• Korean Journal of Plant Resources
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• v.36 no.6
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• pp.580-587
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• 2023

Androsace septentrionalis is a grass species restricted to North Korea; however, it is at the brink of extinction due to habitat loss and environmental changes caused by natural disasters and anthropogenic activities. This study was conducted to characterize the dormancy conditions of A. septentrionalis in an effort to conserve this North Korean plant resource. For this purpose, the morphological characteristics and vigor of A. septentrionalis seeds were examined, and its germination characteristics under different temperature conditions (15/6℃ and 25/15℃ ), low-temperature stratification, and gibberellin (GA₃) treatment were determined. The results revealed that A. septentrionalis exhibits non-deep type morpho-physiological dormancy, and low-temperature stratification treatment was not effective in breaking the dormancy of A. septentrionalis seeds. Meanwhile, GA₃ treatment significantly increased the mean germination time, rate, and speed of the seeds. The optimal conditions for the germination of A. septentrionalis seeds were 25/15℃ fluctuating temperature and 500 mg·L¹ GA₃ treatment. The results of this study are useful for the mass propagation of A. septentrionalis.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.39 no.1
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• pp.50-55
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• 2003

In order to study on the improvement of gill nets and trap nets fishing for the management of fisheries resource in the coastal area of Yellow Sea, we have investigated the general present condition of those fishing, and the actual fishing operation of gill nets for croaker and trap nets for rock shell which is called the the other trap nets, and then measured the body length of croaker and blue shell caught by their fishing vessels. The results are as follows ; 1. The total number of permission for coastal fishing are 12,944 cases, but the number of operation for coastal fishing are 7,558 cases in the coastal area of Yellow Sea. Among the total number of permission, the gill nets fishing are 5,154 cases with 39.8% but even so the number of operation are 3,724 cases, the trap nets fishing are 1,025 cases with 7.6% but even so the number of operation are 662 cases. On the fishing ratio, the gill nets and trap nets fishing are comparatively higher than the other fishing with 72.3% and 64.6%, respectively. 2. The main fishing period of gill nets for croaker is from the middle of July to early of September, and the main fishing grounds are sandymud bottom of 15-50m in depth around the islands of southern parts of western coastal area, and the fishing operation carry out 1∼2 times per day in flood tide and nets hauling conduct in 1∼2 hours after drifting with current. 3. The distribution range of body length of 139 croakers, which are caught in the gill nets, are 43.0∼120.0㎝ and the mode is 85.0㎝. 4. The main fishing period of trap nets for rock shell which is called the other trap nets is all the year round except the catching period of blue crab from early of September to the middle of October, and the main fishing ground are the sandymud bottom of 10∼20m in depth, and nets hauling conduct in next day after nets casting. 5. The distribution range of maximum carapace of 5,372 rock shells are 4.5∼8.5㎝ and the mode is 7.5㎝.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.263-269
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• 2023

This study aims to contribute to the research on resource recovery for the rapidly declining population of Liparis tanakae by observing the larval development process and the morphology of juveniles based on their growth. Natural spawning eggs collected in Yeosu were used for observing the process of egg development and larval morphology. The water temperature during the rearing process was maintained at 12.3~13.5℃ (average 12.7℃). The fertilized eggs had an egg diameter ranging from 1.57 to 1.79 mm (average 1.71 mm) and were spherical and adhesive. Within 4 hours 35 minutes after fertilization, they reached the two-cell stage, and after 74 hours 10 minutes, the formation of the yolk sac began. At 106 hours post-fertilization, a caudal fin appeared at the tail tip. Hatching began at 526 hours, and the larvae developed with the yolk sac positioned just behind the eyes. The newly hatched larvae had both the mouth and anus open. Melanophores appeared inside the lower jaw and around the tail on the third day after hatching. By the 16th day after hatching, most of the yolk was absorbed, and melanophores were visible in the head region. Finally, on the 63rd day after hatching, the head region significantly developed, and the body shape and mouth were similar to those of an adult fish, signifying the transition to the juvenile stage. This study will serve as valuable data for aquaculture techniques related to the conservation and restoration of fish species based on the hatching and juvenile morphology of Liparis tanakae.

