• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 추계학술발표회
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• pp.296-297
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• 2008

• 한국초지조사료학회지
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• 제6권3호
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• pp.138-144
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• 1986

본(本) 시험(試驗)은 독일(獨逸)의 남부지방(南部地方)인 Bayern Alps 지역(地域)의 2차천이(次遷移) 식생(植生)인 Carex ferruginea 우점(優占) 군락지(群落地)에서 PK-시비(施肥) 및 면양(緬羊) 방목(放牧)에 依(依)한 걸뿌림 초지(草地)의 식생유지(植生維持) 및 자연초지(自然草地)의 개량(改良)을 생태학적(生態學的)인 면(面)에서 연구(硏究)코자 1980년 4월부터 1983년 10월까지 수행(遂行)되었으며 식생변화(植生變化)에 대(對)한 조사(調査) 방법(方法)으로는 Releve-method, Point-Interecept-method 및 Quadrat-Charting-method 등(等)이 이용(利用)되었다. 1. PK-시비(施肥) 및 면양(緬羊) 방목(放牧)에 의(依)해 겉뿌림 초지(草地)의 사료가(飼料價)는 점차(漸次) 증가(增加)되었으며, 시비(施肥)를 하지 않고 방목(放牧)만 실시(實施)한 곳은 초지(草地)의 사료가(飼料價)가 차츰 감소(減少)되었다, 2. 2차천이(次遷移) 식생(植生)인 자연초지(自然草地)의 품종(品種)(초지가(草地價)은 시비(施肥) 및 방목(放牧)에 의(依)해 시험(試驗) 2년차(年次) 가을에 겉뿌림 초지(草地)와 대등(對等)하게 크게 증진(增進)되었으며 시비(施肥)없이 방목(放牧)만 실시(實施)한 자연초지(自然草地)의 사료가(飼料價)는 증가(增加)되지 않았다. 3. 자연초지(自然草地)에서 PK-시비(施肥)의 영향(影響)은 방목(放牧) 보나 우수(優秀)하였으며, 섭초류(涉草類)는 시비(施肥)에 의(依)해 거의 소멸(消滅)되었고, 화본과(禾本科) 및 두과(荳科)는 크게 증가(增加)되었으며, 시비(施肥)를 하지 않고 면양(緬羊) 방목(放牧)만 실시(實施)한 구(區)는 두과목초(荳科牧草)가 점차(漸次) 감소(減少)되었다. 특(特)히 6월과 10월의 섭초(涉草)의 수량(收量) 비율(比率)이 크게 차이(差異)가 있었는데 이것은 재생력(再生力) 결핍(缺乏)에 기인(基因)되었다. 4. PK-시비(施肥) 및 면양(緬羊) 방목(放牧)에 의(衣)해 시험전(試驗前)의 Carex ferruginea 우점(優占) 초지(草地) 군락(群落)은 기름진 산지초지형(山地草地型)인 Alchemillo-Cynosuretum으로 천이(遷移)되었다.

• 한국수정란이식학회지
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• 제12권2호
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• pp.161-169
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• 1997

This study was conducted to compare the insemination time of bovine oocytes and determine the effects of glucose(1.5 mM) on the development of bovine embryos at early cleavage stage. Oocytes were matured for 24 h, followed by exposure to sperm and cultured in modified Tyrode's media drops or with bovine oviduct epithelial cell monolayer prepared in TCM199(BOECM). Insemination time and culture system were varied in each experiment. In experiment 1, to investigate the developmental capacity of bovine embryos after different time of exposure to sperm, bovine ova and sperm were co-incubated for 18, 30 or 54 h, respectively. The development to blastocysts of 30 and 54 h insemination groups were significantly higher(P<0.05) than 18 h group, and in case of blastocysts of cleaved embryos, 30 h group were significantly higher(P<0.05) than other groups. In experiment 2, we investigated the effect of glucose on early bovine embryos. After 18 h insemination, in vitro fertilized oocytes were separated following 3 groups ; G+0, C+24 and C+48. Oocytes of G+0 group were cultured in glucose added Tyrode's medium after fertilization, oocytes in C+24 and C+48 groups were cultured in glucose free Tyrode's medium after fertilization. After 24 h culture, G+24 group was moved to glucose added medium. All oocytes of 3 groups were moved to BOECM after 48 h culture. The rates of cleavage and development to blastocysts in G+0 group were significantly lower than other groups. In experiment 3, we determined the effects of glucose exposure from 8 to 20 h after insemination on the cleavage and development of oocytes. The oocytes in glucose added group had high capacity of cleavage and further development. This study shows that in bovine oocytes, the optimal exposure to sperm is 30 h and glucose exposure to bovine one-cell embryos is detrimental to their first cleavage and further development in vitro but there has no evidence of detrimental effect of glucose(1.5 mM) exposure to bovine embryos over the two-cell stage in vitro.

