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http://dx.doi.org/10.12749/RDB.2015.39.2.29

Factors Influencing the Efficiency of In Vitro Embryo Production in the Pig  

Lin, Tao (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Lee, Jae Eun (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Shin, Hyun Young (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Oqani, Reza K. (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Jin, Dong Il (Department of Animal Science & Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University)
Publication Information
Reproductive and Developmental Biology / v.39, no.2, 2015 , pp. 29-36 More about this Journal
Abstract
Pigs are considered an ideal source of human disease model due to their physiological similarities to humans. However, the low efficiency of in vitro embryo production (IVP) is still a major barrier in the production of pig offspring with gene manipulation. Despite ongoing advances in the associated technologies, the developmental capacity of IVP pig embryos is still lower than that of their in vivo counterparts, as well as IVP embryos of other species (e.g., cattle and mice). The efficiency of IVP can be influenced by many factors that affect various critical steps in the process. The previous relevant reviews have focused on the in vitro maturation system, in vitro culture conditions, in vitro fertilization medium, issues with polyspermy, the utilized technologies, etc. In this review, we concentrate on factors that have not been fully detailed in prior reviews, such as the oocyte morphology, oocyte recovery methods, denuding procedures, first polar body morphology and embryo quality.
Keywords
Oocyte recovery; In vitro maturation; In vitro fertilization; Somatic cell nuclear transfer; Pig;
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