• 한국산림과학회지
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• 제98권5호
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• pp.558-562
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• 2009

The growth, carbon and nitrogen status of container grown black pine (Pinus thunbergii) seedlings were examined at various levels of foliar fertilization (control, 0.1%, 0.2%, 0.3%). Root collar diameter, height and dry weight of black pine seedlings increased significantly with increasing levels of foliar fertilization (P<0.05). Carbon concentration in needle of black pine seedlings was significantly higher in the foliar fertilization than in the control treatments (P<0.05), while other seedling components such as stem and roots were not significantly different (P>0.05) between the foliar fertilization and the control treatments. Nitrogen concentration and content were significantly greater in the foliar fertilization than in the control treatments (P<0.05). Shoot/root ratio of black pine seedlings (needle+stem dry weight/root dry weight) was greater in the foliar fertilization (2.40-2.89) than in the control treatments (1.87). However, nitrogen use efficiency was significantly lower (P<0.05) in the foliar fertilization (28-46) than in the control (111) treatments. The results indicate that morphological characteristics and nutritional status on container grown black pine seedlings were enhanced by various levels of foliar fertilization.

• Clinical and Experimental Reproductive Medicine
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• 제27권2호
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• pp.191-200
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• 2000

Objective: This study was carried out to compare the effects of the stepwise exposure treatments on the morphological normality, fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing and to use as a fundamental data for the cryopreservation of human oocytes. Materials and Methods: The morphological normality and fertilization rates of the vitrified and ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were observed. After choosing the 3step exposure treatment groups, we observed the morphological normality and fertilization, blastocyst formation rate of the vitrified and ultra-rapid frozen mouse mature oocytes. Results: The morphological normality and fertilization rates of the vitrified mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 75%, 85%, 88% and 58%, 61 %, 54% respectively. There were no significant differences among treatments(p>0.05). The morphological normality and fertilization rate of the control was 92% and 65%. There were no significant differences in fertilization rate among control and treatments (p>0.05). The morphological normality and fertilization rates of the ultra-rapid frozen mouse mature oocytes after three-stepwise exposure treatments (1step, 3step and 5step) were 83%, 83%, 84% and 75%, 63%, 56% respectively. There were no significant differences among treatments (p>0.05). The morphological normality and fertilization rate of the control was 95% and 67%. There were no significant differences among control and treatments (p>0.05). The morphological normality and fertilization rate of the vitrified or ultra-rapid frozen mouse mature oocytes after 3step exposure treatment were 69% and 75%, respectively. The blastocyst formation rate was 60% and 57%. The results did not differ significantly between vitrification and ultra-rapid freezing (p>0.05). Conclusion: As known in the above results, there were no significant differences in the fertilization and blastocyst formation rate of the frozen-thawed mouse mature oocytes by vitrification or ultra-rapid freezing among the control and treatments. It is suggested that vitrification and ultra-rapid freezing method were effective for the cryopreservation of mouse mature oocytes.

• Journal of Ecology and Environment
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• 제29권2호
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• pp.151-155
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• 2006

Root nodule biomass, and seedling biomass and growth were examined for 2-year-old Robinia pseudoacacia and Amorpha fruticosa seedlings following fertilization treatments. Organic fertilizer, solid combination fertilizer, and organic fertilizer plus solid combination fertilizer were used for the study. Root nodule biomass (g/plant) ranged from 3.00 to 7.06 for R. pseudoacacia and varied from 1.52 to 2.32 for A. fruticosa, respectively. In all treatments, root nodule biomass of R. pseudoacacia was significantly higher than those of A. fruticosa. Fertilization significantly increased root nodule biomass for only R. pseudoacacia, however, there were no significant differences in root nodule biomass among fertilization treatments. Root nodule biomass was not influenced by soil nitrogen (N) and phosphorous (P) concentrations following fertilization treatments. Seedling biomass (components and total) and growth (diameter at root collar and height) were strongly correlated with root nodule biomass for the two N fixing tree species.

• 농업생명과학연구
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• 제46권2호
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• pp.63-73
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• 2012

본 연구에서는 소나무 용기묘와 노지묘를 대상으로 조림과정에서 5가지 시비 처리(무시비, 심층시비 20, 50, 100g 및 표층시비 20g)에 따른 생존율, 생장 특성 및 묘목품질지수를 조사 분석하였다. 조림지에 묘목 식재 시 시비처리에 따른 생존율은 처리구별 뚜렷한 차이가 없었으며, 심층시비 100 g 시비를 실시하여도 과량 시비에 의한 피해를 보이지 않았다. 소나무 용기묘와 노지묘의 연차별 근원경과 묘고 생장은 심층시비 100 g에서 가장 높았으며 또한, 물질생산량과 묘목품질지수에서도 생장 특성과 같은 경향이 나타났다. 모든 시비 처리구에서 전반적으로 용기묘가 노지묘보다 우수한 생장을 보였으며, 적정 시비 처리에 따른 효과 또한 용기묘가 노지묘보다 더 높은 것으로 조사되었다.

