time film thickness measurement technique by setting up a bench-top X-ray thickness measurement system simulating a field process dealing with thin flexible organic films. The use of X-ray fluorescence and Compton scattering X-ray radiation reflectance signals from films in close contact with a roller produced accurate thickness measurements. In a high-thickness range, the contribution of X-ray fluorescence is negligible, whereas that of Compton scattering is negligible in a low-thickness range. X-ray fluorescence and Compton scattering show good correlations with the organic film thickness (R2 = 0.997 and 0.999 for X-ray fluorescence and Compton scattering, respectively, in the thickness range 0-0.5 mm). Although the sensitivity of X-ray fluorescence is approximately 4.6 times higher than that of Compton scattering, Compton scattering signals are useful for thick films (e.g., thicker than ca. 1-5 mm under our present experiment conditions). Thus, successful calibration-free thickness monitoring is possible for fast-moving films, as demonstrated in our experiments.

Beam Induced Fluorescence 모니터를 이용한 KIRAMS-13 싸이클로트론의 양성자빔 횡단면 측정 (Transverse Profile Measurement of Proton Beam using the Beam Induced Fluorescence Monitor in KIRAMS-13 Cyclotron)

  • 남순권;김기범
    • 한국진공학회지
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    • 제18권6호
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    • pp.418-425
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    • 2009
  • 권역별 싸이클로트론연구소에 설치되어 있는 KIRAMS-13 싸이클로트론에서 안정적이고 최적화된 양성자빔을 인출하기 위해서는 양성자빔의 횡단면 측정이 필수적이다. 빔의 횡단면 측정장치 중 비파괴적인 방법인 BIF(Beam Induced Fluorescence) 모니터는 그 구조가 상대적으로 간단하고 빔의 에너지 손실을 최소화하며 실시간 측정이 가능하다는 장점이 있다. 이 연구에서는 KIRAMS-13 싸이클로트론에서 양성자빔의 횡단면 측정을 위한 BIF 모니터를 설계하고 제작한 후 13 MeV 양성자빔 조사실험을 통하여 양성자 빔의 중심 및 반경을 측정하였다.