• Journal of Korean Medicine Rehabilitation
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• v.24 no.3
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• pp.39-50
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• 2014

Objectives The objective of this study is to investigate the protective effects of Banggi-eum (FangchiYin) on the articular cartilage injuries in rat model of osteoarthritis. Methods Articular cartilage injury was induced by injection of monosodium iodoacetate (MIA) (0.25 mg) into both knee joint cavities of rats. Rats were divided into control group (n=8) and Banggi-eum (FangchiYin) group (n=8), which was taken extracts of Banggi-eum (FangchiYin) by orally for 20 days. At the end of the experiment (20 days after MIA injection), gross and histopathological examinations on the articular structures of knee joints were performed. Proteoglycan (PG) content in articular cartilages was analyzed by safranin O staining method. And also, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interleukin-$1{\beta}$ (IL-$1{\beta}$) contents in synovial fluid were measured by ELISA method. Results 1. Grossly, the degree of articular cartilage injury in the Banggi-eum (FangchiYin) group was alleviated compared with the control group. 2. PG content in articular cartilage of the Banggi-eum (FangchiYin) group was increased significantly compared with the control group. 3. Histopathologically, osteoarthritic score of the Banggi-eum (FangchiYin) group was decreased significantly compared with the control group. 4. TNF-${\alpha}$ and IL-$1{\beta}$ content in synovial fluid of the Banggi-eum (FangchiYin) group was increased compared with the control group. But there was no significance. Conclusions On the basis of these results, we suggest that Banggi-eum (FangchiYin) have inhibiting effects on the progression of articular cartilage injury in MIA-induced osteoarthritis model.

• The Korea Journal of Herbology
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• v.22 no.2
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• pp.109-114
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• 2007

Objectives : A morphological classification among Sinomenium acutum, Cocculus trilobus and Aristolochia fangchi was made through microscopic observation. Method : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Result : 1. Fiber bundles of pericycle in S. acutum are like a crescent moon, stone cells were found among fiber bundles, and starch grain and raphides of calcium oxalate were included in parenchyma. 2. C. trilobus has the fiber bundle of pericycle resembling a crescent moon, but does not have the raphidesof calcium oxalate. 3. A. fangchi has the raphides of calcium oxalate, but does not have the fiber bundles of pericycle. 4. Stems and roots of S. acutum arc mostly similar, but only stems have the pith. Conclusion :' C. trilobus and A. fangchi can be distinguished from each other by the fiber bundles of pericycle and the raphides of calcium oxalate.

• Bulletin of the Korean Chemical Society
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• v.34 no.3
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• pp.975-978
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• 2013

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.1164-1167
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• 2003

Aristolochic acid has been known to be a carcinogenic compound and to cause Chinese herbs nephrophathy(CHN). To determine the content of aristolochic acid in various medicinal herbs marketed domestically, samples of Aristolochia fangchi, Aristolochia fructus, Aristolochia radix, Asiasari radix, Saussureae radix, and Akebia caulis were analyzed using TLC and HPLC. The optimal conditions for TLC and HPLC using $C_{18}$ column were established for the quantitative analysis of aristolochic acid. This study showed that Aristolochia fangchi and Aristolochia fructus examined in this study contain aristolochic acid of 3.9 and 2.3 mg/g sample, respectively. In contrast, aristolochic acid was not detected in the other samples.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.136.1-136
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• 2003

Aristolochic acid has been known to be a carcinogenic compound and to cause Chinese herbs nephrophathy. To determine the content of aristolochic acid in various medicinal herbs marketed domestically, samples of Aristolochia fangchi, Aristolochia fructus, Aristolochia radix, Asiasari radix, Saussureae radix, and Akebia caulis were analyzed using TLC and HPLC. The optimal conditions for TLC and HPLC were established for the quantitative analysis of aristolochic acid. This study showed that Aristolochia fangchi and Aristolochia fructus examined in this study contain aristolochic acid of 3.9 and 2.3 mg/g sample, respectively. In contrast, aristolochic acid was not detected in other samples.

• The Korea Journal of Herbology
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• v.28 no.5
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• pp.1-6
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• 2013

Objectives : This study was conducted to identify the original Sinomini Caulis et Rhizoma plant among Stephania tetrandra, Cocculus trilobus, and Aristolochiae fangchi to develop the genetic marker for Sinomini Caulis et Rhizoma. Methods : Sinomenium acutum was identified by the classification and identification committee of the National Center for Standardization of Herbal Medicines. The chloroplast ndhF gene was amplified. We performed sequences alignment analysis of Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi using BioEdit program. The SFR markers designed were consisted of SF01, SR04, and SR05 primers. Results : Many variations of Sinomeni Caulis et Rhizoma are currently commercialized as herbal medicine. We compared the base sequences of the ndhF intergenic space of chloroplast DNA with Sinomenium acutum, Stephania tetrandra, C. trilobus, and A. fangchi. According to the results, it showed that the nucleotide variations were seen in 30 genes of four species. Phylogenetic analysis revealed that 4 species were classified into five groups based on an inter-group divergence in nucleotide sequence of 9%. We developed SFR marker nucleotides enough to authenticate respective species and confirmed its application on the band size at 419 base pair. These sequence differences at corresponding positions were available genetic markers to identity the Sinomeni Caulis et Rhizoma. Conclusions : Base on these results, the ndhF region was effective in distinguishing Sinomini Caulis et Rhizoma The SFR genetic marker was useful for identifying Sinomini Caulis et Rhizoma with other species.

