• 제목/요약/키워드: FeZnSOD

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비만 청소년의 SOD 활성도 및 혈청 항산화무기질 농도에 관한 연구 (A Study on SOD Activity and Serum Antioxidant Mineral Concentrations in Obese Adolescents)

  • 이다홍
    • Journal of Nutrition and Health
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    • 제40권1호
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    • pp.41-48
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    • 2007
  • The purpose of this study was to investigate the relationship between obesity and, erythrocyte SOD (superoxide dismutase) activity and serum antioxidant mineral (Fe, Zn, Cu, Mn and Se) concentrations of adolescents. Subjects were assigned to one of two groups such as obese ($BMI{\geq}25$, 32 boys, 24 girls) and normal group (18.5 < BMI < 23, 27 boys, 30 girls) Subjects were evaluated based on anthropometric measurements, 24-hr dietary recalls and blood analysis. The mean age of the total subjects was 13.8 years. The mean weight (p < 0.001), BMI (p < 0.001) and body fat (p < 0.001) of obese were higher than those of normal group. There was no significant difference in nutrient intake between obese and normal groups. SOD activity of obese group was not significantly different from normal groups, in both males and females. However, in the males, serum Cu concentration of obese were significantly lower than those of normal group. In the females, Serum Mn concentration of obese were significantly lower then those of normal group. In the correlation analysis, BMI of the subjects had significantly negative correlations with serum Cu, Zn and Mn. To summarize the results, increase of obesity may lead to decrease of serum antioxidant minerals such as Cu, Zn and Mn.

Characterization of Superoxide Dismutase in Lactococcus lactis

  • Chang, Woo-Suk;So, Jae-Seong
    • Journal of Microbiology and Biotechnology
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    • 제9권6호
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    • pp.732-736
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    • 1999
  • The superoxide dismutase (SOD) in Lactococcus lactis was measured quantitatively and qualitatively under various culture conditions. The L. lactis SOD was induced by oxidative stress. As the concentration of paraquat to produce superoxide radicals increased, the growth of L. lactis decreased with concomitant increase of SOD activity. The SOD activity was found to be growth-phase dependent: when aerobically grown cells entered to the stationary phase, the activity increased gradually until the late stationary phase. From inhibition studies, L. lactis SOD was found to be insensitive to KCN and $H_2O_2$ which are known to inhibit Cu/ZnSOD and FeSOD, respectively. Moreover, as the concentration of manganese in the medium increased, the activity of SOD also increased. These data strongly suggested that L. lactis possessed a single manganese-containing SOD (MnSOD). Finally, a putative sod gene fragment of 510 bp was identified in L. lactis using a polymerase chain reaction (PCR) with degenerate primers designed from the deduced DNA sequences of known SOD genes.

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Rhodobacter sphaeroides D230이 생성하는 산소 유도체에 작용하는 효소의 특성 (Characterization of Enzymes Against Oxygen Derivatives Produced by Rhodobacter sphaeroides D-230)

