• Asian-Australasian Journal of Animal Sciences
• /
• 제23권9호
• /
• pp.1213-1220
• /
• 2010

This experiment evaluated the effects of dietary lysine restriction and energy density on growth performance, nutrient digestibility and meat quality of finishing pigs. A $2{\times}2$ factorial arrangement of treatments was utilized in a randomized complete block (RCB) design, and factor 1 was lysine restriction and factor 2 was energy density. The control diet was formulated to contain 3.265 Mcal of ME/kg, 0.75% lysine in the early-finishing phase and 3.265 Mcal of ME/kg, 0.60% lysine in the late-finishing phase and other nutrients met or exceeded NRC (1998) standards. Compared to the control diet (CON), lysine levels of experimental diets were restricted to 15% (treatment EL, EEL) or 30% (treatment ELL, EELL), whereas energy level of experimental diets was increased by 0.100 or 0.200 Mcal of ME/kg. A total of 100 crossbred pigs ([Yorkshire${\times}$Landrace]${\times}$Duroc), with average initial body weight of $58.47{\pm}1.42\;kg$, were allotted to 5 dietary treatments based on sex and body weight. Each treatment had 5 replicates with 4 pigs (two barrows and two gilts) per pen. ADG, ADFI and feed efficiency were calculated in an 8-week growth trial. In the late finishing period (5-8 weeks), pigs fed ELL or EELL diets had decreased ADG and feed efficiency (p<0.01), however, when the EEL diet was provided, a similar growth performance was observed compared to those fed the CON diet during the whole experimental period (p>0.05). In a metabolic trial, 15 pigs were used to evaluate the effect of dietary lysine restriction and energy density on nutrient digestibility. The digestibility of dry matter, crude fat and crude ash was not improved by restricting dietary lysine or energy density. However, crude protein digestibility was decreased (p<0.05) as dietary lysine was restricted. When dietary lysine was restricted, fecal nitrogen was increased whereas nitrogen retention was decreased. BUN concentration was affected by dietary lysine restriction; treatments ELL and EELL had higher BUN values than other treatments (p<0.01). Carcass characteristics and meat quality were measured when average body weight of pigs reached $107.83{\pm}1.50\;kg$. Treatment ELL had higher last rib backfat depth (p<0.05) than treatment CON, but ELL and EEL did not differ significantly. The ELL and EEL treatments had higher (p<0.05) subjective marbling score than treatment CON. Treatment EEL showed higher longissimus fat content than treatment EL and CON (p<0.01). The results indicated that finishing pigs fed a diet with 15% lysine restriction and 3.465 Mcal of ME/kg energy density had no detrimental effects on growth performance and N utilization, and could achieve substantial increases in marbling and longissimus fat content of pork.

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권8호
• /
• pp.1080-1087
• /
• 2004

A trial with twenty-four newly weaned Awassi lambs (initial body weight=21.5$\pm$0.8 kg) was conducted using a 3$\times$2 factorial design to study the effect of feeding three sources of protein supplements (soybean meal (SBM), sunflower seed meal (SSM), and cottonseed meal (CSM)), either untreated or formaldehyde-treated on the growth performance and carcass traits of Awassi lambs. Lambs were randomly assigned to one of the six diets (4 lambs/treatment diet) and were individually fed for a period of 107 days. Experimental diets were isonitrogenous and isocaloric. Final live weight and average daily gain (ADG) were affected by both source of protein and formaldehyde treatment (undegradable protein). Lambs fed untreated diets had better (p<0.01) daily gain compared to those fed formaldehyde-treated diets. Similarly total feed intake per animal was significantly (p<0.05) affected by protein source and formaldehyde treatment. Formaldehyde treatment caused a significant decrease (p<0.01) in feed intake compared to lambs fed untreated diets. Feed requirement per unit of gain was not affected by formaldehyde treatment during all periods of the experiment except for the second period (the second 28 day period), whereby untreated SBM, SSM and CSM had better feed conversion ratio (FCR) than the treated groups. Source of protein had a moderate effect (p<0.10) on FCR but had a significant effect (p<0.05) on hot and cold carcass weight, digestive tract empty weight and liver weight, with lambs fed SBM having higher values than lambs fed SSM and CSM diets. Supplementation with undegradable protein had a significant effect (p<0.05) on dressing-out percentage (p<0.05), final live weight, and hot and cold carcass weight (p<0.01). The lower values pertain to lambs fed treated diets compared to lambs fed untreated diets. In general, there were no significant differences among all carcass linear dimensions, carcass cut weights and dissected loin tissue weights for both treatments (protein source and formaldehyde treatment). Supplementation with undegradable protein but not the source of protein resulted in significantly higher dissected leg total bone weight (p<0.05), tibia and femur weight (p<0.05), and femur length (p<0.01) at the same carcass weight. Results suggest that the treatment of SBM, SSM and CSM with formaldehyde did not improve efficiency of feed utilization, lamb performance or carcass traits and that the SBM diet resulted in an increase in lamb performance compared to other experimental diets.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권9호
• /
• pp.1320-1325
• /
• 2005