• 한국약용작물학회지
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• 제14권4호
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• pp.234-238
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• 2006

바위솔 총 페놀 함량은 조사된 빛의 양 및 질소비료의 시비량과 밀접한 관계가 있으며 빛의 양이 증가될수록 질소비료의 시비량이 증가할수록 총 페놀 함량이 증가하는 경향이 관찰되었다. 총 페놀 함량은 여러 가지 조건하에서 인공적으로 재배된 바위솔보다 자연산 바위솔에서 높은 것으로 조사되었다. 자연산의 경우 개화 후의 총 페놀 함량이 개화 전의 총 페놀함량보다 높았다. 바위솔의 항산화능은 바위솔에 조사된 빛의 양 및 질소비료의 시비량과 관계가 있으며 빛의 양 및 질소비료의 시비량이 증가하면 대략적으로 항산화능도 증가하는 것으로 조사되었다. 인산 및 칼륨비료의 시비량과 총페놀 함량과 항산화능 사이에는 별 큰 상관관계가 없는 것으로 관찰되었다.

• 한국환경농학회지
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• 제38권3호
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• pp.119-123
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• 2019

우리나라 노지 밭토양의 질소 비료 추천은 토양 유기물 범위에 따라 차등하여 추천한다. 토양 유기물 함량에 따른 질소 공급 가능량을 확인하기 위하여 노지 고추 재배 밭토양에서 작물 재배 전 토양을 채취하여 70일간 누적 질소 무기화량을 구하였다. 토양 유기물 함량(SOM)과 무기화 될 수 있는 토양 질소(SNM)의 관계식은 '$MSN(kg\;10a^{-1})=0.2933{\ast}SOM(g\;kg^{-1})+0.0897$ ($r^2=0.6224$, P<0.001)'이었다. 토양 유기물 범위별 평균 질소 무기화량은 각각 10.5, 26.6, 83.3, 105.6 mg kg-1으로 토양 유기물 함량이 많을수록 질소 무기화량도 많았으나, 같은 토양 유기물 범위에 속하는 토양이어도 질소 무기화량은 약 3~4.6배 차이가 있었다. 따라서 밭토양 질소 관리를 위해서는 토양 특성에 따른 질소 공급량 예측을 통해 질소 비료를 추천하는 것이 중요하다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권6호
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• pp.739-747
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• 2000

This experiment was conducted to evaluate the influence of dimethyl-sulfoxise (DMSO, 0, 5, 50, 100 and $500{\mu}M$) on the motility and acrosome reaction of the frozen-thawed spermatozoa from 3 different bulls (Bull A, Band C). Also we evaluated the developmental capacity of bovine embryos fertilized in a medium containing DMSO at various concentrations. DMSO had negligible effects on the sperm motility and acrosome reaction in all three bulls. However, the development rates from 2 to 16 cells stage on the 3rd day after insemination with 50, 100 and $500{\mu}M$ DMSO in Bull-B, and up to the blastocyst stage fertilized with 5, 50, 100 and $500{\mu}M$ in Bull-A were significantly higher (p<0.05) than those of control ($0{\mu}M$ DMSO) group from each bull. Furthermore, the rates of blastocysts per cleaved embryos of 5 to $500{\mu}M$ DMSO group in Bull-A and of 5 to $100{\mu}M$ DMSO in Bull-C were also significantly higher (p<0.05) than those for their $0{\mu}M$ groups, respectively. These results indicate that DMSO at micromol level used for in vitro fertilization might stimulate the development of embryos for some bulls.

