• The Journal of the Korea Contents Association
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• v.15 no.5
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• pp.179-190
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• 2015

This study discusses on the influential factors of satisfaction in communication by focusing on the political communication phenomenon that is revealed in our society. The acts of political communication and its communication satisfaction are examined by exploring individuals' political inclination and their media usage which are the main influential factors of political communication. An interview survey was conducted on a thousand adults over the age of nineteen who are residing in sixteen cities and provinces nationwide. As a result, a high rate of online media usage was observed among people with progressive political inclination. It showed that the political communication acts of 'writing/communicating' which indicates ons actively posting their personal opinions, has a positive influence on communication satisfaction. On the other hand, the usage of offline media was dominant over people with conservative and neutral political inclination. A passive act of 'reading/watching,' which is to observe other people's opinions, was shown by most of the interviewees. Therefore, it was able to discuss that in order for a harmonious flow of political communication among our society, the understanding of citizens' political characteristic must be preceded and the differentiated political communication using media must be raised accordingly.

• The Journal of the Convergence on Culture Technology
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• v.9 no.5
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• pp.311-317
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• 2023

The purpose of this study is to qualitatively examine the play experience and meaning of young children who enjoy the appreciation of picture books based on the 2019 revised Nuri curriculum centered on children and play, and furthermore, various play imaginations and ideas in picture books are realized by T robot. The collected various play cases were analyzed by arranging them into play flow diagrams centering on each picture book, and based on this, the meaning of play was discovered by categorizing play through reading the meaning of play. Therefore, this study is meaningful in that it supports <Picture Book-linked Young Children's Robot Play>, a play experience in which various playful imaginations in picture books are realized by young children's robots, and examines the experience of creatively creating play led by young children in depth, and furthermore, it is valuable in providing a basis for the direction of play-centered SW education centered on young children.

• The Korean Journal of Archival Studies
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• no.41
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• pp.113-173
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• 2014

For the past few years, there has been active seeking of archival practices outside of public institutions. For example, there is oral history archive which has an actual field of its own, community archive, archives of everyday life, cultural resources archive, digital archive, and post-modern archive with its discourse practical character. In this reading, such flow is organized through everyday paradigm, and examines new archival methodology that is suitable for it. Through such critical mind, semiotic approach is taken and the need, direction and alternative of archival methodology is offered. Especially, archival methodology, which can be applied to archives is thoroughly observed. Also, the way how sign practices can be executed in the archival field is explained through specific examples. Of course, it is clearly stated that this is an instance, and that it is an archival methodology that can be applied to public institutions. We hope this would be a discuss that would enable a comprehensive understanding of records.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.51-58
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• 2002

PSI-H is an intrinsic membrane protein known to be essential for efficient electron flow in PSI complex. We isolated a cDNA clone encoding a PSI-H subunit homolog from Chinese cabbage (Brassica campestris L.). The cDNA, designated bpsaH, had an insert of 435 bp and a full open reading frame that would encode a protein of 145 amino acids. The amino acid sequence deduced from the cDNA sequence is 79.3% identical to that of spinach, suggesting the cDNA most likely encodes Chinese cabbage PSI-H subunit. The bpasH was expressed at high level in leaf tissue and low level in flower bud, whereas it was undetectable in root tissue.

• Journal of Embryo Transfer
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• v.13 no.2
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• pp.159-172
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• 1998

사람 성장호르몬 수용체(hGHR) cDNA는 PCR방법에 의하여 fagment로서 보고되어진 바 있으나, liver cDNA로 부터 전장을 cloning한 보고는 없는 실정으로 본 연구에서는 기능을 가진 약 4.6kbp의 cDNA hGHR을 cloning 하는데 성공하였다. 먼저 cloning하기 위하여 human liver mRNA와 human breast cancer tissue로부터 회수한 mRNA를 RT-PCR방법에 의하여 human cDNA library와 cloning에 필요한 probe를 제작하였다. human library mRNA는 GT-PCR방법에 의하여 증폭하여 증폭되어진 산물은 λZAP Vector를 이용하여 cDNA library를 구축하였고,screeing을 위하여 임 보고 되어진 hGHR fragment native sequence를 기초로 N-terminal부분의 primer를 설계하여 950bp의 probe를 얻는데 성공하였다. 이 probe를 이용하여 준비된 human liver cDNA library로부터 2.5$\times$10 6개의 plaque로부터 6개의 positive clone을 획득하였고, 이들중 poly Asignal인 "AATAAA"를 포함하고 있는 가장 긴 약 3.8kbp의 clone을 sequencing한 결과 open reading frame을 포함하고 있었으나, 5'부분의 결손되어 있었다. 그리하여 이 부분은 human breast cancer tissue로 부터 회수한 mRNA를 RT-PCR에 의하여 증폭하였고, sequencing결과 이미 보고되어진 native hGHR와 비교한 결과 하나의 nucleotide가 silent mutation으로 판명되었다.한편 human liver cDNA library로부터 cloning한 3.8cp의 positive clone의 5'end의 결손된 부분에 silent mutation된 PCR 산물을 연결함으로써 native hGHR와 유사한 cDNA hGHR subcloning에 성공하였다. 이러한 cDNA hGHR의 clone이 function을 가지고 있는지를 검토하기 위하여 eukaryotic 발현 vector인 pCXN2에 의거 ligation한 후 chinese hamster ovary cell[CHO-KI]에 transfect를 실시하였다. Dexamethasone은 첨가하지 않고 hGH만의 존재하에서 이들 cell을 배양시키고 cell menbrane에서 발현 여부를 판정키 위하여 hGHR monocloual antibody를 사용하여 flow cytometery해석을 실시하는 한편 125I-hGH binding assay에 의하여 hGH binding activity를 측정하였다. 최종적으로 GH signal transduction의 target genedf으로 알려져 있는 serine protease inhibitor 2.1(Spi 2.1) gene의 promotor activity를 검토한 결과 hGHR을 transfect한 CHO Cell에 있어서 hGH의 농도에 의존적으로 증가되었다. 따라서 본 실험에서 cloning한 cDNA hGHR는 native hGHR와 같은 기능을 가지는 것으로 판명되었다.것으로 판명되었다.