• Microbiology and Biotechnology Letters
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• v.44 no.4
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• pp.479-487
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• 2016

Recently, various marine algae have been considered as a natural resource for anti-inflammation. In this research, we investigated the anti-inflammatory activity of Ulva pertusa Kjellman ethanol extract (UPKEE). This study showed that UPKEE inhibited the secretion of cytokines including IL-6, $TNF-{\alpha}$, and $IL-1{\beta}$, and reduced the expression of $NF-{\kappa}B$ and mitogen-activated protein kinases (MAPKs) as well as iNOS and COX-2. In the formation of mouse ear edema test, three doses (10, 50, 250 mg/kg body weight) of UPKEE showed inhibitory activity after inducing inflammation using croton oil. In conclusion, we found that UPKEE showed an inhibitory effect on $NF-{\kappa}B$ and MAPKs, and reduced the secretion of inflammatory cytokines. This result suggests that UPKEE can be used as a natural anti-inflammatory resource in food industry.

• Journal of Life Science
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• v.27 no.6
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• pp.652-660
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• 2017

Fermented medical herbs using Lactobacilli have attracted significant attention due to their enhanced biological activities. A traditional medicinal plant, Artemisia annua L., was fermented using a probiotic strain, L. plantarum SK3494. The strain was isolated from Artemisia princeps var. orientalis and molecularly identified through sequence similarities and phylogenetic tree analysis. The antioxidant activity of L. plantarum-fermented A. annua L. (LFA) was determined using the DPPH free radical scavenging assay. Cellular antioxidant activity of LFA was examined using the superoxide radical reduction assay in MAT-C cells. Total polyphenol contents (TPC) and flavonoid contents (TFC) of LFA were determined. The antibacterial activity of LFA against fish pathogens was also determined in this study. The viable cell number (9.38 log10 CFU/ml) and pH (4.1) results showed good adaptive ability of the selected strain during fermentation. LFA was found to have enhanced antioxidant activity compared to non-fermented A. annua L. (NFA) based on the DPPH assay. Cellular antioxidant activity was present in both LFA and NFA. After 24 hr and 48 hr of fermentation, the LFA also showed antibacterial activities against fish pathogens Photobacterium damselae subsp. damselae and Vibrio ichthyoenteri. These results suggest that L. plantarum-fermented A. annua L. may have potential as a feed additive in aquaculture.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.27 no.1
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• pp.33-47
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• 2022

In this study, distribution of submarine sand ridges in the coastal waters of Korea was surveyed using multibeam echo sounder data, and the topographic characteristics of each region were identified. For this purpose, the DEM (Digital Elevation Model) data was generated using depth data obtained from the Yellow Sea and the South Sea by Korea Hydrographic and Oceanographic Agency, and then applied the TPI (Topographic Position Index) technique to precisely extract the boundary of the sand ridges. As a result, a total of 200 sand ridges distributed in the coastal waters were identified, and the characteristics of each region of the sedimentary sediments were analyzed by performing statistical analysis on the scale (width, length, perimeter, area, height) and shape (width/length ratio, height/width ratio, linear·branch type, exposure·non-exposure type). The results of this study are expected to be used not only for coastal navigational safety, but also for marine naming support, marine aggregate resource identification, and fisheries resource management.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.07a
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• pp.51-51
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• 2005

We inestigated the reproductive cycle with ovarian development of Chlamys farreri by histological observations, and seasonal changes in biochemical components of the adductor muscle, digestive diverticula and ovary were studied by biochemical analysis. The reproductive cycle of this species can be classified into five successive stages: early active stage (January to March), late active stage (March to April), ripe stage (April to August), partially spawned stage (June to August) and spent/inactive stage (August to January). According to ANOVA test, there were significant differences (p<0.05) in total protein, total lipid and glycogen contents among months for all of the adductor muscle, digestive diverticula and ovary. Total protein contents in ovary and digestive diverticula showed significant changes(ANOVA, p<0.05) during the study period, while that in the adductor muscle did not. Total protein content was highest in the adductor muscle, followed by ovary, and lowest in digestive diverticula. There was no correlation in total protein content between the adductor muscle and digestive diverticula (p=0.220). But strong positive correlation was found between adductor muscle and ovary (r=0.450, p=0.013). ANOVA showed that there were significant differences in total lipid and glycogen contents among months for all of the adductor muscle, ovary, and digestive diverticula (p<0.05). The monthly changes in total lipid content were highly variable in ovary and digestive gland. High contents of total lipid were foung during April and May-June in ovary, while March and June-July in digestive diverticula. There was a strong negative correlation in total lipid content between ovary and digestive diverticula (r=- 0.397, p=0.030). Unlike total protein of total lipid, glycogen content in the adductor muscle was most dynamic. It showed more than 36-fold changes in the adductor muscle (at most 3-fold change in ovary) during the study period. Glycogen content was higher during May-July in the adductor muscle, While it was higher in March and august in digestive diverticula. There was a strong negative correlation in glycogen content between the adductor muscle and digestive diverticula (r=-0.584, p=0.001).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.5
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• pp.483-487
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• 2001