• 한국초지조사료학회지
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• 제20권2호
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• pp.123-130
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• 2000

This study was carried out to examine the effects of animal manure on efficiency of the nitrogen utilization of silage corn (Zea mays L.) and soil fertility. The experiment was conducted on the field plot at Gongiam, Kwangju, Kyunggi-Do for 3 years, from 1996 to 1998, and arranged in split-plot design with three replications. The main plots were two kinds of composts such as swine manure fermented with sawdust (SMFWS) and swine manure fermented without sawdust (SMF). Subplots were the nitrogen fertilization rate (0, 100, 200, 300 and 400kgNhalyear). The nitrogen (N) yield increased as the nitrogen fertilization rate increased up to a rate of 300 kg Nha, but decreased at rate of 400 kg Nlha. Nitrogen yield in SMF treatments was higher than that of SMFWS treatments. But there were no significant differences between SMFWS and SMF treatments. Organic matter (OM) content of the soils in SMFWS was higher than that of SMF, &d was not significantly different between SMFWS and SMF treatments. OM content increased with increasing the nitrogen fertilization rate. Total nitrogen (TN) content of the soils increased as the nitrogen fertilization rate increased. No difference of TN content was found between SMFWS and SMF treatments. (Key words : Swine manure, Compost, Corn, Sawdust, Nutritive value)

• 생물환경조절학회지
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• 제21권1호
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• pp.57-65
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• 2012

본 연구에서는 시비 처리에 따른 생육상토의 화학적 특성, 생장 특성 및 생리적 특성 변화를 조사 분석하여 최소 시비량에 대한 최대 생장 효과와 적정 시비 방법 적용에 따른 환경오염의 최소화를 목적으로 연구를 실시하였다. 시비 처리에 따른 생육 상토의 화학적 특성은 유의적 차이를 보이지 않았다. 그러나 pH의 경우 유의적 차이를 보였는데 시비량이 적은 Exponential 처리구에서 산성도는 가장 낮고, 시비량이 높은 Constant와 Three stage 처리에서 산성도가 높아, 시비에 따른 산성화가 진행되었음을 짐작할 수 있다. 시비 처리에 따른 근원경과 간장 생장은 유의적 차이를 보이지 않았으며, 세 시비 처리구 모두에서 낙엽송 용기묘 규격 이상의 우수한 생장을 나타냈다. H/D율과 T/R율은 Exponential 처리구에서 가장 낮은 값을 보였으며, 반면 물질생산량과 묘목품질지수는 Constant 처리구가 높은 값을 나타냈다. 시비 처리에 따른 광화학 효율과 엽록소 함량은 Constant 처리구에서 높았지만, 세 처리구 모두 유의적 차이는 보이지 않았다. 본 연구 결과를 종합해 보면, 시비량을 생장에 따라 조절함으로써 시비량을 줄이면서 동시에 비슷한 생장 및 생리적 반응을 보임을 알 수 있었다. Exponential 처리구는 50% 시비량으로 생장, 묘목품질지수 및 생리적특성에서큰차이를 보이지 않았으며, 규격묘 생산이 가능했다. 이는 낙엽송 용기묘 100만본 생산 시 비료량을 약 200kg 이상 줄일 수 있는 것으로, 시설양묘과정에서 다른 시비 방법에 비해 경제적인 효과를 얻을 수 있음과 동시에 양묘장 토양 및 계류수의 오염을 줄이면서 비슷한 생장을 유지할 수 있다. 즉, Exponential 시비 방법은 생산 비용을 줄이면서 비슷한 생장 효과 얻어 경제적이면서 친환경적 시비방법이라 판단된다.

