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http://dx.doi.org/10.5713/ajas.2011.11316

Combination of an Enzymatically Hydrolyzed Yeast and Yeast Culture with a Direct-fed Microbial in the Feeds of Broiler Chickens  

Gomez, S. (National Center of Disciplinary Research in Animal Physiology, Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias)
Angeles, M.L. (National Center of Disciplinary Research in Animal Physiology, Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias)
Mojica, M.C. (Private practice. Veracruz)
Jalukar, S. (Vi-Cor, 905 S Caroline Ave (50401) PO Box 1483, Mason City)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.25, no.5, 2012 , pp. 665-673 More about this Journal
Abstract
A balance trial experiment was carried out to evaluate the potential relationship between an enzymatically hydrolyzed yeast (EHY) and yeast culture combined with a live Bacillus subtilis (Bs) on the productive parameters, ileal digestibility, retention of nutrient and energy and villus morphology in broilers. Seventy two 28 d old, Ross B308 male broilers were assigned to a factorial combination of 2 levels of EHY (0 and 1 kg/ton of feed) and 2 levels of Bs (0 and 125 g/ton of feed). The experiment lasted 2 weeks. Several treatment interactions were observed. EHY-fed broilers showed the lowest feed intake and feed conversion ratio whereas Bs-fed broilers showed the highest feed intake and intermediate feed conversion ratio (EHY and BS interaction, p<0.05). Also, EHY-fed broilers had greater ileal digestibility of dry matter (EHY and BS interaction, p<0.01) and energy (EHY and BS interaction, p<0.05) but these responses were counterbalanced by the combination of EHY and Bs. The thickness of the mucosa was similar between the control and EHY-fed broilers, but was lowest when Bs was added alone (EHY and BS interaction, p<0.01). The thickness of the villus was greater in EHY plus Bs-fed broilers, intermediate for the control and lower for Bs or EHY-fed broilers (EHY and BS interaction, p<0.05). The area of the villus was greater in the control and EHY plus Bs-fed broilers (EHY and BS interaction, p<0.05). In addition, EHY-fed broilers showed greater breast yield and nitrogen retention (p<0.01) and ashes digestibility (p<0.05). On the other hand, Bs-fed broilers had greater carcass and breast weight, nitrogen retention, energy excretion and villus height (p<0.05). In summary, EHY and Bs enhanced some growth, carcass and nutrient retention responses, but did not show any synergic relationship in these responses. Opposite to this, the results suggest that the positive effect of EHY on the feed conversion and digestibility of nutrients were counterbalanced by the addition of Bs.
Keywords
Broiler; Enzymatically Hydrolyzed Yeast; Yeast Culture; Bacillus subtilis; Ileal Digestibility; Nutrient Retention;
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Times Cited By KSCI : 1  (Citation Analysis)  (Related Records In Web of Science)
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1 Yang, Y., P. A. Iji, A. Kocher, L. L. Mikkelsen and M. Choct, 2007. Effects of mannanoligosaccharide on growth performance, the development of gut microflora, and gut function of broiler chickens raised on new litter. J. Appl. Poult. Res. 16:280-288.   DOI
2 Yang, Y., P. A. Iji, A. Kocher, E. Thompson, L. L. Mikkelsen and M. Choct. 2008. Effects of mannanoligosaccharide in broiler chicken diets on growth performance, energy utilisation, nutrient digestibility and intestinal microflora. Br. Poult. Sci. 49:186-94.   DOI   ScienceOn
3 Yang, Y., P. A. Iji and M. Choct. 2009. Dietary modulation of gut microflora in broiler chickens: a review of the role of six kinds of alternatives to in-feed antibiotics. World's Poult. Sci. J. 65:97-114.   DOI
4 Zhang, A. W., B. D. Lee, S. K. Lee, K. W. Lee, G. H. An, K. B. Song and C. H. Lee. 2005. Effects of yeast (Saccharomyces cerevisiae) cell components on growth performance, meat quality, and ileal mucosa development of broiler chicks. Poult. Sci. 84:1015-1021.   DOI
5 Schallmey, M., A. Singh and O. P. Ward. 2004. Developments in the use of Bacillus species for industrial production. Can. J. Microbiol. 50:1-17.   DOI   ScienceOn
6 Scheuermann, S. E. 1993. Effect of the probiotic Paciflor (CIP 5832) on energy and protein metabolism in growing pigs. Anim. Feed Sci. Technol. 41:181.   DOI   ScienceOn
7 Sen, S. L. I., J. S. Kim, K. H. Kim, Y. W. Kim, Chou Khong, J. D. Lohakare, E. K. Kim, H. S. Kim, I. K. Kwon and B. J. Chae. 2011. Effect of Supplementation of Bacillus subtilis LS 1-2 grown on citrus-juice waste and corn-soybean meal substrate on growth performance, nutrient retention, caecal microbiology and small intestinal morphology of broilers. Asian-Aust. J. Anim. Sci. 24:1120-1127.   DOI   ScienceOn
8 Stanley, V. G., M. Winsman, C. Dunkley, T. Ogunleye, M. Daley, W. F. Krueger, A. E. Sefton and A. Hinton, Jr. 2004. The impact of yeast culture residue on the suppression of dietary aflatoxin on the performance of broiler breeder hens. J. Appl. Poult. Res. 13:533-539.   DOI
9 Spriet, S. M., J. A. Decuypere and H. K. Henderickx. 1987. Effect of Bacillus toyoi (Toyocerin) on the gastrointestinal microflora, concentration of some bacterial metabolites, digestibility of the nutrients and the small intestinal mean retention time in pigs. Meded. Fac. Landbouwkd. Rijksuniv. Gent. 52:1673.
