• Title/Summary/Keyword: Fluorescence measurement

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Measurement of Electrical Insulating Oil Oxidation by Evaluating the UV Fluorescence Emission Ratio (자외선 형광 방출비 평가에 의한 전기 절연유의 산화측정)

  • Wicaksono, Britantyo;Kong, Hosung;Han, Hung-Gu
    • Tribology and Lubricants
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    • v.29 no.1
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    • pp.51-55
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    • 2013
  • In this work, a new fluorescence emission measurement technology was introduced and experimentally compared with other measurement methods, such as the titration method and IR spectroscopy, to validate it for the oil oxidation measurement of electrical insulating oil. The oxidation characteristics of insulating oil were found to be fairly represented by the titration method and IR spectroscopy, and the results are comparable to a change in the fluorescence emission ratio that is defined as the shift in fluorescence intensity in the measured wavelength range. The result also shows that by the measurement of fluorescence emission ratio, it is possible to detect the oxidation of oil relatively earlier than by other methods. This study suggests that the developed technology can provide sufficient information for evaluating the insulating oil quality, and that the developed FER sensor can be used as an effective condition monitoring device of electrical insulating oil oxidation.

Optical Filter Design for Fluorescence Technique Based Phycocyanin Measurement Sensor Used In Water Treatment Plants

  • Mariappan, Vinayagam;Lee, Sung Hwa;Yang, Seungyoun;Kim, Jintae;Lee, Minwoo
    • International Journal of Internet, Broadcasting and Communication
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    • v.10 no.2
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    • pp.45-50
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    • 2018
  • Recently the water management department advised the water treatment industry to focus on deploy the chemical free and the environmentally responsible process to adopt on water treatment plants in every country. In this objective, water treatment process started using ultrasonic based phycocyanin extraction with fluorescence measurement techniques to detect the change in the yield of phycocyanin. This paper propose the design of optical filter model for fluorescence technique based immersive optical phycocyanin measurement sensor design. The proposed design uses the multi-wavelength sensor module for irradiating part, and this plays a role of removing a wavelength band other than 590 ~ 620 nm. The preliminary study on immersed phycocyanin sensor, the fluorescence value of picocyanin according to the ultrasonic intensity, treatment time and number of cells was measured using JM phycocyanin module to emulate the proposed design, and were compared performance of the proposed sensor emulation. In this design, the phycocyanin fluorescence value increased about 2.1 ~ 4.7 times as the ultrasonic treatment time increased as compared with JM phycocyanin module, and the phycocyanin fluorescence value within the analysis range was obtained by ultrasonic treatment within one minute.

Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures

  • Chattopadhyay, Saurabh;Bisaria, V.S.;Scheper, T.;Srivastava, A.K.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.6
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    • pp.331-334
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    • 2002
  • Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation In a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

An Experimental Investigation of Air Fuel Ratio Measurement using Laser Induced Acetone Fluorescence (아세톤 형광을 이용한 공연비 측정 기법 연구)

  • Park Seungjae;Huh Hwanil;Oh Seungmook
    • Proceedings of the KSME Conference
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    • 2002.08a
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    • pp.353-356
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    • 2002
  • Planar laser induced fluorescence(PLIF) has been widely used to obtain two dimensional fuel distribution. Preliminary investigation was performed to measure quantitative air excess ratio distribution in an engine fueled with LPG. It is known that fluorescence signal from acetone as a fluorescent tracer is less sensitive to oxygen quenching than other dopants. Acetone was excited by KrF excimer laser (248nm) and its fluorescence image was acquired by ICCD camera with a cut-of filter to suppress Mie scattering from the laser light. For the purpose of quantifying PLIF signal, an image processing method including the correction of laser sheet beam profile was suggested. Raw images were divided by each intensity of laser energy and profile of laser sheet beam. Inhomogeneous fluorescence images scaled with the reference data, which was taken by a calibration process, were converted to air excess ratio distribution. This investigation showed instantaneous quantitative measurement of planar air excess ratio distribution for gaseous fuel.

