• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.4
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• pp.42-51
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• 2010

The purpose of this study is to analyze the characteristics of environment-friendliness of Root Barrier materials based on comprehensive experiments on harmfulness of Root Barrier materials and fish toxicity of Oryzias latipes mortality to verify eco-toxicity of each method of construction and Root Barrier material, which are to be applied by taking eco-toxicity into account when building ecological flows in upper areas on natural and artificial grounds. As a result, the following conclusions have been reached in this study: 1. In regard of the harmfulness analyzed, each material showed a different result of analytical value in each lab tank. Compared to lab tank, pH (la), DO (lb), T-N (VI) and T-coli (la) were in the same grade, but COD, SS, T-P and F-coli were less than that of control, respectively increased or decreased by material were analyzed. 2. In the experiment of fish toxicity, Barrier sheet was found to have 66.7% of fish mortality, indicating strong fish toxicity. Synthetic rubber system root barrier sheet (20.0%) was analyzed to have medium fish toxicity, while Synthetic resin system root barrier-waterproof sheet (3.3%), Synthetic rubber system membrane root barrier sheet (3.3%) and Synthetic resin system root barrier sheet (0.0%) showed relatively lower mortality and fish toxicity. To sum up such results as found in the experiment mentioned so far, the values of harmfulness and root penetration resistance analyzed were different in each lab tank, but there was absolutely little correlation with the mortality gained from the experiment on fish toxicity. In the experiment of fish toxicity, environment-friendly root barrier materials were analyzed, and it was found that Synthetic resin system root barrier sheet, Synthetic resin system root barrier waterproof sheet and Synthetic rubber system membrane root barrier sheet are highly environment-friendly. In contrast, Synthetic rubber system root barrier sheet was found to have medium-level environment-friendliness. Also, Barrier sheet was analyzed to have low environment-friendliness.

• Fisheries and Aquatic Sciences
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• v.22 no.5
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• pp.10.1-10.14
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• 2019

Fish skin waste accounts for part of the solid waste generated from seafood processing. Utilization of fish skin by bioconversion into high-grade products would potentially reduce pollution and economic cost associated with treating fish processing waste. Fish skin is an abundant supply of gelatin and collagen which can be hydrolyzed to produce bioactive peptides of 2-20 amino acid sequences. Bioactivity of peptides purified from fish skin includes a range of activities such as antihypertensive, anti-oxidative, antimicrobial, neuroprotection, antihyperglycemic, and anti-aging. Fish skin acts as a physical barrier and chemical barrier through antimicrobial peptide innate immune action and other functional peptides. Small peptides have been demonstrated to possess biological activities which are based on their amino acid composition and sequence. Fish skin-derived peptides contain a high content of hydrophobic amino acids which contribute to the antioxidant and angiotensin-converting enzyme inhibitory activity. The peptide-specific composition and sequence discussed in this review can be potentially utilized in the development of pharmaceutical and nutraceutical products.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.9
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• pp.76-83
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• 2017

Fish respond sensitively to light, so it is possible to develop fish management technology using this feature. In this study, we developed a light-based fish barrier and analyzed itsblocking effect using the difference in illuminance for the major fish species in Korea, bass and bluegill. The light was generated by a light emitting diode and the facility was installed vertically from the bottom. Considering the fish's ability to travel upstream, the flow rate was divided into three stages (0.2, 0.1, and 0.05 m/s). To prevent the learning effect, an experiment was carried out with fish that had rested for more than one day in a rearing tank. The experiment was carried out in such a way as tocompare the number of fish which travelled upstream after the introductionof the fish barrier and that of the fish which travelled upstream after itsremoval. It was also carried out after sunset to increase the effectiveness of the barrier. According to the results of the experiment, the fish blocking effect depending on the difference in illuminance was high and, overall, the blocking rate for bass was lower than that for bluegill. Based on the total size of the experimental population, the blocking rates for bass and bluegill were 96.33% and 99.00%, respectively. Based on the number of fish that travelled upstream, the blocking rates for bass and bluegill were 91.73% and 98.73%, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.55 no.6
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• pp.910-916
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• 2022

The purpose of this study is to evaluate the effect of underwater non-thermal dielectric barrier discharge plasma (DBD plasma) on the sterilization of three types of pathogenic bacteria that cause diseases in freshwater fish and the reduction of a tetracycline antibiotics. This experiment was conducted in the DBD plasma generator, and the voltages used to generate plasma were 11.6 kV and 23.1 kV. The measurement intervals were 0, 1, 5, 10 and 15 min. As a result of DBD plasma treatment, Aeromonas hydrophila, Edwardsiella tarda and Pseudomonas fluorescens were removed 93-99% after 5 min at 23.1 kV, and the tetracycline antibiotics were reduced 70-95% after 15 min at 23.1 kV. In this study, as a result of treating the effluent with DBD plasma at a fish farm where the medicinal bath was conducted with oxytetracycline-HCl (OTC-HCl) products, OTC-HCl decreased by 62% after 10 min at 23.1 kV.