10 Spring, P., C. Wenk, K. A. Dawson and K. E. Newman. 2000. The effects of dietary mannanoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poult. Sci. 79:205-211.   DOI
11 Sun, X., C. Novak, A. McElroy and T. Sefton. 2004. The use of a drug free feeding program to maintain performance of broiler chickens from 0 to 49 days of age. Proc. XXII World's Poultry Congress, Istanbul, CD-Rom.
12 Yamabhai, M., S. Emrat, S. Sukasem, P. Pesatcha, N. Jaruseranee and B. Buranabanyat. 2008. Secretion of recombinant Bacillus hydrolytic enzymes using Escherichia coli expression systems. J. Biotechnol. 133:50-57.   DOI   ScienceOn
13 Pelicano, E. R. L., P. A. Souza, H. B. A. Souza, A. Oba, F. R. Leonel, N. M. B. L. Zeola and M. M. Boiago. 2004. Productive traits of broiler chickens fed diets containing different growth promoters. Braz. J. Poult. Sci. 6:177-182.
14 Pelicano, E. R. L., P. A. Souza, H. B. A. Souza, A. Oba, M. M. Boiago, N. M. B. L. Zeola, A. M. Scatolini, V. A. Bertanha and T. M. A. Lima. 2005a. Carcass and cut yields and meat qualitative traits of broilers fed diets containing probiotics and prebiotics. Braz. J. Poult. Sci. 7:169-175.
15 Pelicano, E. R. L., P. A. Souza, H. B. A. Souza, D. F. Figueiredo, M. M. Boiago, S. R. Carvalho and V. F. Bordon. 2005b. Intestinal mucosa development in broiler chickens fed natural growth promoters. Rev. Bras. Cienc. Avic. 7:221-1229.   DOI
16 Rosen, G. D. 2007. Holo-analysis of the efficacy of $Bio-Mos^{(x)}$ in broiler nutrition. Br. Poult. Sci. 48:21-26.   DOI   ScienceOn
17 Pelicia, K., A. A. Mendes, E. S. P. B. Saldanha, C. C. Pizzolante, S. E. Takahashi, J. Moreira, R. G. Garcia, R. R. Quinteiro, I. C. L. A. Paz and C. M. Komiyama. 2004. Use of prebiotics and probiotics of bacterial and yeast origin for free-range broiler chickens. Braz. J. Poult. Sci. 6:163-169.
18 Radecki, S. V. and M. T. Yokoyama. 1991. Intestinal bacteria and their influence on swine nutrition. In: Swine Nutrition (Ed. E. R. Miller, E. U. Duane and A. J. Lewis). Boston. 439-447.
19 Ratcliff, J. 2000. Antibiotic bans- a European perspective. Pages 135-152 in: Proceedings of the 47th Maryland Nutrition Conference for Feed Manufacturers. March 22-24.
20 Samanya, M. and K. Yamauchi. 2002. Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. natto. Comp. Biochem. Physiol. 133:95-104.