• 한국산림과학회지
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• 제104권1호
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• pp.90-97
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• 2015

본 연구는 주요 활엽수 조림수종인 느티나무, 참느릅나무, 거제수나무 및 피나무를 대상으로 세 가지 시비 처리($0.5g{\cdot}L^{-1}$, $1.0g{\cdot}L^{-1}$, $2.0g{\cdot}L^{-1}$)에 따른 근원경, 간장, 물질생산량, 묘목품질지수 등 생장 특성을 조사 분석하여 시설양묘과정에서 실질적으로 적용 가능한 수종별 적정 시비기술을 구명하고자 수행하였다. 느티나무는 $2.0g{\cdot}L^{-1}$, 거제수나무는 $0.5g{\cdot}L^{-1}$ 시비처리구에서 가장 우수한 생장 및 묘목품질을 나타냈는데, 시비수준에 따른 두 수종간의 생장 특성이 반대의 경향을 보인 것이다. 또한, 시비처리에 따른 참느릅나무와 피나무의 근원경과 간장 생장은 유의적 차이가 없었다. 그러나 묘목품질지수는 두 수종 모두 $0.5g{\cdot}L^{-1}$ 시비처리구에서 가장 낮았으며, $1.0g{\cdot}L^{-1}$과 $2.0g{\cdot}L^{-1}$ 시비처리구 간 유의적 차이는 없었다. 따라서 묘목 품질과 함께 경제적, 환경적 측면을 고려했을 때 느티나무는 최소 $2.0g{\cdot}L^{-1}$, 참느릅나무와 피나무는 $1.0g{\cdot}L^{-1}$, 거제수나무는 최대 $0.5g{\cdot}L^{-1}$이 적정 시비 수준으로 판단된다. 수종별 적정 시비 수준(배액, 회수)의 적용으로 우량 묘목 생산 및 이와 연계된 조림성과 향상뿐만 아니라 환경오염 저감, 생산비용 절감 및 양묘 기간 단축 등 경제적 이점이 기대된다.

• 한국토양비료학회지
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• 제46권6호
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• pp.487-493
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• 2013

The effects of long-term fertilization on soil biological properties and microbial community structure in the plough layer in a rice paddy soil in southern Korea were investigated in relation to the continuous application of chemical fertilizers (NPK), straw based compost (Compost), combination these two (NPK + Compost) for last 40 years. No fertilization plot (Control) was installed for comparison. Though fertilization significantly improved rice productivity over control, the long-term fertilization of NPK and compost combination was more effective on increasing rice productivity and soil nutrient status than single application of compost or chemical fertilizer. All fertilization treatments had shown significant improvement in soil microbial properties, however, continuous compost fertilization markedly increased soil enzyme and microbial activities as compared to sole chemical fertilization. Results of microbial community structure, evaluated by EL-FAME (ester-linked fatty acid methyl esters) method, revealed big difference among Control, NPK, and Compost. However, both Compost and Compost+NPK treatments belonged to the same cluster after statistical analysis. The combined application of chemical fertilizer and organic amendments could be more rational strategy to improve soil nutrient status and promote soil microbial communities than the single chemical fertilizer or compost application.

• 한국환경복원기술학회지
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• 제16권2호
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• pp.105-114
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• 2013

This study was carried out to find growth increasing methods of Rhododendron mucronulatum in Mt. Biseul. 4 treatments(heavy pruning, light pruning, non-pruning+fertilization, non-pruning(control)) were used in the study area with different pruning methods. Growth characteristics of new shoot, number of creation of flower buds and leaf buds in new shoot were analysed. Significant differences were observed among the treatments and stem diameter degree. Diameter, length of new shoots and the number of flower buds, leaf buds per new shoots increased in the pruning treatments. But heavy pruning showed more effective growth increasing way than light pruning. Non-pruning and fertilization treatments were not effective to increase growth of Rhododendron mucronulatum. And pruning at the end of May was the most effective way for growth of new shoot.

• 한국산림과학회지
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• 제100권2호
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• pp.305-313
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• 2011

시비는 시설양묘과정에서 반드시 실시되어야 하지만, 토양 및 계류수 오염의 문제를 야기할 수 있다. 이에 따라 본 연구에서는 시설양묘과정에서 시비량 조절에 따른 친환경적 경제적 시비방법 구명을 위해 Constant rate, Three stage rate 및 Exponential rate 등의 3 가지 시비 처리를 실시하였으며, 시비 처리에 따른 생장 특성 및 광합성, 엽록소 형광반응, 엽록소 함량 등을 조사 분석하였다. 그 결과, 근원경, 간장 및 물질생산량은 Constant 처리에서 가장 우수하였다. 묘목품질지수 또한 같은 경향을 보였지만, 유의적 차이는 나타나지 않았다. Exponential 처리구에서도 50%의 시비량으로 규격묘 생산이 가능했다. 광합성 능력은 세 처리구 모두 우수하였으며, 광화학 효율과 엽록소 함량은 Exponential 처리구에서 유의적으로 가장 낮게 나타났다. Exponential 시비 방법으로 생리 및 생장에 문제없는 규격묘를 생산할 수 있었다. 즉, 최소 비용의 최대 생장 효과와 함께 친환경적 시비 방법이라 할 수 있다.