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양어 사료첨가제로서의 유산균 발효 개똥쑥의 항산화 및 항균활성

Antioxidant and Antibacterial Activities of Lactobacillus-fermented Artemisia annua L. as a Potential Fish Feed Additive

  • 이아란 (건국대학교 동물자원과학과) ;
  • 우개민 (건국대학교 동물자원과학과) ;
  • 강수경 (건국대학교 축산식품공학과) ;
  • 한성구 (건국대학교 축산식품공학과) ;
  • 이봉주 (국립수산과학원 사료연구센터) ;
  • 김수기 (건국대학교 동물자원과학과)
  • Lee, Ah-Ran (Department of Animal Science and Technology, Konkuk University) ;
  • Niu, Kai-Min (Department of Animal Science and Technology, Konkuk University) ;
  • Kang, Su-Kyung (Department of Food Science of Animal Resource, Konkuk University) ;
  • Han, Sung-Gu (Department of Food Science of Animal Resource, Konkuk University) ;
  • Lee, Bong-Joo (Aquafeed Research Center, National Institute of Fisheries Science) ;
  • Kim, Soo-Ki (Department of Animal Science and Technology, Konkuk University)
  • 투고 : 2017.01.02
  • 심사 : 2017.02.15
  • 발행 : 2017.06.30

초록

최근 양어에서는 전염병, 환경오염, 어분 가격 상승 등으로 어려움을 가지고 있다. 본 연구는 쑥에서 분리한 L. plantarum SK3494를 이용하여 전통 약초인 개똥쑥을 발효시켜 in vitro 생리활성을 측정하고 어류용 사료첨가제로서의 가능성을 연구하였다. 개똥쑥의 발효시 유산균수는 9.38 log10 CFU/ml이며, pH는 4.1로 나타났다. 발효개똥쑥의 항산화활성은 DPPH 측정과 MAC-T 세포 내 과산화물 감소 실험에서 뛰어난 효과를 보였다. 발효개똥쑥은 어류 병원균인Photobacterium damselae subsp. damselae와 Vibrio ichthyoenteri에 대하여 강한 항균활성을 나타내었다. 따라서 유산균을 이용한 개똥쑥 발효물은 양식용 사료첨가제로써 가능성을 가지고 있는 것으로 판단되었다.

Fermented medical herbs using Lactobacilli have attracted significant attention due to their enhanced biological activities. A traditional medicinal plant, Artemisia annua L., was fermented using a probiotic strain, L. plantarum SK3494. The strain was isolated from Artemisia princeps var. orientalis and molecularly identified through sequence similarities and phylogenetic tree analysis. The antioxidant activity of L. plantarum-fermented A. annua L. (LFA) was determined using the DPPH free radical scavenging assay. Cellular antioxidant activity of LFA was examined using the superoxide radical reduction assay in MAT-C cells. Total polyphenol contents (TPC) and flavonoid contents (TFC) of LFA were determined. The antibacterial activity of LFA against fish pathogens was also determined in this study. The viable cell number (9.38 log10 CFU/ml) and pH (4.1) results showed good adaptive ability of the selected strain during fermentation. LFA was found to have enhanced antioxidant activity compared to non-fermented A. annua L. (NFA) based on the DPPH assay. Cellular antioxidant activity was present in both LFA and NFA. After 24 hr and 48 hr of fermentation, the LFA also showed antibacterial activities against fish pathogens Photobacterium damselae subsp. damselae and Vibrio ichthyoenteri. These results suggest that L. plantarum-fermented A. annua L. may have potential as a feed additive in aquaculture.

키워드

참고문헌

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