Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-Inflammatory Effects of Ethanol Extracts from Ulva pertusa Kjellman on LPS-induced RAW 264.7 Cells and Mouse Model

LPS로 유발한 대식세포의 염증반응과 마우스 귀 부종에 대한 구멍갈파래 에탄올 추출물의 항염증 효과

  • Kim, Min-Ji (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Min-Ju (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Choi, Hyeun-Deok (Institute of Fisheries Sciences, Pukyong National University) ;
  • Park, So-Yeong (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Jang, Mi-Ran (Health Functional Food Policy Division, Ministry of Food and Drug Safety) ;
  • Im, Moo-Hyeog (Department of Food Science and Biotechnology, Daegu University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 김민주 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 박선희 (부경대학교 식품공학과/식품연구소) ;
  • 최현덕 (부경대학교 수산과학연구소) ;
  • 박소영 (부경대학교 식품공학과/식품연구소) ;
  • 장미란 (식품의약품안전처 건강기능식품정책과) ;
  • 임무혁 (대구대학교 식품공학과) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2016.09.19
  • Accepted : 2016.11.24
  • Published : 2016.12.28
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, various marine algae have been considered as a natural resource for anti-inflammation. In this research, we investigated the anti-inflammatory activity of Ulva pertusa Kjellman ethanol extract (UPKEE). This study showed that UPKEE inhibited the secretion of cytokines including IL-6, $TNF-{\alpha}$, and $IL-1{\beta}$, and reduced the expression of $NF-{\kappa}B$ and mitogen-activated protein kinases (MAPKs) as well as iNOS and COX-2. In the formation of mouse ear edema test, three doses (10, 50, 250 mg/kg body weight) of UPKEE showed inhibitory activity after inducing inflammation using croton oil. In conclusion, we found that UPKEE showed an inhibitory effect on $NF-{\kappa}B$ and MAPKs, and reduced the secretion of inflammatory cytokines. This result suggests that UPKEE can be used as a natural anti-inflammatory resource in food industry.

대식세포에 대하여 구멍갈파래 에탄올 추출물의 독성결과를 확인해본 결과 독성은 나타나지 않았으며, LPS에 의하여 유도되는 NO와 염증성 cytokine의 분비량은 구멍갈파래 에탄올 추출물의 농도 의존적으로 감소함을 확인하였다. 또한 구멍갈파래 에탄올 추출물로 인해 $NF-{\kappa}B$ 및 MAPKs의 신호전달을 억제함으로써 염증매개성 물질의 발현 억제에 효과가 있는지 알아본 결과, 구멍갈파래 에탄올 추출물은 각각 iNOS, COX-2, $NF-{\kappa}B$ 및 MAPKs의 활성을 효과적으로 억제하였고 그에 따른 염증 매개인자들의 생성도 효과적으로 억제되는 것을 확인하였다. 마지막으로 추출물이 마우스 귀부종에 미치는 영향을 살펴본 결과, 대조군의 경피와 진피의 두께에 비해 추출물 처리군의 조직 두께가 상대적으로 현저히 줄어들었으며 귀 조직에 침윤된 mast cell의 감소에도 추출물이 그 효과를 현저하게 나타냄을 확인하였다. 본 연구결과들을 종합해 보았을 때, 구멍갈파래의 에탄올 추출물은 항염증 활성을 가지는 새로운 천연물질로 이용 가능하여 고부가 가치 제품 개발이 가능한 천연 소재로 판단된다.