  • 김동식;이혜주
    • 미생물학회지
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    • 제40권2호
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    • pp.94-99
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    • 2004
  • 산소의 존재 유ㆍ무 등과 같은 배양 환경의 변화에 따라 통성 혐기성 광합성 세균인 Rhodobacter sphaerodes B-230이 만들어내는 산소 유도체에 작용하는 효소의 특성을 조사한 결과 세포내 SOD는 호기적 배양에서는 초기 배양액의 pH가 7일 때, 혐기적 배양에서는pH 8일 때 활성이 높은 반면 세포외 방출 SOD는 두 배양조건에서 모두 약산성인 pH 6에서 활성이 높았다. Catalase는 두 조건 모두 중성 부근에서 최고의 활성을 보였으며, 산성 pH 부위에서는 급격히 활성이 낮아졌다. Mn-SOD의 활성 유도제인 methyl viologen을 첨가했을 때 두 조건 모두에서 성장의 저해를 보였으며, 배지에 철 이온을 첨가하여 배양 하였을 때 호기적 조건에서만 두 배 이상 활성이 증가되었다. 혐기적 조건에서는 전체적인 활성이 낮아 금속이온의 추가적인 첨가에도 더 이상 활성이 유도되지 않았다. Mn-SOD 활성 저해제인 $NaN_3$와 CuZn-SOD활성 저해제인 NaCN를 배양액에 첨가했을 때 NaCN은 두 가지 배양 조건에서 생성되는 SOD 모두를 저해하지 않았으며, $NaN_3$는 혐기적 배양조건에서만 0.3 mM 이상에서 급격한 SOD활성의 저해를 가져왔다. 따라서 Rhodobacter sphaeroides D-230도 혐기적 배양 조건에서 Mn-SOD가 생성되는 것을 확인할 수 있었으며,호기적 조건에서는 Fe-SOD가 생성되는 것을 확인할 수 있었다. Catalase의 활성도 두 가지 배양조건 모두에서 methy1 viologen에 의해 활성이 유도되었으며, NaCN와 $NaN_3$에 의해서 급격히 저해되었다.

The Virulence of Vibrio vulnificus is Affected by the Cellular Level of Superoxide Dismutase Activity

  • Kang, In-Hye;Kim, Ju-Sim;Lee, Jeong-K.
    • Journal of Microbiology and Biotechnology
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    • 제17권8호
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    • pp.1399-1402
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    • 2007
  • The virulence of superoxide dismutase (SOD) mutants of Vibrio vulnificus, as tested by intraperitoneal injection into mice, decreases in the order of sodC mutant, sodA mutant, and sodB mutant lacking CuZnSOD, MnSOD, and FeSOD, respectively. The survival of SOD mutants under superoxide stress also decreases in the same order. The virulence of soxR mutant, which is unable to induce MnSOD in response to superoxide, is similar to that of the sodA mutant, as the survival of the soxR mutant under superoxide stress is similar to that of the sodA mutant. Consistently, the lowered survival of the soxR mutant is complemented not only with soxR but also with sodA. Thus, the virulence of V. vulnificus is significantly affected by the cellular level of SOD activity, and an increase in SOD level through MnSOD induction by SoxR under superoxide stress is essential for virulence.

Effect of Cadmium on Oxidative Stress and Activities of Antioxidant Enzymes in Tomato Seedlings

  • Cho, Un-Haing;Kim, In-Taek
    • The Korean Journal of Ecology
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    • 제26권3호
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    • pp.115-121
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    • 2003
  • Leaves of two-week old seedlings of tomato (Lycopersicon esculentum) were treated with various concentrations (0∼100 M) of $CdCl_2$ for up to 9 days and subsequent growth of seedlings, symptoms of oxidative stress and isozyme activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POX) were investigated. Compared with the non-treated control, Cd exposure decreased biomass but increased Cd accumulation, hydrogen peroxide production and lipid peroxidation as malondialdehyde (MDA) formation in leaves and roots. Further studies on the developmental changes of isozyme activities showed that Fe-SOD, Cu/Zn-SOD and one of three APX isozymes decreased and CAT and one of four POX isozymes increased in leaves, whereas Fe-SOD, one of three POX isozymes and two of four APX isozymes decreased and CAT increased in roots, showing different expression of isozymes in leaves and roots with Cd exposure level and time. Based on our results, we suggest that the reduction of seedling growth by Cd exposure is the oxidative stress resulting from the over production of $H_2O_2$ and the insufficient activities of antioxidant enzymes particularly involved in the scavenging of $H_2O_2$. Further, the decreased activities of SOD and APX isozymes of chloroplast origin, the increased activities of CAT and POX and high $H_2O_2$ contents with Cd exposure might indicate that Cd-induced oxidative stress starts outside chloroplast.