• The Korean Journal of Nuclear Medicine Technology
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• v.16 no.1
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• pp.50-56
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• 2012

Purpose: Those who access to the nuclear medicine department are classified as radiation workers, temporarily access group, and occasional access group as defined by the atomic energy law. The radiation workers and temporarily access people wear a personal radiation dosimeter for checking their own radiation absorbed dose periodically. However, because of the sanitation workers, classified as temporarily access group, who are working in the nuclear medicine department are moved in a cycle with other departments and their works are changeful, it is hard to control their radiation absorbed dose. Thus, this study is going to examine the state of the sanitation worker's radiation absorbed dose, and then make sure whether they are classified as temporarily access group or not. Materials and methods: In the first instance, the first sanitation worker who works in vitro laboratory and PET room and the second sanitation worker who works in gamma camera rooms (invivo room) wore radiation dosimeter-OSL(Optically Stimulated Luminescence)- to measure their own radiation absorbed dose during work time from May to June 2011. Secondly, this study was taken place 5 places in gamma camera rooms, 2 places in PET bed room, operating room, waiting room and cyclotron room in PET and 4 places in vitro laboratory. And then to measure the radiation space dose rate, it is measured 10 times each of places as sanitation worker's work flow by using radiation survey meter. Results: The radiation absorbed dose on OSL of the first c who works in vitro laboratory and PET room and the second one who works in gamma camera rooms are 0.04, 0.02 mSv per month respectively. That means the estimated annual radiation absorbed doses are less than 1mSv as 0.48, 0.24 mSv/yr respectively. The radiation space dose rates as sanitation worker's work flow using survey meter are 0.0037, 0.0019 mSv/day, so the estimated annual radiation absorbed dose are 0.93, 0.47 mSv/yr respectively. The weighted exposure dose of first sanitation worker of each places are 1.62% in cyclotron room, 3.88% in waiting room, 2.39% in operating room, 81.01% in bed room of PET and 11.01% in vitro laboratory. The weighted exposure dose of second sanitation worker of each places are 45.22% in radiopharmaceutical laboratory, gamma 30.64% in camera rooms, 15.65% in waiting room, 8.49% in reading room. Conclusion: The annual radiation absorbed doses on OSL of both sanitation workers are less than 1 mSv per year and the annual radiation absorbed doses by using survey meter are less than 1mSv either, but close up to 1 mSv. Thus, to clarify whether the sanitation workers are temporarily access group or not, and to be lessen their s radiation absorbed dose, they should be educated about management of radiation and modified their work flow or work time appropriately, their radiation absorbed dose would be lessen certainly.

• Korean Association of Arts Management
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• no.51
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• pp.135-166
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• 2019

Recently, the Japan Cultural Agency announced a plan for revitalizing the art market represented by reading museums (advanced art museums) to promote industry through strengthening the sustainability and economics of art museums. Along with these policy announcements, the Japanese cultural system and Bypyeongje are divided into pros and cons, and there has been a heightened opposition, which is now in a state where policy promotion has been temporarily suspended. The opposite reason is that it does not meet the museum's inherent purpose of preservation and lore, and the reason for favoring that commercialism can ruin the art world is that the Japanese art society is other than art museums and museums Also, it consists of non-profit organizations, art festival administration organizations, support staff, volunteers, etc., but because of the high subsidy bias, no real labor costs are paid, which means that it is virtually neglected. Also, there is a vigilance that the art society itself, which reduces its reliance on subsidies in response to social changes, can survive. Seeing that the situation is not much different from Japan, Korea is also actively discussing new establishments of the National Art Bank, performing art appraisal and evaluation functions for revitalizing art works, art loan, art trust, etc. There is. As it is difficult to solve realistic problems with subsidies from the future situation, it is difficult for us to expand investment in culture, and culture and economy are united and linked. You will find a plan to make it operational. In this regard, it is thought that the examination of the cultural and economic agency's strategy, represented by the Japanese advanced art museums, gives us a meaningful suggestion.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.15 no.1
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• pp.16-20
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• 2004