21 Santoso, U., K. Tanaka and S. Ohtani. 1995. Effect of dried Bacillus subtilis culture on growth and lipogenic enzyme activity in female chicks. Br. J. Nutr. 74:523-529.   DOI   ScienceOn
22 SAS Institute. 1999. SAS/STAT User's Guide, version 8, 4th Edition. SAS Institute Inc, Cary, NC, USA.
23 Loddi, M. M. 2003. Probioticos, prebioticos e acidificante organico em dietas para frangos de corte [tese]. Jaboticabal: FCAV, UNESP.
24 Macari, M. and A. Maiorka. 2000. Funcao gastrintestinal e seu impacto no rendimento avícola. In: Anais da Conferência Apinco de Ciencia e Tecnologia Avicolas. Campinas: FACTA. 2:161-174.
25 Onifade, A. A. and G. M. Babatunde. 1996. Supplemental value of dried yeast in a high-fibre diet for broiler chicks. Anim. Feed Sci. Technol. 62:91-96.   DOI   ScienceOn
26 Miazzo, R. D., M. F. Peralta and M. Picco. 2005. Performance productiva y calidad de la canal en broilers que recibieron levadura de cerveza (S. cerevisiae) (Productive performance and carcass quality in broilers fed yeast (S. cerevisiae)). Revista electronica de veterinaria REDVET, 6:1-9, http:www.veterinaria.org/revistas/redvet.
27 Mul, A. J. and F. G. Perry. 1994. The role of fructo-oligosaccharides in animal nutrition. In: Recent Advances in Animal Nutrition (Ed. P. C. Garnsworthy and D. J. A. Cole). Nottingham University Press, Nottingham, UK. pp. 57-79.
28 Onifade, A., A. Odunsi, G. Babatunde, S. Olonede and E. Muma. 1999. Comparison of the supplemental effects of Saccharomyces cerevisiae and antibiotics in low protein and high fibre diet fed to broiler chickens. Arch. Tiernahr. 52:29-39.   DOI   ScienceOn
29 Opalinski, M., A. Maiorka, F. Dahlke, F. Cunha, F. S. C. Vargas and E. Cardozo. 2007. On the use of a probiotic (Bacillus subtilis - strain DSM 17299) as growth promoter in broiler diets. Rev. Bras. Cienc. Avic. 9:99-103.   DOI
30 Panda, A. K., S. V. Rama Rao, M. V. L. N. Raju and S. R. Sharma. 2006. Dietary supplementacion of lactobacillus sporogenes on performance and serum biochemico-lipid profile of broiler chickens. J. Poult. Sci. 43:235-240.   DOI   ScienceOn
31 Pelicano, E. R. L., P. A Souza, H. B. A. Souza, A. Oba, E. A. Norkus, L. M. Kodawara and T. M. A. Lima. 2003. Morfometria e ultra-estrutura da mucosa intestinal de frangos de corte alimentados com dietas contendo diferentes probioticos. Rev. Port. Cienc. Vet. 98:124-134.
32 Gil-Turnes, C., F. R. Conceicao and J. R. Gil de los Santos. 2007. Bacillus cereus var. toyoi improves feed efficiency and health in animals. Int. J. Prob. Preb. 2:21-28.
33 Hughes, R. J. 2003. Variation in the digestion of energy by broiler chickens. PhD Diss. Univ. Adelaide, Australia.
34 Gomez, S. and M. de L. Angeles. 2011. Effects of an enzymatically hydrolyzed yeast and yeast culture combined with flavomycin and monensin on finishing broiler chickens. Int. J. Poult. Sci. 10:433-439.   DOI
35 Hampson, D. J. 1986. Alterations in piglet small intestinal structure at weaning. Res. Vet. Sci. 40:32-40.
36 Hooge, D. M. 2004. Meta-analysis of broiler chicken pen trials evaluating dietary mannan oligosaccharide, 1993-2003. Int. J. Poult. Sci. 3:163-174.   DOI
37 Iji, P. A. and D. R. Tivey. 1998. Natural and synthetic oligosaccharides in broiler chicken diets. Poult. Sci. 2:129-143.
38 Iji, P. A., A. A. Saki and D. R. Tivey. 2001. Intestinal structure and function of broiler chickens on diets supplemented with a mannan oligosaccharide. J. Sci. Food Agric. 81:1186-1192.   DOI   ScienceOn
39 Jin, L. Z., Y. W. Ho, N. Abdullah and S. Jalaludin. 2000. Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poult. Sci. 79:886-891.   DOI
40 Kim, I. H., J. D. Hancock, R. H. Hines and C. R. Risley. 1993. Effects of cellulase and bacterial feed additives on the nutritional value of sorghum grain for finishing pigs. Kansas Agric. Exp. Sta. Rep. Prog. No. 695:144.