A 2${\times}$3 factorial arrangement of treatments was used to determine the effects of olaquindox and cyadox on immune response of Landrace${\times}$Large-White geld piglets that had been orally given 10$^{10}$ CFU of Escherichia coli (E. coli, O$_{139}$:K$_{88}$). Factors included (1) E. coli inoculation or control, and (2) no antimicrobials, 100 mg/kg olaquindox and 100 mg/kg cyadox in the basal diet respectively. E. coli inoculums were orally administered 7 days after the diets were supplemented with olaquindox and cyadox. The effects of the two antimicrobials were assessed in terms of: (1) average daily gain (ADG), (2) systemic immune response (the number of white blood cells and lymphocytes, leukocyte bactericidal capacity, lymphocyte proliferation response to PHA, immunoglobulin concentrations, and total serous hemolytic complement activity), and (3) intestinal mucosal immunity including the number of intraepithelial lymphocytes (IELs) and immunoglobulin A secreting cells (ASCs) in the intestinal lamina propria. E. coli inoculation reduced ADG (p<0.05) during the period of d 0 to d 14 after the challenge while the antimicrobial supplementations improved ADG (p<0.01) during the experiment. ADG in cyadox-supplemented pigs was higher (p<0.05) than that in olaquindox-supplemented pigs. The antimicrobials decreased IEL and ASC counts in the jejunum and ileum (p<0.01) while E. coli inoculation caused them to increase (p<0.01). Jejunal ASCs in the cyadox-supplemented pigs were lower (p<0.05) than those in the olaquindox-supplemented. E. coli elicited increase (p<0.05) in white blood cell counts, leukocyte bactericidal capacity, lymphocyte proliferation rate, serous IgA concentrations, and serous hemolytic complement activity. The antimicrobials decreased the measured systemic immune parameters, but not significantly (p>0.05). The data suggest that olaquindox and cyadox suppress E. coli-induced immune activation, especially intestinal mucosal immune activation, which may be involved in the observed growth promotion.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권2호
• /
• pp.245-251
• /
• 2008

The effects of feeding diets containing 20% (L) or 30% (H) neutral detergent fiber (NDF) (DM basis) on performance and carcass traits were studied in three breeds of pig, including pure Mong Cai (MC), crossbred Landrace$\times$Yorkshire (LY) and crossbred MC$\times$Yorkshire (F1). The experiment had a factorial design with two factors, breed and diet. Eighteen piglets of each breed ($60{\pm}3days$) were randomly allocated to three treatments: L-L, low fiber diet in both growing and finishing periods; L-H, low and high fiber diet in the growing and finishing period, respectively; and H-H, high fiber diet in both periods. The diets were iso-energetic and iso-nitrogenous within feeding period. The main fibrous ingredients of the diets were rice bran and cassava residue. There were no effects of fiber level on daily dry matter feed intake (DMI), expressed as g/kg metabolic body weight (BW0.75), in both feeding periods (p>0.05). DMI was highest for MC, followed by F1 and LY (p<0.001). Average daily gain (ADG) in L-L and L-H was higher than in H-H in the growing period (p<0.001) and overall (p<0.05), while feed conversion ratio (FCR) was higher in H-H than in L-L and L-H in the growing period (p<0.05) and overall, but no significant differences between treatments were found in the finishing period. In both periods, Landrace$\times$Yorkshire had the highest ADG and the lowest FCR, followed by F1 and Mong Cai (p<0.001). There were no interactions between breed and diet for performance and carcass traits. Carcass and dressing percentage was lower for L-H and H-H than for L-L (p<0.05). There were no significant differences among treatments in back fat thickness and lean meat percentage, or in crude protein and ether extract contents of lean meat. Carcass, dressing and lean meat percentage was highest for LY, lowest for MC and intermediate for F1 (p<0.001). It can be concluded that feeding a high fiber diet in the growing period reduced pig performance, but there was no effect in the finishing period. Pure Mong Cai pigs are not particularly suitable for meat purposes, although the F1 cross with Large White had reasonably good growth performance and carcass quality.

