• 한국가금학회지
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• 제32권3호
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• pp.171-177
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• 2005

Monascus 배양물의 급여 수준 및 출하 전 급여 기간이 육계 및 육용 오리의 능력과 혈액 및 고기의 콜레스테롤 함량에 미치는 영향을 조사하기 위하여, 실험 1에서는 monacolin-k의 함량이 $0.5\%$인 Monascus 배양물을 시판 육계 사료에 0(C), 0.5(T1), 1.0(T2) 및 $1.5\%$(T3)씩 첨가하여 $2\~6$주령 간에 4주간 급여하는 4처리를 두었으며, 실험 2에서는 대조구(C)와 monacolin-k의 함량이 $0.5\%$인 Monascu 배양물을 시판 육계 사료에 $1.0\%$ 첨가하여 $4\~6$주령(2주간, T1), $2\~6$주령(4주간, T2) 및 $0\~6$주령(6주간, T3)에 급여하는 4 처리를 두었다. 모든 처리에는 3반복을 두었고 반복당 1일령의 육계 16수와 육용오리 10수를 각각$2m{\times}l.5m$의 평사 사육실에 완전임의 배치하여 6주간 실험한 결과는 다음과 같다. 실험 1에서 Monascus 배양물의 급여 수준이 증가할수록 육계의 증체량과 사료 섭취량은 유의적으로 감소하였으며 (P<0.05), 오리는 반대로 증가하는 경향을 보였으나 유의적인 차이는 없었고, 육계와 오리의 사료 요구율은 모두 증가하는 경향을 보였으나 역시 유의적인 차이는 없었다. 6주간의 폐사율은 처리간에 유의성이 인정되었으나(P<0.05)일정한 경향은 보이지 않았다. 육계와 오리의 혈청 콜레스테롤 함량은Monascus배양물의 급여 수준이 증가할수록 유의하게 감소하였으며(P<0.05), 가슴살과 다리살의 콜레스테롤 함량도 감소하는 경향을 보였으나 유의적인 차이는 없었다. 실험 2에서 출하 전 Monascu배양물의 급여 기간이 길어질수록 육계의 증체량(P<0.05)과 사료 섭취 량은 감소하는 경향을 보였으며, 오리는 반대로 증가하는 경향을 보였으나 통계적인 차이는 없었다. 사료요구율은 육계와 오리에서 모두 증가하는 경향을 보였으나 역시 처리간에 유의적인 차이는 없었으며, 폐사율은 오리에서만 유의성이 있었으나(P<0.05) 일관성이 없었다. 육계와 오리의 혈청 중 콜레스테롤 함량은 출하 전 Monascus 배양물의 급여기간이 증가할수록 유의하게 감소하는 경향을 보였으며(P<0.05), 가슴살과 다리살의 콜레스테롤 함량도 감소하는 경향을 보였으나 유의적인 차이는 없었다.

• 농업과학연구
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• 제45권4호
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• pp.677-685
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• 2018

Weaning pigs often face post-weaning challenges such as diarrhea, low feed intake, and body weight (BW) loss which affects the health and economic value of weaning pigs. Interestingly, the use of yeast cultures (YCs) as feed supplements for pigs has increased markedly in recent years. This study evaluated the effects of yeast cultures (Saccharomyces cerevisiae) on the growth performance, fecal score, and nutrient digestibility of weaning pigs. A total of 50 crossed healthy weaning pigs [(Yorkshire ${\times}$ Landrace) ${\times}$ Duroc] with an average BW of $7.46{\pm}1.60kg$ (28 day of age) were used in a 6-week experiment. The experiment was divided into 3 phases (Phase 1, 1 - 2 weeks; Phase 2, 2 - 4 weeks; Phase 3, 4 - 6 weeks). Dietary treatments were as follows: 1) CON: basal diet and 2) CON + 0.50% YC. During phase 1, the average daily gain (ADG) and average daily feed intake (ADFI) were significantly increased (p < 0.05) in the weaning pigs fed YC supplementation diets compared with the weaning pigs fed the CON diet. During phase 3 as well as overall, the gain/feed ratio (G/F) was significantly increased (p < 0.05) in the YC supplementation group compared with the pigs fed the CON diet. In conclusion, the supplementation of YCs in the diet positively affected the growth performance of weaning pigs during the first two weeks after weaning.