• Journal of fish pathology
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• v.5 no.2
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• pp.159-164
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• 1992

The skin of fishes is also the important external barrier against a variety of the environmental detrimental agents, allowing normal internal physiological function, so its condition is very important in many disease processes. Since fishes are aquatic, morphologically and physiologically they posses specific characteristics lacking in terrestial animals. Thus, it is necessary to keep in mind various specific histologic characteristics for a precise interpretation of the histopathological lesions. In the normal environment the fish skin is more subtly adapted for its physiological requirements as a limiting barrier than the skin of higher terrestial vertebrates. However, its delicacy makes it high vulnerable to damage in fish culture facilities or polluted waters. Although changes in the skin of fish such as discoloration, ulcerative lesions, are the the most readily observed clinical features of fish, there is a wide variety of possible responses, which are best considered in terms of their site within the skin. Up to now, there are relatively few sytematically described information on the histological structure of the teleost skin as well as integrated reviews of the range of pathological processes that can occur in teleost skin. Therefore it was tried in this article to rearrange the information already described by other investigators on the histological structure and histopathological changes of teleost skin.

• Applied Chemistry for Engineering
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• v.25 no.2
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• pp.174-180
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• 2014

A plasma hydrophobic coating on commercial fish feed was conducted to prolong the floating time of feed, thereby enhancing the feed consumption rate and reducing the contamination of water in fish farms. The hydrophobic coating on the fish feed was prepared using an atmospheric-pressure dielectric barrier discharge (DBD) plasma with hexamethyldisiloxane (HMDSO), toluene and n-hexane as the precursors. The effect of the parameters such as input power, precursor type and coating time on the coating performance were examined. The physicochemical properties of the coating layer were analyzed using a Fourier transform infrared (FTIR) spectrometer and a contact angle (CA) analyzer. The water CA increased after the coating preparation, indicating that the surface changed from hydrophilic to hydrophobic. The FTIR characterization revealed that the hydrophobic layer was comprised of functional groups such as $CH_3$, Si-O-Si and Si-C. As a result of the hydrophobic coating, the floating time of the fish feed increased from several seconds to 3 minutes, which suggested that the plasma coating method could be a viable means for practical applications. Compared to the water CA measured as soon as the coating layer was prepared, the 6-day aged sample exhibited a substantial CA increase, confirming the aging effect on the improvement of the hydrophobicity.

• Journal of Environmental Impact Assessment
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• v.21 no.6
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• pp.859-877
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• 2012

In this study, Echo-sounder($E_s$) monitoring methodology was applied to test environmental impacts of artificial weirs(SCW and JSW) in the Yeongsan River on fish community and habitat structures at first time, and was compared with conventional fish sampling methodologies($C_s$). For the Es monitoring in the fields, parallel transect methods was employed in determining the survey distance interval with every 125 m within the upper-lower 2 km of the weirs. Four different fish sampling gears such as casting nets, kick nets, fyke nets, and gill nets were used for applications of $C_s$ monitoring methodology. According to the Echo-sounder monitoring, fish density, expressed as a number of individuals per square meter, along the longitudinal axis of the weir was significantly greater(JSW, t = 3.506, n = 30, p < 0.001) in the down-river reach of the weir than in the up-river reach. Also, fish density along the vertical water column was highest at mid-depths of Seungchon weir, which has simple habitat substrates, while fish density was highest at hypolimnetic depth of Juksan weir. According to fish sampling by the $C_s$ methodology, the fish compositions decreased as the river goes upward, and significant differences(JSW, t = 0.248, n = 30, p < 0.05) in the compositions of fish species occurred between up-reach and down-reach of the weirs. The dominant species near the two weirs were Opsarichthys uncirostris amurensis, Hemiculter eigenmanni and Coilia nasus. Overall, our fish and habitat data, based on $E_s$ and $C_s$ monitoring methodologies, suggest that the weirs disturbed the rivers due to initial habitat disturbances by the weir constructions as well as the barrier roles of weirs on fish passage and migrations. More long-term scientific and systematic fish impact monitoring and assessments($E_s$ and $C_s$) are required in the future to predict changes of ecological structures and functions on the constructions of the weirs.