• The Korea Journal of Herbology
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• v.32 no.6
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• pp.49-54
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• 2017

Objectives : Bangki is commonly Sinomeni Caulis et Rhizoma (SC) in Korea. But it often confused with others such as Cocculi Radix (CR), Stephaniae Tetrandrae Radix (SR) and Aristolochiae Fangchi (AR) due to the similarity in herbal names and morphological characteristics. However, because all of these types of Bangi have different healing properties, they need to be differentiated. Methods : A discrimination on external features of original plants and external characteristics of herbal medicines was carried out using visual examination, stereoscope. For the examination of Internal characteristics of herbal medicines, tissues were dyed using fast green FCF, hematoxylin and safranin O, and the features were observed by the microscope. Results : In external morphology of original plants, the original plants of SC and AR were lignum plants and others were herbaceous plants. The leaf blade and the petiole were another discriminative criteria. In external morphology of herbal medicines, SR and AR have powders and others didn't. Also, SC and CR were determined by the dense of the radiation pattern in the cross section. In internal morphology of herbal medicines, SR and AR were distinguished by the dense of Stone cells. Moreover, SC and CR were different in the pattern of medullary ray and vascular bundle. Conclusions : The results above could be used as identification keys of Bangki. Moreover, these identifications might attribute as a fundamental material to further studies like physicochemical pattern analysis and biological reaction.

• Proceedings of the Korea Contents Association Conference
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• 2009.05a
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• pp.988-992
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• 2009

The combination of colors and lighting is one of the most significant techniques to display the spatial artistic effects in movies. The aesthetic perception created by colors and lighting is extremely expressive and infectious. It has become an essential technique to render the atmosphere for the scenes in current movies. Through researching the principles of spatial lighting and the stage lighting colors' application, while comparing and analyzing the movies directed by Kar-wai Wong and Baz Luhrmann, this thesis mainly aims at further clarifying the application technique of colors and lighting in the space of movies.

• Korean Journal of Pharmacognosy
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• v.38 no.2 s.149
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• pp.123-127
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• 2007

Aristolochic acid (AA) included in the plants Aristolochiaceae have been well known to be nephrotoxic and carcinogenic inducer and to cause renal disease such as Chinese Herb Nephropathy (CHN). In this study, we used a high performance liquid chromatopaphy-mass spectrometry (HPLC-MS) under the positive ion detection mode for the quantitative change of aristolochic acid-I and-II (AA-I and AA-II) in Aristolochiaceae (Aristolochia contorta Bunge, Aristolochia debilis Sieb. et Zucc., Aristolochia fangchi Wu), some related plants (Cocculus trilobus De candolle, Inula helenium Linne, Saussurea lappa Clarke), and its prescriptions (防己茯笭湯, 定喘散) with or without processing. Here, the processing methods and prescriptions in oriental medicine were generally used to alleviate toxicity or alter property of herbal medicines. However, the concentrations of AA-I and AA-II were highly determined in processed material extracts rather than unprocessed those, not measured in some related plants. Also, the concentrations of AA-I and AA-II even at the prescriptions mixed the plants of Aristolochiaceae were detected to range from 0.73 to 2.53 ppm. Thus, the present results suggest that the content of AA-I and AA-II contained to plants of Aristolochiaceae was not reduced by the processing methods or prescriptions which can induce the physico-chemical change and pharmacological transformation in traditional herbal medicines.

• The Journal of Korean Medicine
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• v.28 no.1 s.69
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• pp.1-10
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• 2007

Background : There has been renewed interest in complementary alternative medicine around the world far a decade. On the other hand, the issue of safety of herbal medicine in Korea has been continuously questioned. Issues raised have included not only chemical residue or heavy metals caused by pollution, but also the toxicity of herbal medicine itself. Objectives : The purpose of this study was to review some herbal medicines subject to controversy of toxicity and figure out what kind of problems they evidence. Methods : In this review, PubMed was used to search medical journals. Keywords such as 'herb, or herbal medicine', 'safety, or adverse events, or adverse effect, or toxicity, or liver injury' were applied. Journals were selected by four inclusion criteria, as objectives should be human, researchers should be medical doctors, literature written in the English language, and types of journals should be meta-analysis or practice guideline or reviews. Finally, 9 cases from 60 journals were included in this review. Results : We reviewed 13 herbal medicines for controversy of toxicity such as ephedra(Mahuang), Arisolochia fangchi, Aristolochia manshuriensis, licorice, skullcap , germander, Syo-saiko-to(Xiao-chai-hu-tang), Chelidonium majus, mistletoe, Dictamnus dasycarpus and madder. Conclusions : It suggested that 'who it is prescribed for' is one of the most important factors to identify the causes of toxicity in herbal medicine and dietary supplements, under specific conditions in Korea.