As a part of basic investigation for utilizing of cuttle bone as a calcium source, we examined on the extraction methods (calcining at $800^{\circ}C$ for 2 hrs, autoclaving at $121^{\circ}C$ for 10 hrs and ultrasonic treatments at $60^{\circ}C$ for 10 hrs) as a pretreatment methods for preparation of calcium-based powder from cuttle bone, The color of calcined calcium-based powder from cuttle bone was white, while that treated by other methods was light yellow. The calcium content in calcined calcium-based powder was $70.5\%$, and revealed high about 2 times compared to those pretreated by other methods. And, calcium solubility in calcined calcium-based powder was improved 22 times compared to raw cuttle bone powder, Among calcining, autoclaving and ultrasonic treatments as a pretreaoent methods for preparation of calcium-based powder from cuttle bone, calcining treatment was superior to other methods on the aspect of color, calcium solubility and purity of calcium-based powder, Judging from X-ray diffraction pattern of calcined calcium-based powder, most of calcium was present as a form of calcium oxide, But, pH of calcined calcium-based powder revealed strong alkali of pH 12.9. This pH value might invoke health risk in using food resource. Therefore, for utilization effectively calcined calcium-based powder from cuttle bone, it requires a suitable treatment such as adjustment of pH to neutral condition.

• Proceedings of KOSOMES biannual meeting
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• 2009.06a
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• pp.105-111
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• 2009

Marine pollution prevention manager system has been operated for the purpose of preventing marine pollution from ships and marine facilities in Korea. As a new "marine environment management act" replacing an old "marine pollution prevention act" had entered into force from January 20, 2008, the works and business of the education and training courses for marine pollution prevention manager are scheduled to transfer from KIMFT(Korea Institute of Maritime and Fisheries Technology) and FHRDI(Fisheries Human Resource Development Institute) to KOEM(Korea Marine Environment Management Corporation) on January 1, 2011. The present state of the education and training for marine pollution prevention managers on board ships was surveyed and analyzed In order to prospect trainee demands for the education and training and to provide KOEM with basic data for establishing a new system for the education and training. The existing education and training courses of KIMFT for marine pollution prevention manager on board ships are classified into a regular course of 21 classes for 3 days, a reeducation course of 18 classes for 2 days and a NLS course of 17 classes for 2days. The annual numbers of trainees in 2008 were 516 persons for regular course, 1085 persons for reeducation course and 135 persons for NLS course in KIMFT. The trainee demands for the education and training were attempted to be predicted on a basis on annual increase rate of ships. The predicted demands for the education and training are changeable depending on the major factors such shipping market, naval architecture industry, etc.

• Proceedings of KOSOMES biannual meeting
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• 2009.06a
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• pp.113-119
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• 2009

Marine pollution prevention manager system has been operated for the purpose of preventing marine pollution from ships and marine facilities in Korea. As a new "marine environment management act" replacing an old "marine pollution prevention act" had entered into force from January 20. 2008. the works and business of the education and training courses for marine pollution prevention manager are scheduled to transfer from KIMFT(Korea Institute of Maritime and Fisheries Technology) and FHRDI(Fisheries Human Resource Development Institute) to KOEM(Korea Marine Environment Management Corporation) on January 1. 2011. The present state of the education and training for marine pollution prevention managers of marine facilities was surveyed and analyzed in order to prospect trainee demands for the education and training and to provide KOEM with basic data for establishing a new system for the education and training. The existing education and training courses of FHRDI for marine pollution prevention manager of marine facilities have been conducted as a regular course of 21 classes for 3 days. The average annual number of trainees in FHRDI for 6 years from 2003 to 2008 was 187 persons and the annual number of trainee was 202 persons in 2008. The trained demands for the education and training are expected to increase sooner or later, depending major factors such as the extension of fields and ranges for marine facilities and marine environment management business due to the enforcement of new "marine environment management act".