• Korean Journal of Environmental Biology
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• v.40 no.4
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• pp.641-650
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• 2022

The purpose of this study was to compare the efficiency of air and oxygen injected into the underwater non-thermal dielectric barrier discharge plasma (DBD plasma) device used to remove five types of antibiotics (tetracycline, doxycycline, oxytetracycline, clindamycin, and erythromycin) artificially contained in the fish farm discharge water. The voltage given to generate DBD plasma was 27.8 kV, and the measurement intervals were 0, 0.5, 1, 2, 4, 8, 16 and 32 minutes. Tetracycline antibiotics significantly decreased in 4 minutes when air was injected and were reduced in 30 seconds when oxygen was injected. After the introduction of air and oxygen at 32 minutes, 78.1% and 95.8% of tetracycline were removed, 77.1% and 96.3% of doxycycline were removed, and 77.1% and 95.5% of oxytetracycline were removed, respectively. In air and oxygen, 59.6% and 83.0% of clindamycin and 53.3% and 74.3% of erythromycin were removed, respectively. The two antibiotics showed lower removal efficiency than tetracyclines. In conclusion, fish farm discharge water contains five different types of antibiotics that can be reduced using underwater DBD plasma, and oxygen gas injection outperformed air in terms of removal efficiency.

• Journal of Korean Society of Forest Science
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• v.108 no.2
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• pp.224-232
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• 2019

The movement of fish to the upper and the lower parts of a mountain stream was investigated based on the installation of specific types of structures within the stream. The results indicated that as the flow rate increases after a rain, the height of the drop in an open-type structure, such as a concrete box, is decreased so that the floating fishes, such as the Chinese minnow fish, move relatively easily from the upper to the lower parts and from the lower to the upper parts of the stream. In contrast, the fishes released from an upper point of a wall-type structure with <1 m, such as rocks drop works and stone masonry for stream-grade stabilization, were trapped in the lower part of the stream and, even after the rainy season, it was difficult for the fish to move from the lower to the upper parts of the stream. In particular, the barrier-type structures ${\geq}3m$ limit the movement of fish, even when there are few drainage holes at the lower end of the dam, and there is no space for the fishes to communicate with each other, even when the flow rate is high; therefore, although the fish are active, they are restricted to move according to the type and characteristics of the structures. When installing structures in a mountain stream, the height of the structure must be low enough to allow the fish to communicate with each other or an open-type structure must be installed. The fish habitat and water conditions within the stream must be considered when designing the type and height of the in-stream structures.

• Journal of Ecology and Environment
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• v.48 no.1
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• pp.32-48
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• 2024

Background: Longitudinal connectivity in river systems strongly affects biological components related to ecosystem functioning, thereby playing an important role in shaping local biodiversity and ecosystem health. Environmental DNA (eDNA)-based metabarcoding has an advantage of enabling to sensitively diagnose the presence/absence of species, becoming an efficient/effective approach for studying the community structure of ecosystems. However, little attention has been paid to eDNA-based biomonitoring for river systems, particularly for assessing the river longitudinal connectivity. In this study, by using eDNA we analyzed and compared species diversity and composition among artificial barriers to assess the longitudinal connectivity of the fish community along down-, mid- and upstream in the Hotancheon from the Geum River basin. Moreover, we investigated temporal variation in eDNA fish community structure and species diversity according to season. Results: The results of species detected between eDNA and conventional surveys revealed higher sensitivity for eDNA and 61% of species (23/38) detected in both methods. The results showed that eDNA-based fish community structure differs from down-, mid- and upstream, and species diversity decreased from down to upstream regardless of season. We found that there was generally higher species diversity at the study sites in spring (a total number of species across the sites [n] = 29) than in autumn (n = 27). Nonmetric multidimensional scaling and heatmap analyses further suggest that there was a tendency for community clusters to form in the down-, mid- and upstream, and seasonal variation in the community structure also existed for the sites. Dominant species in the Hotancheon was Rhynchocypris oxycephalus (26.07%) regardless of season, and subdominant species was Nipponocypris koreanus (16.50%) in spring and Odontobutis platycephala (15.73%) in autumn. Artificial barriers appeared to negatively affect the connectivity of some fish species of high mobility. Conclusions: This study attempts to establish a biological monitoring system by highlighting the versatility and power of eDNA metabarcoding in monitoring native fish community and further evaluating the longitudinal connectivity of river ecosystems. The results of this study suggest that eDNA can be applied to identify fish community structure and species diversity in river systems, although some shortcomings remain still need to be resolved.