Light-chilling에 의해 유도된 벼 잎에서의 광합성 변화와 항산화 효소의 반응 (Photochemical Damage and Responses of Antioxidant Enzymes in Rice Leaves Induced to Light-Chilling)

  • 구정숙;추연식;이진범
    • 생명과학회지
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    • 제19권4호
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    • pp.442-448
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    • 2009
  • 대부분의 열대 식물은 chilling에 민감하게 반응한다. 대표적 열대 식물인 벼 잎에 대한 light-chilling 처리와 이 후의 회복기(post-chilling) 동안 일어나는 반응들을 알아보았다. Chilling 시 벼 잎에서의 광합성 효율($F_v/F_m$)은 대조구보다 50% 감소하였고, 상대적으로 $H_2O_2$ 양은 48% 증가하였다. 항산화 효소들 중 SOD와 GR 활성은 chilling과 post-chilling 시 증가하였다. 특히 SOD isoforms의 경우 CuZn-SOD와 Mn-SOD 가 발현된 반면 Fe-SOD는 발현되지 않았다. CAT 활성은 chilling 시 감소하였으며, 반면에 APX는 크게 증가하였다. Chilling 시 CAT의 isoforms의 변화를 보면, CAT-2와 -3의 활성이 감소한 것과 대조적으로 post-chilling 시 이들 isoforms의 활성은 증가하였다. 이처럼 APX와 CAT 활성은 벼 잎이 chilling stress를 겪게 될 때 상반되는 변화를 보여주었다.

Efficacy evaluation of novel organic iron complexes in laying hens: effects on laying performance, egg quality, egg iron content, and blood biochemical parameters

  • Jiuai Cao;Jiaming Zhu;Qin Zhou;Luyuan Zhao;Chenhao Zou;Yanshan Guo;Brian Curtin;Fei Ji;Bing Liu;Dongyou Yu
    • Animal Bioscience
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    • 제36권3호
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    • pp.498-505
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    • 2023
  • Objective: This study was conducted to determine the optimal dose of novel iron amino acid complexes (Fe-Lys-Glu) by measuring laying performance, egg quality, egg iron (Fe) concentrations, and blood biochemical parameters in laying hens. Methods: A total of 1,260 18-week-old healthy Beijing White laying hens were randomly divided into 7 groups with 12 replicates of 15 birds each. After a 2-wk acclimation to the basal diet, hens were fed diets supplemented with 0 (negative control, the analyzed innate iron content was 75.06 mg/kg), 15, 30, 45, 60, and 75 mg Fe/kg as Fe-Lys-Glu or 45 mg Fe/kg from FeSO4 (positive control) for 24 wk. Results: Results showed that compared with the negative and positive control groups, dietary supplementation with 30 to 75 mg Fe/kg from Fe-Lys-Glu significantly (linear and quadratic, p<0.05) increased the laying rate (LR) and average daily egg weight (ADEW); hens administered 45 to 75 mg Fe/kg as Fe-Lys-Glu showed a remarkable (linear, p<0.05) decrease in feed conversion ratio. There were no significant differences among all groups in egg quality. The iron concentrations in egg yolk and serum were elevated by increasing Fe-Lys-Glu levels, and the highest iron content was found in 75 mg Fe/kg group. In addition, hens fed 45 mg Fe/kg from Fe-Lys-Glu had (linear and quadratic, p<0.05) higher yolk Fe contents than that with the same dosage of FeSO4 supplementation. The red blood cell (RBC) count and hemoglobin content (linear and quadratic, p<0.05) increased obviously in the groups fed with 30 to 75 mg Fe/kg as Fe-Lys-Glu in comparison with the control group. Fe-Lys-Glu supplementation also (linear and quadratic, p<0.05) enhanced the activity of copper/zinc-superoxide dismutase (Cu/Zn-SOD) in serum, as a result, the serum malonaldehyde content (linear and quadratic, p<0.05) decreased in hens received 60 to 75 mg Fe/kg as Fe-Lys-Glu. Conclusion: Supplementation Fe-Lys-Glu in laying hens could substitute for FeSO4 and the optimal additive levels of Fe-Lys-Glu are 45 mg Fe/kg in layers diets based on the quadratic regression analysis of LR, ADEW, RBC, and Cu/Zn-SOD.