Keywords

References

  1. Ayako Y, Koichi Y, Keiichi O. 1999. Iodine distribution in blades of several laminarias grown in the same sea area. Bull. Jpn. Soc. Sci. Fish. 58: 1373-1379.
  2. Azuine MA, Goswami UC, Katal JJ. 1992. Antimutagenic and anticarcinogenic effect of carotenoids and dietary palm oil. Nutr. Cancer. 17: 287-295. https://doi.org/10.1080/01635589209514198
  3. Bae NY, Kim MJ, Kim KBWR, Ahn NK, Choi YU, Park JH, et al. 2015. Anti-inflammatory effect of ethanol extract from Grateloupia elliptica Holmes on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells and mice ears. J. Korean Soc. Food Sci. Nutr. 44: 1128-1136. https://doi.org/10.3746/jkfn.2015.44.8.1128
  4. Cho BO, Yin HH, Fang CZ, Ha HO, Kim SJ, Jeong Sl, et al. 2015. Synergistic anti-inflammatory effect of rosmarinic acid and luteolin in lipopolysaccharide-stimulated RAW264.7 macrophage cells. Korean J. Food Sci. Technol. 47: 119-125. https://doi.org/10.9721/KJFST.2015.47.1.119
  5. Qin D, Zhou YI, Zhang SZ, Cao JM, Xu LY, Fang GD, et al. 2015. Anti-inflammation of Tripterygium wilfordii polycoride on macrophages and its regulation to inflammation via TLR4/$NF-{\kappa}B$. CHM 7: 155-161.
  6. Eum WS, Lee KJ, Kim DW, Lim SS, Kang IJ, Park J, et al. 2013. Anti-inflammatory effects of extracts from Caesalpinia sappan L. on skin inflammation. J. Korean Soc. Food Sci. Nutr. 42: 1513-1517. https://doi.org/10.3746/jkfn.2013.42.9.1513
  7. Jeong DH, Kim KBWR, Kim MJ, Kang BK, Ahn DH. 2014. Antiinflammatory activity of methanol extract and nhexane fraction mojabanchromanol b from Myagropsis myagroides. Life Sci. 114: 12-19. https://doi.org/10.1016/j.lfs.2014.07.036
  8. Ji JD. 2005. Cytokines in rheumatoid arthritis. Hanyang Med. Rev. 25: 43-52.
  9. Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Kim BR, et al. 2014. Anti-inflammatory activity of an ethanol extract of Laminaria japonica root on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells. Korean J. Food Sci. Technol. 46: 729-733. https://doi.org/10.9721/KJFST.2014.46.6.729
  10. Kim MJ, Bae NY, Kim KBWR, Park JH, Park SH, Cho YJ, et al. 2015. Anti-inflammatory effect of Zostera marina ethanolic extract on LPS induced RAW264.7 cells and mouse model. KSBB J. 30: 182-190. https://doi.org/10.7841/ksbbj.2015.30.4.182
  11. Kim MJ, Bae NY, Kim KBWR, Park JH, Park SH, Choi JS, et al. 2016. Anti-inflammatory effect of Grateloupia imbricata holmes ethanol extract on LPS-Induced RAW 264.7 cells. J. Korean Soc. Food Sci. Nutr. 5: 181-187.
  12. Kim MJ, Bea NY, Kim KBWR, Park JH, Park SH, Cho YJ, et al. 2015. Anti-inflammatory effect of water extract from tuna heart on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells. Korean Soc. Biotechnol. Bioeng. J. 30: 326-331.
  13. Kim MJ, Kim KBWR, Jeong DH, Ahn DH. 2013. Anti-inflammatory activity of ethanolic extract of Sargassum sagamianum in RAW 264.7 cells. Food Sci. Biotechnol. 22: 1113-1120. https://doi.org/10.1007/s10068-013-0191-9
  14. Kim MJ, Bae NY, Kim KBWR, Park JH, Park SH, Jang MR, et al. 2016. Anti-inflammatory effect of Chondrus nipponicus Yendo ethanol extract on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells. J. Korean Soc. Food Sci. Nutr. 45: 194-201. https://doi.org/10.3746/jkfn.2016.45.2.194
  15. Lee HN, Lim DY, Lim SS, Kim JD, Park JHY. 2011. Anti-inflammatory effect of ethanol extract from Eupatorium japonicum. Korean J. Food Sci. Technol. 43: 65-71. https://doi.org/10.9721/KJFST.2011.43.1.065
  16. Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW, Jo SK. 2000. Induction of nitric oxide and $TNF-{\alpha}$ by herbal plant extracts in mouse macrophages. J. Korean Soc. Food Sci Nutr. 29: 342-348.
  17. Margret RJ, Kumaresan S, Ravikumar S. 2009. A preliminary study on the anti-inflammatory activity of methanol extract of Ulva lactuca in rat. J. Environ. Biol. 30: 899-902.
  18. Marks-Konczalk J, Chu SC, Moss J. 1998. Cytokine-mediated transcriptional induction of the human inducible nitiric oxide synthase gene requires both activator protein and nuclear factor kapaB-binding sites. J. Biol. Chem. 273: 22201-22208. https://doi.org/10.1074/jbc.273.35.22201
  19. Miwa K, Kambara H, Kawai C. 1983. Effect of aspirinin large doses on attacks of variant angina. Am. Heart J. 105: 351-355. https://doi.org/10.1016/0002-8703(83)90548-3
  20. Nathan C. 1992. Nitric oxide as a secretory product of mammalian cells. FASEB J. 6: 3051-3064. https://doi.org/10.1096/fasebj.6.12.1381691
  21. Park JH, Kwon DY, Lee SK. 2014. Effects of Hoesaeng-san ethanol extract on the human mast cell-mediated inflammatory responses. Korean J. Oriental Physiol. Pathol. 28: 45-52.
  22. Ray B, Lahye M. 1995. Cell-wall polysaccharides from the marine green alga Ulva rigida (Ulvaes, chlorphyta), extraction and chemical composition. Carbohydr. Res. 274: 251-261. https://doi.org/10.1016/0008-6215(95)00138-J
  23. Shon DH, Choi DW, Kim MH. 2012. Improvement of anti-inflammation activity of Gardeniae fructus extract by the treatment of $\beta$-glucosidase. Korean J. Food Sci. Technol. 44: 331-336. https://doi.org/10.9721/KJFST.2012.44.3.331
  24. Tete S, Tripodi D, Rosati M, Conti F, Maccauro G, Saggini A, et al. 2008. The role of mast cells in innate and adaptive immunity. Life Sci. J. 18: 891-896. https://doi.org/10.5352/JLS.2008.18.6.891
  25. Yoo JS, Cheun BS, Kim NG. 2001. Determination of $Na^+$ channel blocker in seaweeds. Korean J. Environ. Biol. 19: 107-112.