• Asian-Australasian Journal of Animal Sciences
• /
• 제30권4호
• /
• pp.554-561
• /
• 2017

Objective: This experiment was conducted as a $3{\times}2{\times}2$ factorial design to examine the effects of particle size (mean particle size of 331, 640, or $862{\mu}m$), evaluation method (direct vs indirect method) and adaptation duration (7 or 26 days) on the energy content and the apparent total tract digestibility (ATTD) of various chemical components in wheat when fed to finishing pigs. Methods: Forty-two barrows ($Duroc{\times}Landrace{\times}Yorkshire$) with an initial body weight of $63.0{\pm}0.8kg$ were individually placed in metabolic cages and randomly allotted to 1 of 7 diets with 6 pigs fed each diet. For the indirect method, the pigs were fed either a corn-soybean meal based basal diet or diets in which 38.94% of the basal diet was substituted by wheat of the different particle sizes. In the direct method, the diets contained 97.34% wheat with the different particle sizes. For both the direct and indirect methods, the pigs were adapted to their diets for either 7 or 26 days. Results: A reduction in particle size linearly increased the digestible energy (DE) and metabolizable energy (ME) contents as well as the ATTD of gross energy, crude protein, organic matter, ether extract (EE) and acid detergent fiber (ADF) (p<0.05), and had a trend to increase the ATTD of dry matter of wheat (p = 0.084). The DE, ME contents, and ATTD of gross energy, crude protein, dry matter and organic matter were higher (p<0.05) when determined by the direct method, but the ATTD of ADF, EE, and neutral detergent fiber were higher when determined by the indirect method (p<0.05). Prolongation of the adaption duration decreased the ATTD of neutral detergent fiber (p<0.05) and had a trend to increase the ATTD of EE (p = 0.061). There were no interactions between particle size and the duration of the adaptation duration. The ATTD of EE in wheat was influenced by a trend of interaction between method and adaptation duration (p = 0.074). The ATTD of ADF and EE in wheat was influenced by an interaction between evaluation method and wheat particle size such that there were linear equations (p<0.01) about ATTD of ADF and EE when determined by the direct method but quadratic equations (p = 0.073 and p = 0.088, respectively) about ATTD of ADF and EE when determined by the indirect method. Conclusion: Decreasing particle size can improve the DE and ME contents of wheat; both of the direct and indirect methods of evaluation are suitable for evaluating the DE and ME contents of wheat with different particle sizes; and an adaptation duration of 7 d is sufficient to evaluate DE and ME contents of wheat in finishing pigs.

• 농업과학연구
• /
• 제42권4호
• /
• pp.361-368
• /
• 2015

본 연구는 육종가를 이용하여 배치한 거세한우에 고에너지 사료를 급여하여 성장, 도체성적 및 혈액성분의 변화에 대하여 분석을 해보았다. 육종가의 적용은 소고기 고급육이 가능한 개체를 선발하는 방법으로 사용되어져 왔다. 본 연구는 육종가에 의해 고, 저로 나누어진 한우그룹에 고에너지 사료를 급여효과를 알아보기 위해 수행되었다. 고육종가 그룹에서 고에너지 사료를 급여할 경우 도체성적이 높아진다는 가정을 하였다. 연구를 수행하기 위해 26마리의 거세한우를 이용하여 $2{\times}2$ 완전임의배치를 하였다(고에너지, 일반사양 과 고육종가, 저육종가로 배치). 체중, 일당증체량, 건물섭취량, 사료요구율의 결과로 고 육종가 그룹에서 수치적으로 높게 나타났다. 혈청성분분석 결과로 비육후기(20 - 27개월령)의 혈청성분중 포도당과 중성지방 농도가 고육종가 그룹에서 유의적으로 높게 나타났고 이는 도체성적에도 긍정적인 효과를 가져오는 것으로 나타났다. 하지만 선행연구에서 나타났던 혈중 알부민과 인의 성분은 도체등급에 영향을 미치지 않는 것으로 보인다. 도체중 분석에서는 근내지방도가 고육종가 그룹이 수치적으로 높게 나타났으나 유의적인 차이는 없었다. 본 연구의 결과로 특정 혈중대사 성분들 중 포도당과 중성지방과 같은 지방대사에 관여하는 성분들은 비육후기 육종가가 높을수록 높은 농도로 나타났고 이는 혈중 대사성분이 유전적인 영향에 의해서 변화가 된다는 것을 알 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제15권1호
• /
• pp.102-105
• /
• 2002