내저온성과 민감성 호박 품종의 저온 스트레스에 대한 항산화효소의 활성 차이 (Changes in the Activities of Antioxidant Enzymes during Chilling Stress in Chilling-Tolerant and Chilling-Sensitive Cultivars of Cucurbita spp.)

  • 강남준;권준국;조용섭;최영하
    • 생물환경조절학회지
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    • 제16권1호
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    • pp.54-61
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    • 2007
  • 저온에 대한 생육 반응이 다른 호박 두 품종 간 항산화효소의 활성 변화를 분석해 본 결과, 저온에 강한 '흑종'의 잎에는 Rm이 0.20인 Mn-SOD와 와 0.52인 Fe-SOD가 주된 밴드였고, 저온에 약한 '재래 13'호의 잎에는 an이 0.20인 Mn-SOD와 0.58인 Cu/Zn-SOD가 주된 밴드였다. 저온 처리 후 10일째에 '흑종'의 잎에는 Rm이 0.58인 Cu/Zn-SOD밴드가 발현되었고, '재래 13호'의 경우 밴드의 밀도가 증가하는 경향을 보였다. APX 밴드 발현 양상은 두 품종 모두 적온 처리에서는 차이가 없었지만, 저온 처리 후 10일경부터 저온에 강한 '흑종'의 잎에서 새로운 APX밴드가 발현되었다. POB밴드의 발현 양상은 품종간에 뚜렷한 차이가 있었는데, 적온 하에서 '흑종'의 잎에서는 4개의 주된 밴드가, '재래 13호'의 잎에서는 한 개의 주된 밴드가 발현되었다. 그러나 저온 처리시 '흑종'의 잎에서는 Rm이 0.36, 0.40 및 0.54인 밴드의 밀도가 급격하게 증가한 반면 '재래 13호'의 잎에서는 Rm이 0.36과 0.54인 밴드의 밀도가 증가하였다. 저온에 대한 내성과 관계없이 두 품종 모두 저온 처리 후 초기에는 SOD, APX 및 POD의 활성이 급격하게 증가하는 경향을 보였지만, 저온 처리 후 기간이 경과할수록 품종간 차이가 뚜렷하였다. '흑종'의 잎에서는 이러한 활성이 지속적으로 유지되었지만, '재래 13호'의 잎에서는 저온 처리 후 5일경부터 급격하게 감소하여 적온 처리구보다 낮은 활성을 보여 저온에 대한 품종간 내성차이를 잘 반영해 주었다.

Effect of 2,4,5-Trichlorobiphenyl (PCB-29) on Oxidative Stress and Activities of Antioxidant Enzymes in Tomato Seedlings

  • Cho, Un-Haing;Sohn, Ji-Young
    • The Korean Journal of Ecology
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    • 제25권6호
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    • pp.371-377
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    • 2002
  • Leaves of two-week old seedlings of tomato (Lycopersicon esculentum) were treated with various concentrations (0, 0.2 and 0.4 $\mu$g/1) of 2,4,5-trichlorobiphenyl (PCB-29) and subsequent growth of seedlings, symptoms of oxidative stress and activities of antioxidant enzymes were investigated. Compared with the non-treated control, foliar application of PCB-29 decreased both biomass and superoxide ($O_2$) radical production but increased hydrogen peroxide production and lipid peroxidation such as malondialdehyde (MDA) formation with increased activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX). Further studies on the isozymes of SOD, peroxidase (POD) and APX showed that all three isozymes of SOD such as Mn-SOD, Fe-SOD and Cu/Zn-SOD, two among four isozymes of POD and all three isozymes of APX were selectively increased in response to PCB. Therefore, we suggest that a possible cause for the reduction of seedling growth by PCB exposure is the oxidative stress including over production of hydrogen peroxide and the selective expression of specific isozymes of some antioxidant enzymes.