• International Journal of Advanced Culture Technology
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• 제12권1호
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• pp.281-286
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• 2024

With the commercialization and full-timeization of the livestock industry, civil complaints continue to increase, and the livestock industry is facing a crisis due to social problems such as odor caused by livestock manure, soil pollution, water pollution, and environmental pollution. In order to increase productivity in livestock farms, the amount of livestock manure generated is increasing due to excessive use of protein feed and high-density breeding environment, and complaints such as odor and water pollution due to management problems are increasing rapidly. Livestock odor has emerged as a serious social problem, and due to growing complaints, conflicts between the Ministry of Agriculture and Forestry and the Ministry of Environment are even causing the livestock industry to lower its status. There is an urgent need for solutions to identify problems in the livestock industry and improve policies. This study aims to develop a " Abelmoschus Manihot Jinhuakui " brand that can improve the intestinal environment of livestock, reduce odors caused by livestock excrement, and improve the productivity of livestock farms in order to improve the increasingly serious odor problem in livestock sites. For Jeju livestock farms, which place more importance on the environment by securing tourists, eco-friendly feed additives were applied to the experiment, the results were derived, and the focus was on solving fundamental problems of odor generation through the development and packaging of feed additive brands. We aim to fundamentally solve the odor problem of domestic livestock farms, secure eco-friendly livestock farms, and contribute to reducing livestock odors and increasing productivity through research results that reduce ammonia levels in each livestock farm.

• Journal of Microbiology and Biotechnology
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• 제16권3호
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• pp.374-380
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• 2006

In this study, glucose, sucrose, and dextrin were found to be better carbon sources for the production of pullulan by Aureobasidium pullulans HP-2001. Maximal production of pullulan with 200 g/l sucrose as a carbon source was 54.2 g/l. The highest yield of pullulan from sucrose was 0.40, when the sugar concentration was 100 g/1. Optimal conditions for the continuous production of pullulan by A. pullulans HP-2001 in a 7-1 bioreactor were determined by studying the effects of composition of feed solution, dilution rate, and concentration of sucrose in the feed solution. Pullulan concentration and productivity with 100 g/l glucose and 2.5 g/l yeast extract were 38.1 g/l and 0.53 g/l h for 72 h, respectively, in a batch culture of A. pullulans HP-2001. When the substituted medium contained 100 g/l sucrose, 2.5 g/l yeast extract, and mineral salts, which is the same composition as the medium for the production of pullulan, the pullulan concentration and productivity were 74.9 g/l and 0.55 g/l h for 120 h, respectively. The production of pullulan at the steady state increased with a dilution rate up to 0.015/h, and its concentration was 78.4 g/l with a weight average molecular weight ($M_w$) of $4.0{\times}10^5$. Unlike a batch culture, however, the decline of the $M_w$ and the number average molecular weight ($M_n$) of pullulan was not found in the continuous culture of A. pullulans HP-2001. When the concentration of sucrose in the feed solution was 200 g/l, 113.5 g/l of pullulan was obtained at the steady state. The steady state was maintained longer in the continuous culture fed with the feed solution containing 200 g/l sucrose than when fed with the feed solutions containing either 100 or 150 g/l sucrose.

• 생명과학회지
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• 제22권3호
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• pp.340-346
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• 2012

가금류 제품에서 항생제의 잔류와 내성균의 출현으로 인해 항생제 대체물질에 대한 연구가 활발히 진행되고 있으며, 특히 생균제와 활성촉진제 또는 이들을 조합한 신바이오틱의 사용이 권장되고 있다. 본 연구는 새로운 생균제 CS61 배양액의 사료첨가 급여가 육계의 성장 및 사료효율에 미치는 영향과 안전성을 평가하여 항생제를 대체할 수 있는 사료첨가제로서의 개발가능성을 알아보기 위해 수행하였다. CS61 배양액은 0, 0.1 및 1%의 용량으로 28일간 사료에 혼합하여 육계에게 급여하였다. 시험결과, 시험물질 처치군에서 부검 시의 체중과 일당증체량이 대조군에 비해 용량의존적으로 증가하였다. CS61 배양액의 사료 내 첨가는 대조군 동물에 비해 사료효율도 개선하는 것으로 나타났다. 반면, 일반증상과 사망률, 부검소견, 혈액학치 및 혈청생화학치에서는 시험물질의 처치와 관련된 독성소견이 관찰되지 않았다. RAW 264.7 세포를 이용한 일산화질소 시험에서 정제된 CS61 펩타이드는 lipopolysaccharide에 유도된 일산화질소 생성을 용량의존적으로 억제하였다. 본 시험결과는 육계에 CS61 배양액의 사료첨가 급여는 항염증효과를 통해 성장과 사료효율을 개선할 수 있음을 보여주며, 사료첨가제로서 CS61 배양액의 유용성과 개발가능성을 시사해 주고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.1011-1014
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• 2008

A total of 335 sows at a commercial operation (Hitch Pork Producers Inc, Guymon, OK) was used to determine dietary effects of yeast culture supplementation ($XPC^{TM}$, Diamond V Mills) on litter performance. Sows were grouped by parity (parity 1 to 12). Pigs within a group were then allotted to treatments. Treatments consisted of: CON (no added yeast culture) and YC (12 and 15 g/d XPC during gestation and lactation, respectively). Sows were housed individually and fed their assigned gestation and lactation diets from d 35 of gestation to d 21 of lactation. Sows were fed 2.0 kg/d during gestation and ad libitum during lactation. Voluntary feed intake was measured daily during lactation. At farrowing, numbers of pigs born total and alive were measured. Weights of litters were measured at birth and weaning on d 21 of lactation. Litter weight gain of the YC treatment was 6.9% greater (p<0.01) than that of the CON. However, voluntary feed intake of sows and litter size did not differ between treatments. This study indicates that dietary yeast culture supplementation benefits sow productivity by improving litter weight gain. At present, it is not confirmed if improved litter weight gain was due to milk production, which remains to be investigated.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1947-1953
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• 2006