41 Kornegay, E. T. and C. R. Risley. 1996. Nutrient digestibilities of a corn-soybean meal diet as influenced by Bacillus products fed to finishing swine. J. Anim. Sci. 74:799-805.
42 Kumprecht, I. and F. Zobac. 1997. The effect of mannan oligosaccharides in feed mixtures on the performance of broilers. Zivocisna Vyroba, 42:117-124.
43 Clementino Dos Santos, E., A. Soares Teixeira, A. Gilberto Bertechini, R. Tadeu Fonseca de Freitas, P. Borges Rodregues, E. Souza Dias, D. M. Torres, A. Vieira Santos and R. A. Giacometi. 2002. Effect of growth beneficial additives on broiler carcass yield. Proc. Braz. Soc. Anim. Prod. p. 5.
44 Breves, G., C. Walter, M. Burmester and B. Schroder. 2000. In vitro studies on the effects of Saccharomyces boulardii and Bacillus cereus var. toyoi on nutrient transport in pig jejunum. J. Anim. Physiol. Anim. Nutr. 84:9-20.   DOI
45 Cepero, B. R. 2005. Retirada de los antibioticos promotores de crecimiento en la union europea: causas y consecuencias. XII Congreso bienal de la Asociacion Mexicana de Especialistas en Nutricion Animal. Puerto Vallarta, Jal. pp. 1-49.
46 Cera, K. R., D. C. Mahan, R. F. Cross, G. A. Reinhart and R. E. Whitmoyer. 1988. Effect of age, weaning and posweaning diet on small intestinal growth and jejunal morphology in young swine. J. Anim. Sci. 66:574-584.
47 Ferket, P. R., C. W. Parks and J. L. Grimes. 2002. Benefits of dietary antibiotic and mannan oligosaccharide supplementation for poultry. 22 Pages. In: Proc. Multi-State Poult. Feed. Nutr. Conf., Indianapolis, Indiana USA. May 14-16.
48 Fukata, T., K. Sasai, T. Miyamoto and E. Baba. 1999. Inhibitory effects of competitive exclusion and fructooligosaccharide, singly and in combination, on Salmonella colonization of chicks. J. Food Prot. 62:229-233.
49 Gao, J., H. J. Zhang, S. H. Yu, S. G. Wu, I. Yoon, J. Quigley, Y. P. Gao and G. H. Qi. 2008. Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poult. Sci. 87:1377-1384.   DOI   ScienceOn
50 Gao, J., H. J. Zhang, S. G. Wu, S. H. Yu, I. Yoon, D. Moore, Y. P. Gao, H. J. Yan and G. H. Qi. 2009. Effect of Saccharomyces cerevisiae fermentation product on immune functions of broilers challenged with Eimeria tenella. Poult. Sci. 88:2141-2151.   DOI   ScienceOn
51 AOAC, 2002. Official methods of analysis. 17th ed. Association of Official Analytical Chemists. Arlington, VA.
52 Gedek, B. R. 1999. Mode of actions of probiotics in chickens. Proc. XII Eur. Symp. on Poultry Nutrition, Veldhoven, The Netherlands. pp. 83-90.
53 Adejuno, D., O. Onifade, O. Olutunde, A. Shanni-Awal, D. Babatunde, O. Odu, S. Ademola and Q. Adebesi. 1999. The effect of concentration, age and duration of feeding supplemental yeast (Levucel, $SB^{TM}$ in a high-fiber diet on the performance of broiler chicken. Abs. de la 88th. Annual Meeting of the Poultry Science Association, Inc., p. 52.
54 Alves, A., C. M. Guedes, M. J. Gomes, J. L. Mourao and V. C. Pinheiro. 2003. Digstibility and gut development of broiler chickens fed Bio-MOS versus control. Report For Alltech Biotechnology. Universidade de Tras-os-Montes e Alto Douro. Vila Real, Portugal.
55 Bradley, G. L. and T. F. Savage. 1995. The effect of autoclaving a yeast culture of Saccharomyces cerevisiae on turkey poult performance and the retention of gross energy, and selected minerals. Anim. Feed Sci. Technol. 55:1-7.   DOI   ScienceOn
56 Bailey, J. S., L. C. Blankenship and N. A. Cox. 1991. Effect of fructooligosaccharide on Salmonella colonization of the chicken intestine. Poult. Sci. 70:2433-2438.   DOI   ScienceOn