Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Efficacy evaluation of novel organic iron complexes in laying hens: effects on laying performance, egg quality, egg iron content, and blood biochemical parameters

  • Jiuai Cao (College of Animal Sciences, Zhejiang University) ;
  • Jiaming Zhu (College of Animal Sciences, Zhejiang University) ;
  • Qin Zhou (College of Animal Sciences, Zhejiang University) ;
  • Luyuan Zhao (College of Animal Sciences, Zhejiang University) ;
  • Chenhao Zou (College of Animal Sciences, Zhejiang University) ;
  • Yanshan Guo (College of Animal Sciences, Zhejiang University) ;
  • Brian Curtin (Zinpro Corporation) ;
  • Fei Ji (Zinpro Corporation) ;
  • Bing Liu (College of Animal Sciences, Zhejiang University) ;
  • Dongyou Yu (College of Animal Sciences, Zhejiang University)
  • Received : 2022.03.04
  • Accepted : 2022.08.22
  • Published : 2023.03.01
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Abstract

Objective: This study was conducted to determine the optimal dose of novel iron amino acid complexes (Fe-Lys-Glu) by measuring laying performance, egg quality, egg iron (Fe) concentrations, and blood biochemical parameters in laying hens. Methods: A total of 1,260 18-week-old healthy Beijing White laying hens were randomly divided into 7 groups with 12 replicates of 15 birds each. After a 2-wk acclimation to the basal diet, hens were fed diets supplemented with 0 (negative control, the analyzed innate iron content was 75.06 mg/kg), 15, 30, 45, 60, and 75 mg Fe/kg as Fe-Lys-Glu or 45 mg Fe/kg from FeSO4 (positive control) for 24 wk. Results: Results showed that compared with the negative and positive control groups, dietary supplementation with 30 to 75 mg Fe/kg from Fe-Lys-Glu significantly (linear and quadratic, p<0.05) increased the laying rate (LR) and average daily egg weight (ADEW); hens administered 45 to 75 mg Fe/kg as Fe-Lys-Glu showed a remarkable (linear, p<0.05) decrease in feed conversion ratio. There were no significant differences among all groups in egg quality. The iron concentrations in egg yolk and serum were elevated by increasing Fe-Lys-Glu levels, and the highest iron content was found in 75 mg Fe/kg group. In addition, hens fed 45 mg Fe/kg from Fe-Lys-Glu had (linear and quadratic, p<0.05) higher yolk Fe contents than that with the same dosage of FeSO4 supplementation. The red blood cell (RBC) count and hemoglobin content (linear and quadratic, p<0.05) increased obviously in the groups fed with 30 to 75 mg Fe/kg as Fe-Lys-Glu in comparison with the control group. Fe-Lys-Glu supplementation also (linear and quadratic, p<0.05) enhanced the activity of copper/zinc-superoxide dismutase (Cu/Zn-SOD) in serum, as a result, the serum malonaldehyde content (linear and quadratic, p<0.05) decreased in hens received 60 to 75 mg Fe/kg as Fe-Lys-Glu. Conclusion: Supplementation Fe-Lys-Glu in laying hens could substitute for FeSO4 and the optimal additive levels of Fe-Lys-Glu are 45 mg Fe/kg in layers diets based on the quadratic regression analysis of LR, ADEW, RBC, and Cu/Zn-SOD.

Keywords

Acknowledgement

Our study was funded by the Key Projects of Science and Technology Plan of Zhejiang Province, China (No. 2015C 02022). We thank to Zinpro Corporation for providing the experimental materials (Fe-Lys-Glu).

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