There have been a number of studies of methods for recycling animal wastewater to provide new bioresources. In the present work, a marine algal culture medium, designated KEP II, was prepared by adding swine waste (3% v/v) fermented by soil bacteria to a dilution of f/2 culture medium (CT). When Phaeodactylum tricornutum was grown in batch culture in KEP II, the cells lasted long at the exponential phase producing the specific growth rate and biomass; the production of total amino acids and secondary metabolites rose up to 5-fold. It also substantially enhanced the maximum quantum yield of photo system (PS) II of P. tricornutum, greatly increased the level of thylakoid membranes containing PS, and stimulated the production of pyrenoids, including enzymes for $CO_2$ fixation in chloroplasts. KEP II should improve the cost efficiency of industrial mass batch cultures and the value of microalgae for long-term preservation of fresh aquaculture feed as well as production of anticancer and antioxidant agents. Specifically, a low-cost medium for growing the diatoms of aquaculture feed will be economically advantageous.

• Journal of Microbiology and Biotechnology
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• 제19권12호
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• pp.1603-1611
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• 2009

High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

• 한국식생활문화학회지
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• 제28권2호
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• pp.125-134
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• 2013

This study investigated the effect of a health-related lifestyle on knowledge, attitude, control belief and behavior intention toward breast-feeding of male and female university students in order to develop a breast-feeding education program for students of childbearing age. The subjects were 445 university students (125 male and 325 female) residing in eight provinces in Korea. The overall percentage of subjects intending to breast-feed their baby was 80.7% (73.6% of males and 84.2% of females). Overall, 84.2% of the subjects had high concerns about their health status. Scores reflecting a positive attitude, knowledge, and control beliefs toward breast-feeding were significantly higher (p<0.05~p<0.001) in female students, students majoring in medicine, and higher grades. The students who scored high at attitude, knowledge, and control beliefs also had a significantly higher (p<0.001) intention to breast-feed. When we investigated the sub factors of attitudes toward breast-feeding, significantly more students taking nutrient supplements scored high for physiological factors (p<0.05) and health-related factors (p<0.01). Significantly more non-smoking and non-drinking students scored high for health-related factors (p<0.001) and physiological factors (p<0.001), respectively. The intention to breast-feed was found to be significantly higher (p<0.01) in students who did not smoke, but not with other health-related lifestyles. In summary, these results indicate that the majority of university students intend to breast-feed, with students having a positive attitude, higher knowledge, and stronger control beliefs having a higher intention to breast-feed. Although a student's health-related lifestyle affects their attitudes on the physiological and health-related aspects of breast-feeding, only smoking status had an observable connection on the intention to breast-feed. Thus, when developing breast-feeding education programs for students, we recommend efforts to enhance a favorable attitude, knowledge, and control beliefs toward breast-feeding.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.665-673
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• 2012

A balance trial experiment was carried out to evaluate the potential relationship between an enzymatically hydrolyzed yeast (EHY) and yeast culture combined with a live Bacillus subtilis (Bs) on the productive parameters, ileal digestibility, retention of nutrient and energy and villus morphology in broilers. Seventy two 28 d old, Ross B308 male broilers were assigned to a factorial combination of 2 levels of EHY (0 and 1 kg/ton of feed) and 2 levels of Bs (0 and 125 g/ton of feed). The experiment lasted 2 weeks. Several treatment interactions were observed. EHY-fed broilers showed the lowest feed intake and feed conversion ratio whereas Bs-fed broilers showed the highest feed intake and intermediate feed conversion ratio (EHY and BS interaction, p<0.05). Also, EHY-fed broilers had greater ileal digestibility of dry matter (EHY and BS interaction, p<0.01) and energy (EHY and BS interaction, p<0.05) but these responses were counterbalanced by the combination of EHY and Bs. The thickness of the mucosa was similar between the control and EHY-fed broilers, but was lowest when Bs was added alone (EHY and BS interaction, p<0.01). The thickness of the villus was greater in EHY plus Bs-fed broilers, intermediate for the control and lower for Bs or EHY-fed broilers (EHY and BS interaction, p<0.05). The area of the villus was greater in the control and EHY plus Bs-fed broilers (EHY and BS interaction, p<0.05). In addition, EHY-fed broilers showed greater breast yield and nitrogen retention (p<0.01) and ashes digestibility (p<0.05). On the other hand, Bs-fed broilers had greater carcass and breast weight, nitrogen retention, energy excretion and villus height (p<0.05). In summary, EHY and Bs enhanced some growth, carcass and nutrient retention responses, but did not show any synergic relationship in these responses. Opposite to this, the results suggest that the positive effect of EHY on the feed conversion and digestibility of nutrients were counterbalanced by the addition of Bs.