Purpose : To assess perceptual, acoustic and aerodynamic measure of voice quality in patients with unilateral vocal cord paralysis before and after type I thyroplasty. Methods : The clinical records of patients operated type I thyroplasty in the Departement of otorhinoalryngolgy, Yongdong Severance hospital from November 2001 to November 2003 were reviewed. All patients uderwent a vocal function evaluation including perceptual, acoustic and aerodynamic measures of voice preoperative and on $60^{th}$ postoperative day. The perceptual and acoustic measures were obtained from recording of patients' reading a 'Sanchak' passage. The perceptual evaluation was performed by 2 speech pathologist using a 4-point rating scale. Acoustic parameters(voice range profile low(RAL), voice range profile high(RAH), average fundamental frequency(AFX), closed quotient, harmonic to noise ratio, jitter and shimmer) were investigated by Lx speech studio. Mean flow rate(MFR), subglottic pressure(Psub) and intensity were measured using the Phonatory function analyzer. The maximum phonation time was also measured. The data were statistically analyzed. A paired t-test (p<0.1) was used to compare preoperative and postoperative results. And multiple regression test was used to find which parameter was most correlated to improvement of postoperative voice quality. Results : Among aerodynamic parameters, Psub $(88.11mmH_2O{\rightarrow}58.7mmH_2O)$, MPT(7.87sec${\rightarrow}$12.53sec), MFR (359.8ml/sec${\rightarrow}$161.06ml/sec) were statistically improved. AFx(205.5Hz${\rightarrow}$163.27Hz), AQx(23.9%${\rightarrow}$48.3%), RAL, RAH. Jotter and shimmer were improved. In multiple regression test, AFx and AQx was noted as the two meost correlated parameters to improvement of postoperative breathiness. But general grade of voice quality was more correlated to Psub and shimmer. Conclusion : Vocal fold medialization procedures effectively reduce glottic gap. Increasing of contact area of both vocal folds induced improvement in aerodynamic parameters and leaded stabilizing of vocal fold vibration. That effect results in improvement in acoustic parameters (shimmer, jitter, signal-to-noise ratio, voice range profile) and voice quality.

• Journal of the Korea Society of Computer and Information
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• v.29 no.7
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• pp.33-40
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• 2024

The live weight of livestock is important information for managing their health and housing conditions, and it can be used to determine the optimal amount of feed and the timing of shipment. In general, it takes a lot of human resources and time to weigh livestock using a scale, and it is not easy to measure each stage of growth, which prevents effective breeding methods such as feeding amount control from being applied. In this paper, we aims to improve the accuracy of weight measurement of piglets, weaned pigs, nursery pigs, and fattening pigs by collecting, analyzing, learning, and predicting video and image data in animal husbandry and pig farming. For this purpose, we trained using Pytorch, YOLO(you only look once) 5 model, and Scikit Learn library and found that the actual and prediction graphs showed a similar flow with a of RMSE(root mean square error) 0.4%. and MAPE(mean absolute percentage error) 0.2%. It can be utilized in the mammalian pig, weaning pig, nursery pig, and fattening pig sections. The accuracy is expected to be continuously improved based on variously trained image and video data and actual measured weight data. It is expected that efficient breeding management will be possible by predicting the production of pigs by part through video reading in the future.

• Journal of The Korean Association For Science Education
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• v.39 no.4
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• pp.535-544
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• 2019

In order to find out whether the traditional mass media in our society are sufficiently functioning as a vehicle of providing scientific information to the public outside the school education, public perception of science articles in mass media and scientific terms used in high frequency in science articles have been examined. To investigate the public perception on science articles, a questionnaire was constructed about the usefulness, importance, access frequency, and understanding of science articles. The questionnaires were conducted in areas with high flow populations such as train stations or subway stations. A total of 425 responses were used for analysis. In order to extract high frequency scientific terms used in science articles, two television companies and two newspapers were designated as target media, and their texts on science articles reported over the last 17 years were collected to investigate the frequency of scientific terms used. Based on the frequency, we conducted the self-report comprehension test for the top 100 scientific terms. The results of this study show that the public in our society has relatively high perception of the importance and usefulness of science articles, however, reading and understanding the articles seems to be somewhat difficult. In addition, the scientific terminology used in science articles has a high degree of comprehension for those of higher education, natural sciences majors, and men. In addition, scientific terms with high understanding degree were characterized according to gender, age, educational background, and field of major.