Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
권호 표지

Changes in the Activities of Antioxidant Enzymes during Chilling Stress in Chilling-Tolerant and Chilling-Sensitive Cultivars of Cucurbita spp.

내저온성과 민감성 호박 품종의 저온 스트레스에 대한 항산화효소의 활성 차이

  • 강남준 (원예연구소 시설원예시험장) ;
  • 권준국 (원예연구소 시설원예시험장) ;
  • 조용섭 (원예연구소 시설원예시험장) ;
  • 최영하 (원예연구소 시설원예시험장)
  • Published : 2007.03.30
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Abstract

To determine whether antioxidant enzyme systems are related to chilling tolerance, changes of antioxidant enzyme activities during the chilling stress were determined in the leaves of a chilling-tolerant cultivar (Cucurbita ficifolia, cv. Heukjong) and a chilling-sensitive cultivar (Cucurbita moschata, cv. Jaerae 13). Leaves of chilling-tolerant plant have two major isoforms, Fe-SOD and Mn-SOD, at the Rm values of 0.20 and 0.52, respectively. In leaves of chilling-sensitive plant, two major isozymes of SOD was observed, one isoform is Mn-SOD at the Rm value of 0.20, and the other isoform is Cu/zn-SOD at the nm value of 0.58. When plants were treated with chilling stress, Cu/zn-SOD at the Rm value of 0.58 was newly expressed at 10 days after chilling stress in the chilling-tolerant plants, and density of this band increased at five days after chilling stress in the chilling-sensitive plants. One APX isozyme band was observed in unstressed plants of both cultivars. Under the chilling stress one APX isozyme band was newly expressed at 10 days after chilling stress in the chilling-tolerant cultivar. Significant genotype differences were observed fnr POD isozyme banding patterns such as few main isozyme bands in chilling-tolerant plants, and one band in chilling-sensitive plants. Densities of three POD isozyme bands at the Rm of 0.36, 0.40 and 0.54 increased at 10 days after chilling stress in the chilling-tolerant plants, while two bands at the nm of 0.36 and 0.54 increased at 10 days and 20 days after chilling stress in the chilling-sensitive plants, respectively. Activities of SOD, APX and POD significantly increased during five days after chilling stress in both cultivars. In the chilling-tolerant cultivar, activities of these enzymes were higher in chilling-stressed plant than in unstressed plants. However, activities of these enzymes in the chilling-sensitive cultivar decreased rapidly after five days of chilling stress, and were lower in chilling stressed plants than in unstressed plants.

저온에 대한 생육 반응이 다른 호박 두 품종 간 항산화효소의 활성 변화를 분석해 본 결과, 저온에 강한 '흑종'의 잎에는 Rm이 0.20인 Mn-SOD와 와 0.52인 Fe-SOD가 주된 밴드였고, 저온에 약한 '재래 13'호의 잎에는 an이 0.20인 Mn-SOD와 0.58인 Cu/Zn-SOD가 주된 밴드였다. 저온 처리 후 10일째에 '흑종'의 잎에는 Rm이 0.58인 Cu/Zn-SOD밴드가 발현되었고, '재래 13호'의 경우 밴드의 밀도가 증가하는 경향을 보였다. APX 밴드 발현 양상은 두 품종 모두 적온 처리에서는 차이가 없었지만, 저온 처리 후 10일경부터 저온에 강한 '흑종'의 잎에서 새로운 APX밴드가 발현되었다. POB밴드의 발현 양상은 품종간에 뚜렷한 차이가 있었는데, 적온 하에서 '흑종'의 잎에서는 4개의 주된 밴드가, '재래 13호'의 잎에서는 한 개의 주된 밴드가 발현되었다. 그러나 저온 처리시 '흑종'의 잎에서는 Rm이 0.36, 0.40 및 0.54인 밴드의 밀도가 급격하게 증가한 반면 '재래 13호'의 잎에서는 Rm이 0.36과 0.54인 밴드의 밀도가 증가하였다. 저온에 대한 내성과 관계없이 두 품종 모두 저온 처리 후 초기에는 SOD, APX 및 POD의 활성이 급격하게 증가하는 경향을 보였지만, 저온 처리 후 기간이 경과할수록 품종간 차이가 뚜렷하였다. '흑종'의 잎에서는 이러한 활성이 지속적으로 유지되었지만, '재래 13호'의 잎에서는 저온 처리 후 5일경부터 급격하게 감소하여 적온 처리구보다 낮은 활성을 보여 저온에 대한 품종간 내성차이를 잘 반영해 주었다.

Keywords

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