• 한국수정란이식학회지
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• 제13권2호
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• pp.191-197
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• 1998

Immature nocytes and in VitrO matured Oocytes collected from the slaughtered Korean cattle were frozen slowly with 10% ethylene glycol+5% polyvinyl pyrolidine+0.05M trehalose (l0EPT), 10% ethylene glycol+5% ficoll+0.05M sucrose (1OEFS), or 10% ethylene glycol+5% ficoll+0.05M trehalose (l0EFT) by cell freezer (experiment 1). And also,They were ultra-rapidly frozen with 30% ethylene glycol+10% polyvinyl pyrolidine+0.5M trehalose (3OEPT) or 30% ethylene glycol+18% ficoll+0.5M sucrose (3OEFS) using electron microscope grid (experiment 2). In experiment 1, the cleavage rate was 23.0% when immature oocytes were frozen slowly using various cryoprotectants descrihed above, and 5.1% of cleaved oocytes developed to over morula stage after in Vitro fertilization (IVF). There were no significant differences among these groups. When matured oocytes were frozen slowly, the total cleavage rate was 19.7%, and over morula stage was 3.2%. lOEPT (4.8%) and EFS (4.4%) were slightly more effective than l0EFT (0.0%) for development in vitro. Only in l0EFT treated group, immature oocytes have higher developmental capacity than matured ones, when they were frozen slowly and IVF after thawing. In experiment 2, oocytes were ultra-rapidly frozen using the electron microscope grid with two kind of cryoprotectants described above. In immature oocyte group, the cleavage rate was 13.9% and 5.8% of cleaved oocytes developed to over morula stage after IVF, and in matured group, 25.7 and 7.6%, respectively. There were no significant differences between two kind of cryoprotectants, but in ultra-rapid freezing using electron microscope grid, the efficiency is slightly higher in matured oocyte group.

• 한국수정란이식학회지
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• 제9권3호
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• pp.261-268
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• 1994

Immatured bovine follicular oocytes added with serum, hormones, granulosa cells and bovine oviduct epithelium cells were fertilized in vitro after in vitro maturation. In vitro maturation and early development capacity were examined and IVF-derived embryos were transferred and to recipients and effects of sperm treatment on in vitro capacitation were investigated. The rate of in vitro maturation was improved when they were co-culutred with granulosa cells in the TCM199 medium added with 10% FCS and hormones. The percentage of acrosome reaction was not differed between sperm treatments and sperm of above 25% under-went AR during 30 min preincubation with caffeine and heparin. The cleavage rate of oocytes in vitro fertilized in TCM199 medium added with 10% FCS and hormones, GC or BOEG higher than that in medium with 10% FCS and GC. But the rate was not significantly different between GC and BOEG The cleavage of rate oocytes cultured in medium containing serum, hormones and BOEG was 80.2% and more embryos were developed to Blastocyst (17.3%). The selected embryos were transferred to 9 recipients by surgical or nonsurgical method but did not result in pregnancy.

• Reproductive and Developmental Biology
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• 제39권2호
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• pp.29-36
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• 2015

Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.

• 아시안잔디학회지
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• 제4권1호
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• pp.5-11
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• 1990

Korean Lawn. Zoysia japonica Steud. is the type of a perennial warm-season turfgrass keeping green leaf color for 5 months from May to September in natural condition in Korea. This Studies were carried out to obtain the effects of N-P-K fertilization, regrowth capacity after mowing and chlorophyll contents on prolongation of the green leaf color period in Zoysia japonica Steud turfgrass areas. The results obtained are summarized as follows ; 1.The effects of nitrogen fertilizer application in Zoysia japonica Steud, turfgrass areas were affected on regrowth ability after mowing green color of leaf canopies and chlorophyll contents, respectively(P<.05) 2.A experimental plots which applied 51 g/m$^2$/10 weeks of N$_3$P$_1$K$_1$fertilizer, there were high significant effects on prolongation of the green leaf color period and the plots of phosphorus and potassium had additive effects. 3.Prolongation of the green leaf color period to the last of October was obviously possible by slow degrees in proportation to increment of the fertilizer. However, it is interpreted that more prolongation of the green period is impossible with N-P-K fertilization and irrigation treatments. unless the intermediate turfgrasses between $C_3$ and $C_4$plants must be found and bred.