Seventy-two crossbred pigs weighing an average of 41.5 kg were assigned on the basis of sex, weight and litter to one of four dietary treatments in a factorial (4 treatments${\times}$2 sexes) arrangement. The control diet was based on barley and soybean meal while the experimental treatments consisted of diets in which a portion of the dietary protein was supplied by 20% of a 50:50 blend of extruded ($130^{\circ}C$ for 20 to 25 sec) peas and full-fat canola seed, 20% of a 50:50 blend of unextruded peas and full-fat canola seed or a diet containing 10% peas, 6% canola meal and 4% canola oil (to equal the level of canola oil provided by 10% whole canola seed). Digestibility coefficients for dry matter, crude protein and gross energy were significantly higher (p<0.05) for the control diet than for the other three diets. Extrusion produced no beneficial effects (p>0.05) on nutrient digestibility and there were no differences in digestibility between the diet based on intact canola seed compared with the diet containing canola meal and oil. Choice of protein supplement had no significant effects on gain, feed intake or feed conversion during the grower or finisher phases and over the entire experimental period. Extrusion of the pea-canola blend produced no beneficial effects on pig performance as the performance of pigs fed either the extruded or unextruded blend of peas and canola seed was similar. In addition, the performance of pigs fed diets containing intact canola seed was similar to that of pigs fed canola meal and oil. Castrates gained faster and consumed more feed than gilts (p<0.05). However, their feed conversion was poorer than that of the gilts during the finisher period. There were no significant differences in carcass traits between pigs fed the control and any of the experimental treatments. Extrusion had no effect on carcass traits and the carcasses of pigs fed canola meal and oil did not differ from those of pigs fed whole canola seed. Castrates had a significantly lower dressing percentage, lower estimated lean yield but greater loin fat depth than gilts (p<0.05). The results of this experiment indicate that peas in combination with canola seed or canola meal are an acceptable alternative to soybean meal as a protein supplement for use in growing-finishing swine diets. Extrusion did not appear to have any beneficial effects on the nutritional value of the canola seed-pea blend as nutrient digestibility, growth performance and carcass traits were similar for pigs fed the unextruded blend of peas and canola seed compared with the extruded product. Since the process adds to the cost of the raw products, its use is unlikely to be economical.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권12호
• /
• pp.1784-1793
• /
• 2015

This study was carried out to investigate the effects of tributyrin (TB) on the growth performance, pro-inflammatory cytokines, intestinal morphology, energy status, disaccharidase activity, and antioxidative capacity of broilers challenged with lipopolysaccharide (LPS). A total of 160 one-day-old Cobb broilers were allocated to 1 of 4 treatments, with 4 replicated pens per treatment and 10 birds per pen. The experiment consisted of a $2{\times}2$ factorial arrangements of treatments with TB supplementation (0 or 500 mg/kg) and LPS challenge (0 or $500{\mu}g/kg$ body weight [BW]). On days 22, 24, and 26 of the trial, broilers received an intraperitoneal administration of $500{\mu}g/kg$ BW LPS or saline. Dietary TB showed no effect on growth performance. However, LPS challenge decreased the average daily gain of broilers from day 22 to day 26 of the trial. Dietary TB supplementation inhibited the increase of interleukin-$1{\beta}$ (in the jejunum and ileum), interleukin-6 (in the duodenum and jejunum), and prostaglandin $E_2$ (in the duodenum) of LPS-challenged broilers. Similar inhibitory effects of TB in the activities of total nitric oxide synthase (in the ileum) and inducible nitric oxide synthase (in the jejunum) were also observed in birds challenged with LPS. Additionally, TB supplementation mitigated the decrease of ileal adenosine triphosphate, adenosine diphosphate and total adenine nucleotide and the reduction of jejunal catalase activity induced by LPS. Taken together, these results suggest that the TB supplementation was able to reduce the release of pro-inflammatory cytokines and improve the energy status and anti-oxidative capacity in the small intestine of LPS-challenged broilers.

