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http://dx.doi.org/10.5352/JLS.2009.19.4.442

Photochemical Damage and Responses of Antioxidant Enzymes in Rice Leaves Induced to Light-Chilling  

Koo, Jeung-Suk (Department of Molecular Biology, Dong-eui University)
Choo, Yeon-Sik (Department of Biology, Kyungpook National University)
Lee, Chin-Bum (Department of Molecular Biology, Dong-eui University)
Publication Information
Journal of Life Science / v.19, no.4, 2009 , pp. 442-448 More about this Journal
Abstract
We investigated photooxidation and responses of antioxidant enzymes involved in scavenging reactive oxygen species (ROS) after light-chilling ($4^{\circ}C$) for 2 days and post chilling ($25^{\circ}C$) in rice leaves. Chilling leaves indicated a 50% reduction in photosynthetic efficiency ($F_v/F_m$ ratio) and a 48% increase of $H_2O_2$, respectively, compared to the control group. In comparison with the control, activities of superoxide dismutase (SOD) and glutathione reductase (GR) increased at light-chilling and post-chilling. CuZn-SOD and Mn-SOD among SOD forms were detected in rice leaves, while Fe-SOD was not found. The increase of SOD and GR activity may serve as a basis for defense against chilling injury as it dismutase superoxide generated by light-chilling. Catalase (CAT) activity decreased during light-chilling, while activity of APX showed remarkable increase during light-chilling in rice leaves. Among CAT isoforms analyzed by 10% native PAGE, activities of isoform -2 and -3 were inhibited during light-chilling. From the elevated APX activity and decreased CAT activity, we suggest that these two enzymes show mutual supplementary relationships, indicating different tendency during light-chilling.
Keywords
Chilling; $H_2O_2$; $F_v/F_m$; rice; ROS scavenging enzymes;
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