[KSCI] Korea Science Citation Index Service

Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures

Chattopadhyay, Saurabh (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology)
Bisaria, V.S. (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology)
Scheper, T. (Institut fuer Technische Chemie)
Srivastava, A.K. (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology)

Publication Information

Biotechnology and Bioprocess Engineering:BBE / v.7, no.6, 2002 , pp. 331-334 More about this Journal

Abstract

Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation In a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

Keywords

Podophyllum hexandrum; MADH measurement; conductivity measurement; on-line bio-mass measurement;

Citations & Related Records

Times Cited By SCOPUS : 1

Reference
Cited By SCOPUS

1	The production of cytotoxic lignans by plant cultures. / [ Petersen M.;A. W. Alfermann ] / Appl. Microbiol. Biotechnol. DOI ScienceOn
2	Online monitoring of cell growth in plant tissue cultures by conductometry. / [ Taya M.;M. Hegglin;J. E. Prenosil;J. R. Bourne ] / Enzyme Microb. Technol. DOI ScienceOn
3	NADH fluorescence in a carbon limited fermentation / [ Srivastava A. K.;B. Volesky ] / Biotechnol. Bioeng. DOI
4	Optimization of culture parameters for production of podophyllotoxin in suspension culture of Podophyllum hexandrum. / [ Chattopadhyay S.;A. K. Srivastava;V. S. Bisaria ] / Appl. Biochem. Biotechnol.
5	A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures / [ Blom T. J. M.;W. Kreis;F. V. Iren;K. R. Libbenga ] / Plant Cell. Rep.
6	Culture fluorescence dynamics in Xanthomonas campestris / [ Sriram G.;G. K. Sureshkumar ] / Process Biochem. DOI
7	Use of NAD(P)H-fluorescence for monitoring the response of starved cells of Catharanthus roseus in suspension to metabolic perturbations. / [ Asali E. C.;R. Muthurasan;A. E. Humphrey ] / J. Biotechnol. DOI ScienceOn
8	Development of suspension culture of Podophyllum hexandrum for production of podophyllotoxin. / [ Chattopadhyay S.;A. K. Srivastava;S. S. Bhojwani;V. S. Bisaria ] / Biotechnol. Lett. DOI ScienceOn
9	Plant cell reactors: A perspective / [ Panda A. K.;S. Mishra;V. S. Bisaria;S. S. Bhojwani ] / Enzyme Microb. Technol. DOI ScienceOn
10	Biomass estimation in plant cell cultures: A neural net-work approach / [ Albiol J.;C. Campmajo;C. Casas;M. Poch ] / Biotechnol. Prog.
11	Transgenic hairy roots: Recent trends and applications / [ Giri A. and M. L. Narasu ] / Biotechnol. Adv. DOI ScienceOn
12	Fluorometric technique for monitoring changes in the level of reduced nicotinamide nucleotides in continuous cultures of microorganisms / [ Harrison D. E. F.;B. Chance ] / Appl. Microb.
13	Determination of growth rate for plant cell cultures: Comparative studies. / [ Ryu D. D. Y.;S. O. Lee;R. J. Romani ] / Biotechnol. Bioeng. DOI
14	On-line fluorescence measurements in assessing culture metabolic activities / [ Srivastava A. K. and B. Volesky ] / Appl. Microbiol. Biotechnol.
15	A revised medium for rapid growth and bioassay with tobacco tissue cultures. / [ Murashige T.;F. Skoog ] / Physiol. Plant. DOI
16	Studies on osmolarity, conductivity and mass transfer for selection of a bioreactor for Tagetes patula L. hairy roots / [ Suresh B.;T. Rajasekaran;S. R. Rao;K. S. M. S. Raghavarao;G. A. Ravishankar ] / Process Biochem. DOI
17	Fluorescence spectrometry of reduced phosphopyridine nucleotide in intact cells in the near ultraviolet and visible region / [ Duysens L. N. M.;J. Amesz ] / Biochem. Biophys. Acta DOI ScienceOn
18	On-line monitoring of cell concentration of Perilla frutescens in a bioreator / [ Zhong J. J.;K. Fujiyama;T. Seki;T. Yoshida ] / Biotechnol. Bioeng. DOI ScienceOn
19	Monitoring of NADH-dependent culture fluorescence during the cultivation of Escherichia coli / [ Scheper T.;A. Gebauer;K. Schugerl ] / Chem. Eng. J. DOI