• 수산해양교육연구
• /
• 제28권3호
• /
• pp.611-618
• /
• 2016

A $3{\times}2$ factorial experiment was conducted to investigate effects of water temperature and feeding rate on growth and body composition of olive flounder. Triplicate groups of fish (initial body weight of 118 g) fed a extruded pellet (55% protein and 4708 cal/g) to satiation and at restricted feeding rates of 0.25 and 0.4% body weight per day (BW/d) at the different water temperatures (13 and $18^{\circ}C$) for 9 weeks. Weight gain increased significantly with increase in feeding rates at each temperature. Weight gain of fish fed to satiation was significantly higher at 18 than $13^{\circ}C$, whereas, that of fish fed at 0.25 and 0.4% BW/d were significantly or slightly lower at 18 than $13^{\circ}C$. Feed efficiency and protein efficiency ratio of fish fed to satiation were not significantly different between 13 and $18^{\circ}C$, but those of fish fed at 0.25 and 0.4% BW/d were significantly higher at 13 than $18^{\circ}C$. The major finding of this study is that satiation feeding is efficient for optimal growth and feed efficiency of grower oliver flounder (116-164 g) in suboptimal water temperatures. The maintenance feeding ration which is zero growth performance, were 0.30 and 0.41% BW/day at 13 and $18^{\circ}C$, respectively. In the restricted feeding regime, compromised growth of fish were worsen in higher water temperature ($18^{\circ}C$ vs. $13^{\circ}C$). It might be related to high metabolic rate of fish that spend more energy for maintenance metabolism. Based on these results, we suggest that a satiation feeding regime is recommended for a productive growth of grower olive flounder in the suboptimal temperature.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권9호
• /
• pp.1339-1347
• /
• 2003

An experiment was conducted to investigate the effects of microbial phytase ($Natuphos^{(R)}$) supplementation in combination with carbohydrases (composed of enzymes targeted to soybean meal (SBM) dietary components such as $\alpha$-galactosides and galactomannans; $Endo-Power^{(R)}$) to corn-soybean meal based diet (CSD) and complex diet (CD) with a partial replacement of SBM with rape seed meal (RSM) and cotton seed meal (CSM) on growth performance and nutrient digestibility of growing pigs. A total of 168 growing pigs averaging $13.18{\pm}1.77kg$ of initial body weight was arranged as a $2{\times}2$ factorial design with main effects of diet types (corn-SBM based diet (CSD) and complex diets (CD; 5% of SBM was replaced with 2.5% of RSM and 2.5% of CSM in diet for phase I (0 to 3 weeks) and 6% of SBM was replaced with 3% of RSM and 3% of CSM in diet for phase II (4 to 7 weeks))) and enzyme supplementation (none and 0.1% of phytase (500 FTU/kg diet) and 0.1% of carbohydrases). The diet with enzyme application were formulated to have a 0.18% unit lower aP than diets without enzyme application. Each treatment had three replicates with 14 pigs per replicate. To determine supplementation effect of phytase and carbohydrases on ileal amino acid digestibility of SBM, RSM and CSM, a total of 18 T-cannulated pigs (initial body weight; $13.52{\pm}1.24kg$) were assigned to six dietary treatments in the present study. Dietary treatments in metabolic trial included 1) SBM diet, 2) SBM diet+with enzymes (phytase (500 FTU/kg) and carbohydrases at 0.1%, respectively), 3) CSM diet, 4) CSM diet+enzymes, 5) RSM diet and 6) RSM diet+enzymes. During whole experimental period (0 to 7 wks), there was no difference in growth performance between diets (CSD and CD). However, dietary phytase and carbohydrases supplementation significantly improved gain/feed ratio (G:F) of growing pigs. During the phase II (4-7 weeks), dietary phytase and carbohydrases supplementation significantly improved all fecal nutrient digestibilities (Dry matter (DM), gross energy (GE), crude protein (CP), crude fat (CF), calcium (Ca) and phosphorus (P)). Dietary phytase and carbohydrases supplementation improved significantly overall ileal amino acid digestibilities of SBM, RSM and CSM based diets (p<0.05). The simultaneous inclusion of phytase and carbohydrases in both of CSD and CD reduced feed cost per kg body weight gain (FCG). Also, results suggest that 2.5 to 3% of RSM and CSM, respectively, might be used as a protein source in growing pig diets without having an adverse effect on the growth performance and nutrient digestibility and simultaneous phytase and carbohydrases addition improves nutritional value of SBM, RSM and CSM by improving ileal amino acid digestibilities.