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Measurements of Temperature and Flow Fields with Sub-Millimeter Spatial Resolution Using Two-Color Laser Induced Fluorescence (LIF) and Micro-Particle Image Velocimetry (PIV)

  • Kim Hyun Jung
    • Journal of Mechanical Science and Technology
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    • v.19 no.2
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    • pp.716-727
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    • 2005
  • Comprehensive measurements for velocity and temperature fields have been conducted. A Micro PIV 2-color LIF system have been setup to measure the buoyancy driven fields in a 1-mm heated channel with low Grashof-Prandtl numbers [$86]. Fluorescence microscopy is combined with an MPIV system to obtain enough intensity images and clear pictures from nano-scale fluorescence particles. The spatial resolution of the Micro PIV system is $75{\mu}m\;by\;67{\mu}m$ and error due to Brownian motion is estimated $1.05\%$. Temperature measurements have achieved the $4.7\;{\mu}m$ spatial resolution with relatively large data uncertainties the present experiment. The measurement uncertainties have been decreased down to less than ${\pm}1.0^{\circ}C$ when measurement resolution is equivalent to $76\;{\mu}m$. Measured velocity and temperature fields will be compared with numerical results to examine the feasibility of development as a diagnostic technique.

Calibration-free real-time organic film thickness monitoring technique by reflected X-Ray fluorescence and compton scattering measurement

  • Park, Junghwan;Choi, Yong Suk;Kim, Junhyuck;Lee, Jeongmook;Kim, Tae Jun;Youn, Young-Sang;Lim, Sang Ho;Kim, Jong-Yun
    • Nuclear Engineering and Technology
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    • v.53 no.4
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    • pp.1297-1303
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    • 2021
  • Most thickness measurement techniques using X-ray radiation are unsuitable in field processes involving fast-moving organic films. Herein, we propose a Compton scattering X-ray radiation method, which probes the light elements in organic materials, and a new simple, non-destructive, and non-contact calibration-free real-time film thickness measurement technique by setting up a bench-top X-ray thickness measurement system simulating a field process dealing with thin flexible organic films. The use of X-ray fluorescence and Compton scattering X-ray radiation reflectance signals from films in close contact with a roller produced accurate thickness measurements. In a high-thickness range, the contribution of X-ray fluorescence is negligible, whereas that of Compton scattering is negligible in a low-thickness range. X-ray fluorescence and Compton scattering show good correlations with the organic film thickness (R2 = 0.997 and 0.999 for X-ray fluorescence and Compton scattering, respectively, in the thickness range 0-0.5 mm). Although the sensitivity of X-ray fluorescence is approximately 4.6 times higher than that of Compton scattering, Compton scattering signals are useful for thick films (e.g., thicker than ca. 1-5 mm under our present experiment conditions). Thus, successful calibration-free thickness monitoring is possible for fast-moving films, as demonstrated in our experiments.

Transverse Profile Measurement of Proton Beam using the Beam Induced Fluorescence Monitor in KIRAMS-13 Cyclotron (Beam Induced Fluorescence 모니터를 이용한 KIRAMS-13 싸이클로트론의 양성자빔 횡단면 측정)

  • Nam, S.K.;Kim, K.B.
    • Journal of the Korean Vacuum Society
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    • v.18 no.6
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    • pp.418-425
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    • 2009
  • To get the stable and optimized proton beam in the KIRAMS-13 cyclotron which installed in the regional cyclotron center, it is necessary to measure the transverse profile of proton beam. Beam Induced Fluorescence monitor is one of the non-destructive methods to measure the beam profiles, and it has many advantages such as a simple structure, real-time measurement, and minimum energy loss. The objective of this research is the design and development of Beam Induced Fluorescence monitor to measure the proton beam profiles in the KIRAMS-13 cyclotron.